Basic information on pathways
... Methylation: Transfer of methyl groups from one chemical to another is called methylation. Essentially any chemical compound that has a methyl group as part of its chemical structure is capable of donating it to another chemical that needs it. The chemical that receives the methyl group is "methylat ...
... Methylation: Transfer of methyl groups from one chemical to another is called methylation. Essentially any chemical compound that has a methyl group as part of its chemical structure is capable of donating it to another chemical that needs it. The chemical that receives the methyl group is "methylat ...
1030examII
... If one strand of a DNA molecule has the base sequence of ATGTGCC the complementary strand will read: A. B. C. D. E. ...
... If one strand of a DNA molecule has the base sequence of ATGTGCC the complementary strand will read: A. B. C. D. E. ...
Wolbachia John H. Werren and Jeremy D. Bartos
... the Wolbachia outer surface protein, which could be subject to diversifying selection. Given recombination within this gene, the use of wsp for Wolbachia subgroup designation [24] should therefore be approached cautiously. Recombination has important implications for the evolutionary interactions of ...
... the Wolbachia outer surface protein, which could be subject to diversifying selection. Given recombination within this gene, the use of wsp for Wolbachia subgroup designation [24] should therefore be approached cautiously. Recombination has important implications for the evolutionary interactions of ...
Three-dimensional Structures of Bulge
... related duplex. The looped-out and stacked-in bases form an A.A reversed Hoogsteen base-pair that stacks between the surrounding base-pairs, thus stabilizing both bases participating in bulges. The double helix is frayed at one end with the two “melted” intermolecular interactions. A related structu ...
... related duplex. The looped-out and stacked-in bases form an A.A reversed Hoogsteen base-pair that stacks between the surrounding base-pairs, thus stabilizing both bases participating in bulges. The double helix is frayed at one end with the two “melted” intermolecular interactions. A related structu ...
A pseudogene cluster in the leader region of the Euglena
... -like cloverleaf structure but has changed considerably from the functional counterpart, both in terms of alternations in Invariant sites and 1n loss of base pairing 1n stem regions. These alternations rendered 1t unable to function normally, and thus this gene was identified as a pseudogene. Simila ...
... -like cloverleaf structure but has changed considerably from the functional counterpart, both in terms of alternations in Invariant sites and 1n loss of base pairing 1n stem regions. These alternations rendered 1t unable to function normally, and thus this gene was identified as a pseudogene. Simila ...
Supplementary Text S1: Detailed PCR Protocols
... suggesting PCR efficiency was independent of genomic DNA copy number. Finally, we demonstrated that omission of the control oligonucleotides had no effect on the overall accuracy of the hemi-nested TaqMan PCR using LNA probes (data not shown). To evaluate the multiplex hemi-nested PCR of IFNB1 and D ...
... suggesting PCR efficiency was independent of genomic DNA copy number. Finally, we demonstrated that omission of the control oligonucleotides had no effect on the overall accuracy of the hemi-nested TaqMan PCR using LNA probes (data not shown). To evaluate the multiplex hemi-nested PCR of IFNB1 and D ...
Analysis of Cross Sequence Similarities for Multiple - PolyU
... Most DNA-based compression algorithms rely on encoding together similar repeated regions found within one chromosome sequence. Biocompress proposed by Grumbach and Tahi (1993) is the first algorithm designed specifically for compressing DNA sequences. Both Biocompress and its second version Biocompr ...
... Most DNA-based compression algorithms rely on encoding together similar repeated regions found within one chromosome sequence. Biocompress proposed by Grumbach and Tahi (1993) is the first algorithm designed specifically for compressing DNA sequences. Both Biocompress and its second version Biocompr ...
Paper - Revision Science
... in the spaces provided • – there may bequestions more space than you need. ...
... in the spaces provided • – there may bequestions more space than you need. ...
Genome-Wide Analysis of Natural Selection on
... evolution[2,3]. Since then, various lines of evidence have confirmed the functional impact of gene regulatory mutations[4]. The majority of known human polymorphisms occur in noncoding regions, many of which are likely to underlie gene expression variation between humans[5]. Moreover, consistent wit ...
... evolution[2,3]. Since then, various lines of evidence have confirmed the functional impact of gene regulatory mutations[4]. The majority of known human polymorphisms occur in noncoding regions, many of which are likely to underlie gene expression variation between humans[5]. Moreover, consistent wit ...
Commonly Used STR Markers
... – STR – short tandem repeat – 2 to 6 bases repeated – Most commonly used for Forensics ...
... – STR – short tandem repeat – 2 to 6 bases repeated – Most commonly used for Forensics ...
The Evolutionary Reduction Principle for Linear Variation in Genetic
... organisms. In its simplest version: Transformation processes create new genetic states among offspring, and differential survival and reproduction of alternate genetic states result in the prevalence of states with the highest levels of survival and reproduction. In a more sophisticated version: evo ...
... organisms. In its simplest version: Transformation processes create new genetic states among offspring, and differential survival and reproduction of alternate genetic states result in the prevalence of states with the highest levels of survival and reproduction. In a more sophisticated version: evo ...
Separation of DNA Restriction Fragments by Ion
... (D) The fact that the two ion-exchange materials are made from the same basic support yet have differences should make possible comparisons between the relative importance ofthe basic support versus the charged substituents: Mono Q is a strong ion exchanger with a high density of quaternary amino gr ...
... (D) The fact that the two ion-exchange materials are made from the same basic support yet have differences should make possible comparisons between the relative importance ofthe basic support versus the charged substituents: Mono Q is a strong ion exchanger with a high density of quaternary amino gr ...
Diagnostic protocol for
... windows for bacterial cell wall fluorescence, looking for the cells with the size and form of Xac. This procedure permitting detection in the range of 103 cells/ml. Polymerase Chain Reaction (PCR) DNA extraction from infected citrus tissue For obtaining the more accurate PCR results, a DNA extractio ...
... windows for bacterial cell wall fluorescence, looking for the cells with the size and form of Xac. This procedure permitting detection in the range of 103 cells/ml. Polymerase Chain Reaction (PCR) DNA extraction from infected citrus tissue For obtaining the more accurate PCR results, a DNA extractio ...
Estonian HumanGenesResearchAct.
... tissue sample, the description of his or her state of health and his or her genealogy and shall deliver the personal data of the gene donor, the tissue sample, the description of his or her state of health and his or her genealogy to the chief processor or an authorised processor designated by the c ...
... tissue sample, the description of his or her state of health and his or her genealogy and shall deliver the personal data of the gene donor, the tissue sample, the description of his or her state of health and his or her genealogy to the chief processor or an authorised processor designated by the c ...
Gene Section E2F1 (E2F transcription factor 1) Atlas of Genetics and Cytogenetics
... essential components of DNA replication machinery, including DHFR, and DNA polymerase alpha, are expressed in a cell cycle dependent manner (i.e. only in late G1 and early S phase of the cell cycle). E2F1 recognizes and binds to specific DNA sequences (5'TTTSSCGS-3', where S = C/G) that lie within t ...
... essential components of DNA replication machinery, including DHFR, and DNA polymerase alpha, are expressed in a cell cycle dependent manner (i.e. only in late G1 and early S phase of the cell cycle). E2F1 recognizes and binds to specific DNA sequences (5'TTTSSCGS-3', where S = C/G) that lie within t ...
computation for chIP-seq and rNA-seq studies
... affect sensitivity and specificity, and their effects vary based on the specifics of each genome. If only uniquely mapping reads are used, some true sites of occupancy will be invisible because they are located in repeats or recent duplicated regions. Conversely, allocating lowmultiplicity multiread ...
... affect sensitivity and specificity, and their effects vary based on the specifics of each genome. If only uniquely mapping reads are used, some true sites of occupancy will be invisible because they are located in repeats or recent duplicated regions. Conversely, allocating lowmultiplicity multiread ...
Products > HT-1080 Transfection Reagent
... 5. Optional: Add 2 µl of Complex Condenser. This reagent reduces the size of transfection complex, therefore increasing transfection efficiency; however it may increase cell toxicity 6. Add prepared transfection complexes to 0.5 ml of complete growth medium with HT-1080 cells (from step 2) 7. Incuba ...
... 5. Optional: Add 2 µl of Complex Condenser. This reagent reduces the size of transfection complex, therefore increasing transfection efficiency; however it may increase cell toxicity 6. Add prepared transfection complexes to 0.5 ml of complete growth medium with HT-1080 cells (from step 2) 7. Incuba ...
Visualization, description and analysis of the Drosophila melanogaster
... A mutation is an adaptively non-directed change in the genomic sequence of an individual, and mutations in the DNA molecule are the ultimate source of genetic variation. Once a new variant appears by mutation in the DNA it can be replicated and transmitted from generation to generation. Gel electrop ...
... A mutation is an adaptively non-directed change in the genomic sequence of an individual, and mutations in the DNA molecule are the ultimate source of genetic variation. Once a new variant appears by mutation in the DNA it can be replicated and transmitted from generation to generation. Gel electrop ...
53 - Lab Times
... DNA into, or excises it from, the host’s genome. In vitro recombination of an ORF into a Gateway vector requires only the l proteins, integrase and excisionase, the E. coli protein, IHF, and specific att recombination sequences at both ends of the PCR-amplified ORF. In the first step of Gateway clon ...
... DNA into, or excises it from, the host’s genome. In vitro recombination of an ORF into a Gateway vector requires only the l proteins, integrase and excisionase, the E. coli protein, IHF, and specific att recombination sequences at both ends of the PCR-amplified ORF. In the first step of Gateway clon ...
Choosing the Right Centrifuge for Your - Laboratory
... centrifuge. For example, space may now be tighter, or the activity level of the laboratory may have increased substantially and there are now many more users. Perhaps you want to try a new protocol that you learned about at a recent conference. With so many recent advances in both science and techno ...
... centrifuge. For example, space may now be tighter, or the activity level of the laboratory may have increased substantially and there are now many more users. Perhaps you want to try a new protocol that you learned about at a recent conference. With so many recent advances in both science and techno ...
Use of lac regulatory elements for gene expression in
... Furthermore, a food-grade mutant, expressing Lactococcus lactis acetohydroxy acid synthase genes (ilvBN), was obtained with an integrative system, developed using lacG and lacF as homologous sequences for recombination. As a result, ilvBN genes were integrated in tandem between lacG and lacF in the ...
... Furthermore, a food-grade mutant, expressing Lactococcus lactis acetohydroxy acid synthase genes (ilvBN), was obtained with an integrative system, developed using lacG and lacF as homologous sequences for recombination. As a result, ilvBN genes were integrated in tandem between lacG and lacF in the ...
Cre-Lox recombination
In the field of genetics, Cre-Lox recombination is known as a site-specific recombinase technology, and is widely used to carry out deletions, insertions, translocations and inversions at specific sites in the DNA of cells. It allows the DNA modification to be targeted to a specific cell type or be triggered by a specific external stimulus. It is implemented both in eukaryotic and prokaryotic systems.The system consists of a single enzyme, Cre recombinase, that recombines a pair of short target sequences called the Lox sequences. This system can be implemented without inserting any extra supporting proteins or sequences. The Cre enzyme and the original Lox site called the LoxP sequence are derived from bacteriophage P1.Placing Lox sequences appropriately allows genes to be activated, repressed, or exchanged for other genes. At a DNA level many types of manipulations can be carried out. The activity of the Cre enzyme can be controlled so that it is expressed in a particular cell type or triggered by an external stimulus like a chemical signal or a heat shock. These targeted DNA changes are useful in cell lineage tracing and when mutants are lethal if expressed globally.The Cre-Lox system is very similar in action and in usage to the FLP-FRT recombination system.