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Chapter13_Outline
Chapter13_Outline

... There are two essential functions of the cell cycle:  To ensure that each chromosomal DNA molecule is replicated only once per cycle  To ensure that the identical replicas of each chromosome are distributed equally to the two daughter cells ...
Practise Final exam
Practise Final exam

... Wild type AABBCC D Do these data tell us if these genes are linked? NO E What kind of cross would you do with the F1 mice to determine linkage between the different genes? Test cross ...
Lecture6-Chap4 Sept19 - Department Of Biological Sciences
Lecture6-Chap4 Sept19 - Department Of Biological Sciences

MGA 8/e Chapter 12
MGA 8/e Chapter 12

... ESTs define the 5´ and 3´ ends of the transcript. ...
Genome Variant Calling: A sta>s>cal perspec>ve
Genome Variant Calling: A sta>s>cal perspec>ve

... •  for  tumors  copy  number  varies  and  the  varia2on  in  the  genome   tends  to  be  a  func2on  of  the  type  of  cancer  (or  lifestyle:   smoking  induces  G-­‐>T  transversions)  so  reasonable  priors  are   harder  to  ob ...
DNA
DNA

...  DNA degraded to fragments only a few hundred base pairs in length can serve as effective templates for amplification.  Large numbers of copies of specific DNA sequences can be amplified simultaneously with multiplex PCR reactions.  Commercial kits are now available for easy PCR reaction setup an ...
doc - Vanderbilt University
doc - Vanderbilt University

... test tube was amazing. I had no idea that DNA can actually be seen with the naked eye. But it was there, in my little tube exposed by a concoction of chemicals that my shaky pipette had miraculously delivered in the appropriate amount. It was an impossibly thin strand curled and knotted and suspende ...
Answer Key
Answer Key

Protein Synthesis
Protein Synthesis

... a copy of that part of the instructions. a. mRNA will replace the T codon for U b. It will also copy a start and end code. 3. When complete, the mRNA leaves the nucleus to find a ribosome. ...
No Slide Title
No Slide Title

... Meselson and Stahl proposed this Theory stating that each strand of DNA created during replication in part new strand and part original. ...
DNA Packaging
DNA Packaging

... • DNA compaction must be dynamic, because changes in the degree of condensation must occur quickly and when needed, as the cell passes through the stages of the cell cycle. • Furthermore, when in its most highly compacted form, DNA is not accessible to transcription or replication enzymes, so it mus ...
Chapter 1 The Science of Genetics
Chapter 1 The Science of Genetics

... Trait: a notable feature or quality in an individual that makes us unique Allele:an alternative form (versions) of a gene (one of a pair) that occupy a specific position on a specific chromosome. Variation among non-coding DNA sequences. ...
Transcription and Translation Review Lesson Plan
Transcription and Translation Review Lesson Plan

... cellular functions of organisms. ...
Honors Biology
Honors Biology

... 3. Compare and contrast mitosis and meiosis. Know the reason for each type of cell division. How do the cells that result from each process compare to the original cell? 4. Describe the structure of the genetic material (DNA) during each phase of the cell cycle. 5. Know the details of each of the ph ...
Transcription
Transcription

... The structure of a bacterial RNA polymerase. Two depictions of the three-dimensional structure of a bacterial RNA polymerase, with the DNA and RNA modeled in. This RNA polymerase is formed from four different subunits, indicated by different colors (right). The DNA strand used as a template is red, ...
March 13
March 13

... introns are self-splicing (type II): no spliceosomes or other enzymes! 2) mRNA editing:many cp mRNAs differ from the gene encoding them •an ACG is modified post-transcriptionally to a functional AUG start codon in several tobacco mRNAs; many other post-transcriptional changes have also been identifi ...
Honors Biology Mid
Honors Biology Mid

... 3. Compare and contrast mitosis and meiosis. Know the reason for each type of cell division. How do the cells that result from each process compare to the original cell? 4. Describe the structure of the genetic material (DNA) during each phase of the cell cycle. 5. Know the details of each of the ph ...
Bio 2 final n
Bio 2 final n

... d. they maintain the genetic code by preventing incorrect DNA base pairings. e. they correct enzymatic alterations of DNA bases. ____ 38. A mutation in which of the following parts of a gene is likely to be most damaging to a cell? a. intron b. exon c. 5' UTR d. 3' UTR e. All would be equally damagi ...
Types of plasmid One way of grouping plasmids is by their ability to
Types of plasmid One way of grouping plasmids is by their ability to

... Plasmids serve as important tools in genetics and biochemistry labs, where they are commonly used to multiply (make many copies of) or express particular genes. There are many plasmids that are commercially available for such uses. Initially, the gene to be replicated is inserted in a plasmid. These ...
Bulletin - Sigma
Bulletin - Sigma

... (LA) PCR combines a highly processive thermostable polymerase with a second thermostable polymerase that exhibits a 3’→5’ exonucleolytic activity. This blend increases the length of amplification products by using the proofreading polymerase to repair terminal misincorporations. This repair allows t ...
genetics ppt review
genetics ppt review

... MEIOSIS for a FRUIT FLY: ...
The Dolan DNA Learning Center at Cold Spring Harbor Laboratory
The Dolan DNA Learning Center at Cold Spring Harbor Laboratory

... 2. What role does –galactosidase play in lactose metabolism? It breaks down lactose (a disaccharide) into galactose and glucose (monosaccharides). 3. What is the inducer of the lac operon? __Lactose or allolactose__ (note: cAMP is an activator) 4. What role does the repressor (product of the lacI g ...
Proc 16(4) Oct 03 web.indd
Proc 16(4) Oct 03 web.indd

Genomics for the Rancher: How Does it Work and What
Genomics for the Rancher: How Does it Work and What

... has the base uracil (U) rather than thymine (T) that is present in DNA. Chromosomes A chromosome is an organized structure of DNA and protein found in cells. It is a single piece of coiled DNA containing many genes, regulatory elements and other nucleotide sequences. Chromosomes also contain DNA-bou ...
Nerve activates contraction
Nerve activates contraction

... • Almost all of the DNA in a eukaryotic cells is subdivided into chromosomes in the nucleus. • Tiny amounts of DNA are found in mitochondria and chloroplasts. ...
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Cre-Lox recombination



In the field of genetics, Cre-Lox recombination is known as a site-specific recombinase technology, and is widely used to carry out deletions, insertions, translocations and inversions at specific sites in the DNA of cells. It allows the DNA modification to be targeted to a specific cell type or be triggered by a specific external stimulus. It is implemented both in eukaryotic and prokaryotic systems.The system consists of a single enzyme, Cre recombinase, that recombines a pair of short target sequences called the Lox sequences. This system can be implemented without inserting any extra supporting proteins or sequences. The Cre enzyme and the original Lox site called the LoxP sequence are derived from bacteriophage P1.Placing Lox sequences appropriately allows genes to be activated, repressed, or exchanged for other genes. At a DNA level many types of manipulations can be carried out. The activity of the Cre enzyme can be controlled so that it is expressed in a particular cell type or triggered by an external stimulus like a chemical signal or a heat shock. These targeted DNA changes are useful in cell lineage tracing and when mutants are lethal if expressed globally.The Cre-Lox system is very similar in action and in usage to the FLP-FRT recombination system.
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