Heuris`c)search:)FastA)and)BLAST)
... An algorithm was developed which facilitates the search for similarities between newly determined amino acid sequences and sequences already available in databases. Because of the algorithm's efficiency on many microcomputers, sensitive protein database searches may now become a routine procedure fo ...
... An algorithm was developed which facilitates the search for similarities between newly determined amino acid sequences and sequences already available in databases. Because of the algorithm's efficiency on many microcomputers, sensitive protein database searches may now become a routine procedure fo ...
Point mutations in BCL6 DNA-binding domain reveal distinct
... We show here by point mutation analysis that the six ZFs of BCL6 are not equivalent in DNA-binding activity. Similar findings were reported for the POK proteins ZF5 and Kaiso [12 and 13]. The described experiments differed from our work though, as the ZF region of these two proteins was not examined ...
... We show here by point mutation analysis that the six ZFs of BCL6 are not equivalent in DNA-binding activity. Similar findings were reported for the POK proteins ZF5 and Kaiso [12 and 13]. The described experiments differed from our work though, as the ZF region of these two proteins was not examined ...
K - Romanian Biotechnological Letters
... variant we developed proved to have a very good separation up to 2760 kbp, the largest chromosome of K. lactis, without using a thermostat or buffer recirculation. Unlike other Pulsed Field Gel Electrophoresis (PFGE) techniques, FIGE is supposed to present the so called “inversion band” [18]. Due to ...
... variant we developed proved to have a very good separation up to 2760 kbp, the largest chromosome of K. lactis, without using a thermostat or buffer recirculation. Unlike other Pulsed Field Gel Electrophoresis (PFGE) techniques, FIGE is supposed to present the so called “inversion band” [18]. Due to ...
The nucleotide sequence of Saccharomyces cerevisiae
... Large-scale systematic sequencing has generally depended on the availability of an ordered library of large-insert bacterial or viral genomic clones for the organism under study. The generation of these large insert libraries, and the location of each clone on a genome map, is a laborious and time-c ...
... Large-scale systematic sequencing has generally depended on the availability of an ordered library of large-insert bacterial or viral genomic clones for the organism under study. The generation of these large insert libraries, and the location of each clone on a genome map, is a laborious and time-c ...
lntraclonal mating in Trypanosoma brucei is
... types, determined by loci which encode or control production of specific pheromones ; such systems ensure that mating occurs only between organisms of different ...
... types, determined by loci which encode or control production of specific pheromones ; such systems ensure that mating occurs only between organisms of different ...
Preferential X-chromosome inactivation, DNA
... methylation in the initiation and specificity of inactivation. This specificity (or choice) of which X chromosome will be active or inactive is affected by a number of factors. These influencing factors may also result in differential allele expression in general, i.e. whether the region concerned i ...
... methylation in the initiation and specificity of inactivation. This specificity (or choice) of which X chromosome will be active or inactive is affected by a number of factors. These influencing factors may also result in differential allele expression in general, i.e. whether the region concerned i ...
How were introns inserted into nuclear genes?
... which has homology to reverse transcriptase3~. It is not known whether this is due to an additional endonuclease domain, or whether the reverse-transcriptaselike domain itself could have endonuclease activity; unfortunately, endonucleases cannot always be recognized by homology alone. If introns do ...
... which has homology to reverse transcriptase3~. It is not known whether this is due to an additional endonuclease domain, or whether the reverse-transcriptaselike domain itself could have endonuclease activity; unfortunately, endonucleases cannot always be recognized by homology alone. If introns do ...
CBSE XII BIO QS with key(2009-2015)
... they have food reserves for the seedling or embryo the seed coat provides protection to the embryo generate genetic variation remain viable for a considerable period of time.(any two) =1x2 State what is apomixis? Comment on its significance. How can it be commercially used? (2015) Form of asexual re ...
... they have food reserves for the seedling or embryo the seed coat provides protection to the embryo generate genetic variation remain viable for a considerable period of time.(any two) =1x2 State what is apomixis? Comment on its significance. How can it be commercially used? (2015) Form of asexual re ...
A plant dialect of the histone language
... HMT family [22]. Histone methylation currently focuses considerable research interest because it is associated with transcriptional regulation and methyl transferases contain well conserved domains among different organisms. In contrast to other modifications, no histone demethylases have thus far b ...
... HMT family [22]. Histone methylation currently focuses considerable research interest because it is associated with transcriptional regulation and methyl transferases contain well conserved domains among different organisms. In contrast to other modifications, no histone demethylases have thus far b ...
Chromosome numbers in female and male gametes: One
... exchanges in region 2 and one exchange in (1 ) would represent the remaining 12%. The tetrad frequencies calculated on the basis of first or second division doubling may be compared with those derived from G1, LghA,/gl, lgaal heterozygotes (RHOADESand DEMPSEY 1966) where the Gl-Lg interval is compar ...
... exchanges in region 2 and one exchange in (1 ) would represent the remaining 12%. The tetrad frequencies calculated on the basis of first or second division doubling may be compared with those derived from G1, LghA,/gl, lgaal heterozygotes (RHOADESand DEMPSEY 1966) where the Gl-Lg interval is compar ...
Chapter 1 - Institut Montefiore
... • The human genome consists of about 3 ×109 base pairs and contains about 30,000 genes • Much of the DNA is either in introns or in intragenic regions • Cells containing 2 copies of each chromosome are called diploid (most human cells). Cells that contain a single copy are called haploid. • Humans h ...
... • The human genome consists of about 3 ×109 base pairs and contains about 30,000 genes • Much of the DNA is either in introns or in intragenic regions • Cells containing 2 copies of each chromosome are called diploid (most human cells). Cells that contain a single copy are called haploid. • Humans h ...
North Kitsap High School PAL Program
... what a gene pool is, state what determines how a phenotype is expressed, explain how natural selection affects single-gene and polygenic traits, describe genetic drift, list five conditions needed to maintain genetic equilibrium, identify the conditions necessary for a new species to evolve. (17 sec ...
... what a gene pool is, state what determines how a phenotype is expressed, explain how natural selection affects single-gene and polygenic traits, describe genetic drift, list five conditions needed to maintain genetic equilibrium, identify the conditions necessary for a new species to evolve. (17 sec ...
Sequence Heterogeneities Among 16s
... Sequences were obtained for the seven rrn operons of E. coli strain PK3. These data demonstrated differences located in three highly variable domains. Their nature and localization suggest that since the divergence of E. coli and SaZmoneZZutyphimurium, most point mutations that occurred within each ...
... Sequences were obtained for the seven rrn operons of E. coli strain PK3. These data demonstrated differences located in three highly variable domains. Their nature and localization suggest that since the divergence of E. coli and SaZmoneZZutyphimurium, most point mutations that occurred within each ...
Maintenance of DNA Methylation during the Arabidopsis Life Cycle
... detected transcripts from active postzygotic transcription of the FIS2 maternal allele in the endosperm. A putative general paternal silencing has been observed for reporters of endospermexpressed genes for up to 4 DAP (Vielle-Calzada et al., 2000). It could be hypothesized that such a general mecha ...
... detected transcripts from active postzygotic transcription of the FIS2 maternal allele in the endosperm. A putative general paternal silencing has been observed for reporters of endospermexpressed genes for up to 4 DAP (Vielle-Calzada et al., 2000). It could be hypothesized that such a general mecha ...
Widespread expression of the bovine Agouti gene results from at
... Agouti coding sequences. These results show that the Agouti gene is expressed in all examined bovine tissues including skin regardless of their coat colour. Northern hybridizations on bovine tissues were conducted to detect more accurately Agouti transcripts. A probe corresponding to the entire codi ...
... Agouti coding sequences. These results show that the Agouti gene is expressed in all examined bovine tissues including skin regardless of their coat colour. Northern hybridizations on bovine tissues were conducted to detect more accurately Agouti transcripts. A probe corresponding to the entire codi ...
2. Assessing the probative value of DNA evidence
... jurisdictions. It goes without saying that the laws of probability, unlike the laws of the land, are valid irrespective of geography. It would be artificial and sometimes misleading when describing criminal litigation to avoid any reference whatsoever to legal precepts and doctrines, and we have not ...
... jurisdictions. It goes without saying that the laws of probability, unlike the laws of the land, are valid irrespective of geography. It would be artificial and sometimes misleading when describing criminal litigation to avoid any reference whatsoever to legal precepts and doctrines, and we have not ...
Persistence and Loss of Meiotic Recombination
... Guillon and de Massy 2002; Carrington and Cullen 2004). Furthermore, biased conversion in hotspot heterozygotes has also been detected in human and mouse meioses (Jeffreys and Neumann 2002; Yauk et al. 2003). As predicted by the DSBR model, the less-active hotspot allele preferentially replaces the ...
... Guillon and de Massy 2002; Carrington and Cullen 2004). Furthermore, biased conversion in hotspot heterozygotes has also been detected in human and mouse meioses (Jeffreys and Neumann 2002; Yauk et al. 2003). As predicted by the DSBR model, the less-active hotspot allele preferentially replaces the ...
A survey of denitrifying Azospirillum brasilense in two contrasted
... that only a small percentage of bacteria can be cultivated as yet, the MPN for denitrifiers may represent only a small percentage of bacteria, including the overall denitrifiers. Comparing the two regions of sampling, A. brasilense isolates with denitrifying activity was predominant in the West area ...
... that only a small percentage of bacteria can be cultivated as yet, the MPN for denitrifiers may represent only a small percentage of bacteria, including the overall denitrifiers. Comparing the two regions of sampling, A. brasilense isolates with denitrifying activity was predominant in the West area ...
A genome-wide analysis of DNA methylation in buccal - VU-DARE
... average methylation levels, more variation between subjects, and higher heritability compared to promoter regions in adipose tissue 34. To summarize, there is great interest in unraveling the factors that contribute to variation in DNA methylation between persons, but most previous twin studies of D ...
... average methylation levels, more variation between subjects, and higher heritability compared to promoter regions in adipose tissue 34. To summarize, there is great interest in unraveling the factors that contribute to variation in DNA methylation between persons, but most previous twin studies of D ...
Assessing the Probative Value of DNA Evidence
... jurisdictions. It goes without saying that the laws of probability, unlike the laws of the land, are valid irrespective of geography. It would be artificial and sometimes misleading when describing criminal litigation to avoid any reference whatsoever to legal precepts and doctrines, and we have not ...
... jurisdictions. It goes without saying that the laws of probability, unlike the laws of the land, are valid irrespective of geography. It would be artificial and sometimes misleading when describing criminal litigation to avoid any reference whatsoever to legal precepts and doctrines, and we have not ...
Revista agronomica del Noroeste Argentino
... that only a small percentage of bacteria can be cultivated as yet, the MPN for denitrifiers may represent only a small percentage of bacteria, including the overall denitrifiers. Comparing the two regions of sampling, A. brasilense isolates with denitrifying activity was predominant in the West area ...
... that only a small percentage of bacteria can be cultivated as yet, the MPN for denitrifiers may represent only a small percentage of bacteria, including the overall denitrifiers. Comparing the two regions of sampling, A. brasilense isolates with denitrifying activity was predominant in the West area ...
Jigsaw handout - the Biology Scholars Program Wiki
... binds to lac repressor and causes a conformation change that alters the structure of lac repressor so that it cannot bind to DNA. Therefore, in the presence of lactose, lac repressor does not bind to the operator and RNA polymerase can transcribe the genes in the lac operon. Case study (homework or ...
... binds to lac repressor and causes a conformation change that alters the structure of lac repressor so that it cannot bind to DNA. Therefore, in the presence of lactose, lac repressor does not bind to the operator and RNA polymerase can transcribe the genes in the lac operon. Case study (homework or ...
Cre-Lox recombination
In the field of genetics, Cre-Lox recombination is known as a site-specific recombinase technology, and is widely used to carry out deletions, insertions, translocations and inversions at specific sites in the DNA of cells. It allows the DNA modification to be targeted to a specific cell type or be triggered by a specific external stimulus. It is implemented both in eukaryotic and prokaryotic systems.The system consists of a single enzyme, Cre recombinase, that recombines a pair of short target sequences called the Lox sequences. This system can be implemented without inserting any extra supporting proteins or sequences. The Cre enzyme and the original Lox site called the LoxP sequence are derived from bacteriophage P1.Placing Lox sequences appropriately allows genes to be activated, repressed, or exchanged for other genes. At a DNA level many types of manipulations can be carried out. The activity of the Cre enzyme can be controlled so that it is expressed in a particular cell type or triggered by an external stimulus like a chemical signal or a heat shock. These targeted DNA changes are useful in cell lineage tracing and when mutants are lethal if expressed globally.The Cre-Lox system is very similar in action and in usage to the FLP-FRT recombination system.