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Lecture Protein Metabolism
... Sources of Amino Acids for Host Animal 1. Microbial proteins Quantity determined by: a) Fermentability of the feed b) Quantity of feed consumed c) Nitrogen available to microorganisms 2. Undegraded feed proteins (UIP) Quantity will vary in relation to: a) Degradability of feed proteins b) Quantity ...
... Sources of Amino Acids for Host Animal 1. Microbial proteins Quantity determined by: a) Fermentability of the feed b) Quantity of feed consumed c) Nitrogen available to microorganisms 2. Undegraded feed proteins (UIP) Quantity will vary in relation to: a) Degradability of feed proteins b) Quantity ...
Dr. John Perozich's Full CV
... project by Anne McMahon in which 103 protein sequences of sialidases from living organisms and influenza virus neuraminidase were aligned to elucidate structural, functional and phylogenetic relationships in the enzyme family. Comparative Analysis of the Adenylate-Forming Superfamily. Poster. Dani L ...
... project by Anne McMahon in which 103 protein sequences of sialidases from living organisms and influenza virus neuraminidase were aligned to elucidate structural, functional and phylogenetic relationships in the enzyme family. Comparative Analysis of the Adenylate-Forming Superfamily. Poster. Dani L ...
Applied and Environmental Microbiologyy
... For flow-chamber experiments, the strains were tagged with the green fluorescent protein (GFP). This was accomplished by the insertion of a miniTn7PA1/04/03-gfp-T0T1 transposon cassette into the chromosomes of target strains using the suicide construct pBK-miniTn7-gfp3 (25). Plasmid pBK-miniTn7-gfp3 ...
... For flow-chamber experiments, the strains were tagged with the green fluorescent protein (GFP). This was accomplished by the insertion of a miniTn7PA1/04/03-gfp-T0T1 transposon cassette into the chromosomes of target strains using the suicide construct pBK-miniTn7-gfp3 (25). Plasmid pBK-miniTn7-gfp3 ...
Red/ET Recombination
... E. coli using homologous recombination mediated by phage protein pairs, either RecE/RecT or Reda/Redb. Recombineering with Red/ET differs from other DNA engineering technologies, such as cutting and pasting with restriction enzymes, PCR, DNA ligase, because it is ...
... E. coli using homologous recombination mediated by phage protein pairs, either RecE/RecT or Reda/Redb. Recombineering with Red/ET differs from other DNA engineering technologies, such as cutting and pasting with restriction enzymes, PCR, DNA ligase, because it is ...
Characterization of Acetyl-CoA Carboxylases in the Basal
... domains. Biotin carboxylase (BC) is responsible for carboxylating the biotin cofactor, which is then transferred to the carboxyl transferase (CT) domain via the biotin carrier protein (BCCP) which serves as an attachment point for the biotin. The CT domain then carboxylates the acetyl CoA, convertin ...
... domains. Biotin carboxylase (BC) is responsible for carboxylating the biotin cofactor, which is then transferred to the carboxyl transferase (CT) domain via the biotin carrier protein (BCCP) which serves as an attachment point for the biotin. The CT domain then carboxylates the acetyl CoA, convertin ...
Enzyme Mechanisms - Illinois Institute of Technology
... cloned inserts in expression vectors, viz. make RNA or even protein from it RNA: stick an efficient promoter next to the cloning site; vector DNA transcribed in vitro using SP6 RNA polymerase This can be used as a way of making radiolabeled RNA ...
... cloned inserts in expression vectors, viz. make RNA or even protein from it RNA: stick an efficient promoter next to the cloning site; vector DNA transcribed in vitro using SP6 RNA polymerase This can be used as a way of making radiolabeled RNA ...
... A1,2 is the conjugate of C1,2 & U1,2 is the conjugate of G1,2:(A2 x C2 & G2 x U2) With 6 h-bonds at positions 1 and 2 between codon and anticodon, third base is inconsequential With 4 h-bonds at positions 1 and 2 third base is essential With 5 h-bonds middle pyrimidine renders third base inconsequen ...
Developmental Regulation of Genes Encoding Universal Stress
... endodermal epithelium, a highly potent chemical barrier for protection against intruding microbes. Sequences associated with ESTs, SAGE Tags and microarray probes are functional genomic tools appropriate for dissecting gene functions.22 These tools can provide expression data to facilitate forward a ...
... endodermal epithelium, a highly potent chemical barrier for protection against intruding microbes. Sequences associated with ESTs, SAGE Tags and microarray probes are functional genomic tools appropriate for dissecting gene functions.22 These tools can provide expression data to facilitate forward a ...
... f) promoter: Binds RNA polymerase, begins mRNA production. g) antibiotic resistance gene: Bacteria with the plasmid will be resistant to the antibiotic, allowing selection for those bacterial that contain the plasmid. h) origin of replication. Allows plasmid to be replicated in bacteria. iv) Provide ...
AN ABSTRACT OF THE THESIS OF Sara R. Fassio for the degree of
... modulated by a hierarchy of transcriptional regulators (17). These regulators include the transcription factors AphA and AphB, which positively regulate transcription of the twocomponent regulatory system tcpPH. The membrane-bound protein complex TcpPH works with the membrane-bound protein complex T ...
... modulated by a hierarchy of transcriptional regulators (17). These regulators include the transcription factors AphA and AphB, which positively regulate transcription of the twocomponent regulatory system tcpPH. The membrane-bound protein complex TcpPH works with the membrane-bound protein complex T ...
Structural adaptation of enzymes to low
... were used in turn as keywords to check further for the presence of other proteins from the same source in the databanks. A literature scrutiny was undertaken to ensure that only proteins with proved ‘cold-adapted’ features and clear enzymatic activity were considered among those previously retrieved ...
... were used in turn as keywords to check further for the presence of other proteins from the same source in the databanks. A literature scrutiny was undertaken to ensure that only proteins with proved ‘cold-adapted’ features and clear enzymatic activity were considered among those previously retrieved ...
Spatial restriction of AChR gene expression to
... AChR and mRNAs encoding AChR subunits, persist at synaptic sites after denervation (Fambrough, 1979; Fontaine and Changeux, 1989; Goldman and Staple, 1989; Witzemann et al., 1991). In order to determine whether synapse-specific transcription of the delta subunit gene was maintained after denervation ...
... AChR and mRNAs encoding AChR subunits, persist at synaptic sites after denervation (Fambrough, 1979; Fontaine and Changeux, 1989; Goldman and Staple, 1989; Witzemann et al., 1991). In order to determine whether synapse-specific transcription of the delta subunit gene was maintained after denervation ...
Isolation and Characterization of Rice MADS Box Gene
... from dicotyledons such as Arabidopsis,2'3 snapdragon,4'5 and petunia,6 and from monocotyledons such as rice, 7 " 10 maize,11^13 and sorghum.14 The conserved domain in MADS from many species has been a good target for examining the evolutionary diversification of eukaryotic morphology.15 However, the ...
... from dicotyledons such as Arabidopsis,2'3 snapdragon,4'5 and petunia,6 and from monocotyledons such as rice, 7 " 10 maize,11^13 and sorghum.14 The conserved domain in MADS from many species has been a good target for examining the evolutionary diversification of eukaryotic morphology.15 However, the ...
The Old World monkey DAZ (Deleted in
... infertile men (3) led to the identification of three different AZF loci (AZFa, -b and -c). Microdeletions of AZFa–c could be detected in 5–15% of the infertile men, suggesting that the deleted regions of the Y chromosome contain genes essential for spermatogenesis (4,5). One candidate gene in the AZ ...
... infertile men (3) led to the identification of three different AZF loci (AZFa, -b and -c). Microdeletions of AZFa–c could be detected in 5–15% of the infertile men, suggesting that the deleted regions of the Y chromosome contain genes essential for spermatogenesis (4,5). One candidate gene in the AZ ...
(De)regulation of key enzyme steps in the shikimate pathway and
... present in A. methanolica. DAHP synthase 1 (DS1) is a 160 kDa enzyme associated non-covalently with a dimeric CM protein, thus forming a heteromeric two-enzyme complex. The two enzyme activities can be separated by Q-Sepharose anion-exchange chromatography, yielding a dimeric CM protein with a fivef ...
... present in A. methanolica. DAHP synthase 1 (DS1) is a 160 kDa enzyme associated non-covalently with a dimeric CM protein, thus forming a heteromeric two-enzyme complex. The two enzyme activities can be separated by Q-Sepharose anion-exchange chromatography, yielding a dimeric CM protein with a fivef ...
Monocyte chemoattrac- tant protein
... Fig. 1. Laser capture microdissection (LCM) of glomeruli in kidney sections. Tissue sections of the same specimen are shown before (A) and after (B) LCM. Arrows indicate glomeruli. (C) Quantities of cytokine mRNA expressions in glomeruli and (D) interstitium of kidney sections from PBS-treated, empt ...
... Fig. 1. Laser capture microdissection (LCM) of glomeruli in kidney sections. Tissue sections of the same specimen are shown before (A) and after (B) LCM. Arrows indicate glomeruli. (C) Quantities of cytokine mRNA expressions in glomeruli and (D) interstitium of kidney sections from PBS-treated, empt ...
Atomic contacts in protein structures. A detailed analysis of atomic
... Billions of years of evolution optimized proteins to fulfill their functions efficiently. Regardless whether the protein functions as enzyme, molecular motor, transport protein, or receptor, a prerequisite for optimal function is a fine-tuned structural and dynamical framework, either directly or in ...
... Billions of years of evolution optimized proteins to fulfill their functions efficiently. Regardless whether the protein functions as enzyme, molecular motor, transport protein, or receptor, a prerequisite for optimal function is a fine-tuned structural and dynamical framework, either directly or in ...
The bHLH Class Protein pMesogenin1 Can Specify Paraxial
... Many of the most potent inducers of mesoderm in this system are signaling molecules that act upstream, in developmental terms, of transcriptional regulators that specify the expression of mesoderm genes (Smith, 1995). Among these signals, Activin, a member of the TGF family, induces panmesodermal t ...
... Many of the most potent inducers of mesoderm in this system are signaling molecules that act upstream, in developmental terms, of transcriptional regulators that specify the expression of mesoderm genes (Smith, 1995). Among these signals, Activin, a member of the TGF family, induces panmesodermal t ...
Identification and Analysis of Dicer Associated Proteins in
... Dicer is a multidomain RNase III like enzyme involved in the initial steps of RNA interference (RNAi) and microRNA (miRNA) pathways, two mechanisms of posttranscriptional gene silencing conserved throughout most eukaryotic species. Dicer has been shown to be necessary and sufficient to cut long dsRN ...
... Dicer is a multidomain RNase III like enzyme involved in the initial steps of RNA interference (RNAi) and microRNA (miRNA) pathways, two mechanisms of posttranscriptional gene silencing conserved throughout most eukaryotic species. Dicer has been shown to be necessary and sufficient to cut long dsRN ...
Over-expression of a putative poplar glycosyltransferase gene
... Family 1 glycosyltransferases catalyse the glycosylation of small molecules and play an important role in maintaining cell homeostasis and regulating plant growth and development. In this study, a putative glycosyltransferase gene of family 1, PtGT1, was cloned from poplar (Populus tomentosa Carr.). ...
... Family 1 glycosyltransferases catalyse the glycosylation of small molecules and play an important role in maintaining cell homeostasis and regulating plant growth and development. In this study, a putative glycosyltransferase gene of family 1, PtGT1, was cloned from poplar (Populus tomentosa Carr.). ...
Expression of an Entire Bacterial Operon in Plants
... which are transcribed to polycistronic pre-mRNAs and on a structural basis, can be defined as "operon-like" (summarized in Blumenthal, 2004). However, unlike in prokaryotes, the polycistronic pre-mRNAs are processed to monocistronic (sometimes dicistronic) mature mRNAs and are co-regulated by virtu ...
... which are transcribed to polycistronic pre-mRNAs and on a structural basis, can be defined as "operon-like" (summarized in Blumenthal, 2004). However, unlike in prokaryotes, the polycistronic pre-mRNAs are processed to monocistronic (sometimes dicistronic) mature mRNAs and are co-regulated by virtu ...
Molecular and Structural Characterization of
... et al., 2000, 2001; Zouhar et al., 2001) and are thought to be essential for the recognition of the aglycone moiety. Furthermore, Ala-467 was demonstrated to make contact with the methoxy group of DIMBOAGlc. While Trp-378 and Phe-198 are conserved in rye at the corresponding positions, Phe-205, Phe- ...
... et al., 2000, 2001; Zouhar et al., 2001) and are thought to be essential for the recognition of the aglycone moiety. Furthermore, Ala-467 was demonstrated to make contact with the methoxy group of DIMBOAGlc. While Trp-378 and Phe-198 are conserved in rye at the corresponding positions, Phe-205, Phe- ...
Processing of 20S prerRNA to 18S ribosomal RNA in yeast requires
... characterization of the trans-acting factors involved in this process constituted one of the `key questions' in the early nineties (Woolford and Warner, 1991). During the past decade, thanks to the development of new biochemical, genetic and in silico technologies, dozens of these components have be ...
... characterization of the trans-acting factors involved in this process constituted one of the `key questions' in the early nineties (Woolford and Warner, 1991). During the past decade, thanks to the development of new biochemical, genetic and in silico technologies, dozens of these components have be ...
- Digital Commons @ Otterbein
... Selection is driven by challenges inherent to an environment. The heritable genotypes of different individuals of the species change over time through random, heritable changes (alteration of the sequence of nucleotides; mutations) in an organism’s germ line DNA, or germ line RNA in some cases (e.g. ...
... Selection is driven by challenges inherent to an environment. The heritable genotypes of different individuals of the species change over time through random, heritable changes (alteration of the sequence of nucleotides; mutations) in an organism’s germ line DNA, or germ line RNA in some cases (e.g. ...
Journal of Bacteriology
... leguminosarum bv. viciae strain 248 with two similar genes from Brucella abortus is discussed. ground of other biovars (27). The outer membrane proteins of strain 248 can be divided into four prominent antigen groups, designated I through IV. Two of these, antigen groups II and III, are severely dec ...
... leguminosarum bv. viciae strain 248 with two similar genes from Brucella abortus is discussed. ground of other biovars (27). The outer membrane proteins of strain 248 can be divided into four prominent antigen groups, designated I through IV. Two of these, antigen groups II and III, are severely dec ...
Gene expression
Gene expression is the process by which information from a gene is used in the synthesis of a functional gene product. These products are often proteins, but in non-protein coding genes such as transfer RNA (tRNA) or small nuclear RNA (snRNA) genes, the product is a functional RNA.The process of gene expression is used by all known life - eukaryotes (including multicellular organisms), prokaryotes (bacteria and archaea), and utilized by viruses - to generate the macromolecular machinery for life.Several steps in the gene expression process may be modulated, including the transcription, RNA splicing, translation, and post-translational modification of a protein. Gene regulation gives the cell control over structure and function, and is the basis for cellular differentiation, morphogenesis and the versatility and adaptability of any organism. Gene regulation may also serve as a substrate for evolutionary change, since control of the timing, location, and amount of gene expression can have a profound effect on the functions (actions) of the gene in a cell or in a multicellular organism.In genetics, gene expression is the most fundamental level at which the genotype gives rise to the phenotype, i.e. observable trait. The genetic code stored in DNA is ""interpreted"" by gene expression, and the properties of the expression give rise to the organism's phenotype. Such phenotypes are often expressed by the synthesis of proteins that control the organism's shape, or that act as enzymes catalysing specific metabolic pathways characterising the organism.