Type and timing of protein feeding to optimize anabolism
... a marked inhibition of protein degradation. It is possible that the stimulation of protein synthesis does not occur when the amounts of protein fed with small meals are low. In the early post-absorptive state, free amino acid levels decrease with time when the last meal becomes more and more distant ...
... a marked inhibition of protein degradation. It is possible that the stimulation of protein synthesis does not occur when the amounts of protein fed with small meals are low. In the early post-absorptive state, free amino acid levels decrease with time when the last meal becomes more and more distant ...
Protein Foods
... o Activity level: Untrained athletes, or individuals going from no activity to moderate or high levels of physical activity, have a period of higher protein needs. The initiation of endurance training also increases protein needs for a time. o Energy and carbohydrate adequacy: When adequate energy i ...
... o Activity level: Untrained athletes, or individuals going from no activity to moderate or high levels of physical activity, have a period of higher protein needs. The initiation of endurance training also increases protein needs for a time. o Energy and carbohydrate adequacy: When adequate energy i ...
NMR Analysis of Protein
... NMR Analysis of Protein-Ligand Interactions Protein Chemical Shift Changes Upon Ligand Binding • Assigned 2D 1H-15N HSQC NMR Spectra overlay spectra in presence/absence of ligand changes in peak position indicate binding identity of peaks that change identifies binding site on protein surface ...
... NMR Analysis of Protein-Ligand Interactions Protein Chemical Shift Changes Upon Ligand Binding • Assigned 2D 1H-15N HSQC NMR Spectra overlay spectra in presence/absence of ligand changes in peak position indicate binding identity of peaks that change identifies binding site on protein surface ...
Pharmacophore screening of the Protein Data Bank for specific
... searching the PDB for pockets lined by clusters of aromatic and acidic residues. For each PDB structure, the protein is first stripped of water molecules, ligands (including nucleic acids) and bound peptides (any chain shorter than 25 residues is removed), converted to an ICM object (using the makeB ...
... searching the PDB for pockets lined by clusters of aromatic and acidic residues. For each PDB structure, the protein is first stripped of water molecules, ligands (including nucleic acids) and bound peptides (any chain shorter than 25 residues is removed), converted to an ICM object (using the makeB ...
Protein kinases of the human malaria parasite Plasmodium
... fifth CDPK [PF13_0211], which like the four cited above possesses four EF-hand motifs, has been discovered in the present study. PF11_0242 appears to be related to CDPKs, but contains only one EF-hand motif. PfPK2 [Pfl1885c] constitutes a sister branch to the CDPK group. This enzyme was previously c ...
... fifth CDPK [PF13_0211], which like the four cited above possesses four EF-hand motifs, has been discovered in the present study. PF11_0242 appears to be related to CDPKs, but contains only one EF-hand motif. PfPK2 [Pfl1885c] constitutes a sister branch to the CDPK group. This enzyme was previously c ...
Gene encoding the group B streptococcal protein R4, its
... cent pepsin5, 7 and characterization of various R proteins has continued in several laboratories6-8. The R4 protein which has been well characterized in our laboratory, conforms to these criteria, is found predominantly in type III as well as some type II and V invasive isolates that cause disease, ...
... cent pepsin5, 7 and characterization of various R proteins has continued in several laboratories6-8. The R4 protein which has been well characterized in our laboratory, conforms to these criteria, is found predominantly in type III as well as some type II and V invasive isolates that cause disease, ...
Data Correlation Details: Enrichment Analysis
... be seen by looking at a single, existing experiment. These results may be interesting in themselves (eg, associations between different treatment factors, or between a treatment and an existing known pathway or protein family), or may be used to guide further research and experimentation.1,2 The pri ...
... be seen by looking at a single, existing experiment. These results may be interesting in themselves (eg, associations between different treatment factors, or between a treatment and an existing known pathway or protein family), or may be used to guide further research and experimentation.1,2 The pri ...
Circadian Rhythm of Intestinal SucraseActivity in Rats
... done at 4°C. The animals were stunned, decapitated, and the abdominal cavity opened with prompt removal of the small intestine from the ligament of Treitz to the cecum. The intestine was rinsed with normal saline, flushed with saline, everted, rinsed again, and then scraped over ice with a glass sli ...
... done at 4°C. The animals were stunned, decapitated, and the abdominal cavity opened with prompt removal of the small intestine from the ligament of Treitz to the cecum. The intestine was rinsed with normal saline, flushed with saline, everted, rinsed again, and then scraped over ice with a glass sli ...
Genomics Meets Phylogenetics
... Information, Bethesda, MD) searches or other intergenomic comparisons. Phenetic comparisons have the advantage of computational efficiency. Most such methods are algorithms for constructing a single tree rather than evaluating a large ensemble of possible topologies, using an optimality criterion, s ...
... Information, Bethesda, MD) searches or other intergenomic comparisons. Phenetic comparisons have the advantage of computational efficiency. Most such methods are algorithms for constructing a single tree rather than evaluating a large ensemble of possible topologies, using an optimality criterion, s ...
An LL-Diaminopimelate Aminotransferase
... was assessed to further evaluate whether it is possible that such an enzyme is generally involved in Lys biosynthesis in plants and their photosynthetic allies. Extracts prepared from a variety of vascular plants, from a moss, a green alga, and a cyanobacteria all showed LL-DAP-AT activity, whereas ...
... was assessed to further evaluate whether it is possible that such an enzyme is generally involved in Lys biosynthesis in plants and their photosynthetic allies. Extracts prepared from a variety of vascular plants, from a moss, a green alga, and a cyanobacteria all showed LL-DAP-AT activity, whereas ...
Khoa hoc - Cong nghe
... coincides with research of scad hydrolysis by Flavourzyme (Chun et al., 2006). A number of other studies on hydrolyzed tuna head (Nguyen et al., 2012) and hydrolysis salmon head (Sathivel et al., 2005) also showed that essential amino acids content in hydrolyzed protein obtained from these studied w ...
... coincides with research of scad hydrolysis by Flavourzyme (Chun et al., 2006). A number of other studies on hydrolyzed tuna head (Nguyen et al., 2012) and hydrolysis salmon head (Sathivel et al., 2005) also showed that essential amino acids content in hydrolyzed protein obtained from these studied w ...
REPLI-g WTA Single Cell Handbook
... Different protocols in this handbook provide detailed instructions for using the REPLI-g WTA Single Cell Kit for cDNA amplification of single cells or purified RNA. Use Table 2 (page 15) to select the appropriate protocol based on starting material and downstream analysis. The protocol “Amplificatio ...
... Different protocols in this handbook provide detailed instructions for using the REPLI-g WTA Single Cell Kit for cDNA amplification of single cells or purified RNA. Use Table 2 (page 15) to select the appropriate protocol based on starting material and downstream analysis. The protocol “Amplificatio ...
The pyruvate dehydrogenase complex of the chemol
... which lacks pyruvate decarboxylase, dihydrolipoyl transacetylase and dihydrolipoamide dehydrogenase activities and is unable to grow on minimal medium lacking acetate whereas PDH positive strains can (Guest et al., 1983). Cosmid 5.1, plasmids pTHI081 and pTHI0251 were transformed into E. coli JRG134 ...
... which lacks pyruvate decarboxylase, dihydrolipoyl transacetylase and dihydrolipoamide dehydrogenase activities and is unable to grow on minimal medium lacking acetate whereas PDH positive strains can (Guest et al., 1983). Cosmid 5.1, plasmids pTHI081 and pTHI0251 were transformed into E. coli JRG134 ...
TARGET: a new method for predicting protein subcellular
... Supplementary data: Accessible as online-only from the publisher. ...
... Supplementary data: Accessible as online-only from the publisher. ...
... The hydrophobic effect is less important for DNA since the bases are polar and largely solvent exposed. (1 1/2 pts) Electrostatic interactions, which have little effect on protein stability, have a large effect on the stability of dsDNA due to charge repulsion between the phosphate groups on each st ...
Chapter 2
... imagine an almost limitless variety of peptides. For example, there are 2050 or slightly more than 1.12 ⫻ 1065 possible sequences for polypeptides with just 50 amino acid residues. ...
... imagine an almost limitless variety of peptides. For example, there are 2050 or slightly more than 1.12 ⫻ 1065 possible sequences for polypeptides with just 50 amino acid residues. ...
Full Text - BioTechniques
... in this study was approximately 230 µm. Following a proper syringe washing procedure, no clogging of syringes or sample cross-contaminations were observed. Furthermore, the syringes can be siliconized to prevent the buildup of air bubbles. With inkjet and pin technologies, bubbles have been reported ...
... in this study was approximately 230 µm. Following a proper syringe washing procedure, no clogging of syringes or sample cross-contaminations were observed. Furthermore, the syringes can be siliconized to prevent the buildup of air bubbles. With inkjet and pin technologies, bubbles have been reported ...
Answers to Scoring in Scrabble (English Word Play)
... the .{3} specifies the intervening codon. To allow some but not all amino acids to occupy this “lysine rich region” we would have to write the regex for each one allowed and separate them with the OR symbol | called “pipe”. (11d) Why might a researcher be interested in looking for secondary structur ...
... the .{3} specifies the intervening codon. To allow some but not all amino acids to occupy this “lysine rich region” we would have to write the regex for each one allowed and separate them with the OR symbol | called “pipe”. (11d) Why might a researcher be interested in looking for secondary structur ...
CHAPTER 3 STRUCTURAL ELEMENTS OF
... 1. helix-loop-helix: it is present in many Ca2+ binding proteins (calmodulin, parvalbumin, troponin C ) or DNA binding proteins 2. β hairpin : two antiparallel β-strands are kept together by a short loop of 2-5 residues. 3. greek key: four β strands (minimum), two short loops and one long loop are n ...
... 1. helix-loop-helix: it is present in many Ca2+ binding proteins (calmodulin, parvalbumin, troponin C ) or DNA binding proteins 2. β hairpin : two antiparallel β-strands are kept together by a short loop of 2-5 residues. 3. greek key: four β strands (minimum), two short loops and one long loop are n ...
Whey Protein: A Functional Food
... Individuals with HIV commonly have low levels of GSH . Several studies have sought to address this by testing the effect of whey protein on the GSH levels of HIV-positive subjects. In one instance, 18 participants were randomised to receive daily doses of 45g whey protein from two different products ...
... Individuals with HIV commonly have low levels of GSH . Several studies have sought to address this by testing the effect of whey protein on the GSH levels of HIV-positive subjects. In one instance, 18 participants were randomised to receive daily doses of 45g whey protein from two different products ...
PDF - Oxford Academic - Oxford University Press
... Aanes et al. summarize the developments in this area and detail important advances in our understanding of transcriptome turnover during the transition from maternal to zygotic genome activity during early development. A timely question in the light of the ENCODE and other genome-wide annotation pro ...
... Aanes et al. summarize the developments in this area and detail important advances in our understanding of transcriptome turnover during the transition from maternal to zygotic genome activity during early development. A timely question in the light of the ENCODE and other genome-wide annotation pro ...
Supplementary information for Ronshaugen, McGinnis
... Cloning arthropod Hox genes. Full length Artemia franciscana Ubx and Antp protein coding sequence was obtained from mRNA extracted from Artemia nauplii collected 5 days after hatching. A fragment of the Artemia Ubx and Antp homeodomain was obtained using primers and cycling conditions similar to Pen ...
... Cloning arthropod Hox genes. Full length Artemia franciscana Ubx and Antp protein coding sequence was obtained from mRNA extracted from Artemia nauplii collected 5 days after hatching. A fragment of the Artemia Ubx and Antp homeodomain was obtained using primers and cycling conditions similar to Pen ...
Final published version
... Manipulation of NADH-dependent steps, and particularly disruption of the las-located lactate dehydrogenase (ldh) gene in Lactococcus lactis, is common to engineering strategies envisaging the accumulation of reduced end products other than lactate. Reverse transcription-PCR experiments revealed that ...
... Manipulation of NADH-dependent steps, and particularly disruption of the las-located lactate dehydrogenase (ldh) gene in Lactococcus lactis, is common to engineering strategies envisaging the accumulation of reduced end products other than lactate. Reverse transcription-PCR experiments revealed that ...
Effect of Six Decades of Selective Breeding on
... To evaluate the extent of the genetic change and its effects on the seed protein composition of soybean cultivars released during the past 60 years, representative ancestral cultivars and those derived from selective breeding were grown in a side-by-side comparison. Total seed protein content, deter ...
... To evaluate the extent of the genetic change and its effects on the seed protein composition of soybean cultivars released during the past 60 years, representative ancestral cultivars and those derived from selective breeding were grown in a side-by-side comparison. Total seed protein content, deter ...
Protein reutilisation in corms of Colchicum autumnale
... different proteolytic systems: vacuolar proteolysis, selective nuclear and cytosolic proteolysis, and organellar proteolysis. Because each cellular compartment is af- ...
... different proteolytic systems: vacuolar proteolysis, selective nuclear and cytosolic proteolysis, and organellar proteolysis. Because each cellular compartment is af- ...
Gene expression
Gene expression is the process by which information from a gene is used in the synthesis of a functional gene product. These products are often proteins, but in non-protein coding genes such as transfer RNA (tRNA) or small nuclear RNA (snRNA) genes, the product is a functional RNA.The process of gene expression is used by all known life - eukaryotes (including multicellular organisms), prokaryotes (bacteria and archaea), and utilized by viruses - to generate the macromolecular machinery for life.Several steps in the gene expression process may be modulated, including the transcription, RNA splicing, translation, and post-translational modification of a protein. Gene regulation gives the cell control over structure and function, and is the basis for cellular differentiation, morphogenesis and the versatility and adaptability of any organism. Gene regulation may also serve as a substrate for evolutionary change, since control of the timing, location, and amount of gene expression can have a profound effect on the functions (actions) of the gene in a cell or in a multicellular organism.In genetics, gene expression is the most fundamental level at which the genotype gives rise to the phenotype, i.e. observable trait. The genetic code stored in DNA is ""interpreted"" by gene expression, and the properties of the expression give rise to the organism's phenotype. Such phenotypes are often expressed by the synthesis of proteins that control the organism's shape, or that act as enzymes catalysing specific metabolic pathways characterising the organism.