Regulation of 6sg expression site transcription and switching in
... promoter manipulations at 6sg expression sites (ES) and examination of the behavior of ES promoters in ectopic locations both within the ES and at other loci. In summary, ES promoter sequences inserted into non-transcribed rRNA spacers are generally inactive, or have low activity, in bloodstream and ...
... promoter manipulations at 6sg expression sites (ES) and examination of the behavior of ES promoters in ectopic locations both within the ES and at other loci. In summary, ES promoter sequences inserted into non-transcribed rRNA spacers are generally inactive, or have low activity, in bloodstream and ...
PPT - Altogen Biosystems
... The NIH3T3 cell line was established in 1962 by researchers at the New York University School of Medicine. It was derived from cells taken from a Swiss mouse embryo. The 3T3 cell line is a type of murine fibroblast cell and is now the classic fibroblast cell line in research. Furthermore, the cell l ...
... The NIH3T3 cell line was established in 1962 by researchers at the New York University School of Medicine. It was derived from cells taken from a Swiss mouse embryo. The 3T3 cell line is a type of murine fibroblast cell and is now the classic fibroblast cell line in research. Furthermore, the cell l ...
Full-Text PDF
... to different antibiotic classes and can be used to direct therapy, but are slow and take up to 72 h. In the absence of rapid results, treatment will often use broad-spectrum antibiotics on an empirical basis, which may contribute to increased drug resistance. The emergence of multi-drug-resistant (M ...
... to different antibiotic classes and can be used to direct therapy, but are slow and take up to 72 h. In the absence of rapid results, treatment will often use broad-spectrum antibiotics on an empirical basis, which may contribute to increased drug resistance. The emergence of multi-drug-resistant (M ...
introduction - Journal | Unair
... This research was looking for a specific, sensitive and accurate method by comparing DNA amplification method between PCR Silver Nitrate staining with PCR using commercial kit (Amplicor, Roche), which also can be used as a standard according to FDA. The result of this experiment showed that in sampl ...
... This research was looking for a specific, sensitive and accurate method by comparing DNA amplification method between PCR Silver Nitrate staining with PCR using commercial kit (Amplicor, Roche), which also can be used as a standard according to FDA. The result of this experiment showed that in sampl ...
The Co-Evolution of Genes and Culture Pedigrees and the
... 2. Identify and circle all the nucleotides that differ between at least two sequences in each table. 3. Pretend that you are the researcher who discovered the variations and you are writing to a colleague describing what you found. a) How would you describe the variation(s) you found in Sequence 1? ...
... 2. Identify and circle all the nucleotides that differ between at least two sequences in each table. 3. Pretend that you are the researcher who discovered the variations and you are writing to a colleague describing what you found. a) How would you describe the variation(s) you found in Sequence 1? ...
mMESSAGE mMACHINE® Kit User Guide
... mMESSAGE mMACHINE® Kits are designed for the in vitro synthesis of large amounts of capped RNA. Capped RNA mimics most eukaryotic mRNAs found in vivo, because it has a 7-methyl guanosine cap structure at the 5' end. mMESSAGE mMACHINE® Kit reactions include cap analog [m7G(5')ppp(5')G] in an ultra hi ...
... mMESSAGE mMACHINE® Kits are designed for the in vitro synthesis of large amounts of capped RNA. Capped RNA mimics most eukaryotic mRNAs found in vivo, because it has a 7-methyl guanosine cap structure at the 5' end. mMESSAGE mMACHINE® Kit reactions include cap analog [m7G(5')ppp(5')G] in an ultra hi ...
Mutualism and asexual reproduction influence recognition genes in a fungal... M.A. van der Nest1, E.T. Steenkamp2, P.M.... Wingfield1 and B. Slippers1*
... costly than asexual reproduction (e.g., Barton and Charlesworth 1998; Otto 2003). This costly reproductive strategy could be maintained because recombination acts to provide advantageous genotypes necessary for adaptation to changing environments or because recombination acts to eliminate deleteriou ...
... costly than asexual reproduction (e.g., Barton and Charlesworth 1998; Otto 2003). This costly reproductive strategy could be maintained because recombination acts to provide advantageous genotypes necessary for adaptation to changing environments or because recombination acts to eliminate deleteriou ...
PPT - Altogen Biosystems
... 5. Optional: Add 2 µl of Complex Condenser. This reagent reduces the size of transfection complex, therefore increasing transfection efficiency; however it may increase cell toxicity 6. Add prepared transfection complexes to 0.5 ml of complete growth medium with PANC-1 cells (from step 2) 7. Incubat ...
... 5. Optional: Add 2 µl of Complex Condenser. This reagent reduces the size of transfection complex, therefore increasing transfection efficiency; however it may increase cell toxicity 6. Add prepared transfection complexes to 0.5 ml of complete growth medium with PANC-1 cells (from step 2) 7. Incubat ...
Engineering of steroid biotransformation in rhodococcus van
... Total KSTD activity in R. erythropolis strain SQ1 represents two isoenzymes, named KSTD1 and KSTD2. The kstD gene, encoding the KSTD1 isoenzyme, was cloned on a 6 kb BglII genomic fragment isolated from a partial (BglII) genomic library of R. erythropolis strain SQ1 using a degenerate kstD oligonucl ...
... Total KSTD activity in R. erythropolis strain SQ1 represents two isoenzymes, named KSTD1 and KSTD2. The kstD gene, encoding the KSTD1 isoenzyme, was cloned on a 6 kb BglII genomic fragment isolated from a partial (BglII) genomic library of R. erythropolis strain SQ1 using a degenerate kstD oligonucl ...
Linkage Analysis Package
... constructed in (1) and (2) serve as input either directly to the LINKAGE programs or to the LINKAGE CONTROL PROGRAM (LCP), which is a shell for generating command files to control the analysis programs. The LINKAGE programs contain constants that determine upper limits to the number of loci, alleles ...
... constructed in (1) and (2) serve as input either directly to the LINKAGE programs or to the LINKAGE CONTROL PROGRAM (LCP), which is a shell for generating command files to control the analysis programs. The LINKAGE programs contain constants that determine upper limits to the number of loci, alleles ...
Sickle Cell Anemia - University of Washington Department of
... There are approximately 280 million hemoglobin molecules in each red blood cell (RBC). The protein portion of hemoglobin consists of four globin subunits: two alpha (α) and two beta (β ). These two types of subunits are encoded by the α and β globin genes, respectively. While the binding of oxygen a ...
... There are approximately 280 million hemoglobin molecules in each red blood cell (RBC). The protein portion of hemoglobin consists of four globin subunits: two alpha (α) and two beta (β ). These two types of subunits are encoded by the α and β globin genes, respectively. While the binding of oxygen a ...
in a patient with Ambras syndrome
... We used over twenty FISH probes to characterize the cytogenetic rearrangement in this patient and to precisely map the 8q inversion breakpoint. Comparison of the hybridization signals obtained from the normal and the rearranged chromosomes 8 enabled us to determine which clones were proximal and whi ...
... We used over twenty FISH probes to characterize the cytogenetic rearrangement in this patient and to precisely map the 8q inversion breakpoint. Comparison of the hybridization signals obtained from the normal and the rearranged chromosomes 8 enabled us to determine which clones were proximal and whi ...
New and Redesigned pRS Plasmid Shuttle Vectors for Genetic
... replacement. Hence, researchers may find themselves employing two or more pairs of oligonucleotides to replace a particular gene sequence with markers from different plasmid series. In this report, we describe our attempts to overcome the limitations described above. First, we have constructed new HI ...
... replacement. Hence, researchers may find themselves employing two or more pairs of oligonucleotides to replace a particular gene sequence with markers from different plasmid series. In this report, we describe our attempts to overcome the limitations described above. First, we have constructed new HI ...
Test Info Sheet
... 3. Genetic counseling 4. Prenatal diagnosis for known familial mutation(s) in at-risk pregnancies Methods: Using genomic DNA, coding exons and flanking splice junctions of the genes on this panel are enriched using a proprietary targeted capture method developed by GeneDx. The products are sequenced ...
... 3. Genetic counseling 4. Prenatal diagnosis for known familial mutation(s) in at-risk pregnancies Methods: Using genomic DNA, coding exons and flanking splice junctions of the genes on this panel are enriched using a proprietary targeted capture method developed by GeneDx. The products are sequenced ...
Structure and function of the GINS complex, a key component of the
... and DDK substrates is key to understanding how replication initiation is regulated. S-CDK substrates are abundant in yeast but two essential proteins, Sld2 and Sld3, have been shown to comprise the minimal set required for replication initiation [21,22]. Phosphorylation of Sld3 by S-CDK on Thr600 an ...
... and DDK substrates is key to understanding how replication initiation is regulated. S-CDK substrates are abundant in yeast but two essential proteins, Sld2 and Sld3, have been shown to comprise the minimal set required for replication initiation [21,22]. Phosphorylation of Sld3 by S-CDK on Thr600 an ...
Arc Diagrams: Visualizing Structure in Strings
... noisy data in the sense that exact repetition on a large scale is uncommon due to mutations. In some situations, ...
... noisy data in the sense that exact repetition on a large scale is uncommon due to mutations. In some situations, ...
illustra bacteria genomicPrep Mini Spin Kit
... Fig 4. Real-time PCR amplification E. coli DH5a gDNA purified with illustra bacteria genomicPrep Mini Kit and QIAamp DNA Mini Kit. Real-time PCR assays were performed using puReTaq™ RTG PCR Beads and the primer sets for the E. coli 16S rRNA gene. One microliter of the eluted gDNA solution, corresp ...
... Fig 4. Real-time PCR amplification E. coli DH5a gDNA purified with illustra bacteria genomicPrep Mini Kit and QIAamp DNA Mini Kit. Real-time PCR assays were performed using puReTaq™ RTG PCR Beads and the primer sets for the E. coli 16S rRNA gene. One microliter of the eluted gDNA solution, corresp ...
Promega Notes 49: Firefly Luciferase Engineered for Improved
... sequences for transcription factor binding sites (5) and removed many sites which could potentially interact with common factors. In some cases where it was convenient in our modification strategy, we also removed less common potential regulatory sites. As with the removal of restriction sites, the ...
... sequences for transcription factor binding sites (5) and removed many sites which could potentially interact with common factors. In some cases where it was convenient in our modification strategy, we also removed less common potential regulatory sites. As with the removal of restriction sites, the ...
Genetic Control of X Chromosome Inactivation in Mice: Definition of
... In early mammalian development, one of the two X chromosomes is silenced in each female cell as a result of X chromosome inactivation, the mammalian dosage compensation mechanism. In the mouse epiblast, the choice of which chromosome is inactivated is essentially random, but can be biased by alleles ...
... In early mammalian development, one of the two X chromosomes is silenced in each female cell as a result of X chromosome inactivation, the mammalian dosage compensation mechanism. In the mouse epiblast, the choice of which chromosome is inactivated is essentially random, but can be biased by alleles ...
Elsevier Editorial System(tm) for Diagnostic Microbiology and
... been described as etiological agents of meningitis, especially in premature infants, sepsis, bacteremia, pneumonia and endocarditis (Jean et al., 2014; de Silva et al., 2013). Carbapenem resistance in E. meningoseptica is mainly due to the production of metallo-β-lactamases (MBLs) as GOB and BlaB va ...
... been described as etiological agents of meningitis, especially in premature infants, sepsis, bacteremia, pneumonia and endocarditis (Jean et al., 2014; de Silva et al., 2013). Carbapenem resistance in E. meningoseptica is mainly due to the production of metallo-β-lactamases (MBLs) as GOB and BlaB va ...
Cre-Lox recombination
In the field of genetics, Cre-Lox recombination is known as a site-specific recombinase technology, and is widely used to carry out deletions, insertions, translocations and inversions at specific sites in the DNA of cells. It allows the DNA modification to be targeted to a specific cell type or be triggered by a specific external stimulus. It is implemented both in eukaryotic and prokaryotic systems.The system consists of a single enzyme, Cre recombinase, that recombines a pair of short target sequences called the Lox sequences. This system can be implemented without inserting any extra supporting proteins or sequences. The Cre enzyme and the original Lox site called the LoxP sequence are derived from bacteriophage P1.Placing Lox sequences appropriately allows genes to be activated, repressed, or exchanged for other genes. At a DNA level many types of manipulations can be carried out. The activity of the Cre enzyme can be controlled so that it is expressed in a particular cell type or triggered by an external stimulus like a chemical signal or a heat shock. These targeted DNA changes are useful in cell lineage tracing and when mutants are lethal if expressed globally.The Cre-Lox system is very similar in action and in usage to the FLP-FRT recombination system.