• Study Resource
  • Explore Categories
    • Arts & Humanities
    • Business
    • Engineering & Technology
    • Foreign Language
    • History
    • Math
    • Science
    • Social Science

    Top subcategories

    • Advanced Math
    • Algebra
    • Basic Math
    • Calculus
    • Geometry
    • Linear Algebra
    • Pre-Algebra
    • Pre-Calculus
    • Statistics And Probability
    • Trigonometry
    • other →

    Top subcategories

    • Astronomy
    • Astrophysics
    • Biology
    • Chemistry
    • Earth Science
    • Environmental Science
    • Health Science
    • Physics
    • other →

    Top subcategories

    • Anthropology
    • Law
    • Political Science
    • Psychology
    • Sociology
    • other →

    Top subcategories

    • Accounting
    • Economics
    • Finance
    • Management
    • other →

    Top subcategories

    • Aerospace Engineering
    • Bioengineering
    • Chemical Engineering
    • Civil Engineering
    • Computer Science
    • Electrical Engineering
    • Industrial Engineering
    • Mechanical Engineering
    • Web Design
    • other →

    Top subcategories

    • Architecture
    • Communications
    • English
    • Gender Studies
    • Music
    • Performing Arts
    • Philosophy
    • Religious Studies
    • Writing
    • other →

    Top subcategories

    • Ancient History
    • European History
    • US History
    • World History
    • other →

    Top subcategories

    • Croatian
    • Czech
    • Finnish
    • Greek
    • Hindi
    • Japanese
    • Korean
    • Persian
    • Swedish
    • Turkish
    • other →
 
Profile Documents Logout
Upload
Lectures 1. Meiosis and Recombination in yeast. After this lecture
Lectures 1. Meiosis and Recombination in yeast. After this lecture

... A) Genes names are italicized and have three letters. -e.g. CDC stands for Cell Division Cycle, URA stands for URAcil biosynthesis. C) The three letters are followed by a number, which typically represent different genes that share a common phenotype. -> The genes involved in uracil biosynthesis are ...
DETERMINATION OF NUCLEOTIDE SEQUENCES IN DNA
DETERMINATION OF NUCLEOTIDE SEQUENCES IN DNA

... FREDERICK SANGER Medical Research Council Laboratory of Molecular Biology, Cambridge, England ...
Ch18WordLectureOutli..
Ch18WordLectureOutli..

...  The emergence of these new viral diseases is due to three processes: mutation, spread of existing viruses from one species to another, and dissemination of a viral disease from a small, isolated population.  Mutation of existing viruses is a major source of new viral diseases.  RNA viruses tend ...
CHEM642-14 Powerpoint
CHEM642-14 Powerpoint

... cells was first fractionated by allowing it to percolate through an ion-exchange resin packed into a column (A). The column was washed, and the bound proteins were then eluted by passing a solution containing a gradually increasing concentration of salt onto the top of the column. Proteins with the ...
Standardized Test Prep Gene Technologies and Human Applications
Standardized Test Prep Gene Technologies and Human Applications

... Student essays should be supported by facts and logical arguments. Consider peer review of essays. Sample argument: No, such information might create biases against those whose diseases are well-researched as opposed to those about which less is known. Genetic research should not be abused because t ...
Note 7.5 - Genetic Mutations
Note 7.5 - Genetic Mutations

... Insertion – is the addition of a base pair (small-scale mutation) or larger coding region (large-scale mutation) to a DNA sequence. Deletion – is the removal of a base pair (small-scale mutation) or larger coding region (large-scale mutation) from a DNA sequence. Inversion – is two adjacent bases tr ...
Yellow Line Walk-through
Yellow Line Walk-through

... and Arabidopsis have been exhaustively studied for TEs. Prospecting other genomes will reveal new information about these organisms. ...
2420 Topics for Examination II
2420 Topics for Examination II

... size? How is DNA transferred from the agarose gel to a special filter membrane? What is a Southern blot? Is bacterial probe DNA able to distinguish human target DNA from bacterial target DNA? Know that this specificity is due to base sequences which have unique homologies, preventing attachment of p ...
New KS3 Year 9 Medium Plan
New KS3 Year 9 Medium Plan

... All students will recall that the nucleus contains genes that control the characteristics of the organism Most students will be able to describe the process of fertilisation Some students will explain the process of fertilisation using appropriate terminology ...
Minor Groove to Major Groove, an Unusual DNA Sequence
Minor Groove to Major Groove, an Unusual DNA Sequence

Recombinant DNA cloning technology
Recombinant DNA cloning technology

... Has a chromosome with a ‘left’ arm and a ‘right’ arm, that contain all the genes needed for lysis. Between two arms, there is a disposable segment since it does not contain any lytic cycle genes. These two regions, the arms and the disposable region is separated by EcoRI sites. The lambda chromosome ...
Final Exam - brownscience
Final Exam - brownscience

... 63. There are ___ types of neurons 64. Which system coordinates the body’s response to changes in its internal and external environment? 65. Neurons are classified by the 66. What is the smallest structural and functional unit of the nervous system? 67. What begins when a neuron is stimulated by ano ...
Macromolecule Review Guide
Macromolecule Review Guide

... 7. Describe the structure of glucose (elements, shape, formula, etc). ...
Lecture 13
Lecture 13

... genomic DNA extracted from the transgenic plants. A thermostable DNA polymerase amplifies the region between the two primers during the multiple amplification cycles of the PCR, which yields a DNA fragment of predicted size (the length equal to the number of base pairs between the two primers in the ...
Who are you? This question can be answered many ways…
Who are you? This question can be answered many ways…

... Individuals with Sickle Cell Anemia have oddly shaped red blood cells. – The shape of the mutated protein allows the cell to bind to itself, changing the cells shape. – The shape of the red blood cell doesn’t allow it to flow easily through the body, disabling the individual. ...
DNA Extraction from Gram negative bacteria on plates and
DNA Extraction from Gram negative bacteria on plates and

The maintenance of sex in bacteria is ensured
The maintenance of sex in bacteria is ensured

... The maintenance of sex in bacteria is ensured by its potential to reload genes Gergely J. Szöllősi1 , Imre Derényi1 and Tibor Vellai2 Keywords: Genome evolution, Evolution of recombination, Genome organization dynamics Sexual reproduction is a process that brings genomes, or portions of genomes, ...
Chapter 15 Genetic Engeneering
Chapter 15 Genetic Engeneering

... • Certain points between the genes on the DNA have repeating base sequences. – For example: ATTACGCGCGCGCGCGCGCTAGC – These are called variable number tandem repeats (VNTRs for short) ...
DNA and Genetics Review
DNA and Genetics Review

... c. egg cell and sperm cell b. egg cell only d. body cell and egg cell Completion Complete each sentence or statement. 23. The plants that Mendel crossed to produce the F1 generation made up the ____________________ generation. 24. A pea plant that has two different alleles for the same trait is said ...
IS91 transposase is related to the rolling-circle
IS91 transposase is related to the rolling-circle

... site specificity [GAAC/CAAG] (1,2). IS97 is related to IS807 from Pseudomonas syringae (3). In particular, their transposases are 35% identical proteins of 426 and 410 amino acids respectively (2,3). Apart from this, IS91 is unrelated to other presently known IS elements. Figure 1 shows the four con ...
14-3 Human Molecular Genetics
14-3 Human Molecular Genetics

07Lab_MitoMei - Biology Learning Center at the University of
07Lab_MitoMei - Biology Learning Center at the University of

... •A gene is ~1,000-100,000 basepairs* •A chromosome is tens or hundreds of thousands of genes ...
GENETIC ENGINEERING - CAPE Biology Unit 1 Haughton XLCR …
GENETIC ENGINEERING - CAPE Biology Unit 1 Haughton XLCR …

... helix producing "blunt" ends. However, many restriction enzymes cut in an offset fashion. • The ends of the cut have an overhanging piece of single-stranded DNA. These are called "sticky ends" because they are able to form base pairs with any DNA molecule that contains the complementary sticky end. ...
Promoters - Pennsylvania State University
Promoters - Pennsylvania State University

... Free Probe Lane ...
Watermarking sexually reproducing diploid organisms
Watermarking sexually reproducing diploid organisms

... be encrypted into DNA is first modified by a mutation correction code, the Hamming-code, to correct mutations within the DNA sequences. A header, containing the length of the original file, is produced and linked to the modified binary sequence. The composition of the header and the file is translat ...
< 1 ... 361 362 363 364 365 366 367 368 369 ... 766 >

Cre-Lox recombination



In the field of genetics, Cre-Lox recombination is known as a site-specific recombinase technology, and is widely used to carry out deletions, insertions, translocations and inversions at specific sites in the DNA of cells. It allows the DNA modification to be targeted to a specific cell type or be triggered by a specific external stimulus. It is implemented both in eukaryotic and prokaryotic systems.The system consists of a single enzyme, Cre recombinase, that recombines a pair of short target sequences called the Lox sequences. This system can be implemented without inserting any extra supporting proteins or sequences. The Cre enzyme and the original Lox site called the LoxP sequence are derived from bacteriophage P1.Placing Lox sequences appropriately allows genes to be activated, repressed, or exchanged for other genes. At a DNA level many types of manipulations can be carried out. The activity of the Cre enzyme can be controlled so that it is expressed in a particular cell type or triggered by an external stimulus like a chemical signal or a heat shock. These targeted DNA changes are useful in cell lineage tracing and when mutants are lethal if expressed globally.The Cre-Lox system is very similar in action and in usage to the FLP-FRT recombination system.
  • studyres.com © 2026
  • DMCA
  • Privacy
  • Terms
  • Report