Biology Recap
... ■ Mitosis results in two daughter cells carrying identical DNA ■ Meiosis is a two-level cell division of one diploid cell into 4 unique haploid gametes ■ DNA polymerase performs proofreading of replicated strand ■ Throughput of DNA polymerase (nucleotides / second): □ Eukaryotes: Approx. 50-100 ...
... ■ Mitosis results in two daughter cells carrying identical DNA ■ Meiosis is a two-level cell division of one diploid cell into 4 unique haploid gametes ■ DNA polymerase performs proofreading of replicated strand ■ Throughput of DNA polymerase (nucleotides / second): □ Eukaryotes: Approx. 50-100 ...
Gel Electrophoresis - Integrated DNA Technologies
... generally useful compound is N,N’-methylene bisacrylamide (bis meaning two). Polymerization of acrylamide and bisacrylamide is catalyzed in the presence of either ammonium persulphate or riboflavin. In addition, the compound N,N,N,N’tetramethylethyldiamine (TEMED) or, less commonly, 3-dimethylamino ...
... generally useful compound is N,N’-methylene bisacrylamide (bis meaning two). Polymerization of acrylamide and bisacrylamide is catalyzed in the presence of either ammonium persulphate or riboflavin. In addition, the compound N,N,N,N’tetramethylethyldiamine (TEMED) or, less commonly, 3-dimethylamino ...
Haploidization Analysis in Penicillium chrysogenum
... similar to those found by Kafer (1961) when non-disjunction and chromosome loss were postulated as causes of haploidization in Aspergillus nidulans. Mitotic crossing-over. Suspensions of conidia of the dark green (wild-type spore colour) diploid yr chorlbrr ;lysr were treated with a dose of u.v. tha ...
... similar to those found by Kafer (1961) when non-disjunction and chromosome loss were postulated as causes of haploidization in Aspergillus nidulans. Mitotic crossing-over. Suspensions of conidia of the dark green (wild-type spore colour) diploid yr chorlbrr ;lysr were treated with a dose of u.v. tha ...
Rapid Selection of Multiple Gene Integrant for the Production of
... Abstract For the rapid selection of higher recombinant hirudin producing strain in a methylotrophic yeast Hansenula polymorpha, a multiple gene integration and dose-dependent selection vector, based on a telomere-associated ARS and a bacterial aminoglycoside 3phosphotransferase (aph) gene, was adopt ...
... Abstract For the rapid selection of higher recombinant hirudin producing strain in a methylotrophic yeast Hansenula polymorpha, a multiple gene integration and dose-dependent selection vector, based on a telomere-associated ARS and a bacterial aminoglycoside 3phosphotransferase (aph) gene, was adopt ...
lecture5(GS351)
... • Copied chromosomes (sister chromatids) stay joined together at the centromere. • Homologous chromosomes pair up and physically join at sites of recombination • Proteins pull the two homologs to opposite poles Meiotic Division 2 • Proteins pull the two sister chromatids to opposite poles • Each gam ...
... • Copied chromosomes (sister chromatids) stay joined together at the centromere. • Homologous chromosomes pair up and physically join at sites of recombination • Proteins pull the two homologs to opposite poles Meiotic Division 2 • Proteins pull the two sister chromatids to opposite poles • Each gam ...
biology - Kendriya Vidyalaya No.1 Kanchrapara
... 2. Name the group of organisms and the substrate they act on, to produce biogas. 3. Which of the following is a free living bacteria that can fix nitrogen in the soil: (Spirulina, Azospirillium, Sonalika) 4. Which one of the following in the Baker’s yeast used in fermentation? (Saccharum barberi, Sa ...
... 2. Name the group of organisms and the substrate they act on, to produce biogas. 3. Which of the following is a free living bacteria that can fix nitrogen in the soil: (Spirulina, Azospirillium, Sonalika) 4. Which one of the following in the Baker’s yeast used in fermentation? (Saccharum barberi, Sa ...
A new polymerase chain reaction/restriction fragment length
... using AluI identified fragments of 10, 27, 38, 54, 106, and 135 bp for VK210 and 10, 38, and 673 bp for VK247, whereas P. vivax-like showed an unique fragment of 10 and 726 bp. In respect to RFLP, using DpnI, we observed fragments with sizes of 27, 42, 54, 81, 108, and 301 bp (VK247), whereas P. viv ...
... using AluI identified fragments of 10, 27, 38, 54, 106, and 135 bp for VK210 and 10, 38, and 673 bp for VK247, whereas P. vivax-like showed an unique fragment of 10 and 726 bp. In respect to RFLP, using DpnI, we observed fragments with sizes of 27, 42, 54, 81, 108, and 301 bp (VK247), whereas P. viv ...
Structural Studies on the Dosage Compensation Complex from
... female’s dosage compensation is prevented by the repression of MSL2. It has been shown that dosage compensation takes place in females when MSL2 is expressed ectopically. Thus Drosophila females carry all the information necessary for MSL targeting (Kelley, et al., 1995), indicating that specific se ...
... female’s dosage compensation is prevented by the repression of MSL2. It has been shown that dosage compensation takes place in females when MSL2 is expressed ectopically. Thus Drosophila females carry all the information necessary for MSL targeting (Kelley, et al., 1995), indicating that specific se ...
News from the west: Ancient DNA from a French megalithic burial
... of significant post-Neolithic migrations, they proposed a Paleolithic ancestry for modern Europeans. Nonetheless, two recent publications (Bramanti et al., 2009; Malmström et al., 2009) reported sequences from late European hunter-gatherers that demonstrated genetic differentiation between ancient h ...
... of significant post-Neolithic migrations, they proposed a Paleolithic ancestry for modern Europeans. Nonetheless, two recent publications (Bramanti et al., 2009; Malmström et al., 2009) reported sequences from late European hunter-gatherers that demonstrated genetic differentiation between ancient h ...
Eds., N. Hamamura, S. Suzuki, S. Mendo, C. M. Barroso,... © by TERRAPUB, 2010.
... problem of concern. Environmental microbes have developed some resistance mechanisms that include mercury detoxification process, therefore these mercury resistant bacteria play important roles not only in pollution issue but in the geochemical cycle of mercury in the environment. The most-studied r ...
... problem of concern. Environmental microbes have developed some resistance mechanisms that include mercury detoxification process, therefore these mercury resistant bacteria play important roles not only in pollution issue but in the geochemical cycle of mercury in the environment. The most-studied r ...
PDF
... miss genuine similarities, then functional elements could be miss-assigned as non-functional. This uncertainty largely arises due to the unknown pattern of indels (gaps) between the pair of sequences [12]. A solution to this problem is to compute probabilities of alternative alignments according to ...
... miss genuine similarities, then functional elements could be miss-assigned as non-functional. This uncertainty largely arises due to the unknown pattern of indels (gaps) between the pair of sequences [12]. A solution to this problem is to compute probabilities of alternative alignments according to ...
What is a genome?
... Many technologies can generate paired end reads (Sanger, 454, Illumina) We can use the information from pairs to order contigs into a scaffold ...
... Many technologies can generate paired end reads (Sanger, 454, Illumina) We can use the information from pairs to order contigs into a scaffold ...
Polyamines
... Spermine is a polyamine involved in cellular metabolism found in all eukaryotic cells. Formed from spermidine, it is found in a wide variety of organisms and tissues and is an essential growth factor in some bacteria. It is found as a polycation at physiological pH. Spermine is associated with nucle ...
... Spermine is a polyamine involved in cellular metabolism found in all eukaryotic cells. Formed from spermidine, it is found in a wide variety of organisms and tissues and is an essential growth factor in some bacteria. It is found as a polycation at physiological pH. Spermine is associated with nucle ...
BIO 4333/6V29: DNA Replication, Recombination, and Repair
... • Bit scores are comparable between different searches because they are normalized to account for the use of different scoring matrices and different database sizes S’ = bit score = (lS - lnK) / ln2 The E value corresponding to a given bit score is: ...
... • Bit scores are comparable between different searches because they are normalized to account for the use of different scoring matrices and different database sizes S’ = bit score = (lS - lnK) / ln2 The E value corresponding to a given bit score is: ...
types of gel - WordPress.com
... proteins. Mixtures of proteins are separated by two properties in two dimensions on 2D gels. The first dimension separates proteins according to their native isoelectric point (pI) using a form of electrophoresis called isoelectric focusing (IEF). The second dimension separates by mass using ordinar ...
... proteins. Mixtures of proteins are separated by two properties in two dimensions on 2D gels. The first dimension separates proteins according to their native isoelectric point (pI) using a form of electrophoresis called isoelectric focusing (IEF). The second dimension separates by mass using ordinar ...
Epigenetic memory in mammals
... Epigenetic information is encrypted in genetic sequences, and includes DNA methylation, histone modifications and small RNA changes (Bonasio et al., 2010). Epigenetic memory is the ability to transfer epigenetic information from one generation to the next. Epigenetic information uses patterns of inhe ...
... Epigenetic information is encrypted in genetic sequences, and includes DNA methylation, histone modifications and small RNA changes (Bonasio et al., 2010). Epigenetic memory is the ability to transfer epigenetic information from one generation to the next. Epigenetic information uses patterns of inhe ...
BLAST - UPCH
... • Bit scores are comparable between different searches because they are normalized to account for the use of different scoring matrices and different database sizes S’ = bit score = (lS - lnK) / ln2 The E value corresponding to a given bit score is: ...
... • Bit scores are comparable between different searches because they are normalized to account for the use of different scoring matrices and different database sizes S’ = bit score = (lS - lnK) / ln2 The E value corresponding to a given bit score is: ...
Erp, an extracellular protein family specific to
... plasmids were sequenced. The DNA sequences were assembled and the deduced amino acid sequences were shown to contain repeated sequences based on the PGLTS motif, as already described for the M. tuberculosis and M. leprae proteins. Fig. 1 shows a schematic representation of a multiple alignment of al ...
... plasmids were sequenced. The DNA sequences were assembled and the deduced amino acid sequences were shown to contain repeated sequences based on the PGLTS motif, as already described for the M. tuberculosis and M. leprae proteins. Fig. 1 shows a schematic representation of a multiple alignment of al ...
statistical issues in the analysis of microbial communities in soil
... a cyclical process for DNA replication and in this situation it is used to amplify the number of copies of a specific region of the 16S rDNA that is known to be highly variable between bacterial species. Denaturing gradient gel electrophoresis (DGGE) can then be applied to the peR amplified fragment ...
... a cyclical process for DNA replication and in this situation it is used to amplify the number of copies of a specific region of the 16S rDNA that is known to be highly variable between bacterial species. Denaturing gradient gel electrophoresis (DGGE) can then be applied to the peR amplified fragment ...
Jounral of Bacteriology
... ionization–time of flight mass spectrometry analysis revealed expression of the antibiotic lipopeptide products surfactin, fengycin, and bacillomycin D. The fengycin (fen) and the surfactin (srf) operons were organized and located as in B. subtilis 168. A large 37.2-kb antibiotic DNA island containi ...
... ionization–time of flight mass spectrometry analysis revealed expression of the antibiotic lipopeptide products surfactin, fengycin, and bacillomycin D. The fengycin (fen) and the surfactin (srf) operons were organized and located as in B. subtilis 168. A large 37.2-kb antibiotic DNA island containi ...
Manipulating the Plasmodium Genome
... significant matches remain only partially informative. They may reveal the biochemical activity of the product, inherent to the protein and irrespective of cellular context, for example a kinase or a phosphatase activity. They may also indicate the presence of a domain of known function, but in an o ...
... significant matches remain only partially informative. They may reveal the biochemical activity of the product, inherent to the protein and irrespective of cellular context, for example a kinase or a phosphatase activity. They may also indicate the presence of a domain of known function, but in an o ...
File
... 7. Effect of pH on Enzymes Enzymes work best within a range of pH depending on the type of enzyme. The pH that the enzymes works best at is called it’s Optimum pH If the pH is too high, the enzymes active site changes shape (denatured) What is the optimum pH of this enzyme ? ...
... 7. Effect of pH on Enzymes Enzymes work best within a range of pH depending on the type of enzyme. The pH that the enzymes works best at is called it’s Optimum pH If the pH is too high, the enzymes active site changes shape (denatured) What is the optimum pH of this enzyme ? ...
DNA Sequencing by Targeting 16S rRNA Gene for Novel Strain
... Comparison of the bacterial 16S rRNA gene sequence has emerged as a preferred genetic technique. The use of 16S rRNA gene sequences to study bacterial phylogeny and taxonomy has been by far the most common housekeeping genetic marker used for a number of reasons. These reasons include (i) its presen ...
... Comparison of the bacterial 16S rRNA gene sequence has emerged as a preferred genetic technique. The use of 16S rRNA gene sequences to study bacterial phylogeny and taxonomy has been by far the most common housekeeping genetic marker used for a number of reasons. These reasons include (i) its presen ...
Cre-Lox recombination
In the field of genetics, Cre-Lox recombination is known as a site-specific recombinase technology, and is widely used to carry out deletions, insertions, translocations and inversions at specific sites in the DNA of cells. It allows the DNA modification to be targeted to a specific cell type or be triggered by a specific external stimulus. It is implemented both in eukaryotic and prokaryotic systems.The system consists of a single enzyme, Cre recombinase, that recombines a pair of short target sequences called the Lox sequences. This system can be implemented without inserting any extra supporting proteins or sequences. The Cre enzyme and the original Lox site called the LoxP sequence are derived from bacteriophage P1.Placing Lox sequences appropriately allows genes to be activated, repressed, or exchanged for other genes. At a DNA level many types of manipulations can be carried out. The activity of the Cre enzyme can be controlled so that it is expressed in a particular cell type or triggered by an external stimulus like a chemical signal or a heat shock. These targeted DNA changes are useful in cell lineage tracing and when mutants are lethal if expressed globally.The Cre-Lox system is very similar in action and in usage to the FLP-FRT recombination system.