Pdf - Text of NPTEL IIT Video Lectures
... The content of parental D N A in double helix decreases with each generation according to the semi-conservative model of replication. Each parental strand acts as a template for the synthesis of new strand of D N A, which is complementary to the parental strand. Each daughter D N A molecule always ...
... The content of parental D N A in double helix decreases with each generation according to the semi-conservative model of replication. Each parental strand acts as a template for the synthesis of new strand of D N A, which is complementary to the parental strand. Each daughter D N A molecule always ...
PCR Cloning Considerations
... the terms and conditions of all applicable Limited Use Label Licenses. All products are for research use only. CAUTION: Not intended for human or animal diagnostic or therapeutic uses. ...
... the terms and conditions of all applicable Limited Use Label Licenses. All products are for research use only. CAUTION: Not intended for human or animal diagnostic or therapeutic uses. ...
MycoplasMa Quality control
... These adverse effects can lead to the misinterpretation of results and compromise the validity of data generated for research and/or development projects. Importance of routine testing Because mycoplasma can affect the phenotypic and functional characteristics of cells in vitro, the implementation o ...
... These adverse effects can lead to the misinterpretation of results and compromise the validity of data generated for research and/or development projects. Importance of routine testing Because mycoplasma can affect the phenotypic and functional characteristics of cells in vitro, the implementation o ...
EXCHANGE OF GENETIC MATERIAL BETWEEN
... Transfer of the Hfr character from E. coli to Salmonella: It is known that the Hfr character, unlike the F+ character, is not transferable to F- cells by infection but can be transferred by conjugation (BERNSTEIN1958); in other words, the factor responsible for Hfr is located on the bacterial chromo ...
... Transfer of the Hfr character from E. coli to Salmonella: It is known that the Hfr character, unlike the F+ character, is not transferable to F- cells by infection but can be transferred by conjugation (BERNSTEIN1958); in other words, the factor responsible for Hfr is located on the bacterial chromo ...
Genit 3
... d. with genes for the same characteristics at corresponding loci. One homologous chromosome is inherited from the organism's mother; the other from the organism's father.[1] They are usually not identical. Each chromosome in the pair contains genes for the same biological features, such as eye color ...
... d. with genes for the same characteristics at corresponding loci. One homologous chromosome is inherited from the organism's mother; the other from the organism's father.[1] They are usually not identical. Each chromosome in the pair contains genes for the same biological features, such as eye color ...
Monday, November 17, 2014 Agenda: Cell Organelle Analogy
... • Prophase 1: Each chromosome dupicates and remains closely associated. These are called sister chromatids. Crossing-over can occur during the latter part of this stage. • Metaphase 1: Homologous chromosomes align at the center. • Anaphase 1: Homologous pairs separate with sister chromatids remai ...
... • Prophase 1: Each chromosome dupicates and remains closely associated. These are called sister chromatids. Crossing-over can occur during the latter part of this stage. • Metaphase 1: Homologous chromosomes align at the center. • Anaphase 1: Homologous pairs separate with sister chromatids remai ...
That have been aligned so that homologous residues are arranged
... Amino-acid sequence alignments are easier to carry out and less ambiguous than nucleotide alignments. A typical approach is to carry out the alignment at the aminoacid level and use it to generate a corresponding nucleotide sequence alignment, which can then be analyzed as usual. Numerous computer p ...
... Amino-acid sequence alignments are easier to carry out and less ambiguous than nucleotide alignments. A typical approach is to carry out the alignment at the aminoacid level and use it to generate a corresponding nucleotide sequence alignment, which can then be analyzed as usual. Numerous computer p ...
Real time PCR and it`s role in diagnosis
... changing antigens on the surface of infected red blood cells. Real-time PCR assays have the potential to detect low levels of parasitemia, identify mixed infections, and allow for precise differentiation of species via melting curve analysis.(10,11) Plasmodium detection was performed by using real-t ...
... changing antigens on the surface of infected red blood cells. Real-time PCR assays have the potential to detect low levels of parasitemia, identify mixed infections, and allow for precise differentiation of species via melting curve analysis.(10,11) Plasmodium detection was performed by using real-t ...
8. Principles of Genetics and Cytogenetics
... Historical background of genetics, theories and hypothesis. Physical basis of heredity, cell reproduction, mitosis, meiosis and its significance. Gametogenesis and syngamy in plants. Mendelian genetics–Mendel’s principles of heredity, deviation from Mendelian inheritance, pleiotropy, threshold chara ...
... Historical background of genetics, theories and hypothesis. Physical basis of heredity, cell reproduction, mitosis, meiosis and its significance. Gametogenesis and syngamy in plants. Mendelian genetics–Mendel’s principles of heredity, deviation from Mendelian inheritance, pleiotropy, threshold chara ...
phage interactions - Experimental Evolution of Communities
... (individual molecular and phenotypic changes) may result in the emergence of structures at larger scales, such as co-evolutionary dynamics and local adaptation. We capitalized on methods commonly employed in community ecology to quantify how the structure of community interaction matrices, so-called ...
... (individual molecular and phenotypic changes) may result in the emergence of structures at larger scales, such as co-evolutionary dynamics and local adaptation. We capitalized on methods commonly employed in community ecology to quantify how the structure of community interaction matrices, so-called ...
What is Sequence Alignment?
... Homology has a very specific meaning in evolutionary & computational biology - & the term is often used incorrectly For us: Homology = similarity due to descent from a common evolutionary ancestor ...
... Homology has a very specific meaning in evolutionary & computational biology - & the term is often used incorrectly For us: Homology = similarity due to descent from a common evolutionary ancestor ...
Corning® Epoxide Coated Slides Instruction Manual
... replacement of the product or a full refund. Any misuse of this product including failure to follow proper use protocols is the responsibility of the user, and Corning makes no warranty or guarantee under these circumstances. Certain arrays and/or methods of preparation, analysis or use may be cover ...
... replacement of the product or a full refund. Any misuse of this product including failure to follow proper use protocols is the responsibility of the user, and Corning makes no warranty or guarantee under these circumstances. Certain arrays and/or methods of preparation, analysis or use may be cover ...
Molecular Identification of Nematodes Manual
... to their specific “binding sites.” We use this particular form of PCR where two primers are used in the reaction. They were specifically designed from a DNA template sequence (previously obtained from the literature or personal DNA sequencing) to be complimentary to that specific sequence, they are ...
... to their specific “binding sites.” We use this particular form of PCR where two primers are used in the reaction. They were specifically designed from a DNA template sequence (previously obtained from the literature or personal DNA sequencing) to be complimentary to that specific sequence, they are ...
Gene Section USP1 (ubiquitin specific peptidase 1) Atlas of Genetics and Cytogenetics
... et al., 1998). USP1 belongs to the ubiquitin specific protease (USP) family of human deubiquitinases (DUBs). Like other members of its family, it harbours a highly conserved USP domain organization comprising a N-terminal Cys box and a C-terminal His box, which contain the catalytic residues (C90, H ...
... et al., 1998). USP1 belongs to the ubiquitin specific protease (USP) family of human deubiquitinases (DUBs). Like other members of its family, it harbours a highly conserved USP domain organization comprising a N-terminal Cys box and a C-terminal His box, which contain the catalytic residues (C90, H ...
Classification of Centers for Disease Control Group Eugonic
... of Neisseria animaloris sp. nov. and Neisseria zoodegmatis sp. nov. from related taxa Taxa: 1, N. animaloris sp. nov (22 strains); 2, N. zoodegmatis sp. nov. (15 strains); 3, N. canis LMG 8383T; 4, N. dentiae LMG 23015T; 5, U. suis (five strains; data from Vela et al., 2005). All data are from this ...
... of Neisseria animaloris sp. nov. and Neisseria zoodegmatis sp. nov. from related taxa Taxa: 1, N. animaloris sp. nov (22 strains); 2, N. zoodegmatis sp. nov. (15 strains); 3, N. canis LMG 8383T; 4, N. dentiae LMG 23015T; 5, U. suis (five strains; data from Vela et al., 2005). All data are from this ...
Molecular mechanics of the interactions of spermine with DNA: DNA
... implies stabilization of double-stranded over single-stranded forms. It was found recently that polyamines also stabilize both the left-handed Z conformation (10,11) and the right-handed A conformation (12) of nucleic acids. Stabilization of specific DNA ...
... implies stabilization of double-stranded over single-stranded forms. It was found recently that polyamines also stabilize both the left-handed Z conformation (10,11) and the right-handed A conformation (12) of nucleic acids. Stabilization of specific DNA ...
An improved protocol for pulsed-field gel electrophoresis typing of
... nucleic acid extraction. In our proposed protocol, we shortened incubation and washing times in order to avoid or reduce such degradation. The high concentrations of proteinase K used could also help to digest nucleases more efficiently. Secondly, it has been reported that a nucleolytic peracid deri ...
... nucleic acid extraction. In our proposed protocol, we shortened incubation and washing times in order to avoid or reduce such degradation. The high concentrations of proteinase K used could also help to digest nucleases more efficiently. Secondly, it has been reported that a nucleolytic peracid deri ...
Effect of Proliferating Cell Nuclear Antigen
... when PCNA is ubiquitinated, its affinity for these polymerases is increased by virtue of these motifs, and this facilitates their binding to the stalled forks. This mechanism, deduced from in vivo studies, has recently been demonstrated for pol in a reconstituted in vitro system (Zhuang et al., 200 ...
... when PCNA is ubiquitinated, its affinity for these polymerases is increased by virtue of these motifs, and this facilitates their binding to the stalled forks. This mechanism, deduced from in vivo studies, has recently been demonstrated for pol in a reconstituted in vitro system (Zhuang et al., 200 ...
Polymerase Chain Reaction In Ophthalmology
... the basic principle of replicating a piece of DNA using two primers had already been described by Gobind Khurana in 19712. The PCR is superior in terms of sensitivity, specificity and rapidity of other diagnostic tests in the armamentarium. The presence of DNA or RNA of the pathogen can directly be ...
... the basic principle of replicating a piece of DNA using two primers had already been described by Gobind Khurana in 19712. The PCR is superior in terms of sensitivity, specificity and rapidity of other diagnostic tests in the armamentarium. The presence of DNA or RNA of the pathogen can directly be ...
Parasitism and Mutualism in Wolbachia: What the
... artifact is serious enough to afflict past and recent claims including the root lies in the middle of the Wolbachia mutualists and parasites. We show that different inference methods yield different results and high bootstrap support did not equal phylogenetic accuracy. Recombination was rare among ...
... artifact is serious enough to afflict past and recent claims including the root lies in the middle of the Wolbachia mutualists and parasites. We show that different inference methods yield different results and high bootstrap support did not equal phylogenetic accuracy. Recombination was rare among ...
Cre-Lox recombination
In the field of genetics, Cre-Lox recombination is known as a site-specific recombinase technology, and is widely used to carry out deletions, insertions, translocations and inversions at specific sites in the DNA of cells. It allows the DNA modification to be targeted to a specific cell type or be triggered by a specific external stimulus. It is implemented both in eukaryotic and prokaryotic systems.The system consists of a single enzyme, Cre recombinase, that recombines a pair of short target sequences called the Lox sequences. This system can be implemented without inserting any extra supporting proteins or sequences. The Cre enzyme and the original Lox site called the LoxP sequence are derived from bacteriophage P1.Placing Lox sequences appropriately allows genes to be activated, repressed, or exchanged for other genes. At a DNA level many types of manipulations can be carried out. The activity of the Cre enzyme can be controlled so that it is expressed in a particular cell type or triggered by an external stimulus like a chemical signal or a heat shock. These targeted DNA changes are useful in cell lineage tracing and when mutants are lethal if expressed globally.The Cre-Lox system is very similar in action and in usage to the FLP-FRT recombination system.