Evidence that MEK1 positively promotes
... invasion. Using two different reporter cassettes that contain the recognition sequence for the VMA1-derived homing endonuclease (VDE), we have previously reported on various factors that influence DSB repair during meiosis (22,23). Here we make use of the VDE-induced DSBs (VDE–DSBs) to remove the like ...
... invasion. Using two different reporter cassettes that contain the recognition sequence for the VMA1-derived homing endonuclease (VDE), we have previously reported on various factors that influence DSB repair during meiosis (22,23). Here we make use of the VDE-induced DSBs (VDE–DSBs) to remove the like ...
(From the Department of Genetics, Carnegie Institution of
... Glycerol-lactate medium was chosen to permit growth of bacteria without undesirable pH changes at low concentrations of phosphorus and sulfur, and proved useful also for certain experiments described in this paper. 18-hour cultures of sensitive bacteria grown in this medium contain about 2 × 109 cel ...
... Glycerol-lactate medium was chosen to permit growth of bacteria without undesirable pH changes at low concentrations of phosphorus and sulfur, and proved useful also for certain experiments described in this paper. 18-hour cultures of sensitive bacteria grown in this medium contain about 2 × 109 cel ...
Independent functions of viral protein and nucleic
... that shown in Table III, and the content of Pa was only slightly less after lysis. The phage liberated during freezing and thawing was also fitrated in this experiment. The lysis occurred without appreciable liberation of phage in suspensions frozen up to and including the 16th minute, and the 20 mi ...
... that shown in Table III, and the content of Pa was only slightly less after lysis. The phage liberated during freezing and thawing was also fitrated in this experiment. The lysis occurred without appreciable liberation of phage in suspensions frozen up to and including the 16th minute, and the 20 mi ...
Chapter 3 Transposon Mutagenesis of Rhodobacter sphaeroides
... Transposon mutagenesis has several advantages over the use of other methods of mutation. First, chemicals and ionizing radiation not only can mutagenize the intended microorganism, they can also mutagenize you! Extreme caution must be exercised when employing these techniques. Transposons that mutag ...
... Transposon mutagenesis has several advantages over the use of other methods of mutation. First, chemicals and ionizing radiation not only can mutagenize the intended microorganism, they can also mutagenize you! Extreme caution must be exercised when employing these techniques. Transposons that mutag ...
Expression and characterization of 1
... spectrometry to an accuracy of F0.02% at the Pasarow Mass Spectrometry Laboratory at UCLA. 2.4. Enzyme assays UV–Vis spectra and steady-state kinetics were recorded on a Shimadzu UV-2501 double-beam spectrophotometer equipped with a thermostat cell holder. Enzyme activity was measured by a coupled a ...
... spectrometry to an accuracy of F0.02% at the Pasarow Mass Spectrometry Laboratory at UCLA. 2.4. Enzyme assays UV–Vis spectra and steady-state kinetics were recorded on a Shimadzu UV-2501 double-beam spectrophotometer equipped with a thermostat cell holder. Enzyme activity was measured by a coupled a ...
pdf
... separating the two strands (Fig. 3.1.10). The two strands are rewound at another active site, regenerating duplex DNA. c. Within the unwound region (bubble), the 3' terminus of the growing RNA chain is bound to its complement on the template strand via H-bonding. The DNA strand whose sequence is the ...
... separating the two strands (Fig. 3.1.10). The two strands are rewound at another active site, regenerating duplex DNA. c. Within the unwound region (bubble), the 3' terminus of the growing RNA chain is bound to its complement on the template strand via H-bonding. The DNA strand whose sequence is the ...
hag expression in Bacillus subtilis is both negatively
... (Caldwell et al., 2001), which are mainly transcribed by the sD-dependent RNA polymerase (Ordal et al., 1993). This conclusion has been derived from the transcription profiling of scoC mutant cells (scoC4), which showed that most of the motility genes, including hag, are transcribed at lower levels ...
... (Caldwell et al., 2001), which are mainly transcribed by the sD-dependent RNA polymerase (Ordal et al., 1993). This conclusion has been derived from the transcription profiling of scoC mutant cells (scoC4), which showed that most of the motility genes, including hag, are transcribed at lower levels ...
Tandem Genetic Duplications in Phage and Bacteria
... Within the last several years, methods have been developed for detection and analysis of tandem duplications of genetic material in bacteria and phage. Although the mechanisms for formation of these duplications are not yet completely clear, it seems reasonable to review the accumulating literature ...
... Within the last several years, methods have been developed for detection and analysis of tandem duplications of genetic material in bacteria and phage. Although the mechanisms for formation of these duplications are not yet completely clear, it seems reasonable to review the accumulating literature ...
Slides: background and project plan
... • Each cell contains an identical copy of the whole genome - but utilizes only a subset of the genes to perform diverse, unique tasks • Most genes are highly regulated – their expression is limited to specific tissues, developmental stages, physiological condition • Main regulatory mechanism – trans ...
... • Each cell contains an identical copy of the whole genome - but utilizes only a subset of the genes to perform diverse, unique tasks • Most genes are highly regulated – their expression is limited to specific tissues, developmental stages, physiological condition • Main regulatory mechanism – trans ...
UCSD_PevznerMolecula.. - Purdue University :: Computer Science
... • basic physical and functional units of heredity. • specific sequences of DNA bases that encode instructions on how to make proteins. • Proteins ...
... • basic physical and functional units of heredity. • specific sequences of DNA bases that encode instructions on how to make proteins. • Proteins ...
Primer on Molecular Biology
... • basic physical and functional units of heredity. • specific sequences of DNA bases that encode instructions on how to make proteins. • Proteins ...
... • basic physical and functional units of heredity. • specific sequences of DNA bases that encode instructions on how to make proteins. • Proteins ...
Primer on Molecular Biology
... • basic physical and functional units of heredity. • specific sequences of DNA bases that encode instructions on how to make proteins. • Proteins ...
... • basic physical and functional units of heredity. • specific sequences of DNA bases that encode instructions on how to make proteins. • Proteins ...
DNA
... • basic physical and functional units of heredity. • specific sequences of DNA bases that encode instructions on how to make proteins. • Proteins ...
... • basic physical and functional units of heredity. • specific sequences of DNA bases that encode instructions on how to make proteins. • Proteins ...
a nine-base pair deletion distinguishes two en/spm
... known since no detectable change occurred with the element (though the complete sequence of 8000+ bases had not been determined). Nevertheless, if enough exceptions were pursued via molecular analysis, a number of alterations might be anticipated in the vicinity of insertion sites. Whether there is ...
... known since no detectable change occurred with the element (though the complete sequence of 8000+ bases had not been determined). Nevertheless, if enough exceptions were pursued via molecular analysis, a number of alterations might be anticipated in the vicinity of insertion sites. Whether there is ...
Chapter 4
... coding sequence which length is not divisible by three. In addition, we can annotate protein sequences formed by an inversion and shifted variants of inversions as well. A frameshift variant is a genetic mutation due to insertions or deletions on a DNA sequence that is consequently translated into a ...
... coding sequence which length is not divisible by three. In addition, we can annotate protein sequences formed by an inversion and shifted variants of inversions as well. A frameshift variant is a genetic mutation due to insertions or deletions on a DNA sequence that is consequently translated into a ...
Identification of New Genes Involved in Meiosis by a Genetic Screen
... events on the DNA level including formation of DNA double strand breaks (DSBs) and processing them into cross over (CO) product on one hand, and assembly and disassembly of synaptonemal complex (discussed below) on the other, occur, resulting in chromosome condensation. During metaphase I, homologou ...
... events on the DNA level including formation of DNA double strand breaks (DSBs) and processing them into cross over (CO) product on one hand, and assembly and disassembly of synaptonemal complex (discussed below) on the other, occur, resulting in chromosome condensation. During metaphase I, homologou ...
htp Pseudomonas aeruginosa AMANDA SWANEPOEL
... opportunistic pathogen of humans, causing infections in immunocompromised patients such as those with cancer or AIDS and those suffering from cystic fibrosis and burns (Van Delden and Iglewski, 1998; Ramsey and Wozniak, 2005), but it has also been shown to infect plants and insects (Schroth et al., ...
... opportunistic pathogen of humans, causing infections in immunocompromised patients such as those with cancer or AIDS and those suffering from cystic fibrosis and burns (Van Delden and Iglewski, 1998; Ramsey and Wozniak, 2005), but it has also been shown to infect plants and insects (Schroth et al., ...
Application of whole genome sequencing to fully characterise
... sequence (of strain NCTC11168) was published in 2000 (28), numerous other Campylobacter genomes have been sequenced, revealing extensive within-species diversity (29-31). Building on these comparative studies involving relatively small numbers of isolates, WGS analysis has since been used in genome- ...
... sequence (of strain NCTC11168) was published in 2000 (28), numerous other Campylobacter genomes have been sequenced, revealing extensive within-species diversity (29-31). Building on these comparative studies involving relatively small numbers of isolates, WGS analysis has since been used in genome- ...
A Cladistic Analysis of Phenotype Associations with
... taining genes with known biochemical functions. By simultaneously studying traits that are related to the genes’ known biochemical or physiological functions, it is possible to assign phenotypic effects to specific alleles or haplotypes at or near the genes of knownfunction. This measured genotype a ...
... taining genes with known biochemical functions. By simultaneously studying traits that are related to the genes’ known biochemical or physiological functions, it is possible to assign phenotypic effects to specific alleles or haplotypes at or near the genes of knownfunction. This measured genotype a ...
Rh phenotype prediction by DNA typing and its
... 1997). No consensus has yet been achieved as to which polymorphism may be most reliable for testing of any given population. RH and particularly RHD DNA typing is much confounded by the presence of two highly homologous genes, RHCE and RHD, and the complex polymorphisms between both genes. In contra ...
... 1997). No consensus has yet been achieved as to which polymorphism may be most reliable for testing of any given population. RH and particularly RHD DNA typing is much confounded by the presence of two highly homologous genes, RHCE and RHD, and the complex polymorphisms between both genes. In contra ...
Getting Started With Gel Electrophoresis
... The gel used in electrophoresis is made of a solution of agarose, a polysaccharide obtained from red algae. When dissolved in hot water and allowed to cool, the solution turns into a gel because long agarose molecules hydrogen bond together, creating a large, threedimensional network trapping the wa ...
... The gel used in electrophoresis is made of a solution of agarose, a polysaccharide obtained from red algae. When dissolved in hot water and allowed to cool, the solution turns into a gel because long agarose molecules hydrogen bond together, creating a large, threedimensional network trapping the wa ...
Primers BSHG06 - National Genetics Reference Laboratories
... Conclusions and future work We have developed a prototype specification for validated primer design and optimisation and have demonstrated its utility in the context of a fully automated sample processing system. We have also implemented an automated optimisation pipeline that will expedite the ...
... Conclusions and future work We have developed a prototype specification for validated primer design and optimisation and have demonstrated its utility in the context of a fully automated sample processing system. We have also implemented an automated optimisation pipeline that will expedite the ...
Molecular Diagnostics for the Detection and Characterization of
... of the infectious agent so that a narrower-spectrum antimicrobial agent may be used, which should have an impact on resistance patterns. It has been 12 decades since the inception of the PCR [1, 2]. Although this technique was almost immediately implemented in research laboratories to study a variet ...
... of the infectious agent so that a narrower-spectrum antimicrobial agent may be used, which should have an impact on resistance patterns. It has been 12 decades since the inception of the PCR [1, 2]. Although this technique was almost immediately implemented in research laboratories to study a variet ...
Cre-Lox recombination
In the field of genetics, Cre-Lox recombination is known as a site-specific recombinase technology, and is widely used to carry out deletions, insertions, translocations and inversions at specific sites in the DNA of cells. It allows the DNA modification to be targeted to a specific cell type or be triggered by a specific external stimulus. It is implemented both in eukaryotic and prokaryotic systems.The system consists of a single enzyme, Cre recombinase, that recombines a pair of short target sequences called the Lox sequences. This system can be implemented without inserting any extra supporting proteins or sequences. The Cre enzyme and the original Lox site called the LoxP sequence are derived from bacteriophage P1.Placing Lox sequences appropriately allows genes to be activated, repressed, or exchanged for other genes. At a DNA level many types of manipulations can be carried out. The activity of the Cre enzyme can be controlled so that it is expressed in a particular cell type or triggered by an external stimulus like a chemical signal or a heat shock. These targeted DNA changes are useful in cell lineage tracing and when mutants are lethal if expressed globally.The Cre-Lox system is very similar in action and in usage to the FLP-FRT recombination system.