File - Respiratory Therapy Files
... cholinergic responses. It is not to be used alone for treating an acute attack of breathing problems, as it takes some time to work and is usually given as a maintenance drug that excels the use of Albuterol or Xopenex for people with COPD. Ipratropium is only for inhalation by mouth through an inha ...
... cholinergic responses. It is not to be used alone for treating an acute attack of breathing problems, as it takes some time to work and is usually given as a maintenance drug that excels the use of Albuterol or Xopenex for people with COPD. Ipratropium is only for inhalation by mouth through an inha ...
PCR Primer Design
... The Primer Designer features a powerful, yet extremely simple, real-time interface to allow the rapid identification of theoretical ideal primers for your PCR ...
... The Primer Designer features a powerful, yet extremely simple, real-time interface to allow the rapid identification of theoretical ideal primers for your PCR ...
Post-PCR sterilization: a method to control carryover contamination
... A post-PCR sterilization process that employs a photochemical reagent should have additional properties to be used conveniently with the PCR process. First, the non-activated reagent must not interfere with primer annealing or Taq polymerase activity, and must be thermally stable to the temperatures ...
... A post-PCR sterilization process that employs a photochemical reagent should have additional properties to be used conveniently with the PCR process. First, the non-activated reagent must not interfere with primer annealing or Taq polymerase activity, and must be thermally stable to the temperatures ...
Developing expressed sequence tag libraries and
... The same number of cDNA clones was produced as for ‘Heritage’, 18,432. Because of expected low polymorphism rate in black raspberry [40–42], 4032 clones were sequenced with a final yield of 2358 high quality sequences after quality control analysis, reported as accession numbers JZ841669 through JZ8 ...
... The same number of cDNA clones was produced as for ‘Heritage’, 18,432. Because of expected low polymorphism rate in black raspberry [40–42], 4032 clones were sequenced with a final yield of 2358 high quality sequences after quality control analysis, reported as accession numbers JZ841669 through JZ8 ...
Identifying Common Genetic Variants by High
... not of clinical interest. Methods: We conducted high-resolution melting analysis on the 24 exons of the ACVRL1 and ENG genes implicated in hereditary hemorrhagic telangiectasia (HHT). DNA in samples from 13 controls and 19 patients was PCR amplified in the presence of LCGreen® I, and all 768 exons m ...
... not of clinical interest. Methods: We conducted high-resolution melting analysis on the 24 exons of the ACVRL1 and ENG genes implicated in hereditary hemorrhagic telangiectasia (HHT). DNA in samples from 13 controls and 19 patients was PCR amplified in the presence of LCGreen® I, and all 768 exons m ...
Microsoft Word (Chapter 3) - DORAS
... Analysis of the results indicated that all siderophores tested were utilised by S. meliloti. Some background growth was evident on the plates culturing S. meliloti 2011 which was due to the production and utilisation of the endogenous siderophore rhizobactin 1021. The indicator strain S. meliloti 20 ...
... Analysis of the results indicated that all siderophores tested were utilised by S. meliloti. Some background growth was evident on the plates culturing S. meliloti 2011 which was due to the production and utilisation of the endogenous siderophore rhizobactin 1021. The indicator strain S. meliloti 20 ...
“The only UNG useful in RT-PCR”
... •• Heat-labile without any addition of agents or inhibitors •• Eliminates carry-over contamination in both PCR and RT-PCR •• Enables downstream post-PCR analysis such as cloning and sequencing. ...
... •• Heat-labile without any addition of agents or inhibitors •• Eliminates carry-over contamination in both PCR and RT-PCR •• Enables downstream post-PCR analysis such as cloning and sequencing. ...
Genetic Evidence for a Silent SUC Gene in Yeast.
... 1.-Models for the structure of suco alleles. The diagrams show schematic representations (not to scale) of a chromosome carrying a SUC locus; the filled circle represents the centromere. (a) A SUC+ allele at the SUC locus is represented by an open bar. (b) A suc0 allele is depicted as a “silent” gen ...
... 1.-Models for the structure of suco alleles. The diagrams show schematic representations (not to scale) of a chromosome carrying a SUC locus; the filled circle represents the centromere. (a) A SUC+ allele at the SUC locus is represented by an open bar. (b) A suc0 allele is depicted as a “silent” gen ...
Diagnostic protocol for
... scattered singly over the fruit or several lesions may occur together with irregular contour. Exudation of resinous substances may be observed on young infected fruits. The canker never penetrates through the rind. ...
... scattered singly over the fruit or several lesions may occur together with irregular contour. Exudation of resinous substances may be observed on young infected fruits. The canker never penetrates through the rind. ...
International Plant Protection Convention Draft annex to ISPM 27
... will fluoresce bright green under the ultraviolet light of the microscope. If the positive control with known bacterium shows fluorescent rod shaped bacterial cells and the negative controls of normal serum and PBS do not, examine the sample windows for bacterial cell wall fluorescence, looking for ...
... will fluoresce bright green under the ultraviolet light of the microscope. If the positive control with known bacterium shows fluorescent rod shaped bacterial cells and the negative controls of normal serum and PBS do not, examine the sample windows for bacterial cell wall fluorescence, looking for ...
Staphylococcus aureus CC395 harbours a novel
... in JS395 were nearly identical to sequences from an S. aureus phage, GRCS, isolated from raw sewage in India,20 and a plasmid, SAP020A, isolated from a CoNS species (DDBJ/EMBL/GenBank accession number GQ900386), respectively. Together, these findings support horizontal transfer of the CRISPR locus b ...
... in JS395 were nearly identical to sequences from an S. aureus phage, GRCS, isolated from raw sewage in India,20 and a plasmid, SAP020A, isolated from a CoNS species (DDBJ/EMBL/GenBank accession number GQ900386), respectively. Together, these findings support horizontal transfer of the CRISPR locus b ...
Multiple Alignment
... order to characterize protein families, identify shared regions of homology in a multiple sequence alignment; (this happens generally when a sequence search revealed homologies to several ...
... order to characterize protein families, identify shared regions of homology in a multiple sequence alignment; (this happens generally when a sequence search revealed homologies to several ...
Biotechnology Explorer™ GMO Investigator™ Kit: A - Bio-Rad
... Applications of Real-Time PCR PCR has found so many applications in the biotechnology lab that it has been said: “PCR is to biology what petroleum is to transportation” (Pray, 2004). Although there have been many ways that PCR has been adapted for the detection of specific nucleic acids in cells, re ...
... Applications of Real-Time PCR PCR has found so many applications in the biotechnology lab that it has been said: “PCR is to biology what petroleum is to transportation” (Pray, 2004). Although there have been many ways that PCR has been adapted for the detection of specific nucleic acids in cells, re ...
Clin Infect Dis. - Repositorio Académico UPC
... The interpretation of the isolation rate of a pathogen in stool samples from children with diarrhea, compared with asymptomatic colonization, is complex. Factors that should be considered in interpretation include bacterial factors (bacteria with more virulent genes), host factors (age, genetic susc ...
... The interpretation of the isolation rate of a pathogen in stool samples from children with diarrhea, compared with asymptomatic colonization, is complex. Factors that should be considered in interpretation include bacterial factors (bacteria with more virulent genes), host factors (age, genetic susc ...
please click, ppt - Department of Statistics | Rajshahi University
... Genes are generally very numerous, and situated within the cell nucleus, where they lie in linear order along microscopic bodies called chromosomes. The chromosomes occur in similar, or homologous, pairs, where the number of pairs is constant for each species. For example, Drosophila has 4 pairs ...
... Genes are generally very numerous, and situated within the cell nucleus, where they lie in linear order along microscopic bodies called chromosomes. The chromosomes occur in similar, or homologous, pairs, where the number of pairs is constant for each species. For example, Drosophila has 4 pairs ...
Genetics
... Gene – a unit of heredity; a section of DNA sequence encoding a single protein Genome – the entire set of genes in an organism Alleles – two genes that occupy the same position on homologous chromosomes and that cover the same trait (like ‘flavors’ of a trait), such as Sw-5 and sw-5 of tomato spott ...
... Gene – a unit of heredity; a section of DNA sequence encoding a single protein Genome – the entire set of genes in an organism Alleles – two genes that occupy the same position on homologous chromosomes and that cover the same trait (like ‘flavors’ of a trait), such as Sw-5 and sw-5 of tomato spott ...
Design-O-Saur - Beyond Benign
... Decode provided information to build a genetically unique dinosaur. Understand that this is a model that could be used to look at human genetics Understand gene sequences and the results of these sequences Materials: Clay – model magic works very well Student sheets Time needed: 1 x 45-60 ...
... Decode provided information to build a genetically unique dinosaur. Understand that this is a model that could be used to look at human genetics Understand gene sequences and the results of these sequences Materials: Clay – model magic works very well Student sheets Time needed: 1 x 45-60 ...
... to the Soviet military. These included tablets against dysentery, pyophage cocktails targeting wound and other purulent infections, as well as intestiphage intended for a wide range of enteric pathogens. Much of the main building held research laboratories continually isolating new phages, upgrading ...
Achiasmate meiosis in fission yeast - Journal of Cell Science
... were stained with the use of jellyfish GFP protein. Control, rec7 and rec8 homothallic strains were transformed with the plasmid pYC551 according to the LiCl method (Moreno et al., 1991). This pREP1 expression vector-based plasmid (Maundrell, 1993) carries the sequences for the NLS-GFP protein and t ...
... were stained with the use of jellyfish GFP protein. Control, rec7 and rec8 homothallic strains were transformed with the plasmid pYC551 according to the LiCl method (Moreno et al., 1991). This pREP1 expression vector-based plasmid (Maundrell, 1993) carries the sequences for the NLS-GFP protein and t ...
BIOSENSOR (General principles and applications)
... charge is detected using ion-selective electrodes, such as pH-meters. ...
... charge is detected using ion-selective electrodes, such as pH-meters. ...
CHROMOTHRIPSIS FROM DNA DAMAGE IN MICRONUCLEI The
... chromatid. Interestingly, we sometimes detected an unaltered product in addition to the rearranged product with the missegregated haplotype. We hypothesize that these two products may be generated by breakage of a partially replicated sequence near a replication fork with only one side of the break ...
... chromatid. Interestingly, we sometimes detected an unaltered product in addition to the rearranged product with the missegregated haplotype. We hypothesize that these two products may be generated by breakage of a partially replicated sequence near a replication fork with only one side of the break ...
Downloaded - Laboratoire de Chimie Théorique
... are either potent mutagens or inhibitors. We used structural and topological analyses based on ab initio QM/MM calculations to study human DNA polymerase l (Poll) with different metals in the active site. Our results indicate a slightly longer O3 0 –Pa distance (B3.6 Å) for most inhibitor cations co ...
... are either potent mutagens or inhibitors. We used structural and topological analyses based on ab initio QM/MM calculations to study human DNA polymerase l (Poll) with different metals in the active site. Our results indicate a slightly longer O3 0 –Pa distance (B3.6 Å) for most inhibitor cations co ...
Multiple sequence alignment: outline
... (B) Each column in the model represents the possibility of a match, insert, or delete in each column of the alignment in A. The HMM is a probabilistic representation of the MSA. Sequences can be generated from the HMM by starting at the beginning state labeled BEG and then by following anyone of man ...
... (B) Each column in the model represents the possibility of a match, insert, or delete in each column of the alignment in A. The HMM is a probabilistic representation of the MSA. Sequences can be generated from the HMM by starting at the beginning state labeled BEG and then by following anyone of man ...
Cre-Lox recombination
In the field of genetics, Cre-Lox recombination is known as a site-specific recombinase technology, and is widely used to carry out deletions, insertions, translocations and inversions at specific sites in the DNA of cells. It allows the DNA modification to be targeted to a specific cell type or be triggered by a specific external stimulus. It is implemented both in eukaryotic and prokaryotic systems.The system consists of a single enzyme, Cre recombinase, that recombines a pair of short target sequences called the Lox sequences. This system can be implemented without inserting any extra supporting proteins or sequences. The Cre enzyme and the original Lox site called the LoxP sequence are derived from bacteriophage P1.Placing Lox sequences appropriately allows genes to be activated, repressed, or exchanged for other genes. At a DNA level many types of manipulations can be carried out. The activity of the Cre enzyme can be controlled so that it is expressed in a particular cell type or triggered by an external stimulus like a chemical signal or a heat shock. These targeted DNA changes are useful in cell lineage tracing and when mutants are lethal if expressed globally.The Cre-Lox system is very similar in action and in usage to the FLP-FRT recombination system.