Effect of Six Decades of Selective Breeding on
... To evaluate the extent of the genetic change and its effects on the seed protein composition of soybean cultivars released during the past 60 years, representative ancestral cultivars and those derived from selective breeding were grown in a side-by-side comparison. Total seed protein content, deter ...
... To evaluate the extent of the genetic change and its effects on the seed protein composition of soybean cultivars released during the past 60 years, representative ancestral cultivars and those derived from selective breeding were grown in a side-by-side comparison. Total seed protein content, deter ...
Multiple linear regression for protein secondary structure prediction
... Prediction of the three-dimensional structure of a protein from its sequence is becoming a pressing problem for many biologists because the discrepancy continues to increase between the number of known protein sequences and the number of experimentally determined structures. The prediction of second ...
... Prediction of the three-dimensional structure of a protein from its sequence is becoming a pressing problem for many biologists because the discrepancy continues to increase between the number of known protein sequences and the number of experimentally determined structures. The prediction of second ...
Identifying Importance of Amino Acids for Protein
... Due to the difficulties and cost of actual experimental studies, it is important to develop rapid computational tools to identify the folding kinetics of a given protein from its crystal structure. The ultimate goal is to be able to predict the protein-folding kinetics of a given protein from its se ...
... Due to the difficulties and cost of actual experimental studies, it is important to develop rapid computational tools to identify the folding kinetics of a given protein from its crystal structure. The ultimate goal is to be able to predict the protein-folding kinetics of a given protein from its se ...
1 BIOINFORMATICS Bioinformatics, based on National Institutes of
... REFERENCE: The sequence seems reliable since there is high number of reference (it was published earlier by many). Features: Source: 1..9179 (number of bases) /organism="Homo sapiens" /mol_type="mRNA" /db_xref="taxon:9606" (taxonomy database: “The NCBI taxonomy database contains the names of all org ...
... REFERENCE: The sequence seems reliable since there is high number of reference (it was published earlier by many). Features: Source: 1..9179 (number of bases) /organism="Homo sapiens" /mol_type="mRNA" /db_xref="taxon:9606" (taxonomy database: “The NCBI taxonomy database contains the names of all org ...
AtPex14p maintains peroxisomal functions by determining protein
... transformation of plant peroxisomes is controlled by gene expression, protein translocation and protein degradation, although the detailed mechanisms underlying these processes still need to be clari®ed (Nishimura et al., 1996). To identify the genes responsible for regulation of peroxisomal functio ...
... transformation of plant peroxisomes is controlled by gene expression, protein translocation and protein degradation, although the detailed mechanisms underlying these processes still need to be clari®ed (Nishimura et al., 1996). To identify the genes responsible for regulation of peroxisomal functio ...
Modeling tRNA*s Translator Function
... • Go to the tRNA activity page at http://pdb101.rcsb.org/learn/resource/trna-activity-page or search for “Paper Model of tRNA – RCSB Protein Data Bank”. • Scroll down to section #3 titled, “Explore Atomic Structure of tRNA” and select the “Model color scheme” option for coloring the interactive mode ...
... • Go to the tRNA activity page at http://pdb101.rcsb.org/learn/resource/trna-activity-page or search for “Paper Model of tRNA – RCSB Protein Data Bank”. • Scroll down to section #3 titled, “Explore Atomic Structure of tRNA” and select the “Model color scheme” option for coloring the interactive mode ...
Genetic and biochemical identification of the
... State Key Laboratory of Microbial Resources, Institute of Microbiology, Chinese Academy of Sciences, Beijing 100101, PR China Environmental Microbiology and Biotechnology Research Center, Institute of Microbiology, Chinese Academy of Sciences, Beijing 100101, PR China ...
... State Key Laboratory of Microbial Resources, Institute of Microbiology, Chinese Academy of Sciences, Beijing 100101, PR China Environmental Microbiology and Biotechnology Research Center, Institute of Microbiology, Chinese Academy of Sciences, Beijing 100101, PR China ...
`Hybrid Protein Model`for optimally defining 3D protein structure
... distances, consensus or crystallographic approaches (Colloc’h et al., 1993; Frishman and Argos, 1995; Labesse et al., 1997; King and Johnson, 1999). The α-helix and βsheet repetitive structures represent less than 50% of all protein folds. Attempts to classify coils have not yielded completely satis ...
... distances, consensus or crystallographic approaches (Colloc’h et al., 1993; Frishman and Argos, 1995; Labesse et al., 1997; King and Johnson, 1999). The α-helix and βsheet repetitive structures represent less than 50% of all protein folds. Attempts to classify coils have not yielded completely satis ...
Molecular insights into mitochondrial transcription and its
... continued degradation takes place. In the matrix fatty acids go through a series of oxidation, hydration and thiolysis reactions to form acetyl-CoA and a CoAconjugated, two carbon atoms shorter, fatty acid. The shortened fatty acid can go through a new round of oxidation whereas the acetyl-CoA can e ...
... continued degradation takes place. In the matrix fatty acids go through a series of oxidation, hydration and thiolysis reactions to form acetyl-CoA and a CoAconjugated, two carbon atoms shorter, fatty acid. The shortened fatty acid can go through a new round of oxidation whereas the acetyl-CoA can e ...
Statistical analysis of DNA microarray data
... Six basic environment classes (E, P1, P2, B1, B2 and B3) The environment of each residue in the three-dimensional structure is first classified according to the area of the side chain that is buried in the protein. ---- A residue is considered exposed to solvent (environment class E) if the area ...
... Six basic environment classes (E, P1, P2, B1, B2 and B3) The environment of each residue in the three-dimensional structure is first classified according to the area of the side chain that is buried in the protein. ---- A residue is considered exposed to solvent (environment class E) if the area ...
Expression of vascular endothelial growth factor
... al., 1989; Conn et al., 1990a). This factor has also been termed vascular permeability factor (VPF) because of its ability to induce vascular leakage in vivo (Senger et al., 1983; Keck et al., 1989). Other effects of VEGF include the induction of the procoagulant activity of monocytes and endothelia ...
... al., 1989; Conn et al., 1990a). This factor has also been termed vascular permeability factor (VPF) because of its ability to induce vascular leakage in vivo (Senger et al., 1983; Keck et al., 1989). Other effects of VEGF include the induction of the procoagulant activity of monocytes and endothelia ...
An Efficient Protocol for Identifying Separation-of-Function
... that might alleviate the two obstacles described above. Our experimental system was the EST3 gene, which encodes a small (181 amino acid) subunit of yeast telomerase with two distinct categories of sof2 alleles and loss-of-function (LOF) as well as overexpression dominant-negative (ODN) phenotypes t ...
... that might alleviate the two obstacles described above. Our experimental system was the EST3 gene, which encodes a small (181 amino acid) subunit of yeast telomerase with two distinct categories of sof2 alleles and loss-of-function (LOF) as well as overexpression dominant-negative (ODN) phenotypes t ...
HHMI meeting, FOLDING
... for many proteins, the main question as to how the protein chain can find its native structure among zillions of alternatives remained unanswered. A progress in the understanding was achieved when studies involved small proteins (of 50 - 100 residues). Many of them are “two-state folders”: they fold ...
... for many proteins, the main question as to how the protein chain can find its native structure among zillions of alternatives remained unanswered. A progress in the understanding was achieved when studies involved small proteins (of 50 - 100 residues). Many of them are “two-state folders”: they fold ...
intact-protein based sample preparation strategies for
... range of protein abundance present a formidable challenge for analysis that no one analytical technique can overcome. As a result, there is a need to integrate technologies to achieve the high-resolution and high-sensitivity analysis of complex biological samples. The combined technologies of separa ...
... range of protein abundance present a formidable challenge for analysis that no one analytical technique can overcome. As a result, there is a need to integrate technologies to achieve the high-resolution and high-sensitivity analysis of complex biological samples. The combined technologies of separa ...
Predicting protein folding rates from geometric contact and amino
... San Francisco). We have slightly modified this data set and have removed structures that contain large hetero groups, such as iron protoporphyrins, or irregular amino acids. We have also incorporated additional data from the literature. These proteins belong to different structural classes: 18 are a ...
... San Francisco). We have slightly modified this data set and have removed structures that contain large hetero groups, such as iron protoporphyrins, or irregular amino acids. We have also incorporated additional data from the literature. These proteins belong to different structural classes: 18 are a ...
The 92-kDa chitinase from Streptomyces olivaceoviridis contains a
... proteinases by FPLC from the protein concentrate in the presence of Pefabloc SC. The proteins were blotted and the N-terminal amino acid sequence determined. As can be seen from Table 1, the amino acid sequences of the three proteinases are identical. For comparison the Nterminal sequence of 92-kDa ...
... proteinases by FPLC from the protein concentrate in the presence of Pefabloc SC. The proteins were blotted and the N-terminal amino acid sequence determined. As can be seen from Table 1, the amino acid sequences of the three proteinases are identical. For comparison the Nterminal sequence of 92-kDa ...
The complete nucleotide sequence of the RNA coding for the
... genome of approximately 8,500 nucleotides. The genome is 3' polyadenylated, can serve directly as a messenger and contains a poly (C) tract (100-200 nucleotides long) located approximately 400 nucleotides from the 5' end (1). Unlike most eukaryotlc mRNAs, FMDV RNA is not capped at its 51 end but has ...
... genome of approximately 8,500 nucleotides. The genome is 3' polyadenylated, can serve directly as a messenger and contains a poly (C) tract (100-200 nucleotides long) located approximately 400 nucleotides from the 5' end (1). Unlike most eukaryotlc mRNAs, FMDV RNA is not capped at its 51 end but has ...
Polymer scaling laws of unfolded and intrinsically disordered
... physics (7–9) have frequently been used to describe the properties of unfolded polypeptide chains (4, 10, 11) with the goal to establish the link between protein folding and collapse (12–15). However, the methodology to test many of these concepts experimentally has only become available rather rece ...
... physics (7–9) have frequently been used to describe the properties of unfolded polypeptide chains (4, 10, 11) with the goal to establish the link between protein folding and collapse (12–15). However, the methodology to test many of these concepts experimentally has only become available rather rece ...
Inducers of Plant Systemic Acquired Resistance Regulate NPR1
... when treated with DTT causing monomerization revealing endogenous and GFP tagged ...
... when treated with DTT causing monomerization revealing endogenous and GFP tagged ...
PDF
... physics (7–9) have frequently been used to describe the properties of unfolded polypeptide chains (4, 10, 11) with the goal to establish the link between protein folding and collapse (12–15). However, the methodology to test many of these concepts experimentally has only become available rather rece ...
... physics (7–9) have frequently been used to describe the properties of unfolded polypeptide chains (4, 10, 11) with the goal to establish the link between protein folding and collapse (12–15). However, the methodology to test many of these concepts experimentally has only become available rather rece ...
Arabidopsis Genes Encoding Components of the
... represent just the last six exons of AtTOC75-III. In fact, this gene seems to be an extreme case of a more common phenomenon. For a few components, including Toc75 and Toc159, BLAST searches reveal several small regions with high levels of sequence similarity to these subunits throughout the genome. ...
... represent just the last six exons of AtTOC75-III. In fact, this gene seems to be an extreme case of a more common phenomenon. For a few components, including Toc75 and Toc159, BLAST searches reveal several small regions with high levels of sequence similarity to these subunits throughout the genome. ...
Small-angle scattering studies of intrinsically disordered proteins
... proteins. In order to fully exploit the structural and dynamic information encoded in SAS data, it is necessary to use realistic three-dimensional (3D) models. However, the generation of conformational ensembles of disordered proteins is extremely challenging, mainly because of the flat energy lands ...
... proteins. In order to fully exploit the structural and dynamic information encoded in SAS data, it is necessary to use realistic three-dimensional (3D) models. However, the generation of conformational ensembles of disordered proteins is extremely challenging, mainly because of the flat energy lands ...
Although the administration of testosterone clearly causes marked
... described by Bray (14). In some experiments free tyrosine was determined on another portion of the supernatant by the spectrophotofluorometric method of Waalkes and Udenfriend (15). In the experiments utilizing acetate2-C14 as substrate the perchloric acid supernatant was adjusted to pH 7, diluted t ...
... described by Bray (14). In some experiments free tyrosine was determined on another portion of the supernatant by the spectrophotofluorometric method of Waalkes and Udenfriend (15). In the experiments utilizing acetate2-C14 as substrate the perchloric acid supernatant was adjusted to pH 7, diluted t ...
Chapter 5 - Red Hook Central Schools
... Proteins have many structures, resulting in a wide range of functions • Proteins account for more than 50% of the dry mass of most cells. • Protein functions include structural support, storage, transport, cellular communications, movement, defense against foreign substances, and organic catalysts ...
... Proteins have many structures, resulting in a wide range of functions • Proteins account for more than 50% of the dry mass of most cells. • Protein functions include structural support, storage, transport, cellular communications, movement, defense against foreign substances, and organic catalysts ...
ELECTROPHORESIS
... Commonly used buffers are tris acetate, tris borate or tris phosphate at a concentration of 50mM over a pH range of 7.5-8.0. ...
... Commonly used buffers are tris acetate, tris borate or tris phosphate at a concentration of 50mM over a pH range of 7.5-8.0. ...
Gene expression
Gene expression is the process by which information from a gene is used in the synthesis of a functional gene product. These products are often proteins, but in non-protein coding genes such as transfer RNA (tRNA) or small nuclear RNA (snRNA) genes, the product is a functional RNA.The process of gene expression is used by all known life - eukaryotes (including multicellular organisms), prokaryotes (bacteria and archaea), and utilized by viruses - to generate the macromolecular machinery for life.Several steps in the gene expression process may be modulated, including the transcription, RNA splicing, translation, and post-translational modification of a protein. Gene regulation gives the cell control over structure and function, and is the basis for cellular differentiation, morphogenesis and the versatility and adaptability of any organism. Gene regulation may also serve as a substrate for evolutionary change, since control of the timing, location, and amount of gene expression can have a profound effect on the functions (actions) of the gene in a cell or in a multicellular organism.In genetics, gene expression is the most fundamental level at which the genotype gives rise to the phenotype, i.e. observable trait. The genetic code stored in DNA is ""interpreted"" by gene expression, and the properties of the expression give rise to the organism's phenotype. Such phenotypes are often expressed by the synthesis of proteins that control the organism's shape, or that act as enzymes catalysing specific metabolic pathways characterising the organism.