Importance of integrons in the diffusion of resistance
... Integrase (IntI) is a member of the tyrosine site-specific recombinase family, which also includes the well-known λ-phage integrase. These enzymes catalyse the excision and integration of DNA units, by performing two consecutive strand breakage and rejoining steps. These steps take place without DNA ...
... Integrase (IntI) is a member of the tyrosine site-specific recombinase family, which also includes the well-known λ-phage integrase. These enzymes catalyse the excision and integration of DNA units, by performing two consecutive strand breakage and rejoining steps. These steps take place without DNA ...
Multiplex RT-PCR kit.
... NOTE: It is essential for functionality of the HemaVision-12;21 kit also to obtain and use the reagents provided in HemaVision-RMP kit, Cat. No. HV06-RMP containing: 20 µL MMLV-Reverse Transcriptase, 100 µL 5x cDNA buffer, 50 µL DTT, 100 µL dNTP, 45 µL HemaVision DNA Polymerase, 300 µL 10x PCR ...
... NOTE: It is essential for functionality of the HemaVision-12;21 kit also to obtain and use the reagents provided in HemaVision-RMP kit, Cat. No. HV06-RMP containing: 20 µL MMLV-Reverse Transcriptase, 100 µL 5x cDNA buffer, 50 µL DTT, 100 µL dNTP, 45 µL HemaVision DNA Polymerase, 300 µL 10x PCR ...
Maintenance of genomic integrity by p53: complementary
... described above could be one such mechanism, but other interactions, mediated by the N-terminal prolinerich domain of p53 which serves as a docking place for proteins containing SH3-domains, have also been implicated. Even less understood is what determines the `decision' to induce either a growth a ...
... described above could be one such mechanism, but other interactions, mediated by the N-terminal prolinerich domain of p53 which serves as a docking place for proteins containing SH3-domains, have also been implicated. Even less understood is what determines the `decision' to induce either a growth a ...
Cloning: A Critical Analysis of Myths and Media
... criminal investigations. It is worth noting that DNA cloning technology is also used in genetic engineering efforts, such as transgenic technology (where DNA from one species is inserted into another) and/or gene pharming (where scientists alter an animal’s DNA so that it can make human proteins, dr ...
... criminal investigations. It is worth noting that DNA cloning technology is also used in genetic engineering efforts, such as transgenic technology (where DNA from one species is inserted into another) and/or gene pharming (where scientists alter an animal’s DNA so that it can make human proteins, dr ...
Genetic or epigenetic difference causing discordance between
... cannot be explained by changes in DNA sequence.12 For such mechanisms, methylation of the cytosine residue in the DNA molecule, and acetylation, methylation, and other modifications of histones have been well described. These modifications stably affect gene expression through alteration of DNA– pro ...
... cannot be explained by changes in DNA sequence.12 For such mechanisms, methylation of the cytosine residue in the DNA molecule, and acetylation, methylation, and other modifications of histones have been well described. These modifications stably affect gene expression through alteration of DNA– pro ...
Finishing the Human Genome
... Doug Brutlag Professor Emeritus of Biochemistry & Medicine Stanford University School of Medicine Doug Brutlag 2011 ...
... Doug Brutlag Professor Emeritus of Biochemistry & Medicine Stanford University School of Medicine Doug Brutlag 2011 ...
A-level Biology Previous essay titles and mark schemes
... 3. Nutrient cycles, the organisms involved 3. Succession, biodiversity, species and individuals in a community 4. Human impacts on the environment and its effect on relationships between organisms – including farming 4. Gene technology and GMO and ...
... 3. Nutrient cycles, the organisms involved 3. Succession, biodiversity, species and individuals in a community 4. Human impacts on the environment and its effect on relationships between organisms – including farming 4. Gene technology and GMO and ...
Science Flashcard Printouts.doc
... Alfred Sturtevant’s linkage maps were based on this scientist’s research on inheritance. ...
... Alfred Sturtevant’s linkage maps were based on this scientist’s research on inheritance. ...
Nucleic acid hybridisation and polymerase chain reaction
... - absence of polypurines or polypirimidines - absence of secondary structure - absence of complementarity, particularly at the 3 ' end, which may p r o m o t e the formation of an artifact called "primer dimer". Computer programmes, available upon request, can help to perform this task (62). Since t ...
... - absence of polypurines or polypirimidines - absence of secondary structure - absence of complementarity, particularly at the 3 ' end, which may p r o m o t e the formation of an artifact called "primer dimer". Computer programmes, available upon request, can help to perform this task (62). Since t ...
WW Genetic Counselor English - Wonderwise
... Meet Cathy Burson, genetic counselor. Cathy works in a hospital clinic, where she sees people of all ages. Adults and kids visit her with different kinds of health problems that might be passed on from one generation to the next. You probably know that we inherit features such as our hair or eye col ...
... Meet Cathy Burson, genetic counselor. Cathy works in a hospital clinic, where she sees people of all ages. Adults and kids visit her with different kinds of health problems that might be passed on from one generation to the next. You probably know that we inherit features such as our hair or eye col ...
Aucun titre de diapositive - Universidad Nacional De Colombia
... Any resulting cluster without a polyadenylation signal or at least two 3’ ESTs is discarded. The resulting clusters are called anchored clusters since their 3’ end is supposedly known. ...
... Any resulting cluster without a polyadenylation signal or at least two 3’ ESTs is discarded. The resulting clusters are called anchored clusters since their 3’ end is supposedly known. ...
Molecular Biology and Applied Genetics
... process that are studying in vivo, but it doesn’t necessarily tell how direct that role is. Biochemistry, by contrast, tells what a factor can do in vitro, but it doesn’t necessarily mean that it does it in vivo. The genetic and biochemical approaches tell you different things: Genetics ...
... process that are studying in vivo, but it doesn’t necessarily tell how direct that role is. Biochemistry, by contrast, tells what a factor can do in vitro, but it doesn’t necessarily mean that it does it in vivo. The genetic and biochemical approaches tell you different things: Genetics ...
Chapter 11 Powerpoint
... • 2. All have a significant number of unusual bases made by altering normal base posttranscriptionally • 3. All have base sequences in one part of molecule that are complementary to those in other parts • 4. Thus, all fold in a similar way to form cloverleaf-like structure (in 2 dimensions) • 5. Ami ...
... • 2. All have a significant number of unusual bases made by altering normal base posttranscriptionally • 3. All have base sequences in one part of molecule that are complementary to those in other parts • 4. Thus, all fold in a similar way to form cloverleaf-like structure (in 2 dimensions) • 5. Ami ...
Dissecting plant meiosis using Arabidopsis thaliana mutants
... cells (a mitosis-like division). Agashe et al. (2002) report that in the dyad mutant GUS (b-glucuronidase) expression is driven from a DMC1 promoter during female meiosis suggesting that this division may be meiotic rather than mitotic in nature. Subsequently, these cells can undergo either a second ...
... cells (a mitosis-like division). Agashe et al. (2002) report that in the dyad mutant GUS (b-glucuronidase) expression is driven from a DMC1 promoter during female meiosis suggesting that this division may be meiotic rather than mitotic in nature. Subsequently, these cells can undergo either a second ...
Simplified Insertion of Transgenes Onto Balancer Chromosomes via
... RMCE was supported at appreciable levels using at least one representative target-bearing line for each of the three balancer chromosomes, namely FM7hFS5, CyOJ01, CyOJ08, and TM3FS18. In the presence of a genomic source of the phiC31 integrase (Bischof et al. 2007), we injected donor constructs carr ...
... RMCE was supported at appreciable levels using at least one representative target-bearing line for each of the three balancer chromosomes, namely FM7hFS5, CyOJ01, CyOJ08, and TM3FS18. In the presence of a genomic source of the phiC31 integrase (Bischof et al. 2007), we injected donor constructs carr ...
Characterization of the Role of Eco1 in Chromosome
... chromatids (Figure 2). During the Synthesis (S) phase, the genetic material is replicated, forming sister chromatids. After replication, each of the chromosomes has a duplicate copy, now existing as a complex of two identical sister chromatids. These identical sister chromatids must remain tightly t ...
... chromatids (Figure 2). During the Synthesis (S) phase, the genetic material is replicated, forming sister chromatids. After replication, each of the chromosomes has a duplicate copy, now existing as a complex of two identical sister chromatids. These identical sister chromatids must remain tightly t ...
GENtle, a free multi-purpose molecular biology tool
... suffers from severe drawbacks. Free software exists, but is often hard to set up and operate for users on today’s point-and-click interfaces, and usually leads to the application of a patch-work of multiple, only partially compatible tools and web services. Commercial software often covers only part ...
... suffers from severe drawbacks. Free software exists, but is often hard to set up and operate for users on today’s point-and-click interfaces, and usually leads to the application of a patch-work of multiple, only partially compatible tools and web services. Commercial software often covers only part ...
INFINITI CYP450 2C19+ Assay
... xenobiotics metabolism. CYP450 2C19 (CYP2C19) is a highly polymorphic liver enzyme of the liver and is involved in the metabolism and elimination of many commonly prescribed drugs including antidepressants, antiepileptics, barbiturates and proton pump inhibitors. Genetic polymorphisms in CYP2C19 are ...
... xenobiotics metabolism. CYP450 2C19 (CYP2C19) is a highly polymorphic liver enzyme of the liver and is involved in the metabolism and elimination of many commonly prescribed drugs including antidepressants, antiepileptics, barbiturates and proton pump inhibitors. Genetic polymorphisms in CYP2C19 are ...
A set reduction and pattern matching problem motivated by Allele
... box) allowing extension of the primers by DNA polymerase. 1) After one iteration the process has created two copies of the original sequence (navy boxes). One copy begins with the left primer, and the other begins with the right primer. 2) A second iteration doubles the number of sequences by duplic ...
... box) allowing extension of the primers by DNA polymerase. 1) After one iteration the process has created two copies of the original sequence (navy boxes). One copy begins with the left primer, and the other begins with the right primer. 2) A second iteration doubles the number of sequences by duplic ...
1: Summary and Options
... in the apparent absence of any unusual stimuli, or they can be “induced” by particular agents. It is likely that many or most “spontaneous” mutations are caused by external forces, possibly in- ...
... in the apparent absence of any unusual stimuli, or they can be “induced” by particular agents. It is likely that many or most “spontaneous” mutations are caused by external forces, possibly in- ...
the x chromosome in population genetics
... X chromosome makes it an ideal system for studying population-genetic differences between males and females, particularly the differences in mutation rate and in patterns of recombination. The presence of a single copy of the X chromosome in males means that X-linked alleles are more exposed to natu ...
... X chromosome makes it an ideal system for studying population-genetic differences between males and females, particularly the differences in mutation rate and in patterns of recombination. The presence of a single copy of the X chromosome in males means that X-linked alleles are more exposed to natu ...
Cre-Lox recombination
In the field of genetics, Cre-Lox recombination is known as a site-specific recombinase technology, and is widely used to carry out deletions, insertions, translocations and inversions at specific sites in the DNA of cells. It allows the DNA modification to be targeted to a specific cell type or be triggered by a specific external stimulus. It is implemented both in eukaryotic and prokaryotic systems.The system consists of a single enzyme, Cre recombinase, that recombines a pair of short target sequences called the Lox sequences. This system can be implemented without inserting any extra supporting proteins or sequences. The Cre enzyme and the original Lox site called the LoxP sequence are derived from bacteriophage P1.Placing Lox sequences appropriately allows genes to be activated, repressed, or exchanged for other genes. At a DNA level many types of manipulations can be carried out. The activity of the Cre enzyme can be controlled so that it is expressed in a particular cell type or triggered by an external stimulus like a chemical signal or a heat shock. These targeted DNA changes are useful in cell lineage tracing and when mutants are lethal if expressed globally.The Cre-Lox system is very similar in action and in usage to the FLP-FRT recombination system.