Identifikasi Molekular Bakteri Pathogen yang Menginfeksi Hama
... (Ishii et al., 2014b). Cecropins are used by insect as the main defence against gram-negative bacteria. Three different proteases exist in S. marcescens can destroy cecropins (Fly et al., 1983). It has been proposed that outer membrane vesicles (OMVs) produced by bacteria might act as long-range tox ...
... (Ishii et al., 2014b). Cecropins are used by insect as the main defence against gram-negative bacteria. Three different proteases exist in S. marcescens can destroy cecropins (Fly et al., 1983). It has been proposed that outer membrane vesicles (OMVs) produced by bacteria might act as long-range tox ...
Gene Detection Systems Catalog
... Gene Link, Inc. is a dynamic biotechnology company and research organization. Our mission is to be one of the most reliable suppliers of reagents and reagent systems used in genetic research technology applications worldwide. Gene Link, Inc. is privately held biotechnology company incorporated in th ...
... Gene Link, Inc. is a dynamic biotechnology company and research organization. Our mission is to be one of the most reliable suppliers of reagents and reagent systems used in genetic research technology applications worldwide. Gene Link, Inc. is privately held biotechnology company incorporated in th ...
final1-eu-marie-curie-final-report
... 3% of the genes were dedicated to secondary metabolite production, biosynthesis, transport and catabolism.3 Questions therefore arise as to whether or not the natural products are strain specific, especially as most taxonomic classifications have been mostly morphological. It is also clear through t ...
... 3% of the genes were dedicated to secondary metabolite production, biosynthesis, transport and catabolism.3 Questions therefore arise as to whether or not the natural products are strain specific, especially as most taxonomic classifications have been mostly morphological. It is also clear through t ...
Transcription
... metabolic activities. In present day cells, DNA stores information and proteins perform catalysis, with RNA as the intermediate between DNA and protein. One can imagine a time when there was no DNA or protein, just RNA performing both functions: this is the RNA World hypothesis. • Very long ago, at ...
... metabolic activities. In present day cells, DNA stores information and proteins perform catalysis, with RNA as the intermediate between DNA and protein. One can imagine a time when there was no DNA or protein, just RNA performing both functions: this is the RNA World hypothesis. • Very long ago, at ...
Transcription
... • It is necessary to unwind the DNA so that the antisense strand to become accessible for base pairing and RNA synthesis. • Negative supercoiling enhances the transcription of many genes, since it facilitates unwinding. Some promoters are not. • Exceptional example: promters for the enzyme subunits ...
... • It is necessary to unwind the DNA so that the antisense strand to become accessible for base pairing and RNA synthesis. • Negative supercoiling enhances the transcription of many genes, since it facilitates unwinding. Some promoters are not. • Exceptional example: promters for the enzyme subunits ...
DNA Evolution 3.1 Troubleshooting and Debugging Guide
... © 2013 StorageDNA, Inc. All rights reserved. The owner or authorized user of a valid copy of DNA Evolution may reproduce this publication for the purpose of learning to use such software. No part of this publication may be reproduced or transmitted for commercial purposes, such as ...
... © 2013 StorageDNA, Inc. All rights reserved. The owner or authorized user of a valid copy of DNA Evolution may reproduce this publication for the purpose of learning to use such software. No part of this publication may be reproduced or transmitted for commercial purposes, such as ...
molecular phylogeny of the haplosporidia based on
... The SSU rRNA genes for most of the haplosporidian species were amplified in the polymerase chain reaction (PCR) using ‘‘universal’’ eukaryotic primers (Medlin et al., 1988) and conditions previously described (Flores et al., 1996). Internal SSU rRNA gene primers Urocomp280SSU (Reece and Stokes, 2003 ...
... The SSU rRNA genes for most of the haplosporidian species were amplified in the polymerase chain reaction (PCR) using ‘‘universal’’ eukaryotic primers (Medlin et al., 1988) and conditions previously described (Flores et al., 1996). Internal SSU rRNA gene primers Urocomp280SSU (Reece and Stokes, 2003 ...
Killing specimens
... they are difficult to insert through labels and into pinning surfaces, and often result in abdomens (or other body parts) getting “flinged” across the room (due to their lack of stiffness). If specimens require these smaller size pins consider double-mounting. 7. Double mounting: Double mounting inc ...
... they are difficult to insert through labels and into pinning surfaces, and often result in abdomens (or other body parts) getting “flinged” across the room (due to their lack of stiffness). If specimens require these smaller size pins consider double-mounting. 7. Double mounting: Double mounting inc ...
Solid Tumour Section Esophagus: Barrett's esophagus, dysplasia and adenocarcinoma
... weight loss, pain in the throat or mid-chest, etc. ...
... weight loss, pain in the throat or mid-chest, etc. ...
Role of base-backbone and base-base interactions
... helical twist than the average (Table 1). In contrast, the YR steps in B-DNA and in the protein complexes are characterised with high standard deviations in roll, helical twist, and slide parameters, and thus these are more flexible than the others. The slide parameter at YR steps is positive on the ...
... helical twist than the average (Table 1). In contrast, the YR steps in B-DNA and in the protein complexes are characterised with high standard deviations in roll, helical twist, and slide parameters, and thus these are more flexible than the others. The slide parameter at YR steps is positive on the ...
III BSC BT - 609 A -core- Animal Biotechnology
... A. it contains aggression genes. B. it contains multiple copies of a human gene. C. it contains an activated human gene. D. none of the above. ANSWER: C 112. The first patent for a transgenic organism was awarded in 1988. Which organism was patented? A. A yeast used in industrial processes. B. A bac ...
... A. it contains aggression genes. B. it contains multiple copies of a human gene. C. it contains an activated human gene. D. none of the above. ANSWER: C 112. The first patent for a transgenic organism was awarded in 1988. Which organism was patented? A. A yeast used in industrial processes. B. A bac ...
Author`s personal copy
... If a highly efficient method for mutant construction is developed, a genome-wide analysis of gene function is possible (Hammelmann and Soppa, 2008). It would not only accelerate the genome-wide identification of the genes responsible for PHA biosynthesis, but may also be used to genetically engineer ...
... If a highly efficient method for mutant construction is developed, a genome-wide analysis of gene function is possible (Hammelmann and Soppa, 2008). It would not only accelerate the genome-wide identification of the genes responsible for PHA biosynthesis, but may also be used to genetically engineer ...
Translational Repression of C. elegans p53 by GLD
... 2003; Vassilev et al., 2004). Such a therapeutic approach highlights the need to uncover additional pathways and mechanisms that negatively regulate p53 levels or activity. Most studies on p53 signaling have been conducted in cell culture-based systems, and their translation into mouse models is oft ...
... 2003; Vassilev et al., 2004). Such a therapeutic approach highlights the need to uncover additional pathways and mechanisms that negatively regulate p53 levels or activity. Most studies on p53 signaling have been conducted in cell culture-based systems, and their translation into mouse models is oft ...
BIO301 - National Open University of Nigeria
... depending on their reproductive success, the changes due to genetic drift are not driven by environmental or adaptive pressures, and may be beneficial, neutral, or detrimental to reproductive success. The effect of genetic drift is larger for alleles present in a smaller number of copies, and smalle ...
... depending on their reproductive success, the changes due to genetic drift are not driven by environmental or adaptive pressures, and may be beneficial, neutral, or detrimental to reproductive success. The effect of genetic drift is larger for alleles present in a smaller number of copies, and smalle ...
Real time PCR based determination of gene copy numbers in
... strain analysis. 2 ng of genomic DNA were added in a reaction volume of 20 µL. For each unknown strain, reactions with GFP and ARG4 primer sets were performed in separate tubes. Standard curves and NTCs for both genes were recorded in every plate. The reactions were done in MicroAmp® Optical 96-Well ...
... strain analysis. 2 ng of genomic DNA were added in a reaction volume of 20 µL. For each unknown strain, reactions with GFP and ARG4 primer sets were performed in separate tubes. Standard curves and NTCs for both genes were recorded in every plate. The reactions were done in MicroAmp® Optical 96-Well ...
fragile x dna testing: a guide for physicians and families
... of choice for identifying full mutations and large premutations and determining if the gene is methylated while PCR analysis allows accurate determination of CGG repeat number for normal, grey zone and premutation alleles. Fragile X testing is most accurate and reliable when both approaches are used ...
... of choice for identifying full mutations and large premutations and determining if the gene is methylated while PCR analysis allows accurate determination of CGG repeat number for normal, grey zone and premutation alleles. Fragile X testing is most accurate and reliable when both approaches are used ...
Spider Silk - Consortium for Mathematics and its Applications
... Because of significant laboratory research, we already know the amino acid sequences for many silk proteins. Additionally, research suggests that the technology to manufacture spider silk is not too far off. But perhaps we could do even better. What if we changed the amino acid sequence? Could we fi ...
... Because of significant laboratory research, we already know the amino acid sequences for many silk proteins. Additionally, research suggests that the technology to manufacture spider silk is not too far off. But perhaps we could do even better. What if we changed the amino acid sequence? Could we fi ...
High-Level Expression in Escherichia coli of Alkaline Phosphatase
... stable at temperatures above 80oC, activated by Mg2+, and inhibited by Zn2+. In contrast, Zn2+ is essential for the activity of E. coli APase [1, 2, 7]. The gene encoding Tca APase was recently cloned into E. coli and the primary structure was deduced from its nucleotide sequence [9]. Like E. coli e ...
... stable at temperatures above 80oC, activated by Mg2+, and inhibited by Zn2+. In contrast, Zn2+ is essential for the activity of E. coli APase [1, 2, 7]. The gene encoding Tca APase was recently cloned into E. coli and the primary structure was deduced from its nucleotide sequence [9]. Like E. coli e ...
Transgenic Plastids in Basic Research and Plant Biotechnology
... transformation event involves the change of only a single (or at most a few) out of several thousand plastid genome copies in a leaf cell. During subsequent cell and organelle divisions, the presence of high concentrations of the selecting antibiotic favors multiplication of chloroplasts containing ...
... transformation event involves the change of only a single (or at most a few) out of several thousand plastid genome copies in a leaf cell. During subsequent cell and organelle divisions, the presence of high concentrations of the selecting antibiotic favors multiplication of chloroplasts containing ...
PPT - Altogen Biosystems
... 5. Optional: Add 2 µl of Complex Condenser. This reagent reduces the size of transfection complex, therefore increasing transfection efficiency; however it may increase cell toxicity 6. Add prepared transfection complexes to 0.5 ml of complete growth medium with Beta-TC-6 cells (from step 2) 7. Incu ...
... 5. Optional: Add 2 µl of Complex Condenser. This reagent reduces the size of transfection complex, therefore increasing transfection efficiency; however it may increase cell toxicity 6. Add prepared transfection complexes to 0.5 ml of complete growth medium with Beta-TC-6 cells (from step 2) 7. Incu ...
DNA Evidence: Probability, Population Genetics, and the Courts
... 7. See David H. Kaye, The Admissibility ofDNA Testing, 13 CARDOZO L. REV. 353, 357 n.17 (1991). A case that is representative of this epoch is Cobey v. State, 559 A.2d 391 (Md. Ct. Spec. App. 1988). A man forced a woman jogging in a park into the woods, where, as the court of appeals put it, he "rav ...
... 7. See David H. Kaye, The Admissibility ofDNA Testing, 13 CARDOZO L. REV. 353, 357 n.17 (1991). A case that is representative of this epoch is Cobey v. State, 559 A.2d 391 (Md. Ct. Spec. App. 1988). A man forced a woman jogging in a park into the woods, where, as the court of appeals put it, he "rav ...
WS Procs 975 x 65
... Weeder [19], MEME [13] and SPSP-Finder [7], with DSP-Finder on the yeast data set in SCPD [25]. SCPD contains information of the motif patterns and the binding sites for a set of transcription factors of yeast. For each transcription factor, we chose the 600 base pairs in the upstream of those genes ...
... Weeder [19], MEME [13] and SPSP-Finder [7], with DSP-Finder on the yeast data set in SCPD [25]. SCPD contains information of the motif patterns and the binding sites for a set of transcription factors of yeast. For each transcription factor, we chose the 600 base pairs in the upstream of those genes ...
Cre-Lox recombination
In the field of genetics, Cre-Lox recombination is known as a site-specific recombinase technology, and is widely used to carry out deletions, insertions, translocations and inversions at specific sites in the DNA of cells. It allows the DNA modification to be targeted to a specific cell type or be triggered by a specific external stimulus. It is implemented both in eukaryotic and prokaryotic systems.The system consists of a single enzyme, Cre recombinase, that recombines a pair of short target sequences called the Lox sequences. This system can be implemented without inserting any extra supporting proteins or sequences. The Cre enzyme and the original Lox site called the LoxP sequence are derived from bacteriophage P1.Placing Lox sequences appropriately allows genes to be activated, repressed, or exchanged for other genes. At a DNA level many types of manipulations can be carried out. The activity of the Cre enzyme can be controlled so that it is expressed in a particular cell type or triggered by an external stimulus like a chemical signal or a heat shock. These targeted DNA changes are useful in cell lineage tracing and when mutants are lethal if expressed globally.The Cre-Lox system is very similar in action and in usage to the FLP-FRT recombination system.