CYP2B6 NESTED PCR: A GOOD APPROACH FOR PATIENTS ON METHADONE Original Article
... attributed primarily to the CYP3A4, CYP2B6, and CYP2D6 proteins in humans [2,9–12]. The detection of various single nucleotide polymorphisms (SNPs) involved in methadone action in vivo could help to identify an individual’s susceptibility to methadone toxicity and improve toxicological interpretatio ...
... attributed primarily to the CYP3A4, CYP2B6, and CYP2D6 proteins in humans [2,9–12]. The detection of various single nucleotide polymorphisms (SNPs) involved in methadone action in vivo could help to identify an individual’s susceptibility to methadone toxicity and improve toxicological interpretatio ...
Unusual mutations in high functioning fragile X males
... than 200 CGGs, probably because of an impediment of the linear migration of the 40S ribosomal subunit along the 5' untranslated mRNA sequence by trinucleotide expansion. 1 Another mutational class identified in fragile X families is premutations which are smaller repeat expansions to CGG copy number ...
... than 200 CGGs, probably because of an impediment of the linear migration of the 40S ribosomal subunit along the 5' untranslated mRNA sequence by trinucleotide expansion. 1 Another mutational class identified in fragile X families is premutations which are smaller repeat expansions to CGG copy number ...
The sequence of a gene encoding convicilin from pea
... pea seeds, in addition to legumin and vicilin [1]. It can be purified from both legumin and vicilin, and it consists solely of polypeptides of Mr approx. 71 000. It does not thus contain polypeptides found in either of the two major storage proteins [2]. On the other hand, convicilin is antigenicall ...
... pea seeds, in addition to legumin and vicilin [1]. It can be purified from both legumin and vicilin, and it consists solely of polypeptides of Mr approx. 71 000. It does not thus contain polypeptides found in either of the two major storage proteins [2]. On the other hand, convicilin is antigenicall ...
chapter 17 notes
... Working with the mold Neurospora crassa, George Beadle and Edward Tatum had isolated mutants requiring arginine in their growth medium and had shown genetically that these mutants fell into three classes, each defective in a different gene. From other considerations, they suspected that the metaboli ...
... Working with the mold Neurospora crassa, George Beadle and Edward Tatum had isolated mutants requiring arginine in their growth medium and had shown genetically that these mutants fell into three classes, each defective in a different gene. From other considerations, they suspected that the metaboli ...
The hybrid origins of three perennial Medicago species
... chromosomes from root tips (2-3 cm) which were pre-treated in colchicine 0.1% for 2 h. This step ensures that the chromosomes are spread in one level/plane for easier viewing while counting, and that the spindle mechanism in the metaphase stage is arrested. In metaphase the chromosomes have been tra ...
... chromosomes from root tips (2-3 cm) which were pre-treated in colchicine 0.1% for 2 h. This step ensures that the chromosomes are spread in one level/plane for easier viewing while counting, and that the spindle mechanism in the metaphase stage is arrested. In metaphase the chromosomes have been tra ...
Chapter 11
... Basic Steps of Genetic Engineering, continued Steps in a Genetic Engineering Experiment • Genetic engineering experiments use different approaches, but most share four basic steps: Step 1 The DNA from the organism containing the gene of interest is cut by restriction enzymes. Restriction enzymes are ...
... Basic Steps of Genetic Engineering, continued Steps in a Genetic Engineering Experiment • Genetic engineering experiments use different approaches, but most share four basic steps: Step 1 The DNA from the organism containing the gene of interest is cut by restriction enzymes. Restriction enzymes are ...
- Department of Molecular Biology and Genetics, Faculty
... expression changes, that are best represented in Log2 space • Background correction; a method to determine the quality of the hybridisation either across a single microarray or between microarrays, that take this into account when measuring fluoresence intensity of the ...
... expression changes, that are best represented in Log2 space • Background correction; a method to determine the quality of the hybridisation either across a single microarray or between microarrays, that take this into account when measuring fluoresence intensity of the ...
Diagnostic protocol for
... and gently heat-fixed over a flame. Separate slides are set up for each test bacterium and also, positive and negative controls, as for ELISA. Commercially available antiserum is diluted with PBS at pH 7.2 and appropriate dilutions added to windows of each slide. Controls of normal (pre-immune) seru ...
... and gently heat-fixed over a flame. Separate slides are set up for each test bacterium and also, positive and negative controls, as for ELISA. Commercially available antiserum is diluted with PBS at pH 7.2 and appropriate dilutions added to windows of each slide. Controls of normal (pre-immune) seru ...
Cloning of the Papaya Chromoplast-Specific
... showed a strong accumulation of lycopene (approximately 63% of the total carotenoid content) in redfleshed fruit, while none was detected in yellow-fleshed fruit. The profile of yellow-fleshed fruit showed mostly b-cryptoxanthin and b-carotene derivatives, up to 75% of the total carotenoid content, ...
... showed a strong accumulation of lycopene (approximately 63% of the total carotenoid content) in redfleshed fruit, while none was detected in yellow-fleshed fruit. The profile of yellow-fleshed fruit showed mostly b-cryptoxanthin and b-carotene derivatives, up to 75% of the total carotenoid content, ...
Chapter 1: An Introduction to Chemistry
... Is red hair classified as a phenotype or a genotype? What term do we give to the carrier of the genetic information that transmits the characteristic of red hair from parent to child? ...
... Is red hair classified as a phenotype or a genotype? What term do we give to the carrier of the genetic information that transmits the characteristic of red hair from parent to child? ...
Educational Items Section Genetic Linkage Analysis Atlas of Genetics and Cytogenetics
... I- 3. Test for linkage Several methods have been proposed to detect linkage: "U scores", were suggested by Bernstein in 1931, "the sib pair test" by Penrose in 1935, "likelihood ratios" by Haldane and Smith in 1947, "the lod score method" proposed by Morton in 1955 (1). Morton’s method is the one mo ...
... I- 3. Test for linkage Several methods have been proposed to detect linkage: "U scores", were suggested by Bernstein in 1931, "the sib pair test" by Penrose in 1935, "likelihood ratios" by Haldane and Smith in 1947, "the lod score method" proposed by Morton in 1955 (1). Morton’s method is the one mo ...
Catabolic Plasmids - UQ eSpace
... cAMP-independent systems of Bacillus, acting in opposition to specific, plasmid-borne positive regulation. This is based generally on a two-component sensor/regulator ...
... cAMP-independent systems of Bacillus, acting in opposition to specific, plasmid-borne positive regulation. This is based generally on a two-component sensor/regulator ...
Ab Initio Modeling of Biological Systems - Psi-k
... However, there are many areas in which the use of effective potentials may be not appropriate, and more sophisticated and accurate approaches are required. An alternative is offered by ab initio molecular dynamics (AIMD). AIMD in the Born-Oppheneimer approximation can be efficiently performed using ...
... However, there are many areas in which the use of effective potentials may be not appropriate, and more sophisticated and accurate approaches are required. An alternative is offered by ab initio molecular dynamics (AIMD). AIMD in the Born-Oppheneimer approximation can be efficiently performed using ...
video slide - Biology at Mott
... Three properties of RNA enable it to function as an enzyme It ...
... Three properties of RNA enable it to function as an enzyme It ...
Molecular Evidence for Vector Implication of Onchocerca lupi in Los
... The nodules are primarily composed of eosinophils, histiocytes, fibroblasts, and newly formed blood vessels (Zarfoss, Dubielzig, Eberhard, & Schmidt, 2005). This is typically associated with exophthalmos and possible third-eyelid protrusion (Komnenou, Egyed, Sréter, & Eberhard, 2003). In almost all ...
... The nodules are primarily composed of eosinophils, histiocytes, fibroblasts, and newly formed blood vessels (Zarfoss, Dubielzig, Eberhard, & Schmidt, 2005). This is typically associated with exophthalmos and possible third-eyelid protrusion (Komnenou, Egyed, Sréter, & Eberhard, 2003). In almost all ...
A novel locus of Yersinia enterocolitica serotype O:3 involved in
... (YeO3-c) was sensitive to bacteriophage (I)R1-37 when grown at 37°C but not when grown at 22°C because of steric hindrance by abundant lipopolysaccharide (LPS) O-side chain (O-antigen) expressed at 22°C. The transposon library of YeO3-c was grown at 37°C and screened for phage ~R1-37-resistant trans ...
... (YeO3-c) was sensitive to bacteriophage (I)R1-37 when grown at 37°C but not when grown at 22°C because of steric hindrance by abundant lipopolysaccharide (LPS) O-side chain (O-antigen) expressed at 22°C. The transposon library of YeO3-c was grown at 37°C and screened for phage ~R1-37-resistant trans ...
Introduction The non-vascular, multicellular land plant
... have shown that the targeted disruption of a genomic locus in Physcomitrella correlates with a mutant phenotype that reveals the biological function of the disrupted gene (Girke et al., 1998; Strepp et al., 1998; Girod et al., 1999; Imaizumi et al., 2002; Koprivova et al., 2002; Lorenz et al., 2003 ...
... have shown that the targeted disruption of a genomic locus in Physcomitrella correlates with a mutant phenotype that reveals the biological function of the disrupted gene (Girke et al., 1998; Strepp et al., 1998; Girod et al., 1999; Imaizumi et al., 2002; Koprivova et al., 2002; Lorenz et al., 2003 ...
GENE EXPRESSION
... one particular molecule, it changes the shape of the protein, which changes its ability to react with a second particular molecule. Here the first molecule is the inducer allolactose and the second molecule is the operator DNA. When allolactose is bound to the repressor, it causes the repressor to ch ...
... one particular molecule, it changes the shape of the protein, which changes its ability to react with a second particular molecule. Here the first molecule is the inducer allolactose and the second molecule is the operator DNA. When allolactose is bound to the repressor, it causes the repressor to ch ...
Consistent Errors in First Strand cDNA Due to Random Hexamer
... cases. These specific mismatch patterns were observed consistently in two independent data sets (Figure 3). The distribution, type and repeatability of mismatch patterns demonstrate that not all mispriming events have the same likelihood and RNA-DNA hexamer mispriming is the main source of error in ...
... cases. These specific mismatch patterns were observed consistently in two independent data sets (Figure 3). The distribution, type and repeatability of mismatch patterns demonstrate that not all mispriming events have the same likelihood and RNA-DNA hexamer mispriming is the main source of error in ...
Chapter 18: Gene Mutation and DNA Repair
... 18.1 Effects of Mutations on Gene Structure and Function Overview The term mutation typically is associated with a negative change in the genetic material. However, detrimental or lethal mutations are actually but one class of mutations. A mutation represents any heritable change to the genetic mate ...
... 18.1 Effects of Mutations on Gene Structure and Function Overview The term mutation typically is associated with a negative change in the genetic material. However, detrimental or lethal mutations are actually but one class of mutations. A mutation represents any heritable change to the genetic mate ...
i. Genetics
... DNA stands for deoxyribonucleic acid This chemical substance is present in the nucleus of all cells in all living organisms The kind of cell which is formed, (muscle, blood, nerve etc.) is controlled by DNA The kind of organism which is produced (giraffe, herring, human, etc.) is controlled by DNA ...
... DNA stands for deoxyribonucleic acid This chemical substance is present in the nucleus of all cells in all living organisms The kind of cell which is formed, (muscle, blood, nerve etc.) is controlled by DNA The kind of organism which is produced (giraffe, herring, human, etc.) is controlled by DNA ...
Diagnostic protocol for
... and gently heat-fixed over a flame. Separate slides are set up for each test bacterium and also, positive and negative controls, as for ELISA. Commercially available antiserum is diluted with PBS at pH 7.2 and appropriate dilutions added to windows of each slide. Controls of normal (pre-immune) seru ...
... and gently heat-fixed over a flame. Separate slides are set up for each test bacterium and also, positive and negative controls, as for ELISA. Commercially available antiserum is diluted with PBS at pH 7.2 and appropriate dilutions added to windows of each slide. Controls of normal (pre-immune) seru ...
Cre-Lox recombination
In the field of genetics, Cre-Lox recombination is known as a site-specific recombinase technology, and is widely used to carry out deletions, insertions, translocations and inversions at specific sites in the DNA of cells. It allows the DNA modification to be targeted to a specific cell type or be triggered by a specific external stimulus. It is implemented both in eukaryotic and prokaryotic systems.The system consists of a single enzyme, Cre recombinase, that recombines a pair of short target sequences called the Lox sequences. This system can be implemented without inserting any extra supporting proteins or sequences. The Cre enzyme and the original Lox site called the LoxP sequence are derived from bacteriophage P1.Placing Lox sequences appropriately allows genes to be activated, repressed, or exchanged for other genes. At a DNA level many types of manipulations can be carried out. The activity of the Cre enzyme can be controlled so that it is expressed in a particular cell type or triggered by an external stimulus like a chemical signal or a heat shock. These targeted DNA changes are useful in cell lineage tracing and when mutants are lethal if expressed globally.The Cre-Lox system is very similar in action and in usage to the FLP-FRT recombination system.