Green Fluorescent Protein
... GFP is used within various fields of biology like molecular biology, neuroscience and cell biology. It can be used as a reporter gene, cell marker, fusion tag, and for quantitative monitoring of gene expression. GFP’s fluorescence characteristic can also be used in methods designed for sensing vario ...
... GFP is used within various fields of biology like molecular biology, neuroscience and cell biology. It can be used as a reporter gene, cell marker, fusion tag, and for quantitative monitoring of gene expression. GFP’s fluorescence characteristic can also be used in methods designed for sensing vario ...
Health relevance of intestinal protein fermentation in young
... Knowledge about the undigested protein fractions and their fermentability throughout the different parts of the intestine is still scarce. Usually, protein in animal feed is determined roughly as N × 6.25 and further characterized by the standardized ileal amino acid digestibility, whereas the relat ...
... Knowledge about the undigested protein fractions and their fermentability throughout the different parts of the intestine is still scarce. Usually, protein in animal feed is determined roughly as N × 6.25 and further characterized by the standardized ileal amino acid digestibility, whereas the relat ...
Drug Development
... • Absorption - Passes GI track into blood stream • Distribution - Gets to target tissue (blood brain barrier) • Metabolism – Not readily metabolized • Excretion – Not readily secreted • Toxicity – Not toxic to other cells or tissues ...
... • Absorption - Passes GI track into blood stream • Distribution - Gets to target tissue (blood brain barrier) • Metabolism – Not readily metabolized • Excretion – Not readily secreted • Toxicity – Not toxic to other cells or tissues ...
Nitrogen Balance and Protein Requirements: Definition and
... they must be introduced with the diet in a proportion that will fit with the organism’s metabolic needs. On the other hand, in the absence of dietary NEAA, despite the theoretical capability of the body to synthesise them, nitrogen will be needed for their de novo synthesis. This nitrogen in turn mu ...
... they must be introduced with the diet in a proportion that will fit with the organism’s metabolic needs. On the other hand, in the absence of dietary NEAA, despite the theoretical capability of the body to synthesise them, nitrogen will be needed for their de novo synthesis. This nitrogen in turn mu ...
Cell-Free Phospholipid Biosynthesis by Gene
... and implementation of complex genetic and metabolic pathways that can support essential cellular functions. Here, we show that phospholipid biosynthesis, a multiple-step process involved in cell membrane homeostasis, can be reconstituted starting from the genes encoding for all necessary proteins. A ...
... and implementation of complex genetic and metabolic pathways that can support essential cellular functions. Here, we show that phospholipid biosynthesis, a multiple-step process involved in cell membrane homeostasis, can be reconstituted starting from the genes encoding for all necessary proteins. A ...
Tetrahydrobiopterin and its functions
... Tetrahydrobiopterin (BH4) is essential for diverse processes and is ubiquitously present in all tissues of higher organisms. It is well established as essential cofactor for various enzyme activities to activate dioxygen, and for less defined functions at the cellular level. The latter function of B ...
... Tetrahydrobiopterin (BH4) is essential for diverse processes and is ubiquitously present in all tissues of higher organisms. It is well established as essential cofactor for various enzyme activities to activate dioxygen, and for less defined functions at the cellular level. The latter function of B ...
EPICENTRE Enzyme Catalog
... activity. Klenow DNA polymerase blunt ends doublestranded DNA with singlestranded overhangs. The 3´→5´ exonuclease activity removes 3´ overhangs and the 5´→3´ polymerization activity fills in 5´ overhangs. ...
... activity. Klenow DNA polymerase blunt ends doublestranded DNA with singlestranded overhangs. The 3´→5´ exonuclease activity removes 3´ overhangs and the 5´→3´ polymerization activity fills in 5´ overhangs. ...
Poon, Andy: Predicting Phosphorylation: A critique of the NetPhos program and potential alternatives
... The purpose of using quantitative pattern matching was to possibly identify known motifs common to phosphorylated proteins. Perhaps documented motifs existed, initially attributed to other protein features, which may be indicative of phosphorylation as well. (For instance, perhaps these databases r ...
... The purpose of using quantitative pattern matching was to possibly identify known motifs common to phosphorylated proteins. Perhaps documented motifs existed, initially attributed to other protein features, which may be indicative of phosphorylation as well. (For instance, perhaps these databases r ...
A Rice Plastidial Nucleotide Sugar Epimerase Is Involved in
... Genetic analysis indicated that the phd1 phenotype was controlled by a single recessive gene that did not co-segregate with the T-DNA insertion, and hence map-based cloning was carried out. The PHD1 locus was physically delimited to a 72-kb region on the short arm of chromosome 1. This region contai ...
... Genetic analysis indicated that the phd1 phenotype was controlled by a single recessive gene that did not co-segregate with the T-DNA insertion, and hence map-based cloning was carried out. The PHD1 locus was physically delimited to a 72-kb region on the short arm of chromosome 1. This region contai ...
Production of Polyclonal Antibodies against Sucrose Transporter
... consisted of one cycle at 94 oC for 2 min, 30 cycles at 94 o C for 30 s, 55 oC for 60 s and 72 oC for 2 min, and a final extension step at 72 oC for 7 min. The amplified product was analyzed by 1% agarose gel electrophoresis. Recombinant plasmids were extracted from two clones using the Roche High P ...
... consisted of one cycle at 94 oC for 2 min, 30 cycles at 94 o C for 30 s, 55 oC for 60 s and 72 oC for 2 min, and a final extension step at 72 oC for 7 min. The amplified product was analyzed by 1% agarose gel electrophoresis. Recombinant plasmids were extracted from two clones using the Roche High P ...
See Source - Pentelute Lab
... resolution LCMS (Figure 1b–d). We conducted additional sortagging studies to investigate two aspects of the oligoglycine αthioester: varying the C-terminal amino acid (Xaa) or varying the number of glycine residues. It has been demonstrated that the reactivity of a peptide αthioester is dependent up ...
... resolution LCMS (Figure 1b–d). We conducted additional sortagging studies to investigate two aspects of the oligoglycine αthioester: varying the C-terminal amino acid (Xaa) or varying the number of glycine residues. It has been demonstrated that the reactivity of a peptide αthioester is dependent up ...
Pr-P-105
... leaf discs of cultivar Anhui-l were irradiated by UV-B as above method. Leaves were sampled and frozen in liquid nitrogen and then stored at -70°C until RNA extraction. Expression profiles of TBG and TBP genes were analyzed by reverse transcriptase polymerase chain reaction method (II. ...
... leaf discs of cultivar Anhui-l were irradiated by UV-B as above method. Leaves were sampled and frozen in liquid nitrogen and then stored at -70°C until RNA extraction. Expression profiles of TBG and TBP genes were analyzed by reverse transcriptase polymerase chain reaction method (II. ...
Nucleotide sequence of a segment of Drosophila mitochondrial DNA
... Figure 1 . A map of the 0. yakuba mtDNA molecule showing the r e l a t i v e l o c a t i o n s of the A+T-ricF region ( c r o s s h a t c h e d ) , the two rRNA genes ( d o t t e d ) , the o r i g i n ( 0 ) , and d i r e c t i o n (R) of r e p l i c a t i o n , EcoRI and Hindi 11 s i t e s and fragm ...
... Figure 1 . A map of the 0. yakuba mtDNA molecule showing the r e l a t i v e l o c a t i o n s of the A+T-ricF region ( c r o s s h a t c h e d ) , the two rRNA genes ( d o t t e d ) , the o r i g i n ( 0 ) , and d i r e c t i o n (R) of r e p l i c a t i o n , EcoRI and Hindi 11 s i t e s and fragm ...
endoplasmic reticulum stress and lipid metabolism
... sequence (UASIno), which is recognized by a heterodimeric transcription factor of Ino2p and Ino4p. Opi1p represses the Ino2p/Ino4p pathway by binding directly to Ino2p and recruiting a transcriptio ...
... sequence (UASIno), which is recognized by a heterodimeric transcription factor of Ino2p and Ino4p. Opi1p represses the Ino2p/Ino4p pathway by binding directly to Ino2p and recruiting a transcriptio ...
Three multidomain esterases from the cellulolytic
... Three enzymes carrying esterase domains have been identified in the rumen cellulolytic anaerobe Ruminococcus flavefaciens 17. The newly characterized CesA gene product (768 amino acids) includes an N-terminal acetylesterase domain and an unidentified C-terminal domain, while the previously character ...
... Three enzymes carrying esterase domains have been identified in the rumen cellulolytic anaerobe Ruminococcus flavefaciens 17. The newly characterized CesA gene product (768 amino acids) includes an N-terminal acetylesterase domain and an unidentified C-terminal domain, while the previously character ...
Plasma membrane - HCC Learning Web
... • 3-1 List the functions of the plasma membrane and the structural features that enable it to perform those functions. • 3-2 Describe the organelles of a typical cell, and indicate the specific functions of each. • 3-3 Explain the functions of the cell nucleus and discuss the nature and importance o ...
... • 3-1 List the functions of the plasma membrane and the structural features that enable it to perform those functions. • 3-2 Describe the organelles of a typical cell, and indicate the specific functions of each. • 3-3 Explain the functions of the cell nucleus and discuss the nature and importance o ...
Branched-Chain Amino Acids in Exercise
... Downloaded from jn.nutrition.org by guest on October 10, 2014 ...
... Downloaded from jn.nutrition.org by guest on October 10, 2014 ...
"Genetic Methods of Polymer Synthesis". In: Encyclopedia of
... into the appropriate expression host. Alternatively, short, repetitive amino acid sequences that are found in naturally occurring proteins can be linked together to form an artificial protein (one that does not exist in nature). Finally, since the folding of certain amino acid motifs is understood, a ...
... into the appropriate expression host. Alternatively, short, repetitive amino acid sequences that are found in naturally occurring proteins can be linked together to form an artificial protein (one that does not exist in nature). Finally, since the folding of certain amino acid motifs is understood, a ...
Identification of prokaryotic homologues indicates an endosymbiotic
... the generation of reactive oxygen species, which could have been an early function of alternative oxidases (Gomes et al., 2001). AOX is a low molecular weight mitochondrial protein (approximately 34 kDa) that is encoded in the nucleus. The enzyme is translated as a precursor protein with a mitochond ...
... the generation of reactive oxygen species, which could have been an early function of alternative oxidases (Gomes et al., 2001). AOX is a low molecular weight mitochondrial protein (approximately 34 kDa) that is encoded in the nucleus. The enzyme is translated as a precursor protein with a mitochond ...
A Major Surface Protein on Group A Streptococci Is a
... as important constituents of human saliva (17, 18). The surfaces of both Gram-negative and Gram-positive bacteria have been shown to contain molecules that bind to fibronectin and lysozyme (19-21). While a fibronectin binding protein in Staphylococcus aureus has been identified (22), characterized ( ...
... as important constituents of human saliva (17, 18). The surfaces of both Gram-negative and Gram-positive bacteria have been shown to contain molecules that bind to fibronectin and lysozyme (19-21). While a fibronectin binding protein in Staphylococcus aureus has been identified (22), characterized ( ...
Conservation and relative importance of residues across protein
... consequently have different evolutionary pressure for their conservation. The degree of conservation of each interface residue can be defined in terms of sequence entropy at that position in the polypeptide chain across all of the homologous proteins. At least three types of contacts can be identifi ...
... consequently have different evolutionary pressure for their conservation. The degree of conservation of each interface residue can be defined in terms of sequence entropy at that position in the polypeptide chain across all of the homologous proteins. At least three types of contacts can be identifi ...
food produced from glyphosate tolerant sugar beet line 77
... into sugar beet plants by the addition of two new genes. One of these genes encodes the CP4-EPSPS protein, a key enzyme in the biosynthesis of aromatic amino acids in plants and microbes that is not sensitive to applications of glyphosate. The second gene, the gox gene, encodes the glyphosate oxidor ...
... into sugar beet plants by the addition of two new genes. One of these genes encodes the CP4-EPSPS protein, a key enzyme in the biosynthesis of aromatic amino acids in plants and microbes that is not sensitive to applications of glyphosate. The second gene, the gox gene, encodes the glyphosate oxidor ...
Discovery of genes in the Pacific oyster (Crassostrea gigas) involved
... 50 mL non-sterile of seawater. Sixteen oysters held in 8 L of seawater were inoculated via a 3 hour immersion bath with V. vulnificus at an initial concentration of 4.56x1019 CFU/L. Control oysters (n=16) were likewise placed in 8L of seawater. Following exposure, oysters were harvested aseptically ...
... 50 mL non-sterile of seawater. Sixteen oysters held in 8 L of seawater were inoculated via a 3 hour immersion bath with V. vulnificus at an initial concentration of 4.56x1019 CFU/L. Control oysters (n=16) were likewise placed in 8L of seawater. Following exposure, oysters were harvested aseptically ...
food produced from glyphosate
... into sugar beet plants by the addition of two new genes. One of these genes encodes the CP4-EPSPS protein, a key enzyme in the biosynthesis of aromatic amino acids in plants and microbes that is not sensitive to applications of glyphosate. The second gene, the gox gene, encodes the glyphosate oxidor ...
... into sugar beet plants by the addition of two new genes. One of these genes encodes the CP4-EPSPS protein, a key enzyme in the biosynthesis of aromatic amino acids in plants and microbes that is not sensitive to applications of glyphosate. The second gene, the gox gene, encodes the glyphosate oxidor ...
From Sequence to Function
... Sequence and structural comparison can usually give only limited information, however, and comprehensively characterizing the function of an uncharacterized protein in a cell or organism will always require additional experimental investigations on the purified protein in vitro as well as cell biolo ...
... Sequence and structural comparison can usually give only limited information, however, and comprehensively characterizing the function of an uncharacterized protein in a cell or organism will always require additional experimental investigations on the purified protein in vitro as well as cell biolo ...
Gene expression
Gene expression is the process by which information from a gene is used in the synthesis of a functional gene product. These products are often proteins, but in non-protein coding genes such as transfer RNA (tRNA) or small nuclear RNA (snRNA) genes, the product is a functional RNA.The process of gene expression is used by all known life - eukaryotes (including multicellular organisms), prokaryotes (bacteria and archaea), and utilized by viruses - to generate the macromolecular machinery for life.Several steps in the gene expression process may be modulated, including the transcription, RNA splicing, translation, and post-translational modification of a protein. Gene regulation gives the cell control over structure and function, and is the basis for cellular differentiation, morphogenesis and the versatility and adaptability of any organism. Gene regulation may also serve as a substrate for evolutionary change, since control of the timing, location, and amount of gene expression can have a profound effect on the functions (actions) of the gene in a cell or in a multicellular organism.In genetics, gene expression is the most fundamental level at which the genotype gives rise to the phenotype, i.e. observable trait. The genetic code stored in DNA is ""interpreted"" by gene expression, and the properties of the expression give rise to the organism's phenotype. Such phenotypes are often expressed by the synthesis of proteins that control the organism's shape, or that act as enzymes catalysing specific metabolic pathways characterising the organism.