Specificity of Insertion by the Translocatable Tetracycline Resistance Element Tn10.
... Since the conclusions from this analysis depend critically on the independent occurrence of each his::TnIOinsertion studied, care was taken to insure that this requirement was met. Many independent mixtures of phage and cells were made, and only one his: :TdO insertion was saved from each adsorption ...
... Since the conclusions from this analysis depend critically on the independent occurrence of each his::TnIOinsertion studied, care was taken to insure that this requirement was met. Many independent mixtures of phage and cells were made, and only one his: :TdO insertion was saved from each adsorption ...
Bosque, a software system for phylogenetic analysis
... By local we mean that this database is located (as a file) on the same computer where Bosque is executed. The main advantage (for this application) of having a local database to store everything is that we avoid the complications for the user of: 1. Manipulate computer files on different formats 2. ...
... By local we mean that this database is located (as a file) on the same computer where Bosque is executed. The main advantage (for this application) of having a local database to store everything is that we avoid the complications for the user of: 1. Manipulate computer files on different formats 2. ...
Control of Cleavage Cycles in Drosophila Embryos by fru¨ hstart
... Early metazoan development consists of cleavage stages characterized by rapid cell cycles that successively divide the fertilized egg. The cell cycle oscillator pauses when the ratio of DNA and cytoplasm (N/C) reaches a threshold characteristic for the species. This pause requires maternal factors a ...
... Early metazoan development consists of cleavage stages characterized by rapid cell cycles that successively divide the fertilized egg. The cell cycle oscillator pauses when the ratio of DNA and cytoplasm (N/C) reaches a threshold characteristic for the species. This pause requires maternal factors a ...
using polymerase chain reaction for detection of enterotoxigenic
... Key words: Enterotoxigenic Vibrio cholerae, enterotoxin, cholera toxin, polymerase chain reaction (PCR) INTRODUCTION Cholera has been one of the most feared diseases for human. It was recognized that two biotypes were agglutinated by a single antiserum designated O1 in the 1930s. Other strains of Vi ...
... Key words: Enterotoxigenic Vibrio cholerae, enterotoxin, cholera toxin, polymerase chain reaction (PCR) INTRODUCTION Cholera has been one of the most feared diseases for human. It was recognized that two biotypes were agglutinated by a single antiserum designated O1 in the 1930s. Other strains of Vi ...
Table of Contents - Scholars` Bank
... forms proteins, which are essential for most cellular functions. Proteins are molecules composed of smaller molecules, called amino acids, that link together and fold into a certain shape that dictates the protein’s function. The process of protein formation begins with a cell's database, its DNA. D ...
... forms proteins, which are essential for most cellular functions. Proteins are molecules composed of smaller molecules, called amino acids, that link together and fold into a certain shape that dictates the protein’s function. The process of protein formation begins with a cell's database, its DNA. D ...
Universal chloroplast integration and expression vectors
... The invention provides universal chloroplast integration and expression vectors which are competent to stably transform and integrate genes of interest into chloroplast genome of multiple species of plants. Transformed plants and their progeny are provided. Monocotyledonous and dicotyledonous plants ...
... The invention provides universal chloroplast integration and expression vectors which are competent to stably transform and integrate genes of interest into chloroplast genome of multiple species of plants. Transformed plants and their progeny are provided. Monocotyledonous and dicotyledonous plants ...
cloning of gs1 gene encodes glutamine synthetase 1 and
... Glutamine synthetase (EC 6.3.1.2) is an enzyme which assimilates ammonium into amino acids. Therefore, GS plays an essential role in the metabolism of nitrogen. In vascular plants, GS is devided into two groups: cytosol - located isoform (GS1) and plastid – located isoform (GS2). Surveys have shown ...
... Glutamine synthetase (EC 6.3.1.2) is an enzyme which assimilates ammonium into amino acids. Therefore, GS plays an essential role in the metabolism of nitrogen. In vascular plants, GS is devided into two groups: cytosol - located isoform (GS1) and plastid – located isoform (GS2). Surveys have shown ...
... into close proximity [11-13]. 3C technology detects interactions between DNA regions by in vivo crosslinking of chromatin and PCR with primers specific for novel junctions created by ligation of sequences that were in close proximity through specific interactions. To determine if the Kcnq1 promoter ...
Fanconi anemia and RAD50 deficiency: genetic and functional
... oxygen species and other DNA damaging agents. FA is caused by biallelic mutations in at least 11 different genes which appear to function in the maintenance of genomic stability. Eight of the FA genes form a nuclear core complex with a catalytic function involving ubiquitination of the central FANCD ...
... oxygen species and other DNA damaging agents. FA is caused by biallelic mutations in at least 11 different genes which appear to function in the maintenance of genomic stability. Eight of the FA genes form a nuclear core complex with a catalytic function involving ubiquitination of the central FANCD ...
A Comparative Study on the Recovery of EcoRI Endonuclease from
... preparation of recombinant molecules, and they provide an attractive system for the analysis of sequence specific DNA-protein interactions1 . Escherichia coli RI (EcoRI) endonuclease is a well-known restriction enzyme that recognizes the symmetrical hexanucleotide sequence GAATTC on duplex DNA and c ...
... preparation of recombinant molecules, and they provide an attractive system for the analysis of sequence specific DNA-protein interactions1 . Escherichia coli RI (EcoRI) endonuclease is a well-known restriction enzyme that recognizes the symmetrical hexanucleotide sequence GAATTC on duplex DNA and c ...
one-step and stepwise magnification of a bobbed lethal
... regions (Figure lb). Similar observations have been made for other Y chromosomes stained with Hoechst 33258 or quinacrine (GATTI, PIMPINELLI and SANTINI1976; GATTI and PIMPINELLI1983). Three of the one-step y bb" chromosomes, 10, 35 and 48, appeared as acrocentric chromosomes with a uniformly staini ...
... regions (Figure lb). Similar observations have been made for other Y chromosomes stained with Hoechst 33258 or quinacrine (GATTI, PIMPINELLI and SANTINI1976; GATTI and PIMPINELLI1983). Three of the one-step y bb" chromosomes, 10, 35 and 48, appeared as acrocentric chromosomes with a uniformly staini ...
Chance and Necessity in Arthur Peacocke`s Scientific Work
... branched or forked or just a single helix, whether once disassociated (denatured) DNA strands would re-associate (renature) and other similar questions. Peacocke’s studies with intercalating dyes set the stage for his own discoveries in this area. Furthermore, Peacocke investigated the denaturation ...
... branched or forked or just a single helix, whether once disassociated (denatured) DNA strands would re-associate (renature) and other similar questions. Peacocke’s studies with intercalating dyes set the stage for his own discoveries in this area. Furthermore, Peacocke investigated the denaturation ...
paper - Université de Namur
... provided by the CCV theory. For the first time, I discuss comparatively the diffraction patterns of both the A-DNA and B-DNA conformations which are two distinct DNA structures reversibly adopted by the molecule depending on the water content of the fibre. Discussing simultaneously the A and B patte ...
... provided by the CCV theory. For the first time, I discuss comparatively the diffraction patterns of both the A-DNA and B-DNA conformations which are two distinct DNA structures reversibly adopted by the molecule depending on the water content of the fibre. Discussing simultaneously the A and B patte ...
Identification and isolation of active N2O reducers in rice paddy soil
... for N2O reduction. For the stable isotope probing (SIP) experiment, 13C-labeled succinate was used to identify succinate-assimilating microbes under N2O-reducing conditions. DNA was extracted 24 h after incubation, and heavy and light DNA fractions were separated by density gradient ultracentrifugat ...
... for N2O reduction. For the stable isotope probing (SIP) experiment, 13C-labeled succinate was used to identify succinate-assimilating microbes under N2O-reducing conditions. DNA was extracted 24 h after incubation, and heavy and light DNA fractions were separated by density gradient ultracentrifugat ...
Genetics Principles And Analysis
... Connection: Is a Bacteriophage an "Organism"? Alfred D. Hershey and Raquel Rotman 1948 Genetic recombination between host-range and plaque-type mutants of bacteriophage in single bacterial cells ...
... Connection: Is a Bacteriophage an "Organism"? Alfred D. Hershey and Raquel Rotman 1948 Genetic recombination between host-range and plaque-type mutants of bacteriophage in single bacterial cells ...
Studies on the Mechanisms of Homolog Pairing and Sister
... meiosis I and sister chromatids during meiosis II is essential for the survival of the offspring. Aberrant chromosome segregation at any stage of meiosis can lead to aneuploidy. Meiotic chromosome segregation without crossing over or chiasmata is a widespread but poorly understand chromosome segrega ...
... meiosis I and sister chromatids during meiosis II is essential for the survival of the offspring. Aberrant chromosome segregation at any stage of meiosis can lead to aneuploidy. Meiotic chromosome segregation without crossing over or chiasmata is a widespread but poorly understand chromosome segrega ...
Plant Molecular Biology
... the infection thread. Experiments using transcriptional lacZ fusions suggest that nodL belongs to the same transcriptional unit as nodF,E. Very low expression of nodL seems to be sufficient for biological activity. Introduction ...
... the infection thread. Experiments using transcriptional lacZ fusions suggest that nodL belongs to the same transcriptional unit as nodF,E. Very low expression of nodL seems to be sufficient for biological activity. Introduction ...
Methods of Human Heredity Study
... chromomeres, chromocentres and knobs. Chromomeres are regular features of all prophase chromosomes, larger enough to reveal them, but their number, size; distribution and arrangement are specific for a particular species at a particular stage of development. Chromocentres are heterochromatic regions ...
... chromomeres, chromocentres and knobs. Chromomeres are regular features of all prophase chromosomes, larger enough to reveal them, but their number, size; distribution and arrangement are specific for a particular species at a particular stage of development. Chromocentres are heterochromatic regions ...
Careful risk assessment needed to evaluate transgenic fish
... transgenic fish were produced in 1984, and since that time more than 30 species have been genetically engineered worldwide (table 1). The number of transgenic species is higher for fish than for all other vertebrate species combined. Transgenic fish have been developed for applications such as the prod ...
... transgenic fish were produced in 1984, and since that time more than 30 species have been genetically engineered worldwide (table 1). The number of transgenic species is higher for fish than for all other vertebrate species combined. Transgenic fish have been developed for applications such as the prod ...
to view
... is very small. Why such type of adjustment is there in higher organisms? Ans.Female gamete is large and non motile, is an adaptation for storing more food which will be required for the future development. The male gamete has to move to reach the counterpart, so it has the machinery for its reaching ...
... is very small. Why such type of adjustment is there in higher organisms? Ans.Female gamete is large and non motile, is an adaptation for storing more food which will be required for the future development. The male gamete has to move to reach the counterpart, so it has the machinery for its reaching ...
Cre-Lox recombination
In the field of genetics, Cre-Lox recombination is known as a site-specific recombinase technology, and is widely used to carry out deletions, insertions, translocations and inversions at specific sites in the DNA of cells. It allows the DNA modification to be targeted to a specific cell type or be triggered by a specific external stimulus. It is implemented both in eukaryotic and prokaryotic systems.The system consists of a single enzyme, Cre recombinase, that recombines a pair of short target sequences called the Lox sequences. This system can be implemented without inserting any extra supporting proteins or sequences. The Cre enzyme and the original Lox site called the LoxP sequence are derived from bacteriophage P1.Placing Lox sequences appropriately allows genes to be activated, repressed, or exchanged for other genes. At a DNA level many types of manipulations can be carried out. The activity of the Cre enzyme can be controlled so that it is expressed in a particular cell type or triggered by an external stimulus like a chemical signal or a heat shock. These targeted DNA changes are useful in cell lineage tracing and when mutants are lethal if expressed globally.The Cre-Lox system is very similar in action and in usage to the FLP-FRT recombination system.