Directional Positive Selection on an Allele of Arbitrary
... the next event, t, is given by solving exp( z aðtÞdt) ¼ 1 U , where U is a uniform random number. The next event at time z 1 t is chosen randomly with probability ak ðz 1 tÞ=aðz 1 tÞ, where ak ðz 1 tÞ is the instantaneous rate of event k (e.g., recombination within the favored class), and aðz 1 t ...
... the next event, t, is given by solving exp( z aðtÞdt) ¼ 1 U , where U is a uniform random number. The next event at time z 1 t is chosen randomly with probability ak ðz 1 tÞ=aðz 1 tÞ, where ak ðz 1 tÞ is the instantaneous rate of event k (e.g., recombination within the favored class), and aðz 1 t ...
Construction and Characterization of a Highly Regulable Expression
... vectors rely on lac control in order to overproduce a gene of choice (Brosius, 1988; Balbás and Bolivar, 1990). With the wild-type lac promoter/operator, induction ratios of up to 1000⫻ have been observed between repressed versus induced growth conditions (Beckwith and Zipser, 1970). The lac promot ...
... vectors rely on lac control in order to overproduce a gene of choice (Brosius, 1988; Balbás and Bolivar, 1990). With the wild-type lac promoter/operator, induction ratios of up to 1000⫻ have been observed between repressed versus induced growth conditions (Beckwith and Zipser, 1970). The lac promot ...
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... and N2O formation under normal physiological conditions, whether through the decomposition of an unstable intermediate in the oxidation of NH2OH to NO2⫺ or purely from NO2⫺ reduction, was unclear until the use of 15N tracers demonstrated that N2O was derived primarily from NO2⫺ (33, 35). The partial ...
... and N2O formation under normal physiological conditions, whether through the decomposition of an unstable intermediate in the oxidation of NH2OH to NO2⫺ or purely from NO2⫺ reduction, was unclear until the use of 15N tracers demonstrated that N2O was derived primarily from NO2⫺ (33, 35). The partial ...
pyrimidine
... Laser fluorescence detection allows for primer identification in real time An automated sequencing machine can handle 4500 bases/hour That’s one of the technologies that has made large-scale sequencing projects like the human genome project possible ...
... Laser fluorescence detection allows for primer identification in real time An automated sequencing machine can handle 4500 bases/hour That’s one of the technologies that has made large-scale sequencing projects like the human genome project possible ...
Spring 2016 Biology Item Release Scoring Guide
... knowledge. Communicate with clarity, focus and organization using rich, investigative scenarios, real-world data and valid scientific information. ...
... knowledge. Communicate with clarity, focus and organization using rich, investigative scenarios, real-world data and valid scientific information. ...
Brand, Veronica - Degenerate Primer Design using Computational Tools
... parameters for efficient PCR analysis. It has a different purpose than HYDEN; its goal is not so much to optimize one degenerate primer pair for a set of sequences, but to look at a set of primers as a whole. As such, each primer can be a little more specific for its target sequence. This method ca ...
... parameters for efficient PCR analysis. It has a different purpose than HYDEN; its goal is not so much to optimize one degenerate primer pair for a set of sequences, but to look at a set of primers as a whole. As such, each primer can be a little more specific for its target sequence. This method ca ...
PhytoREF: a reference database of the plastidial 16S
... 2011); (ii) ribosomal DNA of large protist cells (mainly heterotrophs and potentially multinucleated) or metazoans tends to be preferentially PCR-amplified because of the relatively higher copy number of ribosomal genes in these organisms (Zhu et al. 2005; Godhe et al. 2008); (iii) distinction betwe ...
... 2011); (ii) ribosomal DNA of large protist cells (mainly heterotrophs and potentially multinucleated) or metazoans tends to be preferentially PCR-amplified because of the relatively higher copy number of ribosomal genes in these organisms (Zhu et al. 2005; Godhe et al. 2008); (iii) distinction betwe ...
Harvey ras (H-ras) Point Mutations Are Induced by 4
... DMBA, and the promoter, TPA (12, 13). Continuous exposure of squamous cells to DMBA and TPA induced H-ras mutations on chromosome 7 in greater than 90% of mice (14). H-ras appeared to be activated by specific mutations which can be affected by the initiating carcinogen (15). Since tumors do not deve ...
... DMBA, and the promoter, TPA (12, 13). Continuous exposure of squamous cells to DMBA and TPA induced H-ras mutations on chromosome 7 in greater than 90% of mice (14). H-ras appeared to be activated by specific mutations which can be affected by the initiating carcinogen (15). Since tumors do not deve ...
Biotechnology Timeline
... Advances in 3D printing technology lead to “skin printing.” European scientists begin clinical trials for an anti-HIV biotech medicine produced using genetically modified tobacco. This increases the potential for cost-effective HIV/AIDS therapy in the developing world. ...
... Advances in 3D printing technology lead to “skin printing.” European scientists begin clinical trials for an anti-HIV biotech medicine produced using genetically modified tobacco. This increases the potential for cost-effective HIV/AIDS therapy in the developing world. ...
Pourcel et al., Microbiology 2005
... Yersin (1894), strains of biovar Antiqua have been generally isolated from Asia and Africa; Medievalis was found in Central Asia, and Orientalis worldwide. Pestoides are particular strains isolated in Central Asia, and have never been found associated with disease in humans (Anisimov et al., 2004). ...
... Yersin (1894), strains of biovar Antiqua have been generally isolated from Asia and Africa; Medievalis was found in Central Asia, and Orientalis worldwide. Pestoides are particular strains isolated in Central Asia, and have never been found associated with disease in humans (Anisimov et al., 2004). ...
Control of DNA excision efficiency in Paramecium
... nuclei then differentiate into a micronucleus or a macronucleus. In the second case, the whole genome is processed through chromosome fragmentation, telomere addition to the broken ends and excision of internal eliminated sequences (IESs) that are eventually eliminated. Massive DNA amplification als ...
... nuclei then differentiate into a micronucleus or a macronucleus. In the second case, the whole genome is processed through chromosome fragmentation, telomere addition to the broken ends and excision of internal eliminated sequences (IESs) that are eventually eliminated. Massive DNA amplification als ...
FEMS Microbiology Letters
... In the pyruvate clastic reaction [1], PFO catalyses the oxidative cleavage of pyruvate and coenzyme A to acetylcoenzyme A and CO2 with concomittant reduction of ferredoxin. The enzyme has been described for strictly anaerobic bacteria like Clostridium spp. [1], diverse archaea [2] and some eukaryote ...
... In the pyruvate clastic reaction [1], PFO catalyses the oxidative cleavage of pyruvate and coenzyme A to acetylcoenzyme A and CO2 with concomittant reduction of ferredoxin. The enzyme has been described for strictly anaerobic bacteria like Clostridium spp. [1], diverse archaea [2] and some eukaryote ...
About the origins of Molecular Biology and of our Department at UTD
... Günther Stent came from Berkeley, and Luria in Nashville had sent his student Jim Watson. Also Bresch came; all wanted to experience the Bohr-inspired “Copenhagen spirit”. At that time it was thought that genes are proteins, not DNA, but Watson wanted to get away from proteins and Copenhagen and mov ...
... Günther Stent came from Berkeley, and Luria in Nashville had sent his student Jim Watson. Also Bresch came; all wanted to experience the Bohr-inspired “Copenhagen spirit”. At that time it was thought that genes are proteins, not DNA, but Watson wanted to get away from proteins and Copenhagen and mov ...
background of the invention
... With reference to FIG. 2 for a schematic view showing the layout of a field-effect transistor chip of a field-effect transistor type biosensor in accordance with an embodiment of the present invention, a field-effect transistor chip is fabrication by an n-well complementary metal oxide semiconductor ...
... With reference to FIG. 2 for a schematic view showing the layout of a field-effect transistor chip of a field-effect transistor type biosensor in accordance with an embodiment of the present invention, a field-effect transistor chip is fabrication by an n-well complementary metal oxide semiconductor ...
Distinguishing Different DNA Heterozygotes by
... isothermal probe-based assays. Hybridization probe assays that interrogate over a range of temperatures (melting assays) often detect the presence of unexpected polymorphisms but may require further studies to identify them. In our study, high-resolution melting of small amplicons distinguished all ...
... isothermal probe-based assays. Hybridization probe assays that interrogate over a range of temperatures (melting assays) often detect the presence of unexpected polymorphisms but may require further studies to identify them. In our study, high-resolution melting of small amplicons distinguished all ...
Protein export elements from Lactococcus lactis
... proteins, it has been shown that Lactobacillusplantarum is able to secreteBacillus stearothermophilusa-amylase and Clostridium thermocellumcellulase A, using the native signal peptides of these proteins (Scheilink et al. 1989). The secretion by Lactococcus lactis of Bacillus subtilis neutral proteas ...
... proteins, it has been shown that Lactobacillusplantarum is able to secreteBacillus stearothermophilusa-amylase and Clostridium thermocellumcellulase A, using the native signal peptides of these proteins (Scheilink et al. 1989). The secretion by Lactococcus lactis of Bacillus subtilis neutral proteas ...
Mitochondrial DNA (mtDNA) Biogenesis: Visualization and Duel
... and Mitchison 2008). In addition, the labeling procedure for EdU does not involve the harsh acid or enzyme digests needed for BrdU epitope recovery, and therefore allows greater flexibility and more comprehensive results by comparing its incorporation with that of other intracellular markers (Cappel ...
... and Mitchison 2008). In addition, the labeling procedure for EdU does not involve the harsh acid or enzyme digests needed for BrdU epitope recovery, and therefore allows greater flexibility and more comprehensive results by comparing its incorporation with that of other intracellular markers (Cappel ...
TAP Express® Kit
... We congratulate you on your purchase of this exclusive, high-performance product. With this kit, you will be able to transform any gene-of-interest into a transcriptionally active PCR (TAP) fragment that is ready for direct introduction into cells or animals. Because of its simplicity and speed, the ...
... We congratulate you on your purchase of this exclusive, high-performance product. With this kit, you will be able to transform any gene-of-interest into a transcriptionally active PCR (TAP) fragment that is ready for direct introduction into cells or animals. Because of its simplicity and speed, the ...
Databases_what_and_w..
... • Proteins come from the DNA via RNA. • Changes in DNA cause changes in proteins. • Changes in proteins cause changes in phenotypes. ...
... • Proteins come from the DNA via RNA. • Changes in DNA cause changes in proteins. • Changes in proteins cause changes in phenotypes. ...
Structure, expression and chromosomal location of the Oct
... other members of the POU family. After digestion with PstI, a restriction fragment length polymorphism (RFLP) was identified in C57BL/6 (B6) versus AKR and A / J . This allowed use of the congenic chromosomes listed in Fig. 1A (Flaherty et al., 1990). Whereas, the B6.K1 and B6.K3 recombinants place ...
... other members of the POU family. After digestion with PstI, a restriction fragment length polymorphism (RFLP) was identified in C57BL/6 (B6) versus AKR and A / J . This allowed use of the congenic chromosomes listed in Fig. 1A (Flaherty et al., 1990). Whereas, the B6.K1 and B6.K3 recombinants place ...
Statistical analysis of simple repeats in the human genome
... base pairs (bp) long, among which belong the Alu families. A link between homo- and dipolymeric tracts and mobile elements has been recently highlighted. In particular, the mobility of Alu repeats, which form 10% of the human genome, has been correlated with the length of poly(A) tracts located at o ...
... base pairs (bp) long, among which belong the Alu families. A link between homo- and dipolymeric tracts and mobile elements has been recently highlighted. In particular, the mobility of Alu repeats, which form 10% of the human genome, has been correlated with the length of poly(A) tracts located at o ...
Cre-Lox recombination
In the field of genetics, Cre-Lox recombination is known as a site-specific recombinase technology, and is widely used to carry out deletions, insertions, translocations and inversions at specific sites in the DNA of cells. It allows the DNA modification to be targeted to a specific cell type or be triggered by a specific external stimulus. It is implemented both in eukaryotic and prokaryotic systems.The system consists of a single enzyme, Cre recombinase, that recombines a pair of short target sequences called the Lox sequences. This system can be implemented without inserting any extra supporting proteins or sequences. The Cre enzyme and the original Lox site called the LoxP sequence are derived from bacteriophage P1.Placing Lox sequences appropriately allows genes to be activated, repressed, or exchanged for other genes. At a DNA level many types of manipulations can be carried out. The activity of the Cre enzyme can be controlled so that it is expressed in a particular cell type or triggered by an external stimulus like a chemical signal or a heat shock. These targeted DNA changes are useful in cell lineage tracing and when mutants are lethal if expressed globally.The Cre-Lox system is very similar in action and in usage to the FLP-FRT recombination system.