Lehninger Principles of Biochemistry
... of a functional biological product, whether protein or RNA, is referred to as a gene. A cell typically has many thousands of genes, and DNA molecules, not surprisingly, tend to be very large. The storage and transmission of biological information are the only known functions of DNA. RNAs have a broa ...
... of a functional biological product, whether protein or RNA, is referred to as a gene. A cell typically has many thousands of genes, and DNA molecules, not surprisingly, tend to be very large. The storage and transmission of biological information are the only known functions of DNA. RNAs have a broa ...
Protein sequence analysis
... PAM (Percent Accepted Mutations): on the basis of comparisons among many pairs of very similar protein sequences (at least 85% identical, ie., homologous sequences), Margaret Dayhoff constructed a mutation probability matrix comparing many pairs of protein sequences to determine the empirical freque ...
... PAM (Percent Accepted Mutations): on the basis of comparisons among many pairs of very similar protein sequences (at least 85% identical, ie., homologous sequences), Margaret Dayhoff constructed a mutation probability matrix comparing many pairs of protein sequences to determine the empirical freque ...
The trimethoprim-resistant dihydrofolate reductase associated with
... with the R plasmid R388 was isolated from strains that overproduce the enzyme. It was purified to apparent homogeneity by affinity chromatography and two consecutive gel filtration steps under native and denaturing conditions. The purified enzyme is composed of four identical subunits with molecular ...
... with the R plasmid R388 was isolated from strains that overproduce the enzyme. It was purified to apparent homogeneity by affinity chromatography and two consecutive gel filtration steps under native and denaturing conditions. The purified enzyme is composed of four identical subunits with molecular ...
DO ANTIBODIES RECOGNIZE AMINO ACID SIDE CHAINS OF
... amino acids present in CDR2 and 2 amino acids present in FR2 of 61112 and 61B8 (positions 48-61) . The role of the internal image residues 56 and 57 in contributing to a1-like expression was assessed with the SMA1 peptide, identical to MAI except for conservative substitutions at the two relevant po ...
... amino acids present in CDR2 and 2 amino acids present in FR2 of 61112 and 61B8 (positions 48-61) . The role of the internal image residues 56 and 57 in contributing to a1-like expression was assessed with the SMA1 peptide, identical to MAI except for conservative substitutions at the two relevant po ...
IJEB 48(4) 373-377
... revealed the presence of Ala, Asp, Glu, Gly, Ser and Thr. The earlier studies on azo-dye bound peptide revealed that it contained Pro, Leu/Ile, Val and Gly as major components and Glu, Phe, Ser, Ala and Asp as minor components19. Terayama and Takeuchi20 isolated a fraction on hydrolysis of azo-dye b ...
... revealed the presence of Ala, Asp, Glu, Gly, Ser and Thr. The earlier studies on azo-dye bound peptide revealed that it contained Pro, Leu/Ile, Val and Gly as major components and Glu, Phe, Ser, Ala and Asp as minor components19. Terayama and Takeuchi20 isolated a fraction on hydrolysis of azo-dye b ...
e Study of RNA Polymerase Pausing by Optical Traps
... biology of living organisms. It is responsible for DNA transcription, the first step of genetic expression, and is therefore highly regulated by the cell. If transcription is not regulated, cellular processes can become unsynchronized, possibly leading to uncontrolled cell growth and cancer. Transcr ...
... biology of living organisms. It is responsible for DNA transcription, the first step of genetic expression, and is therefore highly regulated by the cell. If transcription is not regulated, cellular processes can become unsynchronized, possibly leading to uncontrolled cell growth and cancer. Transcr ...
國立清華大學 - 罕見疾病基金會
... metabolism in eukaryotic cells. This organelle is formed by two phospholipid membranes, outer- and inner-membrane, to separate three compartments which from outside to inside are called cytosol, inter-membrane space and matrix. There are many protein complexes on the inner-membrane which participate ...
... metabolism in eukaryotic cells. This organelle is formed by two phospholipid membranes, outer- and inner-membrane, to separate three compartments which from outside to inside are called cytosol, inter-membrane space and matrix. There are many protein complexes on the inner-membrane which participate ...
How to obtain labeled proteins and what to do with... Marlon J Hinner and Kai Johnsson
... rarely rigorously checked, in some cases small tags have been reported to perform significantly better than autofluorescent protein fusions. As may be expected, this involves proteins that are part of a tightly packed structure or involved in a translocation process. For example, an impact of tag si ...
... rarely rigorously checked, in some cases small tags have been reported to perform significantly better than autofluorescent protein fusions. As may be expected, this involves proteins that are part of a tightly packed structure or involved in a translocation process. For example, an impact of tag si ...
Chylomicron Remnants and Nonesterified Fatty Involved in Lipogenesis in Rats
... Within the liver sinusoids, the nonesterified fatty acids dissociate from albumin and enter hepatocytes by a mechanism thought to involve specific fatty acid transporters (14,15). There is little or no information comparing the regulation of metabolic processes by chylomicron remnants compared with ...
... Within the liver sinusoids, the nonesterified fatty acids dissociate from albumin and enter hepatocytes by a mechanism thought to involve specific fatty acid transporters (14,15). There is little or no information comparing the regulation of metabolic processes by chylomicron remnants compared with ...
the Gene Ontology
... Use GO for……. Determining which classes of gene products are over-represented or under-represented. Grouping gene products by biological function. Relating a protein’s location to its function. Focusing on particular biological pathways and functions (hypothesis-driven data interrogation). ...
... Use GO for……. Determining which classes of gene products are over-represented or under-represented. Grouping gene products by biological function. Relating a protein’s location to its function. Focusing on particular biological pathways and functions (hypothesis-driven data interrogation). ...
Structural and Thermal Stability Characterization of Protein
... Table.2.3.Showing the solubility of C18SA modified hemoglobin in different organic solvents.200ul of 50uM C18SA modified BSA was mixed with 1mL of each organic solvent to test the solubility ...
... Table.2.3.Showing the solubility of C18SA modified hemoglobin in different organic solvents.200ul of 50uM C18SA modified BSA was mixed with 1mL of each organic solvent to test the solubility ...
Thermal unfolding of proteins at high pH range studied by UV
... protein unfolding. Buried tyrosine residues are not affected by the pH. The unfolding of the protein molecule leads to the exposure of these residues at the protein surface and therefore tyrosyl ionization is detected. In this paper we measured the degree of ionization of tyrosine residues and used ...
... protein unfolding. Buried tyrosine residues are not affected by the pH. The unfolding of the protein molecule leads to the exposure of these residues at the protein surface and therefore tyrosyl ionization is detected. In this paper we measured the degree of ionization of tyrosine residues and used ...
Lipid profiling and transcriptomic analysis reveals a functional
... pharmacological doses of E2 in humans inhibits GH-regulated endocrine (e.g., IGF-I) and metabolic (e.g., lipid oxidation, protein synthesis) effects [22,23] but these effects are attenuated when E2 is administered transdermally, suggesting that liver is the major target of regulatory cross-talk betw ...
... pharmacological doses of E2 in humans inhibits GH-regulated endocrine (e.g., IGF-I) and metabolic (e.g., lipid oxidation, protein synthesis) effects [22,23] but these effects are attenuated when E2 is administered transdermally, suggesting that liver is the major target of regulatory cross-talk betw ...
Polyamines
... Polyamines may also play a role in the regulation of DNA replication and cell division, and are implicated in the control of senescence and morphogenesis (Evans and Malmberg, 1989; Galston and Sawnhey, 1990). ...
... Polyamines may also play a role in the regulation of DNA replication and cell division, and are implicated in the control of senescence and morphogenesis (Evans and Malmberg, 1989; Galston and Sawnhey, 1990). ...
FEBS Letters
... As a ¢rst step towards the identi¢cation of the gene encoding Dxr, the characteristic enzyme for the MEP pathway, a suitable gene probe was developed. The deduced dxr sequence of E. coli (P45568) was therefore compared to the hypothetical Dxr-like protein sequence of the sequenced genome of Synechoc ...
... As a ¢rst step towards the identi¢cation of the gene encoding Dxr, the characteristic enzyme for the MEP pathway, a suitable gene probe was developed. The deduced dxr sequence of E. coli (P45568) was therefore compared to the hypothetical Dxr-like protein sequence of the sequenced genome of Synechoc ...
Tissue-specific codon usage and the expression of
... (36 genes), and vulva (42 genes). By analyzing expression patterns from only two studies, we limited ourselves to fewer data than are available in large compilations of many expression studies. On the other hand, the expression data we have used are comparable (both studies used the GeneChip HuGeneF ...
... (36 genes), and vulva (42 genes). By analyzing expression patterns from only two studies, we limited ourselves to fewer data than are available in large compilations of many expression studies. On the other hand, the expression data we have used are comparable (both studies used the GeneChip HuGeneF ...
Probability-Based Scoring Function as a Software
... Probability-based scoring function. INTRODUCTION The identification of differentially expressed proteins by employing experimental techniques and tools is a goal of proteomics. After protein isolation by various techniques, including two dimensional- gel electrophoresis (2D-PAGE), a protein of inter ...
... Probability-based scoring function. INTRODUCTION The identification of differentially expressed proteins by employing experimental techniques and tools is a goal of proteomics. After protein isolation by various techniques, including two dimensional- gel electrophoresis (2D-PAGE), a protein of inter ...
Supplementary Materials
... overexpression samples showed a value of 0.72. These significant correlations for both cases suggest that the underlying genetic contributions are the same across different ethanol concentrations. Modular analysis of fitness profiles The pathway-level analysis of the fitness profiles was performed ...
... overexpression samples showed a value of 0.72. These significant correlations for both cases suggest that the underlying genetic contributions are the same across different ethanol concentrations. Modular analysis of fitness profiles The pathway-level analysis of the fitness profiles was performed ...
ISH ISH ISH ISH ISH
... In addition, perfusion results in ISH data with low background due to clearance of blood cells from the tissue Fixation by immersion, on the other hand, should be used when perfusion is not possible - for example with clinical samples or embryonic tissues ...
... In addition, perfusion results in ISH data with low background due to clearance of blood cells from the tissue Fixation by immersion, on the other hand, should be used when perfusion is not possible - for example with clinical samples or embryonic tissues ...
Histoplasma capsulatum an effective mechanism for trans-cell wall transfer
... including the H and M antigens, pluripotent glycoproteins that elicit both humoral and T cell-mediated immune responses (Zancope-Oliveira et al., 1999; Fisher and Woods, 2000; Deepe and Gibbons, 2001a), and a virulence-related, phase-specific protein (YPS3p), which is found at the cell wall (Bohse a ...
... including the H and M antigens, pluripotent glycoproteins that elicit both humoral and T cell-mediated immune responses (Zancope-Oliveira et al., 1999; Fisher and Woods, 2000; Deepe and Gibbons, 2001a), and a virulence-related, phase-specific protein (YPS3p), which is found at the cell wall (Bohse a ...
Genetics Test 3 Review Presentation
... • The specialized heterochromatic chromosomal region at which sister chromatids remain attached after replication, and the site to which spindle fibers attach to the chromosome during cell division. Location of the centromere determines the shape of the chromosome during the anaphase portion of cell ...
... • The specialized heterochromatic chromosomal region at which sister chromatids remain attached after replication, and the site to which spindle fibers attach to the chromosome during cell division. Location of the centromere determines the shape of the chromosome during the anaphase portion of cell ...
The Strategic Use of Ruminally Protected Amino Acids in Dairy
... nutrition of the dairy cow; (3) point out where we need more knowledge and how this lack of knowledge could contribute to inconsistent results; and (4) describe how we go about balancing for AA. When AA Balance Is Critical In our opinion, there are times when AA balance is critical. They are: 1. Wh ...
... nutrition of the dairy cow; (3) point out where we need more knowledge and how this lack of knowledge could contribute to inconsistent results; and (4) describe how we go about balancing for AA. When AA Balance Is Critical In our opinion, there are times when AA balance is critical. They are: 1. Wh ...
GEFs: master regulators of G
... GEF for Rab5, are effectors of Ras. Such mechanisms allow a signal to diverge into multiple pathways10. Signaling networks also involve indirect connections. For example, Rho-family GEFs Vav and Tiam contain PH domains directly C-terminal to their GEF domains. These phosphoinositolbinding domains mi ...
... GEF for Rab5, are effectors of Ras. Such mechanisms allow a signal to diverge into multiple pathways10. Signaling networks also involve indirect connections. For example, Rho-family GEFs Vav and Tiam contain PH domains directly C-terminal to their GEF domains. These phosphoinositolbinding domains mi ...
Alu Background Information
... functional rt gene. Interestingly, in addition to reverse transcribing RNA to DNA, the L1 rt also produces single-stranded nicks in DNA. In the current model, the rt enzyme produces a nick at a chromosomal locus containing the sequence AATTTT. The polyadenylated "tail" of the Alu transcript (-AAAA) ...
... functional rt gene. Interestingly, in addition to reverse transcribing RNA to DNA, the L1 rt also produces single-stranded nicks in DNA. In the current model, the rt enzyme produces a nick at a chromosomal locus containing the sequence AATTTT. The polyadenylated "tail" of the Alu transcript (-AAAA) ...
Gene expression
Gene expression is the process by which information from a gene is used in the synthesis of a functional gene product. These products are often proteins, but in non-protein coding genes such as transfer RNA (tRNA) or small nuclear RNA (snRNA) genes, the product is a functional RNA.The process of gene expression is used by all known life - eukaryotes (including multicellular organisms), prokaryotes (bacteria and archaea), and utilized by viruses - to generate the macromolecular machinery for life.Several steps in the gene expression process may be modulated, including the transcription, RNA splicing, translation, and post-translational modification of a protein. Gene regulation gives the cell control over structure and function, and is the basis for cellular differentiation, morphogenesis and the versatility and adaptability of any organism. Gene regulation may also serve as a substrate for evolutionary change, since control of the timing, location, and amount of gene expression can have a profound effect on the functions (actions) of the gene in a cell or in a multicellular organism.In genetics, gene expression is the most fundamental level at which the genotype gives rise to the phenotype, i.e. observable trait. The genetic code stored in DNA is ""interpreted"" by gene expression, and the properties of the expression give rise to the organism's phenotype. Such phenotypes are often expressed by the synthesis of proteins that control the organism's shape, or that act as enzymes catalysing specific metabolic pathways characterising the organism.