Eds., N. Hamamura, S. Suzuki, S. Mendo, C. M. Barroso,... © by TERRAPUB, 2010.
... coli HB101 cells, and growth experiments in liquid media were performed with each of the sub clone in LB medium containing ampicillin and 100 µM TBT. Only sub clones P2, P3, P4 and P6 of clone 69, were resistant to TBT (Fig. 1). Growth was compared to that of the clone 69 and also to the negative co ...
... coli HB101 cells, and growth experiments in liquid media were performed with each of the sub clone in LB medium containing ampicillin and 100 µM TBT. Only sub clones P2, P3, P4 and P6 of clone 69, were resistant to TBT (Fig. 1). Growth was compared to that of the clone 69 and also to the negative co ...
Alu Human Polymorphism
... • What are the consequences and benefits of having an Alu element? • Alu inserting into exons has been implicated in neurofibromatosis, cancer and heart attacks • They can provide alternate splicing in 5% of genes which leads to genetic diversity • Can provide information on migration and evolution ...
... • What are the consequences and benefits of having an Alu element? • Alu inserting into exons has been implicated in neurofibromatosis, cancer and heart attacks • They can provide alternate splicing in 5% of genes which leads to genetic diversity • Can provide information on migration and evolution ...
The Parasexual Cycle in Candida albicans Provides an
... Candida albicans has an elaborate, yet efficient, mating system that promotes conjugation between diploid a and a strains. The product of mating is a tetraploid a/a cell that must undergo a reductional division to return to the diploid state. Despite the presence of several ‘‘meiosis-specific’’ gene ...
... Candida albicans has an elaborate, yet efficient, mating system that promotes conjugation between diploid a and a strains. The product of mating is a tetraploid a/a cell that must undergo a reductional division to return to the diploid state. Despite the presence of several ‘‘meiosis-specific’’ gene ...
Curr. Microbiol. 42
... by their ability to produce during sporulation large quantities of insect larvicidal proteins (␦-endotoxins) aggregated in parasporal crystalline bodies [e.g., 6, 20]. The high potencies and specificities of B. thuringiensis insecticidal crystal (Cry) proteins have spurred their use as natural pest ...
... by their ability to produce during sporulation large quantities of insect larvicidal proteins (␦-endotoxins) aggregated in parasporal crystalline bodies [e.g., 6, 20]. The high potencies and specificities of B. thuringiensis insecticidal crystal (Cry) proteins have spurred their use as natural pest ...
Forche et al. 2008 PLoS Biology
... Candida albicans has an elaborate, yet efficient, mating system that promotes conjugation between diploid a and a strains. The product of mating is a tetraploid a/a cell that must undergo a reductional division to return to the diploid state. Despite the presence of several ‘‘meiosis-specific’’ gene ...
... Candida albicans has an elaborate, yet efficient, mating system that promotes conjugation between diploid a and a strains. The product of mating is a tetraploid a/a cell that must undergo a reductional division to return to the diploid state. Despite the presence of several ‘‘meiosis-specific’’ gene ...
- Philsci-Archive
... My strategy in what follows will be to introduce a very undemanding or minimalist notion of causation, based on the interventionist framework described in Woodward, 2003. I will then use this as a basis on which to explore the various other distinctions, having to do with stability, specificity and ...
... My strategy in what follows will be to introduce a very undemanding or minimalist notion of causation, based on the interventionist framework described in Woodward, 2003. I will then use this as a basis on which to explore the various other distinctions, having to do with stability, specificity and ...
Myriad--Ambry -- Final Version of Ambry Preliminary Injunction
... Chromosomes in a human cell are made up of two complementary strands of DNA molecules—one strand is on one side of the double helix and the second strand is on the other side. For any given gene, only one molecule strand (the “template strand”) is actually transcribed into mRNA and ultimately used t ...
... Chromosomes in a human cell are made up of two complementary strands of DNA molecules—one strand is on one side of the double helix and the second strand is on the other side. For any given gene, only one molecule strand (the “template strand”) is actually transcribed into mRNA and ultimately used t ...
A Novel CpG Island Set Identifies Tissue-Specific
... 3C). The results established that M values greater than 1.5 denote CGIs that are significantly enriched by MAP and therefore methylated. CGIs of the BEST1 and R4RL1 genes were predicted to be nonmethylated (M ¼ 0.2–0.4) and methylated (M ¼ 2.2–2.8), respectively, based on the array data. Bisulfite gen ...
... 3C). The results established that M values greater than 1.5 denote CGIs that are significantly enriched by MAP and therefore methylated. CGIs of the BEST1 and R4RL1 genes were predicted to be nonmethylated (M ¼ 0.2–0.4) and methylated (M ¼ 2.2–2.8), respectively, based on the array data. Bisulfite gen ...
Systematic and Applied Microbiology - digital
... analyzed, produced tyramine. However, in both cases the production of biogenic amines was only evaluated in a decarboxylase agar medium where, sometimes, false-positive reactions have been described [10]. The results of our study demonstrate that the E. faecium strains analyzed actually possess a ge ...
... analyzed, produced tyramine. However, in both cases the production of biogenic amines was only evaluated in a decarboxylase agar medium where, sometimes, false-positive reactions have been described [10]. The results of our study demonstrate that the E. faecium strains analyzed actually possess a ge ...
M.Sc., Biotechnology - Alagappa University
... Prior-learning will help the students to understand better about the topic taken in the class. All the students are anticipated to receive a strong background and be able to participate in group discussions of the assigned topics prior to attending the talk. The students are intimated about the topi ...
... Prior-learning will help the students to understand better about the topic taken in the class. All the students are anticipated to receive a strong background and be able to participate in group discussions of the assigned topics prior to attending the talk. The students are intimated about the topi ...
Physical Mapping of a 670-kb Region of Chromosomes XVI and XVII
... blocks containing whole chromosomal T. cruzi DNA were incubated with increasing amounts of exonuclease Bal31 following digestion with SfiI restriction enzyme (Fig. 3). The loci h49 were located in two SfiI fragments of 350 kb and 450 kb that were sensitive to the treatment with Bal31. On the other h ...
... blocks containing whole chromosomal T. cruzi DNA were incubated with increasing amounts of exonuclease Bal31 following digestion with SfiI restriction enzyme (Fig. 3). The loci h49 were located in two SfiI fragments of 350 kb and 450 kb that were sensitive to the treatment with Bal31. On the other h ...
2.2 Australian Pilot Survey for GM Food Labelling
... it is no longer recognised or detected by analysis [7]. For highly processed products, such as sugar and oils, the production process removes the proteins and DNA ...
... it is no longer recognised or detected by analysis [7]. For highly processed products, such as sugar and oils, the production process removes the proteins and DNA ...
Nucleic Acids - Farmasi Unand
... • In RNA only intrastrand cross-links are possible. However, irrespective of whether or not it forms a bridge. the bonding of an alkylating agent to a nucleic acid inhibits replication of that nucleic acid prof. aza ...
... • In RNA only intrastrand cross-links are possible. However, irrespective of whether or not it forms a bridge. the bonding of an alkylating agent to a nucleic acid inhibits replication of that nucleic acid prof. aza ...
Characterisation of interstitial duplications and triplications of
... showed that, in all cases, the duplications and triplications involved the PWACR and were not pseudogene expansions. Retrospective cytogenetic analysis in families 7 and 13 did not identify these duplications clearly. The size of the pericentromeric area of 15q varies greatly within the normal popul ...
... showed that, in all cases, the duplications and triplications involved the PWACR and were not pseudogene expansions. Retrospective cytogenetic analysis in families 7 and 13 did not identify these duplications clearly. The size of the pericentromeric area of 15q varies greatly within the normal popul ...
Analysis of acid-induced asr gene promoter of Enterobacteriaceae
... Deletion of the distal region upstream –70 did not influence the acid-induced activity of asr promoter (Fig. 1, lane 6 vs. 4), indicating that it contains no elements of asr transcription control. However, deletions close to the –60 position significantly decreased the activity of asr promoter (Fig. ...
... Deletion of the distal region upstream –70 did not influence the acid-induced activity of asr promoter (Fig. 1, lane 6 vs. 4), indicating that it contains no elements of asr transcription control. However, deletions close to the –60 position significantly decreased the activity of asr promoter (Fig. ...
Lab: Colony PCR amplification of the 16S ribosomal RNA gene I
... 3. Seal the ends of the gel form to prepare it for pouring. In some cases, the gel forms are sealed by their gasketed ends in the gel bed; in others, they have an external device for sealing the ends of the form. It is unique for each gel setup, so if you are unsure ask for assistance. Once the gel ...
... 3. Seal the ends of the gel form to prepare it for pouring. In some cases, the gel forms are sealed by their gasketed ends in the gel bed; in others, they have an external device for sealing the ends of the form. It is unique for each gel setup, so if you are unsure ask for assistance. Once the gel ...
My PP Genetics
... By 1950, scientists knew DNA was the genetic material, but they did not know the structure of DNA. In 1953, Watson and Crick built a model of DNA that was consistent with available evidence. They used X-ray photos of DNA taken by Franklin and Wilkins as part of their research. ...
... By 1950, scientists knew DNA was the genetic material, but they did not know the structure of DNA. In 1953, Watson and Crick built a model of DNA that was consistent with available evidence. They used X-ray photos of DNA taken by Franklin and Wilkins as part of their research. ...
An rpoB signature sequence provides unique resolution for the
... groups possible in addition to improving strain assignment. Conserved indels (insertions/ deletions) are present in all cyanobacterial RpoB (b subunit of RNA polymerase) sequences presently available in public databases. These indels are located in the Rpb2_6 domain of RpoB, which is involved in DNA ...
... groups possible in addition to improving strain assignment. Conserved indels (insertions/ deletions) are present in all cyanobacterial RpoB (b subunit of RNA polymerase) sequences presently available in public databases. These indels are located in the Rpb2_6 domain of RpoB, which is involved in DNA ...
Capstone project 2015 in pdf format
... they are also devoid of detectable-nucleosomes and their chromosomes are apparently quasi-condensed throughout the cell cycle. Why do they have so much DNA is still a mystery; one hypothesis of non-coding domains being “structural” support to the coding domains ...
... they are also devoid of detectable-nucleosomes and their chromosomes are apparently quasi-condensed throughout the cell cycle. Why do they have so much DNA is still a mystery; one hypothesis of non-coding domains being “structural” support to the coding domains ...
Molecular Diagnostics in Clinical Microbiology
... sensitivity and specificity of MRSA testing. Below, the molecular targets used for MRSA detection will be defined and the test systems that are currently available will be described. Clinically relevant meticillin resistance in S. aureus is the result of the acquisition of an alternative penicillin ...
... sensitivity and specificity of MRSA testing. Below, the molecular targets used for MRSA detection will be defined and the test systems that are currently available will be described. Clinically relevant meticillin resistance in S. aureus is the result of the acquisition of an alternative penicillin ...
Two groups of human herpesvirus 6 identified by sequence
... fulminant hepatitis (Asano et al., 1990) and HHV-6associated interstitial pneumonitis in bone marrow transplant (BMT) patients (Carrigan et al., 1991). Furthermore, a marrow-suppressive role for HHV-6 has been proposed (Knox & Carrigan, 1992; Drobyski et al., 1992). There has been conflicting eviden ...
... fulminant hepatitis (Asano et al., 1990) and HHV-6associated interstitial pneumonitis in bone marrow transplant (BMT) patients (Carrigan et al., 1991). Furthermore, a marrow-suppressive role for HHV-6 has been proposed (Knox & Carrigan, 1992; Drobyski et al., 1992). There has been conflicting eviden ...
Cre-Lox recombination
In the field of genetics, Cre-Lox recombination is known as a site-specific recombinase technology, and is widely used to carry out deletions, insertions, translocations and inversions at specific sites in the DNA of cells. It allows the DNA modification to be targeted to a specific cell type or be triggered by a specific external stimulus. It is implemented both in eukaryotic and prokaryotic systems.The system consists of a single enzyme, Cre recombinase, that recombines a pair of short target sequences called the Lox sequences. This system can be implemented without inserting any extra supporting proteins or sequences. The Cre enzyme and the original Lox site called the LoxP sequence are derived from bacteriophage P1.Placing Lox sequences appropriately allows genes to be activated, repressed, or exchanged for other genes. At a DNA level many types of manipulations can be carried out. The activity of the Cre enzyme can be controlled so that it is expressed in a particular cell type or triggered by an external stimulus like a chemical signal or a heat shock. These targeted DNA changes are useful in cell lineage tracing and when mutants are lethal if expressed globally.The Cre-Lox system is very similar in action and in usage to the FLP-FRT recombination system.