Revisiting the Impact of Inversions in Evolution
... generate low recombination rates among the standard (noninverted) and inverted arrangements, they facilitate the spread of the coadapted alleles. Inversions carrying favorable alleles then spread to fixation unless there is migration or counteracting selection to prevent fixation. An alternative hypot ...
... generate low recombination rates among the standard (noninverted) and inverted arrangements, they facilitate the spread of the coadapted alleles. Inversions carrying favorable alleles then spread to fixation unless there is migration or counteracting selection to prevent fixation. An alternative hypot ...
Imposition of Crossover Interference through the
... density of Zip2 foci (i.e., foci/kbp) is somewhat higher for the two smaller chromosomes examined than it is for the two larger chromosomes (Table 1). SICs Display Interference As mentioned in the Introduction, a number of observations indicate that synapsis initiates at the sites of recombination e ...
... density of Zip2 foci (i.e., foci/kbp) is somewhat higher for the two smaller chromosomes examined than it is for the two larger chromosomes (Table 1). SICs Display Interference As mentioned in the Introduction, a number of observations indicate that synapsis initiates at the sites of recombination e ...
A family of thermostable fungal cellulases created by structure-guided recombination
... form. Five of these chimeras have half-lives of thermal inactivation at 63 °C that are greater than the most stable parent, CBH II enzyme from the thermophilic fungus Humicola insolens, which suggests that this chimera collection contains hundreds of highly stable cellulases. Twenty-five new sequenc ...
... form. Five of these chimeras have half-lives of thermal inactivation at 63 °C that are greater than the most stable parent, CBH II enzyme from the thermophilic fungus Humicola insolens, which suggests that this chimera collection contains hundreds of highly stable cellulases. Twenty-five new sequenc ...
Designing synthetic MLPA probes - MRC
... obtained on the Entrez Gene website: http://www.ncbi.nlm.nih.gov/sites/entrez?db=gene. For exon numbering, which cannot be found in NM_sequences anymore, look for corresponding NG_sequence here: http://www.ncbi.nlm.nih.gov/refseq/rsg/browse/. N.b. NG_sequences also include intronic sequences. Nucleo ...
... obtained on the Entrez Gene website: http://www.ncbi.nlm.nih.gov/sites/entrez?db=gene. For exon numbering, which cannot be found in NM_sequences anymore, look for corresponding NG_sequence here: http://www.ncbi.nlm.nih.gov/refseq/rsg/browse/. N.b. NG_sequences also include intronic sequences. Nucleo ...
2004-009_-Draft-Anne..
... an additional test based on a different biological principle should be used for detection. In all tests, positive and negative controls must be included. The use of products of commercial brands in this diagnostic protocol implies no approval of them to the exclusion of others that may also be suita ...
... an additional test based on a different biological principle should be used for detection. In all tests, positive and negative controls must be included. The use of products of commercial brands in this diagnostic protocol implies no approval of them to the exclusion of others that may also be suita ...
The use of genetic markers in poultry breeding
... Box 1: A genetic marker Inthisthesis a genetic marker isdefined as aparameter that has adirect relation with alleles at a genomic site. Given this definition amorphological trait or a biochemical polymorphism can be a genetic marker. For example, blood group polymorphisms have a direct relation to a ...
... Box 1: A genetic marker Inthisthesis a genetic marker isdefined as aparameter that has adirect relation with alleles at a genomic site. Given this definition amorphological trait or a biochemical polymorphism can be a genetic marker. For example, blood group polymorphisms have a direct relation to a ...
Product description P003-D1 MLH1-MSH2-v01 - MRC
... containing MLH1 or MSH2 exon deletions or amplifications: http://www.nibsc.org/documents/ifu/11-218xxx.pdf. As an alternative, an artificial positive duplication DNA sample for MLH1 and MSH2 (product name SD027) can be ordered from MRC-Holland. This FOR RESEARCH USE ONLY SD027 DNA will show a duplic ...
... containing MLH1 or MSH2 exon deletions or amplifications: http://www.nibsc.org/documents/ifu/11-218xxx.pdf. As an alternative, an artificial positive duplication DNA sample for MLH1 and MSH2 (product name SD027) can be ordered from MRC-Holland. This FOR RESEARCH USE ONLY SD027 DNA will show a duplic ...
Novel pathogen-specific primers for the detection of Agrobacterium
... 2002, GENOV et al. 2005 a). More recently further attempts were carried out with iaaH-specific primers that detected a wide range of A. tumefaciens and a few A. vitis strains (PULAWSKA and SOBICZEWSKY 2005). In spite of several attempts, no single or multiplex primer combinations are known that are a ...
... 2002, GENOV et al. 2005 a). More recently further attempts were carried out with iaaH-specific primers that detected a wide range of A. tumefaciens and a few A. vitis strains (PULAWSKA and SOBICZEWSKY 2005). In spite of several attempts, no single or multiplex primer combinations are known that are a ...
A12_ScienceAndFiction - Collegiate Quiz Bowl Packet Archive
... 13. Of interest to developmental biologists for their ability to reorganize their cells by species after being homogenized with multiple other species, they consist of only four cell types*. These cell types are able to provide the organism with functions like protection, nutrition, organization an ...
... 13. Of interest to developmental biologists for their ability to reorganize their cells by species after being homogenized with multiple other species, they consist of only four cell types*. These cell types are able to provide the organism with functions like protection, nutrition, organization an ...
The Myriad case (Association for Molecular Pathology v
... “Isolated DNA has been cleaved (i.e., had covalent bonds in its backbone chemically severed) or synthesized to consist of just a fraction of a naturally occurring DNA molecule” • Native BRCA1/2 resides on chromosomes 17/13 part of millions of nucleotides • Isolated BRCA1/2 – exons only covering as f ...
... “Isolated DNA has been cleaved (i.e., had covalent bonds in its backbone chemically severed) or synthesized to consist of just a fraction of a naturally occurring DNA molecule” • Native BRCA1/2 resides on chromosomes 17/13 part of millions of nucleotides • Isolated BRCA1/2 – exons only covering as f ...
OLSON LAB PROTOCOL: Agarose Gel Electrophoresis using GelRed
... and hence the fragments are size fractionated • The resolution of separation depends on the type and concentration of gel and the length of the run: ‚mini‘ gels are fairly low resolution and typically used for separating fragments that differ by >100 bp • By adding a fluorescent marker that is incor ...
... and hence the fragments are size fractionated • The resolution of separation depends on the type and concentration of gel and the length of the run: ‚mini‘ gels are fairly low resolution and typically used for separating fragments that differ by >100 bp • By adding a fluorescent marker that is incor ...
What is p53
... Between January 1993 and July 1996, more than 4300 papers have been published in which the term "p53" appears in the title! This massive interest in a single protein is almost unprecedented and reflects the central place of p53 in the regulation of cell number and the frequency with which abnormalit ...
... Between January 1993 and July 1996, more than 4300 papers have been published in which the term "p53" appears in the title! This massive interest in a single protein is almost unprecedented and reflects the central place of p53 in the regulation of cell number and the frequency with which abnormalit ...
International Agency for Research on Cancer (IARC)
... heavy than in those with light aflatoxin intake. The other two studies, one in Hong Kong and one in Thailand, gave negative results. In Thailand, one study on hepatocellular carcinoma and another on cholangiocarcinoma also found no association with the presence of aflatoxin B1-albumin adducts in ser ...
... heavy than in those with light aflatoxin intake. The other two studies, one in Hong Kong and one in Thailand, gave negative results. In Thailand, one study on hepatocellular carcinoma and another on cholangiocarcinoma also found no association with the presence of aflatoxin B1-albumin adducts in ser ...
A Single Eubacterial Origin of Eukaryotic
... FIG. 2.—LogDet bootstrap consensus tree of variable sites of DNA codon positions 1 and 2. Ninety-five percent of unvaried sites were estimated to be invariable using ML with a general time reversible model and two rate categories (variable/invariable), with all parameters estimated in PAUP* based on ...
... FIG. 2.—LogDet bootstrap consensus tree of variable sites of DNA codon positions 1 and 2. Ninety-five percent of unvaried sites were estimated to be invariable using ML with a general time reversible model and two rate categories (variable/invariable), with all parameters estimated in PAUP* based on ...
1 - IPPC
... indicated in Figures 1 and 2 are the minimum requirements for the diagnosis, but further tests may be required by the national plant protection organization (NPPO), especially for the first report in a country. For example, serological tests may facilitate a presumptive diagnosis of symptomatic plan ...
... indicated in Figures 1 and 2 are the minimum requirements for the diagnosis, but further tests may be required by the national plant protection organization (NPPO), especially for the first report in a country. For example, serological tests may facilitate a presumptive diagnosis of symptomatic plan ...
projects - Computer Science and Engineering
... PCR is a technique for amplifying and detecting a specific portion of the genome Amplification takes place if the primers are ‘appropriate’ distance ...
... PCR is a technique for amplifying and detecting a specific portion of the genome Amplification takes place if the primers are ‘appropriate’ distance ...
Method for identification of origins of replication and genes
... DnaA boxes are frequent around the origin, and recruit the replication machinery to that site (Messer and Weigel, 1997). The sequence of DnaA boxes is very well conserved throughout bacteria: 5'-IT1Jf TNCACA-3'1, where T, C, and A represent three out of four monomeric subunits of the DNA polymer and ...
... DnaA boxes are frequent around the origin, and recruit the replication machinery to that site (Messer and Weigel, 1997). The sequence of DnaA boxes is very well conserved throughout bacteria: 5'-IT1Jf TNCACA-3'1, where T, C, and A represent three out of four monomeric subunits of the DNA polymer and ...
One-stop polymerase chain reaction (PCR): An improved PCR
... commonly used PCR methods due to their low cost and ready availability. As a proof-of-principle test, a series of fragments of human β-actin mRNA (NM_001101) ranging from 100 to 750 bp were amplified to examine the amplification specificity and migration pattern with the presence (at final concentra ...
... commonly used PCR methods due to their low cost and ready availability. As a proof-of-principle test, a series of fragments of human β-actin mRNA (NM_001101) ranging from 100 to 750 bp were amplified to examine the amplification specificity and migration pattern with the presence (at final concentra ...
Updated slides on graph algorithms for DNA sequencing
... • Normally bacteriophage T4 (a virus) kills bacteria • However if T4 is mutated (e.g., an important gene is deleted) it gets disabled and loses an ability to kill bacteria • Suppose the bacteria is infected with two different mutants each of which is disabled – would the bacteria still survive? • Am ...
... • Normally bacteriophage T4 (a virus) kills bacteria • However if T4 is mutated (e.g., an important gene is deleted) it gets disabled and loses an ability to kill bacteria • Suppose the bacteria is infected with two different mutants each of which is disabled – would the bacteria still survive? • Am ...
Biology II Final Exam Practice
... ____ 11. A vestigial structure in one organism can be defined as a reduced form of a functional structure in another organism. ____ 12. Natural selection is based on the concepts of excess reproduction, variation, inheritance, and the advantages of certain traits. ____ 13. Darwin developed his theor ...
... ____ 11. A vestigial structure in one organism can be defined as a reduced form of a functional structure in another organism. ____ 12. Natural selection is based on the concepts of excess reproduction, variation, inheritance, and the advantages of certain traits. ____ 13. Darwin developed his theor ...
Cre-Lox recombination
In the field of genetics, Cre-Lox recombination is known as a site-specific recombinase technology, and is widely used to carry out deletions, insertions, translocations and inversions at specific sites in the DNA of cells. It allows the DNA modification to be targeted to a specific cell type or be triggered by a specific external stimulus. It is implemented both in eukaryotic and prokaryotic systems.The system consists of a single enzyme, Cre recombinase, that recombines a pair of short target sequences called the Lox sequences. This system can be implemented without inserting any extra supporting proteins or sequences. The Cre enzyme and the original Lox site called the LoxP sequence are derived from bacteriophage P1.Placing Lox sequences appropriately allows genes to be activated, repressed, or exchanged for other genes. At a DNA level many types of manipulations can be carried out. The activity of the Cre enzyme can be controlled so that it is expressed in a particular cell type or triggered by an external stimulus like a chemical signal or a heat shock. These targeted DNA changes are useful in cell lineage tracing and when mutants are lethal if expressed globally.The Cre-Lox system is very similar in action and in usage to the FLP-FRT recombination system.