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Creation/Evolution - Geoscience Research Institute
Creation/Evolution - Geoscience Research Institute

... from some other source In bacteria, restriction enzymes are paired with methylases that recognize the same sequences Restriction enzymes will not cut methylated DNA Thus restriction endonucleases cut up foreign DNA, but not the cell’s DNA Working with methylases, REs restrict bacteriophages to ...
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SUNY-ESF Web

... site of a ribosome and causes premature chain termination during translation. This antibiotic looks like the 3’ end of the aminoacylated tRNA and will affect both prokaryotes and eukaryotes. . Rifamycin-class of antibiotics produced by Streptomyces that inhibit prokaryotic but not eukaryotic RNAPs ...
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... b. exon = coding region of DNA / RNA (exons are expressed) Alternative splicing (removing different combinations of introns and exons from a given gene) allows for efficiency and diversity. Consider: each gene contains about 20 times the number of base pairs necessary for a functional protein produc ...
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heredity (b)

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Biology Final Exam Study Guide (FULL)

... *Sex is an inherited phenotypic character usually determined by which sex chromosomes are present. Humans and other mammals have an X-Y system in which sex is determined by whether a Y chromosome is present. *Sex chromosomes can carry genes from some traits that are unrelated to sex characteristics ...
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official course outline information

... 4. Understand the biochemistry and biophysics of a symbiotic relationship. 5. Isolate and clone DNA from a marine bacterium which encodes proteins responsible for bioluminescence. 6. Manipulate DNA using recombinant DNA technology, including restriction analysis, agarose gel electrophoresis, ligatio ...
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Learning Intentions

...  I understand that food contains chemical energy that can be converted into ATP in cells.  I know the structure of ATP, understand that ATP is constantly being used to do cellular work and is constantly being regenerated.  I can state cells with a high energy requirement (such as muscle, sperm, n ...
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Cre-Lox recombination



In the field of genetics, Cre-Lox recombination is known as a site-specific recombinase technology, and is widely used to carry out deletions, insertions, translocations and inversions at specific sites in the DNA of cells. It allows the DNA modification to be targeted to a specific cell type or be triggered by a specific external stimulus. It is implemented both in eukaryotic and prokaryotic systems.The system consists of a single enzyme, Cre recombinase, that recombines a pair of short target sequences called the Lox sequences. This system can be implemented without inserting any extra supporting proteins or sequences. The Cre enzyme and the original Lox site called the LoxP sequence are derived from bacteriophage P1.Placing Lox sequences appropriately allows genes to be activated, repressed, or exchanged for other genes. At a DNA level many types of manipulations can be carried out. The activity of the Cre enzyme can be controlled so that it is expressed in a particular cell type or triggered by an external stimulus like a chemical signal or a heat shock. These targeted DNA changes are useful in cell lineage tracing and when mutants are lethal if expressed globally.The Cre-Lox system is very similar in action and in usage to the FLP-FRT recombination system.
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