UNIT 2. Structure and function of proteins.
... • The conformation of a protein is the one of lowest Gibbs free energy accessible to its sequence within a physiological time frame. Folding is under thermodynamic and kinetic control. • Molten-globule: condensed intermediate on the folding pathway that contains much of the secondary structure eleme ...
... • The conformation of a protein is the one of lowest Gibbs free energy accessible to its sequence within a physiological time frame. Folding is under thermodynamic and kinetic control. • Molten-globule: condensed intermediate on the folding pathway that contains much of the secondary structure eleme ...
2.3. Three-Dimensional structure and function of proteins.
... - Some of them show specific biological roles, but in other cases they are just part of the main structural and functional peptide. ...
... - Some of them show specific biological roles, but in other cases they are just part of the main structural and functional peptide. ...
The phosphorylation of proteins: a major mechanism for biological
... the protein kinases and their control than we d o about the phosphatases. This picture may change, however, because a number of laboratories, most notably that of Philip Cohen in Dundee, are bringing the latter set of enzymes t o the fore. Today, however, I would like t o restrict my discussion to t ...
... the protein kinases and their control than we d o about the phosphatases. This picture may change, however, because a number of laboratories, most notably that of Philip Cohen in Dundee, are bringing the latter set of enzymes t o the fore. Today, however, I would like t o restrict my discussion to t ...
Pavel Doležal
... 2) NADH dehydrogenase complex in hydrogenosomes We purified and cloned two subunits of hydrogenosomal NADH dehydrogenase complex and showed that it can reduce a variety of electron carriers including ubiquinone, but unlike the mitochondrial enzyme it can also reduce ferredoxin. Phylogenetic analyses ...
... 2) NADH dehydrogenase complex in hydrogenosomes We purified and cloned two subunits of hydrogenosomal NADH dehydrogenase complex and showed that it can reduce a variety of electron carriers including ubiquinone, but unlike the mitochondrial enzyme it can also reduce ferredoxin. Phylogenetic analyses ...
Pavel Doležal
... 2) NADH dehydrogenase complex in hydrogenosomes We purified and cloned two subunits of hydrogenosomal NADH dehydrogenase complex and showed that it can reduce a variety of electron carriers including ubiquinone, but unlike the mitochondrial enzyme it can also reduce ferredoxin. Phylogenetic analyses ...
... 2) NADH dehydrogenase complex in hydrogenosomes We purified and cloned two subunits of hydrogenosomal NADH dehydrogenase complex and showed that it can reduce a variety of electron carriers including ubiquinone, but unlike the mitochondrial enzyme it can also reduce ferredoxin. Phylogenetic analyses ...
1. Which of the following are connected by hydrogen bonds? A
... PanI is a gene in cod fish that codes for an integral membrane protein called pantophysin. Two alleles of the gene, PanIA and PanIB, code for versions of pantophysin, that differ by four amino acids in one region of the protein. Samples of cod fish were collected from 23 locations in the north Atlan ...
... PanI is a gene in cod fish that codes for an integral membrane protein called pantophysin. Two alleles of the gene, PanIA and PanIB, code for versions of pantophysin, that differ by four amino acids in one region of the protein. Samples of cod fish were collected from 23 locations in the north Atlan ...
EXPRESSION OF EQUIVALENT CLONOTYPES IN BALB/c
... identified by GC the sequencer products at all positions were hydrolyzed to their free amino acids (22) and subjected to amino acid analysis. This method of identification while having about the same sensitivity as GC permits the observation of the levels of almost all amino acids in a single assay. ...
... identified by GC the sequencer products at all positions were hydrolyzed to their free amino acids (22) and subjected to amino acid analysis. This method of identification while having about the same sensitivity as GC permits the observation of the levels of almost all amino acids in a single assay. ...
Exploring Mouse Protein Function via Multiple Approaches
... Recent advances in sequencing technology have identified a large number of proteins that perform a wide variety of functions in cellular activities. Knowledge of protein function is crucial to understanding the mechanisms behind cellular processes and preventing and treating disease. However, most o ...
... Recent advances in sequencing technology have identified a large number of proteins that perform a wide variety of functions in cellular activities. Knowledge of protein function is crucial to understanding the mechanisms behind cellular processes and preventing and treating disease. However, most o ...
Methods and approaches for the comprehensive characterization
... characterization and quantification of cellular proteomes using mass spectrometry. Physiol Genomics 33: 3–11, 2008. First published December 27, 2007; doi:10.1152/physiolgenomics.00292.2007.—Proteomics has been proposed as one of the key technologies in the postgenomic era. So far, however, the comp ...
... characterization and quantification of cellular proteomes using mass spectrometry. Physiol Genomics 33: 3–11, 2008. First published December 27, 2007; doi:10.1152/physiolgenomics.00292.2007.—Proteomics has been proposed as one of the key technologies in the postgenomic era. So far, however, the comp ...
TFE3 contains two activation domains, one acidic and the other
... suggesting that these regions are important for function. Using two different assays, we show that the Inland C-terminal activation domains of TFE3 act synergistically. This synergism explains in part the ability of TFE3S to act as a dominant negative. Our domain analysis of TFE3 is incorporated int ...
... suggesting that these regions are important for function. Using two different assays, we show that the Inland C-terminal activation domains of TFE3 act synergistically. This synergism explains in part the ability of TFE3S to act as a dominant negative. Our domain analysis of TFE3 is incorporated int ...
Full Text - Journal of Pharmaceutical, Chemical and
... Ginkgo biloba leaves mainly contain these two physiological active ingredients: flavonoids and terpene lactones. Flavonoids have an important role in regulating plant growth and development. Anthocyanidin reductase is a key enzyme involved in the flavonoids biosynthesis. Based on the EST sequence, t ...
... Ginkgo biloba leaves mainly contain these two physiological active ingredients: flavonoids and terpene lactones. Flavonoids have an important role in regulating plant growth and development. Anthocyanidin reductase is a key enzyme involved in the flavonoids biosynthesis. Based on the EST sequence, t ...
Journal of the Science of Food and Agriculture
... substitutes for cereals in gluten-free diets.2 Furthermore, pseudocereals contain relatively high amounts of dietary ®bre, which improves lipid metabolism and takes part in the prevention of LDL-C oxidation.3,4 After processing, these plants can be used as ¯ours or ¯akes or in biscuits and breakfast ...
... substitutes for cereals in gluten-free diets.2 Furthermore, pseudocereals contain relatively high amounts of dietary ®bre, which improves lipid metabolism and takes part in the prevention of LDL-C oxidation.3,4 After processing, these plants can be used as ¯ours or ¯akes or in biscuits and breakfast ...
mschi
... The development of efficient microbial processes for the production of flavonoids has been a metabolic engineering goal for the past several years, primarily due to the purported health-promoting effects of these compounds. Although significant strides have been made recently in improving strain titers ...
... The development of efficient microbial processes for the production of flavonoids has been a metabolic engineering goal for the past several years, primarily due to the purported health-promoting effects of these compounds. Although significant strides have been made recently in improving strain titers ...
heartsprotein.adv.pdf
... All proteins are long chains of amino acids. The unique structure of each different protein is determined by how this protein folds. This situation is similar to that you observed in the exploration activity, where two pieces of origami paper that looked the same could be folded into two unique look ...
... All proteins are long chains of amino acids. The unique structure of each different protein is determined by how this protein folds. This situation is similar to that you observed in the exploration activity, where two pieces of origami paper that looked the same could be folded into two unique look ...
The proteome of Saccharomyces cerevisiae mitochondria
... mitochondria only in the presence of a ⌬ (Fig. 3A): Lap3, an aminopeptidase of the cysteine protease family (38, 39); Nat2, an essential protein reported to be a protein N-acetyl transferase (40); Afg1, a member of the AAA family of ATPases (41); Gif1, a protein required for the normal G1 phase of ...
... mitochondria only in the presence of a ⌬ (Fig. 3A): Lap3, an aminopeptidase of the cysteine protease family (38, 39); Nat2, an essential protein reported to be a protein N-acetyl transferase (40); Afg1, a member of the AAA family of ATPases (41); Gif1, a protein required for the normal G1 phase of ...
Ch 5 Biomolc Strc & Fxn
... • Functional proteins are one or more polypeptides twisted, folded, and coiled into a unique shape • A protein’s amino acid sequence determines its three-dimensional structure • The structure determines its function (form follows ...
... • Functional proteins are one or more polypeptides twisted, folded, and coiled into a unique shape • A protein’s amino acid sequence determines its three-dimensional structure • The structure determines its function (form follows ...
Protein structure
... Many of the images in this powerpoint presentation are from Bioinformatics and Functional Genomics by Jonathan Pevsner (ISBN 0-471-21004-8). Copyright © 2003 by John Wiley & Sons, Inc. These images and materials may not be used without permission from the publisher. We welcome instructors to use the ...
... Many of the images in this powerpoint presentation are from Bioinformatics and Functional Genomics by Jonathan Pevsner (ISBN 0-471-21004-8). Copyright © 2003 by John Wiley & Sons, Inc. These images and materials may not be used without permission from the publisher. We welcome instructors to use the ...
Print - Circulation Research
... some other proteinase inhibitors, such as TLCK or leupeptin, which can penetrate cells more easily than pepstatin, may block nonlysosomal as well as lysosomal proteinases and also may produce nonspecific toxic effects which theoretically might alter proteolysis secondarily, independent of their acti ...
... some other proteinase inhibitors, such as TLCK or leupeptin, which can penetrate cells more easily than pepstatin, may block nonlysosomal as well as lysosomal proteinases and also may produce nonspecific toxic effects which theoretically might alter proteolysis secondarily, independent of their acti ...
The Calcium-Binding Activity of a Vacuole
... Most members of the dehydrin superfamily of related genes are expressed during periods of low water content or during exposure to environmental stresses where osmotic stress is a component of the stress mechanism (Skriver and Mundy, 1990; Close et al., 1993b; Close, 1997). Included in the dehydrin s ...
... Most members of the dehydrin superfamily of related genes are expressed during periods of low water content or during exposure to environmental stresses where osmotic stress is a component of the stress mechanism (Skriver and Mundy, 1990; Close et al., 1993b; Close, 1997). Included in the dehydrin s ...
Identification and characterization of the ergochrome gene cluster in
... and clavorubin could be detected as well as prominent signals of the yellow ergochrome secalonic acid. Figure 7 clearly demonstrates difference in yellow pigment signals at UV 332 nm between the OE TF and the wild type. The marked signals at 34, 35 and 36 min correspond to a m/z value of 637.1554, m ...
... and clavorubin could be detected as well as prominent signals of the yellow ergochrome secalonic acid. Figure 7 clearly demonstrates difference in yellow pigment signals at UV 332 nm between the OE TF and the wild type. The marked signals at 34, 35 and 36 min correspond to a m/z value of 637.1554, m ...
Sequence-Specific Deoxyribonucleic Acid (DNA) Recognition by
... common to all nuclear receptors and binds to 9-bp DNA sequences as a monomer. Here we describe the solution structure of the SF1 DBD in complex with an atypical sequence in the proximal promoter region of the inhibin-! gene that encodes a subunit of a reproductive hormone. SF1 forms a specific ...
... common to all nuclear receptors and binds to 9-bp DNA sequences as a monomer. Here we describe the solution structure of the SF1 DBD in complex with an atypical sequence in the proximal promoter region of the inhibin-! gene that encodes a subunit of a reproductive hormone. SF1 forms a specific ...
Ces locus embedded proteins control the non
... The adjacent to cesAB located gene cesT encodes a putative type II thioesterase (TEII), an enzyme which is often found in association with NRPSs. In contrast to the type I thioesterases, which are an integrated part of the NRPS catalyzing the product release, external TEIIs reactivate the catalytic ...
... The adjacent to cesAB located gene cesT encodes a putative type II thioesterase (TEII), an enzyme which is often found in association with NRPSs. In contrast to the type I thioesterases, which are an integrated part of the NRPS catalyzing the product release, external TEIIs reactivate the catalytic ...
NUCLEOTIDE and PROTEIN databases
... a wide range of web-based retrieval and analysis tools Is built primarily from the submission of sequence data from authors (i.e BankIt) and from bulk submission of high-throughput data from sequencing centre (i.e. Sequin) ...
... a wide range of web-based retrieval and analysis tools Is built primarily from the submission of sequence data from authors (i.e BankIt) and from bulk submission of high-throughput data from sequencing centre (i.e. Sequin) ...
Gene expression
Gene expression is the process by which information from a gene is used in the synthesis of a functional gene product. These products are often proteins, but in non-protein coding genes such as transfer RNA (tRNA) or small nuclear RNA (snRNA) genes, the product is a functional RNA.The process of gene expression is used by all known life - eukaryotes (including multicellular organisms), prokaryotes (bacteria and archaea), and utilized by viruses - to generate the macromolecular machinery for life.Several steps in the gene expression process may be modulated, including the transcription, RNA splicing, translation, and post-translational modification of a protein. Gene regulation gives the cell control over structure and function, and is the basis for cellular differentiation, morphogenesis and the versatility and adaptability of any organism. Gene regulation may also serve as a substrate for evolutionary change, since control of the timing, location, and amount of gene expression can have a profound effect on the functions (actions) of the gene in a cell or in a multicellular organism.In genetics, gene expression is the most fundamental level at which the genotype gives rise to the phenotype, i.e. observable trait. The genetic code stored in DNA is ""interpreted"" by gene expression, and the properties of the expression give rise to the organism's phenotype. Such phenotypes are often expressed by the synthesis of proteins that control the organism's shape, or that act as enzymes catalysing specific metabolic pathways characterising the organism.