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BCA Assay
... alkaline medium (the biuret reaction) with the highly sensitive and selective colorimetric detection of the cuprous cation (Cu+1) using a unique reagent containing bicinchoninic acid.1 The purple-colored reaction product of this assay is formed by the chelation of two molecules of BCA with one cupro ...
... alkaline medium (the biuret reaction) with the highly sensitive and selective colorimetric detection of the cuprous cation (Cu+1) using a unique reagent containing bicinchoninic acid.1 The purple-colored reaction product of this assay is formed by the chelation of two molecules of BCA with one cupro ...
food derived from insect-protected, glufosinate ammonium
... Food derived from genetically modified (GM) cotton line MXB-13 has been assessed for its safety for human consumption. This cotton line has been genetically modified to be resistant to insect attack and has been developed for cultivation in North America and Australia. A number of criteria have been ...
... Food derived from genetically modified (GM) cotton line MXB-13 has been assessed for its safety for human consumption. This cotton line has been genetically modified to be resistant to insect attack and has been developed for cultivation in North America and Australia. A number of criteria have been ...
attachment of amino acids to tRNA
... —the first codon of an ORF In bacteria : AUG, GUG, or UUG (5’-3’) In eukaryotic cells: 5’-AUG-3’ Functions: 1.Specifies the first amino acid to be incorporated into the growing polypeptide chain. 2.Defines the reading frame for all subsequent codons. ...
... —the first codon of an ORF In bacteria : AUG, GUG, or UUG (5’-3’) In eukaryotic cells: 5’-AUG-3’ Functions: 1.Specifies the first amino acid to be incorporated into the growing polypeptide chain. 2.Defines the reading frame for all subsequent codons. ...
Word - Food Standards Australia New Zealand
... Food derived from genetically modified (GM) cotton line MXB-13 has been assessed for its safety for human consumption. This cotton line has been genetically modified to be resistant to insect attack and has been developed for cultivation in North America and Australia. A number of criteria have been ...
... Food derived from genetically modified (GM) cotton line MXB-13 has been assessed for its safety for human consumption. This cotton line has been genetically modified to be resistant to insect attack and has been developed for cultivation in North America and Australia. A number of criteria have been ...
LEGO Lab - TeacherWeb
... Write a short paragraph that explains what does this diagram represent. Protein synthesis is the process in which the DNA directs the production of amino acids and proteins. The information stored in the molecule of DNA is transcribed into mRNA. A ribosome binds to the mRNA and tRNA translates the m ...
... Write a short paragraph that explains what does this diagram represent. Protein synthesis is the process in which the DNA directs the production of amino acids and proteins. The information stored in the molecule of DNA is transcribed into mRNA. A ribosome binds to the mRNA and tRNA translates the m ...
Structure of a Plasmodium yoelii gene
... SOOOCimmor1 of [o--32P]dATP (Amersham) by nick translation (Maniatis et al. 1982) using a kit from Boehringer and was used as a probe. Unincorporated label was removed by Sephadex G-50 column filtration (Maniatis et al. 1982). Conditions for prehybridization and hybridization were the same as those ...
... SOOOCimmor1 of [o--32P]dATP (Amersham) by nick translation (Maniatis et al. 1982) using a kit from Boehringer and was used as a probe. Unincorporated label was removed by Sephadex G-50 column filtration (Maniatis et al. 1982). Conditions for prehybridization and hybridization were the same as those ...
A Mutation in the Anticodon of a Single tRNA Is
... tRNA versus AUX/IAA mutants) the observed phenotypes are likely to be composites of downstream effects. With the mutant tRNA, we expect that a steadystate level of many mutated proteins contributes to the phenotypes, though a more direct effect or a major effect on one or more specific components ca ...
... tRNA versus AUX/IAA mutants) the observed phenotypes are likely to be composites of downstream effects. With the mutant tRNA, we expect that a steadystate level of many mutated proteins contributes to the phenotypes, though a more direct effect or a major effect on one or more specific components ca ...
Catabolic Alanine Racemase from Salmonella typhimurium: DNA Sequence, Enzyme Purification, and Characterization.
... DNA sequencing of the dadB gene in plasmid pSWl2 (Wasserman et al., 1983), this plasmid was further subcloned by using DNA manipulations as described by Davis et al. (1980) and by Maniatis et al. (1982), as illustrated in Figure 2 (top). The first step was to delete the 2.7-kb NdeI-NdeI fragment cov ...
... DNA sequencing of the dadB gene in plasmid pSWl2 (Wasserman et al., 1983), this plasmid was further subcloned by using DNA manipulations as described by Davis et al. (1980) and by Maniatis et al. (1982), as illustrated in Figure 2 (top). The first step was to delete the 2.7-kb NdeI-NdeI fragment cov ...
Expression and purification of four different rhizobial acyl carrier
... restriction site was added. The backward primer oTBC13 (5hAAAGGATCC TCA GGC CTT GGC CGC-3h) adds a BamHI restriction site. As template, genomic DNA of R. leguminosarum LPR5045 was used. Design of the primers for amplification of the acpP gene of E. coli was based on the DNA sequence of the gene (Raw ...
... restriction site was added. The backward primer oTBC13 (5hAAAGGATCC TCA GGC CTT GGC CGC-3h) adds a BamHI restriction site. As template, genomic DNA of R. leguminosarum LPR5045 was used. Design of the primers for amplification of the acpP gene of E. coli was based on the DNA sequence of the gene (Raw ...
Table S1 Genes with similar expression patterns in Qing2
... Peptidyl-prolyl cis-trans isomerase (cyclophilin) of the endoplasmic reticulum, catalyzes the cis-trans isomerization of peptide bonds N-terminal to proline residues; transcriptionally induced in response to unfolded proteins in the ER ...
... Peptidyl-prolyl cis-trans isomerase (cyclophilin) of the endoplasmic reticulum, catalyzes the cis-trans isomerization of peptide bonds N-terminal to proline residues; transcriptionally induced in response to unfolded proteins in the ER ...
The Lamin B Receptor of the Nuclear Envelope Inner Membrane: A
... integral membrane proteins that may be involved in the anchorage of these structures. In support of this notion, several integral membrane proteins associated with these structures have been identified and localized to specific nuclear envelope membrane domains. An integral membrane glycoprotein (gp ...
... integral membrane proteins that may be involved in the anchorage of these structures. In support of this notion, several integral membrane proteins associated with these structures have been identified and localized to specific nuclear envelope membrane domains. An integral membrane glycoprotein (gp ...
us whey proteins in ready-to-drink beverages
... The source of whey protein may be various whey protein concentrates (defined as having 34-89% protein), whey protein isolates (containing 90-92% protein), or whey peptides, which provide distinct nutritional and functional advantages. In some cases, whey proteins are combined with other proteins suc ...
... The source of whey protein may be various whey protein concentrates (defined as having 34-89% protein), whey protein isolates (containing 90-92% protein), or whey peptides, which provide distinct nutritional and functional advantages. In some cases, whey proteins are combined with other proteins suc ...
U.S. WHEY PROTEINS IN READY-TO-DRINK BEVERAGES
... The source of whey protein may be various whey protein concentrates (defined as having 34-89% protein), whey protein isolates (containing 90-92% protein), or whey peptides, which provide distinct nutritional and functional advantages. In some cases, whey proteins are combined with other proteins suc ...
... The source of whey protein may be various whey protein concentrates (defined as having 34-89% protein), whey protein isolates (containing 90-92% protein), or whey peptides, which provide distinct nutritional and functional advantages. In some cases, whey proteins are combined with other proteins suc ...
Sample pages 1 PDF
... and root hair elongation is inhibited (Allan et al. 2008). According to the authors, constitutive expression of MsDef1 in A. thaliana did not alter root or root hair growth. Altered expression of the tomato defensin DEF2 in transgenic tomato plants reduced pollen viability and seed production (Stotz ...
... and root hair elongation is inhibited (Allan et al. 2008). According to the authors, constitutive expression of MsDef1 in A. thaliana did not alter root or root hair growth. Altered expression of the tomato defensin DEF2 in transgenic tomato plants reduced pollen viability and seed production (Stotz ...
PDF
... determination and analysis of long DNA sequences. To analyse these data we have written a number of programs for a Cromemco Z80 based microcomputer. some of which are illustrated in Figs. 1 and 2. In this paper we would like to focus on the level of analysis that occurs prior to the running of seque ...
... determination and analysis of long DNA sequences. To analyse these data we have written a number of programs for a Cromemco Z80 based microcomputer. some of which are illustrated in Figs. 1 and 2. In this paper we would like to focus on the level of analysis that occurs prior to the running of seque ...
2013_BJ_SCFBio_Website
... Calculate resultant unit vector for each of the ORFs Classify the ORFs as genes or nongenes depending on their orientation w.r.t. universal plane (DNA space) Genes and false positives Screening of potential genes based on stereochemical properties of proteins (Protein space) Second stage screening b ...
... Calculate resultant unit vector for each of the ORFs Classify the ORFs as genes or nongenes depending on their orientation w.r.t. universal plane (DNA space) Genes and false positives Screening of potential genes based on stereochemical properties of proteins (Protein space) Second stage screening b ...
Nucleic Acids - Farmasi Unand
... as miniband units, which form the basis of the structures of chromosomes. Chromosomes are the structures that form duplicates during cell division in order to transfer the genetic information of the old cell to the two ...
... as miniband units, which form the basis of the structures of chromosomes. Chromosomes are the structures that form duplicates during cell division in order to transfer the genetic information of the old cell to the two ...
Antigenic Properties of Mycoplasma Organisms and Membranes
... in 0.25 M-NaCl to a protein concentration of 20 mg./ml. as determined by the method of Lowry, Rosebrough, Farr & Randall (195I). Thiomersalate in final concentration of I O - ~ (w/v) was added to prevent bacterial growth, and the suspension kept at 4" until used. One volume of this suspension was ad ...
... in 0.25 M-NaCl to a protein concentration of 20 mg./ml. as determined by the method of Lowry, Rosebrough, Farr & Randall (195I). Thiomersalate in final concentration of I O - ~ (w/v) was added to prevent bacterial growth, and the suspension kept at 4" until used. One volume of this suspension was ad ...
Six novel CC - DIGITAL.CSIC, el repositorio institucional
... Chemokines, a superfamily of cytokines with chemoattractant properties, are key regulators of the immune response known to act as a bridge between innate and adaptive responses, not only promoting leukocyte mobilization, but also regulating immune responses and differentiation of the recruited cells ...
... Chemokines, a superfamily of cytokines with chemoattractant properties, are key regulators of the immune response known to act as a bridge between innate and adaptive responses, not only promoting leukocyte mobilization, but also regulating immune responses and differentiation of the recruited cells ...
Biochemical and Cellular Investigation of Vitreoscilla Hemoglobin
... activity has been disclosed in VHb [25, 38]. The steadystate kinetic study has revealed that VHb catalyzes the oxidation of various aromatic substrates via a ping-pong mechanism, as normally found in horseradish peroxidase (HRP) [25]. However, this catalytic activity seems to be limited possibly as ...
... activity has been disclosed in VHb [25, 38]. The steadystate kinetic study has revealed that VHb catalyzes the oxidation of various aromatic substrates via a ping-pong mechanism, as normally found in horseradish peroxidase (HRP) [25]. However, this catalytic activity seems to be limited possibly as ...
Comparative transcriptomics reveals key gene expression
... Members of the Mycobacterium tuberculosis complex show distinct host preferences, yet the molecular basis for this tropism is unknown. Comparison of the M. tuberculosis and Mycobacterium bovis genome sequences revealed no unique genes in the bovine pathogen per se, indicating that differences in gen ...
... Members of the Mycobacterium tuberculosis complex show distinct host preferences, yet the molecular basis for this tropism is unknown. Comparison of the M. tuberculosis and Mycobacterium bovis genome sequences revealed no unique genes in the bovine pathogen per se, indicating that differences in gen ...
Syntrophic linkage between predatory Carpediemonas and
... with other microbes has rarely been investigated so far. Here, we studied an example of such an interaction by cultivating the anerobic marine flagellate, Carpediemonas frisia sp. nov. (supergroup Excavata), with parts of its naturally associated microbiome. This microbiome consisted of so far uncul ...
... with other microbes has rarely been investigated so far. Here, we studied an example of such an interaction by cultivating the anerobic marine flagellate, Carpediemonas frisia sp. nov. (supergroup Excavata), with parts of its naturally associated microbiome. This microbiome consisted of so far uncul ...
Analysis of protein carbonylation
... In the case when there are no reference materials, manufacturers of in vitro diagnostic tests prepare their ...
... In the case when there are no reference materials, manufacturers of in vitro diagnostic tests prepare their ...
... extensively studied. The glycoprotein is composed of four distinct domains: the signal peptide that allows the translocation of the polypeptide through the membrane of the endoplasmic reticulum (Tordo and Kouknetzoff, 1993), the ectodomain that is exposed on the outer surface of the virion and inclu ...
Gene expression
Gene expression is the process by which information from a gene is used in the synthesis of a functional gene product. These products are often proteins, but in non-protein coding genes such as transfer RNA (tRNA) or small nuclear RNA (snRNA) genes, the product is a functional RNA.The process of gene expression is used by all known life - eukaryotes (including multicellular organisms), prokaryotes (bacteria and archaea), and utilized by viruses - to generate the macromolecular machinery for life.Several steps in the gene expression process may be modulated, including the transcription, RNA splicing, translation, and post-translational modification of a protein. Gene regulation gives the cell control over structure and function, and is the basis for cellular differentiation, morphogenesis and the versatility and adaptability of any organism. Gene regulation may also serve as a substrate for evolutionary change, since control of the timing, location, and amount of gene expression can have a profound effect on the functions (actions) of the gene in a cell or in a multicellular organism.In genetics, gene expression is the most fundamental level at which the genotype gives rise to the phenotype, i.e. observable trait. The genetic code stored in DNA is ""interpreted"" by gene expression, and the properties of the expression give rise to the organism's phenotype. Such phenotypes are often expressed by the synthesis of proteins that control the organism's shape, or that act as enzymes catalysing specific metabolic pathways characterising the organism.