A fost luat în studiu caracterul multifoliolar deoarece acest caracter
... From the F1 generation a plant (F1-M8), showing strong multileaflet character, was chosen to perform the bulk segregating analysis. Of the different types of molecular markers that have been developed for Medicago species two types were chosen for this study: RAPD (Random Amplified polymorphic DNA), ...
... From the F1 generation a plant (F1-M8), showing strong multileaflet character, was chosen to perform the bulk segregating analysis. Of the different types of molecular markers that have been developed for Medicago species two types were chosen for this study: RAPD (Random Amplified polymorphic DNA), ...
The evolution of sex chromosomes in organisms with separate
... It is currently not clear how well the theory developed for X/Y and Z/W systems applies to the U/V system. For one, the evolution of recombination per se and the effect of events such as fusions and inversions on the linkage between sex-specific genes and the sex-determining locus in haploid sex chr ...
... It is currently not clear how well the theory developed for X/Y and Z/W systems applies to the U/V system. For one, the evolution of recombination per se and the effect of events such as fusions and inversions on the linkage between sex-specific genes and the sex-determining locus in haploid sex chr ...
DNA
... DNA: the building blocks of genetic material • DNA was later discovered to be the molecule that makes up the inherited genetic material • Experiments performed by Fredrick Griffith in 1928 and experiments with bacteriophages in 1952 led to this discovery • DNA provides a code, consisting of 4 letter ...
... DNA: the building blocks of genetic material • DNA was later discovered to be the molecule that makes up the inherited genetic material • Experiments performed by Fredrick Griffith in 1928 and experiments with bacteriophages in 1952 led to this discovery • DNA provides a code, consisting of 4 letter ...
14-31 - McGraw Hill Higher Education
... DNA technology in forensic science • Developed as a way of defining specific differences in DNA sequences between people – differences must be extensive and detailed enough to minimise risk of accidental identity – gene sequences are not used for this – microsatellites and minisatellites: regions o ...
... DNA technology in forensic science • Developed as a way of defining specific differences in DNA sequences between people – differences must be extensive and detailed enough to minimise risk of accidental identity – gene sequences are not used for this – microsatellites and minisatellites: regions o ...
Evolution of synonymous codon usage in metazoans Laurent Duret
... rules out the possibility that the relationship between codon bias and gene expression is due to a transcriptioncoupled mutational process [8•] and thus shows directly that synonymous codon usage is shaped by natural selection in these two invertebrates. Few experimental data on the cellular abundan ...
... rules out the possibility that the relationship between codon bias and gene expression is due to a transcriptioncoupled mutational process [8•] and thus shows directly that synonymous codon usage is shaped by natural selection in these two invertebrates. Few experimental data on the cellular abundan ...
REAL-TIME PCR
... fluorescein (from the donor probe) is directly transferred to the acceptor dye by FRET. The acceptor fluorophore emits light at a different wavelength. Subsequently the fluorescent signal can be detected and measured. This happens during the annealing phase and first part of the extension phase of t ...
... fluorescein (from the donor probe) is directly transferred to the acceptor dye by FRET. The acceptor fluorophore emits light at a different wavelength. Subsequently the fluorescent signal can be detected and measured. This happens during the annealing phase and first part of the extension phase of t ...
Evolutionary Algorithms
... • if the probability that a certain number of genes is exchanged between the parent chromosomes is not the same for all possible numbers of genes • undesired, because it causes partial solutions of different lengths to have different chances of progressing to the next generation • distributional bia ...
... • if the probability that a certain number of genes is exchanged between the parent chromosomes is not the same for all possible numbers of genes • undesired, because it causes partial solutions of different lengths to have different chances of progressing to the next generation • distributional bia ...
No Slide Title
... Multiple sequence alignment: methods Progressive methods: use a guide tree (a little like a phylogenetic tree but NOT a phylogenetic tree) to determine how to combine pairwise alignments one by one to create a multiple alignment. Making multiple alignments using trees was a very popular subject in ...
... Multiple sequence alignment: methods Progressive methods: use a guide tree (a little like a phylogenetic tree but NOT a phylogenetic tree) to determine how to combine pairwise alignments one by one to create a multiple alignment. Making multiple alignments using trees was a very popular subject in ...
Press release
... protocol and secondly also on the starting weight or volume. It also depends to a large extent on the sample material itself (leaf and seed material, animal tissue, hair, etc.). sbeadex - high-quality DNA preparations The oKtopure utilises the sbeadexTM extraction chemistry developed by LGC, which i ...
... protocol and secondly also on the starting weight or volume. It also depends to a large extent on the sample material itself (leaf and seed material, animal tissue, hair, etc.). sbeadex - high-quality DNA preparations The oKtopure utilises the sbeadexTM extraction chemistry developed by LGC, which i ...
Marine integrons containing novel integrase genes
... identities over the regions compared, respectively, were grouped into a single phylotype (Hartmann and Widmer, 2006; Elsaied et al., 2007). The gene cassette richness was determined based on grouping of cassettes that had 470.0% nucleotide identities into a single cassette type (Koenig et al., 2008) ...
... identities over the regions compared, respectively, were grouped into a single phylotype (Hartmann and Widmer, 2006; Elsaied et al., 2007). The gene cassette richness was determined based on grouping of cassettes that had 470.0% nucleotide identities into a single cassette type (Koenig et al., 2008) ...
ADP Ribosylation Factor is an Essential Protein in Saccharomyces cerevisiae and is Encoded by Two Genes.
... certain restriction sites within the coding sequences are conserved, possibly explaining the failure to identify ARF2 in the experiments in which ARFI was originally cloned (39). The predicted ARF2 protein is identical in length to the ARF1 protein and 96% identical in protein sequence, differing at ...
... certain restriction sites within the coding sequences are conserved, possibly explaining the failure to identify ARF2 in the experiments in which ARFI was originally cloned (39). The predicted ARF2 protein is identical in length to the ARF1 protein and 96% identical in protein sequence, differing at ...
Why are most organelle genomes transmitted maternally?
... (sorting-out) of genetically distinct organelles (Box 1; Fig. 1), and the virtual absence of recombination [1, 2]. Due to the different evolutionary origins and inheritance modes of the genomes of the eukaryotic cell, severe evolutionary consequences arise: (i) Nuclear and organellar genomes differ ...
... (sorting-out) of genetically distinct organelles (Box 1; Fig. 1), and the virtual absence of recombination [1, 2]. Due to the different evolutionary origins and inheritance modes of the genomes of the eukaryotic cell, severe evolutionary consequences arise: (i) Nuclear and organellar genomes differ ...
v2 PEBiosystems News 2 01/00
... ICAT is a trademark of the University of Washington, exclusively licensed to Applied Biosystems Group of Applera Corporation. Paracel is a registered trademark of Paracel Inc. The ABI PRISM 3100 and the 3100-Avant Genetic Analyzers include patented technology licensed from Hitachi, Ltd. as part of a ...
... ICAT is a trademark of the University of Washington, exclusively licensed to Applied Biosystems Group of Applera Corporation. Paracel is a registered trademark of Paracel Inc. The ABI PRISM 3100 and the 3100-Avant Genetic Analyzers include patented technology licensed from Hitachi, Ltd. as part of a ...
AnsteadSeniorHonorsThesis
... of that range is entered into Flybase in order to generate the Gbrowser displaying the genes specific to that region. Each gene is then sorted through, and genes of interest are identified by their molecular function and biological process performed. Genes associated with functions such as: DNA bind ...
... of that range is entered into Flybase in order to generate the Gbrowser displaying the genes specific to that region. Each gene is then sorted through, and genes of interest are identified by their molecular function and biological process performed. Genes associated with functions such as: DNA bind ...
Investigating the DNA-Binding Interactions of Small
... thank the Goodson Group members for their help and support. I consider the Goodson group to be family. I would also like to thank my collaborators (Prof. Wilson, Prof. Imperiali, Prof. Laine) and dissertation committee members (Prof. Chen, Prof. Kuroda, Prof. Robertson). Finally, I would like to tha ...
... thank the Goodson Group members for their help and support. I consider the Goodson group to be family. I would also like to thank my collaborators (Prof. Wilson, Prof. Imperiali, Prof. Laine) and dissertation committee members (Prof. Chen, Prof. Kuroda, Prof. Robertson). Finally, I would like to tha ...
The Rad50 Signature Motif: Essential to ATP Binding and
... manganese ions for catalysis and form a DNAbinding groove that can accommodate a doublestranded DNA end.10 In vitro, Mre11 homologs exhibit 30 to 50 exonuclease activity on DNA substrates, as well as endonuclease activity on constrained structures such as hairpin ends.11 – 16 In every organism studi ...
... manganese ions for catalysis and form a DNAbinding groove that can accommodate a doublestranded DNA end.10 In vitro, Mre11 homologs exhibit 30 to 50 exonuclease activity on DNA substrates, as well as endonuclease activity on constrained structures such as hairpin ends.11 – 16 In every organism studi ...
Raven/Johnson Biology 8e
... B. Answer b is incorrect. The DNA microarray is only sensitive to the presence or absence of an mRNA, not DNA. The correct answer is c—The pattern of gene expression C. Answer c is correct. DNA microarray technology uses fluorescent probes to examine the pattern of gene expression in various samples ...
... B. Answer b is incorrect. The DNA microarray is only sensitive to the presence or absence of an mRNA, not DNA. The correct answer is c—The pattern of gene expression C. Answer c is correct. DNA microarray technology uses fluorescent probes to examine the pattern of gene expression in various samples ...
Supplementary Information Manipulation and detection of single
... were performed for the photonic nanojets generated by the TiO2 microlenses as an example. Figure S6a−c shows the simulated electric (E) field intensity distributions of the TiO2 microlenses illuminated by a plane wave without fibre (Fig. S6a) and Gaussian beams with an aligned fibre (Fig. S6b) and a ...
... were performed for the photonic nanojets generated by the TiO2 microlenses as an example. Figure S6a−c shows the simulated electric (E) field intensity distributions of the TiO2 microlenses illuminated by a plane wave without fibre (Fig. S6a) and Gaussian beams with an aligned fibre (Fig. S6b) and a ...
ARTICLES - Weizmann Institute of Science
... high probability of starting (stably positioned nucleosomes). The resulting intrinsic nucleosome organization differs qualitatively at different genomic locations. In some cases, several mutually exclusive organizations dominate (Supplementary Fig. 10a, b); in others, a single organization dominates ...
... high probability of starting (stably positioned nucleosomes). The resulting intrinsic nucleosome organization differs qualitatively at different genomic locations. In some cases, several mutually exclusive organizations dominate (Supplementary Fig. 10a, b); in others, a single organization dominates ...
Genetic tools for manipulating Acinetobacter baumannii genome: an
... creating a true deletion. Integration plasmids can also be used for unmarked gene deletion, i.e. deletion of a gene without inserting any resistance cassette. A schematic diagram for the overall method is shown in Fig. 3. In this method, a homologous fragment carrying either the mutated or the inter ...
... creating a true deletion. Integration plasmids can also be used for unmarked gene deletion, i.e. deletion of a gene without inserting any resistance cassette. A schematic diagram for the overall method is shown in Fig. 3. In this method, a homologous fragment carrying either the mutated or the inter ...
Heuris`c)search:)FastA)and)BLAST)
... An algorithm was developed which facilitates the search for similarities between newly determined amino acid sequences and sequences already available in databases. Because of the algorithm's efficiency on many microcomputers, sensitive protein database searches may now become a routine procedure fo ...
... An algorithm was developed which facilitates the search for similarities between newly determined amino acid sequences and sequences already available in databases. Because of the algorithm's efficiency on many microcomputers, sensitive protein database searches may now become a routine procedure fo ...
Cre-Lox recombination
In the field of genetics, Cre-Lox recombination is known as a site-specific recombinase technology, and is widely used to carry out deletions, insertions, translocations and inversions at specific sites in the DNA of cells. It allows the DNA modification to be targeted to a specific cell type or be triggered by a specific external stimulus. It is implemented both in eukaryotic and prokaryotic systems.The system consists of a single enzyme, Cre recombinase, that recombines a pair of short target sequences called the Lox sequences. This system can be implemented without inserting any extra supporting proteins or sequences. The Cre enzyme and the original Lox site called the LoxP sequence are derived from bacteriophage P1.Placing Lox sequences appropriately allows genes to be activated, repressed, or exchanged for other genes. At a DNA level many types of manipulations can be carried out. The activity of the Cre enzyme can be controlled so that it is expressed in a particular cell type or triggered by an external stimulus like a chemical signal or a heat shock. These targeted DNA changes are useful in cell lineage tracing and when mutants are lethal if expressed globally.The Cre-Lox system is very similar in action and in usage to the FLP-FRT recombination system.