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Proteomic Analysis of Neisseria gonorrhoeae Biofilms
... A central question in these studies is how planktonic bacteria alter their gene and protein expression patterns to adapt to biofilm colony formation. In the most heavily studied biofilm organism, Pseudomonas aeruginosa [17,18,19,20], as well as in other organisms such as Escherichia coli [21,22,23,2 ...
... A central question in these studies is how planktonic bacteria alter their gene and protein expression patterns to adapt to biofilm colony formation. In the most heavily studied biofilm organism, Pseudomonas aeruginosa [17,18,19,20], as well as in other organisms such as Escherichia coli [21,22,23,2 ...
In Vivo Selection Yields AAV-B1 Capsid for Central
... comprised of 60 subunits of VP1, VP2, and VP3 capsid proteins in a ratio of ~1:1:10, and an encapsidated single-stranded DNA viral genome. In addition to protecting the genome, the capsid mediates interactions with cell surface receptors and postentry intracellular trafficking and as such, is the ma ...
... comprised of 60 subunits of VP1, VP2, and VP3 capsid proteins in a ratio of ~1:1:10, and an encapsidated single-stranded DNA viral genome. In addition to protecting the genome, the capsid mediates interactions with cell surface receptors and postentry intracellular trafficking and as such, is the ma ...
144803525 - BORA
... Tyrosine hydroxylase (TH) is the rate limiting enzyme that catalyzes the first step in the biosynthesis of catecholamines. The posttranslational modification of its regulatory domain controls the regulation of TH enzyme activity. Different kinases are responsible for the phosphorylation of the enzym ...
... Tyrosine hydroxylase (TH) is the rate limiting enzyme that catalyzes the first step in the biosynthesis of catecholamines. The posttranslational modification of its regulatory domain controls the regulation of TH enzyme activity. Different kinases are responsible for the phosphorylation of the enzym ...
Mutational analysis of exoribonuclease I from Saccharomyces
... oligonucleotide and ligated into the XhoI and SstI sites of pRS315, thereby replacing the polylinker of pRS315 and destroying the XhoI and Sst1 sites to give pAJ83. To create pRDK307 the XbaI–HindIII XRN1-containing fragment from pRDK252 (28) was ligated into the XbaI and HindIII sites of pAJ83. The ...
... oligonucleotide and ligated into the XhoI and SstI sites of pRS315, thereby replacing the polylinker of pRS315 and destroying the XhoI and Sst1 sites to give pAJ83. To create pRDK307 the XbaI–HindIII XRN1-containing fragment from pRDK252 (28) was ligated into the XbaI and HindIII sites of pAJ83. The ...
Current Microbiology
... Effect of the wild-type strain and mutants on growth of maize Inoculation of maize seedlings with P. polymyxa E681 and mutants showed effects on plant growth regarding increases in the shoot and root weights at 10 days after sowing (Table 2). The shoot and root length of bacterially treated plants w ...
... Effect of the wild-type strain and mutants on growth of maize Inoculation of maize seedlings with P. polymyxa E681 and mutants showed effects on plant growth regarding increases in the shoot and root weights at 10 days after sowing (Table 2). The shoot and root length of bacterially treated plants w ...
PowerPoint Presentation - Secondary structure prediction
... • This kind of consensus approach can be applied to both the template selection and the pairwise alignment between the target and template • We have also shown in class that a consensus approach can be applied to predicted structures for numerous homologs in a family related to the target ...
... • This kind of consensus approach can be applied to both the template selection and the pairwise alignment between the target and template • We have also shown in class that a consensus approach can be applied to predicted structures for numerous homologs in a family related to the target ...
PrionPPSatBlack
... among the various organisms examined. Investigating how amino acid sequence may be linked to the overall structure of the protein Examining the role of repetitive elements in prion homologies. ...
... among the various organisms examined. Investigating how amino acid sequence may be linked to the overall structure of the protein Examining the role of repetitive elements in prion homologies. ...
NIH Public Access
... colony raised from an LB/carbenicillin agar plate was used to inoculate a starter culture of 100 mL PG medium. After growing overnight at 37 °C, 15 mL of starter culture was used to inoculate each 500 mL growth flask, which was then allowed to grow at 37 °C to an optical cell density of 0.8 OD600. F ...
... colony raised from an LB/carbenicillin agar plate was used to inoculate a starter culture of 100 mL PG medium. After growing overnight at 37 °C, 15 mL of starter culture was used to inoculate each 500 mL growth flask, which was then allowed to grow at 37 °C to an optical cell density of 0.8 OD600. F ...
Ribozyme catalysis: not different, just worse
... there are only a limited number of mechanisms through which a reaction can be catalyzed. The overall goal is to stabilize the transition state of the reaction relative to the ground state. To do this, the catalyst can pay for the cost of entropy lost in achieving the transition state by positioning ...
... there are only a limited number of mechanisms through which a reaction can be catalyzed. The overall goal is to stabilize the transition state of the reaction relative to the ground state. To do this, the catalyst can pay for the cost of entropy lost in achieving the transition state by positioning ...
Pierce BCA Protein Assay Kit
... by protein in an alkaline medium (the biuret reaction) with the highly sensitive and selective colorimetric detection of the cuprous cation (Cu+1) using a unique reagent containing bicinchoninic acid.1 The purple-colored reaction product of this assay is formed by the chelation of two molecules of B ...
... by protein in an alkaline medium (the biuret reaction) with the highly sensitive and selective colorimetric detection of the cuprous cation (Cu+1) using a unique reagent containing bicinchoninic acid.1 The purple-colored reaction product of this assay is formed by the chelation of two molecules of B ...
Supplements - Haiyuan Yu
... protein. Loci are converted by separating the coding sequence of the transcript into codons, and aligning amino acids 1–n in the protein directly to codons 1–n in the transcript. All possible single-nucleotide variants in the transcript codon (3 possible alternate nucleotides × 3 possible positions ...
... protein. Loci are converted by separating the coding sequence of the transcript into codons, and aligning amino acids 1–n in the protein directly to codons 1–n in the transcript. All possible single-nucleotide variants in the transcript codon (3 possible alternate nucleotides × 3 possible positions ...
Sequence, expression, and characterization of the first archaeal ATP
... phosphoryl acceptors: in addition to F-6-P, glucose 6-phosphate, adenosine, fructose, ribose 5-phosphate, and ribose were accepted. Enzyme activity required divalent cations; Mg2+, which was most effective, could partially be replaced by Co2+, Ni2+, or Mn2+. The enzyme had a temperature optimum of 9 ...
... phosphoryl acceptors: in addition to F-6-P, glucose 6-phosphate, adenosine, fructose, ribose 5-phosphate, and ribose were accepted. Enzyme activity required divalent cations; Mg2+, which was most effective, could partially be replaced by Co2+, Ni2+, or Mn2+. The enzyme had a temperature optimum of 9 ...
Identification of the tRNA-binding Protein Arc1p as a Novel Target of
... Yeast Strains, Vectors, and Media—The S. cerevisiae strains used in this study are listed in Table I. For recombinant plasmid constructions, the S. cerevisiae/E. coli shuttle vectors YCplac33 (14), p414MET25 (15), pVT-100U (16), pTRC-HISA (Invitrogen) and the E. coli plasmid pQE70 (Qiagen) served as ...
... Yeast Strains, Vectors, and Media—The S. cerevisiae strains used in this study are listed in Table I. For recombinant plasmid constructions, the S. cerevisiae/E. coli shuttle vectors YCplac33 (14), p414MET25 (15), pVT-100U (16), pTRC-HISA (Invitrogen) and the E. coli plasmid pQE70 (Qiagen) served as ...
ANSWER: Amino acids
... therefore function of the protein Primary: order of specific amino acids Secondary: formation of helices or pleated sheets due to H bonding between O or N atoms in localized areas Tertiary: overall 3D shape of the polypeptide Quaternary: forms when 2 or more polypeptides ...
... therefore function of the protein Primary: order of specific amino acids Secondary: formation of helices or pleated sheets due to H bonding between O or N atoms in localized areas Tertiary: overall 3D shape of the polypeptide Quaternary: forms when 2 or more polypeptides ...
types of gel - WordPress.com
... Agarose gels are made from the natural polysaccharide polymers extracted from seaweed. Agarose gels are easily cast and handled compared to other matrices, because the gel setting is a physical rather than chemical change. Samples are also easily recovered. After the experiment is finished, the resu ...
... Agarose gels are made from the natural polysaccharide polymers extracted from seaweed. Agarose gels are easily cast and handled compared to other matrices, because the gel setting is a physical rather than chemical change. Samples are also easily recovered. After the experiment is finished, the resu ...
Nucleic Acids
... the antiparallel strand must be thymine. Similarly, if the purine in one strand is guanine, its complement in the antiparallel strand must be cytosine. A significant feature of Watson and Crick’s model is that no other base pairing is consistent with the observed thickness of a DNA molecule. A pair o ...
... the antiparallel strand must be thymine. Similarly, if the purine in one strand is guanine, its complement in the antiparallel strand must be cytosine. A significant feature of Watson and Crick’s model is that no other base pairing is consistent with the observed thickness of a DNA molecule. A pair o ...
Nucleotide Sequence of the Gene Coding for the
... icant domainsor regions inthe nitrogenase proteins which are the atmosphere. Nitrogenase catalyzes the overall reaction: not conserved, and are these regions responsible for the obN2 3H2+ 2NH3; the detailsof its mechanism are notknown served differences in the nitrogenase proteins? If this is true, ...
... icant domainsor regions inthe nitrogenase proteins which are the atmosphere. Nitrogenase catalyzes the overall reaction: not conserved, and are these regions responsible for the obN2 3H2+ 2NH3; the detailsof its mechanism are notknown served differences in the nitrogenase proteins? If this is true, ...
Molecular Characterization of CDC42, a Saccharomyces cerevisiae
... amino acids overall) were most pronounced in the regions that have been implicated in GTP binding and hydrolysis and in the COOH-terminal modifications leading to membrane association, suggesting that CDC42 function also involves these biochemical properties. The similarities to the rho proteins (~6 ...
... amino acids overall) were most pronounced in the regions that have been implicated in GTP binding and hydrolysis and in the COOH-terminal modifications leading to membrane association, suggesting that CDC42 function also involves these biochemical properties. The similarities to the rho proteins (~6 ...
The use of glycogen and GlycoBlue reagent in Qubit DNA and RNA
... Figure 8. Results from the Qubit RNA HS assay for samples containing RNA plus 25 ng/μL glycogen or GlycoBlue Coprecipitant (in the assay tube) compared to samples containing RNA only. The RNA concentration in the assay tubes was 500 ng/mL (near the high end of the Qubit RNA HS assay range). Note tha ...
... Figure 8. Results from the Qubit RNA HS assay for samples containing RNA plus 25 ng/μL glycogen or GlycoBlue Coprecipitant (in the assay tube) compared to samples containing RNA only. The RNA concentration in the assay tubes was 500 ng/mL (near the high end of the Qubit RNA HS assay range). Note tha ...
Lecture 2
... adjacent strand through hydrogen bonds. Amino acids in anti-parallel strands on the other hand interact with only one amino acid on an adjacent strand. Protein secondary structures – Turns & Loops: Almost all proteins exhibit a compact, globular structure which is possible only if there are turns or ...
... adjacent strand through hydrogen bonds. Amino acids in anti-parallel strands on the other hand interact with only one amino acid on an adjacent strand. Protein secondary structures – Turns & Loops: Almost all proteins exhibit a compact, globular structure which is possible only if there are turns or ...
Intrinsic Disorder in Cell-signaling and Cancer
... mostly non-redundant control for estimating the false-positive disorder prediction error rate. (b) Percentages of residues in the four datasets predicted to be disordered within segments of length $ the value on the x-axis. ...
... mostly non-redundant control for estimating the false-positive disorder prediction error rate. (b) Percentages of residues in the four datasets predicted to be disordered within segments of length $ the value on the x-axis. ...
Proteomics Analysis of Immunoprecipitated Proteins Associated with
... was shown that Tpl-2, the rat homologue of Cot, was required only for ERK and not JNK or p38 activation in LPS-stimulated macrophages (Dumitru et al., 2000). The oncogenic form of Cot contains a C-terminal truncation where the last 69 amino acids of the wild-type protein are replaced with an unrelat ...
... was shown that Tpl-2, the rat homologue of Cot, was required only for ERK and not JNK or p38 activation in LPS-stimulated macrophages (Dumitru et al., 2000). The oncogenic form of Cot contains a C-terminal truncation where the last 69 amino acids of the wild-type protein are replaced with an unrelat ...
AP Biology Concept Outline
... Evolution is a change in the genetic makeup of a population over time, with natural selection its major driving mechanism. Darwin’s theory, which is supported by evidence from many scientific disciplines, states that inheritable variations occur in individuals in a population. Due to competition for ...
... Evolution is a change in the genetic makeup of a population over time, with natural selection its major driving mechanism. Darwin’s theory, which is supported by evidence from many scientific disciplines, states that inheritable variations occur in individuals in a population. Due to competition for ...
Inhibition of the p53 tumor suppressor by MDM2
... ligase, this means it binds to its target (p53 protein) through an E3 enzyme complex due to transcriptional recognition on the N-terminal domain on the polypeptide chain. In essence, the ubiquitin proteins are loaded into the E3 complex to signal protein degradtion in order to deactivate the transc ...
... ligase, this means it binds to its target (p53 protein) through an E3 enzyme complex due to transcriptional recognition on the N-terminal domain on the polypeptide chain. In essence, the ubiquitin proteins are loaded into the E3 complex to signal protein degradtion in order to deactivate the transc ...
Optical Tweezers: Measuring Piconewton Forces
... objective is always equal to or greater than the input aperture of the objective. Thus, light that is deflected by the trapped bead is not collected by the objective. However, a dual-beam instrument can sacrifice some trapping force and only fill half of the objective input aperture, as shown in Fig ...
... objective is always equal to or greater than the input aperture of the objective. Thus, light that is deflected by the trapped bead is not collected by the objective. However, a dual-beam instrument can sacrifice some trapping force and only fill half of the objective input aperture, as shown in Fig ...
Gene expression
Gene expression is the process by which information from a gene is used in the synthesis of a functional gene product. These products are often proteins, but in non-protein coding genes such as transfer RNA (tRNA) or small nuclear RNA (snRNA) genes, the product is a functional RNA.The process of gene expression is used by all known life - eukaryotes (including multicellular organisms), prokaryotes (bacteria and archaea), and utilized by viruses - to generate the macromolecular machinery for life.Several steps in the gene expression process may be modulated, including the transcription, RNA splicing, translation, and post-translational modification of a protein. Gene regulation gives the cell control over structure and function, and is the basis for cellular differentiation, morphogenesis and the versatility and adaptability of any organism. Gene regulation may also serve as a substrate for evolutionary change, since control of the timing, location, and amount of gene expression can have a profound effect on the functions (actions) of the gene in a cell or in a multicellular organism.In genetics, gene expression is the most fundamental level at which the genotype gives rise to the phenotype, i.e. observable trait. The genetic code stored in DNA is ""interpreted"" by gene expression, and the properties of the expression give rise to the organism's phenotype. Such phenotypes are often expressed by the synthesis of proteins that control the organism's shape, or that act as enzymes catalysing specific metabolic pathways characterising the organism.