Increased Functional Half-life of Fibroblast Growth Factor
... true considering that protein unfolding, which is dependent on protein stability, is often a necessary first step for the irreversible denaturation pathway resulting from exposure of the reactive free cysteine. In this paper we report the functional half-life of the Ala66 → Cys mutant form of FGF-1. ...
... true considering that protein unfolding, which is dependent on protein stability, is often a necessary first step for the irreversible denaturation pathway resulting from exposure of the reactive free cysteine. In this paper we report the functional half-life of the Ala66 → Cys mutant form of FGF-1. ...
Isolation of Vibrio harveyi Acyl Carrier Protein and the fabG, acpP
... protein 78% identical to E. coli 3-ketoacyl-ACP reductase (21); acpP (nucleotides 1282 to 1512; see below); and fabF (nucleotides 1609 to 2853), encoding a 43.1-kDa protein 76% identical to E. coli 3-ketoacyl-ACP synthase II. The latter protein was originally reported as 3-ketoacyl-ACP synthase IV, ...
... protein 78% identical to E. coli 3-ketoacyl-ACP reductase (21); acpP (nucleotides 1282 to 1512; see below); and fabF (nucleotides 1609 to 2853), encoding a 43.1-kDa protein 76% identical to E. coli 3-ketoacyl-ACP synthase II. The latter protein was originally reported as 3-ketoacyl-ACP synthase IV, ...
Document
... Yeast as a genetically tractable organism to identify drug targets, establish cellular disease models, diagnosis, and for screening of receptor ligands and lead compounds ...
... Yeast as a genetically tractable organism to identify drug targets, establish cellular disease models, diagnosis, and for screening of receptor ligands and lead compounds ...
Chapter 5
... • Most proteins probably go through several states on their way to a stable structure Normal protein ...
... • Most proteins probably go through several states on their way to a stable structure Normal protein ...
Document
... process, so it is advantageous to an organism to complement dietary intake. In mammals, cholesterol production is regulated by intracellular cholesterol concentration and by the hormones glucagon and insulin. Regulation is mediated by an elegant system of transcriptional regulation of the gene e ...
... process, so it is advantageous to an organism to complement dietary intake. In mammals, cholesterol production is regulated by intracellular cholesterol concentration and by the hormones glucagon and insulin. Regulation is mediated by an elegant system of transcriptional regulation of the gene e ...
HiTrap Chelating HP 1 ml and 5 ml
... and peptides on the basis of their affinity for metal ions that have been immobilized by chelation. Certain amino acids (e.g. histidine and cysteine) form complexes with the chelated metals around neutral pH (pH 6–8). It is primarily the histidine content of a protein that is responsible for its bin ...
... and peptides on the basis of their affinity for metal ions that have been immobilized by chelation. Certain amino acids (e.g. histidine and cysteine) form complexes with the chelated metals around neutral pH (pH 6–8). It is primarily the histidine content of a protein that is responsible for its bin ...
Chapter 3 powerpoint File - District 196 e
... • Secretory vesicles • Contain products to be discharged from the cell • Fuse with plasma membrane and release contents into extracellular environment ...
... • Secretory vesicles • Contain products to be discharged from the cell • Fuse with plasma membrane and release contents into extracellular environment ...
Mutating your DNA - Montgomery`s Hedrick Life Science
... If one or more mutations caused the second codon to be either TAA, TAG or UGA, describe the protein resulting from this nonsense substitution: ...
... If one or more mutations caused the second codon to be either TAA, TAG or UGA, describe the protein resulting from this nonsense substitution: ...
A-level Human Biology Question Paper Unit 04 - Bodies and
... Wrapping a person in blankets leads to an immediate warming of the skin. This stimulates thermoreceptors in the skin. This stimulation of thermoreceptors in the skin causes the fall in core body temperature seen in the first ten minutes. Suggest how. ...
... Wrapping a person in blankets leads to an immediate warming of the skin. This stimulates thermoreceptors in the skin. This stimulation of thermoreceptors in the skin causes the fall in core body temperature seen in the first ten minutes. Suggest how. ...
J Molecular Biology 307:939-949, 2001
... sequence-to-sequence searches.1 ± 6 This is the result of identifying what residues are preferred in the conserved sequence regions of the family members and of not using sequence regions that are not conserved. Searching multiple alignment databases with multiple alignment queries is superior in ma ...
... sequence-to-sequence searches.1 ± 6 This is the result of identifying what residues are preferred in the conserved sequence regions of the family members and of not using sequence regions that are not conserved. Searching multiple alignment databases with multiple alignment queries is superior in ma ...
Comparison of environmental and isolate Sulfobacillus genomes
... regions were extracted and trimmed with Sickle (available https://github.com/najoshi/sickle), allowing only paired-end reads with length >60 and quality scores >20. For the reference database, 186 sequences were downloaded from the SILVA SSU database representing the 174 sequences of ‘Family XVII In ...
... regions were extracted and trimmed with Sickle (available https://github.com/najoshi/sickle), allowing only paired-end reads with length >60 and quality scores >20. For the reference database, 186 sequences were downloaded from the SILVA SSU database representing the 174 sequences of ‘Family XVII In ...
Compare insertion and deletion mutations
... If one or more mutations caused the second codon to be either TAA, TAG or UGA, describe the protein resulting from this nonsense substitution: ...
... If one or more mutations caused the second codon to be either TAA, TAG or UGA, describe the protein resulting from this nonsense substitution: ...
Plasma membrane
... • Secretory vesicles • Contain products to be discharged from the cell • Fuse with plasma membrane and release contents into extracellular environment ...
... • Secretory vesicles • Contain products to be discharged from the cell • Fuse with plasma membrane and release contents into extracellular environment ...
DNA sequencing revealed a definitive
... Figure 4. Similarity matrix for the amino acid sequences of the homeo domains of iab-7 and its deuterostome cognates. Numbers represent % aa positional identity, which is 100 x the number of matched aa/60 aa. 72% aa positional identity, respectively (26,28,30,31,32). Also, the HB4 homeo domain share ...
... Figure 4. Similarity matrix for the amino acid sequences of the homeo domains of iab-7 and its deuterostome cognates. Numbers represent % aa positional identity, which is 100 x the number of matched aa/60 aa. 72% aa positional identity, respectively (26,28,30,31,32). Also, the HB4 homeo domain share ...
Gene Expression
... recognized by more than one probe in the ProbeLibrary and the ProbeFinder assay design software can usually suggest more than one primer-probe combination for each target. If one primer-probe combination fails to give satisfactory real-time PCR assay results, the user can easily select one of the ot ...
... recognized by more than one probe in the ProbeLibrary and the ProbeFinder assay design software can usually suggest more than one primer-probe combination for each target. If one primer-probe combination fails to give satisfactory real-time PCR assay results, the user can easily select one of the ot ...
Avian Infectious Bronchitis Virus (IBV)
... This product is developed, designed and sold for research purposes only. It is not intended for human diagnostic or drug purposes or to be administered to humans unless clearly expressed for that purpose by the Food and Drug Administration in the USA or the appropriate regulatory authorities in the ...
... This product is developed, designed and sold for research purposes only. It is not intended for human diagnostic or drug purposes or to be administered to humans unless clearly expressed for that purpose by the Food and Drug Administration in the USA or the appropriate regulatory authorities in the ...
19-6-SA-V1-S1__mcq_a..
... loosely attached to the enzyme and can be separated by dialysis and is essential for enzyme action. 79. _________activity, It is defined as the amount of substrate the enzyme converts (reactions catalyzed), per mg protein in the enzyme preparation, per unit of time 86. This class of enzyme will carr ...
... loosely attached to the enzyme and can be separated by dialysis and is essential for enzyme action. 79. _________activity, It is defined as the amount of substrate the enzyme converts (reactions catalyzed), per mg protein in the enzyme preparation, per unit of time 86. This class of enzyme will carr ...
Database searching
... A Profile HMM is a probabilistic representation of a multiple alignment. A given multiple alignment (of a protein family) is used to build a profile HMM. This model then may be used to find and score less obvious potential matches of new protein sequences. ...
... A Profile HMM is a probabilistic representation of a multiple alignment. A given multiple alignment (of a protein family) is used to build a profile HMM. This model then may be used to find and score less obvious potential matches of new protein sequences. ...
Induction of CDPK (ZmCPK11)- and MAPK
... other signaling molecules such as oligosaccharins and volatile organic compounds (VOCs) emitted by plants (Schilmiller and Howe 2005, Koornneef and Pieterse 2008). JA, its precursors (e.g., 12-oxophytodienoic acid OPDA) and derivatives (e.g., methyl jasmonate MeJA) collectively referred to as jasmon ...
... other signaling molecules such as oligosaccharins and volatile organic compounds (VOCs) emitted by plants (Schilmiller and Howe 2005, Koornneef and Pieterse 2008). JA, its precursors (e.g., 12-oxophytodienoic acid OPDA) and derivatives (e.g., methyl jasmonate MeJA) collectively referred to as jasmon ...
Novel Multiprotein Complexes Identified in the Hyperthermophilic
... identify groups of proteins that work together as complexes in distinct cellular processes. Genome-wide functional analyses suggest that there are 200 –300 core biological functions that are essential to life (1). More often than not the functional units are assemblies composed of multiple proteins ...
... identify groups of proteins that work together as complexes in distinct cellular processes. Genome-wide functional analyses suggest that there are 200 –300 core biological functions that are essential to life (1). More often than not the functional units are assemblies composed of multiple proteins ...
EVIDENCE FOR TWO DISTINCT CLASSES OF STREPTOCOCCAL
... Group A streptococci are human pathogens that infect primarily at the skin or nasopharyngeal mucosa . A major virulence factor present on the streptococcal surface is M protein, a molecule of which there exists more than 80 distinct serological types. M protein provides the streptococcus with the ab ...
... Group A streptococci are human pathogens that infect primarily at the skin or nasopharyngeal mucosa . A major virulence factor present on the streptococcal surface is M protein, a molecule of which there exists more than 80 distinct serological types. M protein provides the streptococcus with the ab ...
The Mouse Prolactin Gene Family Locus
... RT-PCR restriction enzyme analysis of PL-I-related genes. PL-I-related cDNAs were amplified from blastocyst outgrowths or placentas from d 10 of gestation. Amplified products were digested with restriction enzymes capable of differentially cutting PL-I␣, PL-I, and/or PL-I␥. BsaJ1 digests each of th ...
... RT-PCR restriction enzyme analysis of PL-I-related genes. PL-I-related cDNAs were amplified from blastocyst outgrowths or placentas from d 10 of gestation. Amplified products were digested with restriction enzymes capable of differentially cutting PL-I␣, PL-I, and/or PL-I␥. BsaJ1 digests each of th ...
chordin (H-300): sc-28964 - Santa Cruz Biotechnology
... recommended: 1) Western Blotting: use goat anti-rabbit IgG-HRP: sc-2004 (dilution range: 1:2000-1:100,000) or Cruz Marker™ compatible goat antirabbit IgG-HRP: sc-2030 (dilution range: 1:2000-1:5000), Cruz Marker™ Molecular Weight Standards: sc-2035, TBS Blotto A Blocking Reagent: sc-2333 and Western ...
... recommended: 1) Western Blotting: use goat anti-rabbit IgG-HRP: sc-2004 (dilution range: 1:2000-1:100,000) or Cruz Marker™ compatible goat antirabbit IgG-HRP: sc-2030 (dilution range: 1:2000-1:5000), Cruz Marker™ Molecular Weight Standards: sc-2035, TBS Blotto A Blocking Reagent: sc-2333 and Western ...
Załącznik nr 3 do Zarządzenia Rektora PUM…………………….. z
... knows functions of nucleotides, structures of DNA and RNA (primary and secondary) and chromatin structure knows functions of genome, transcriptome and proteome as well as basic methods applied to investigate these; describes processes of DNA replication, repair and recombination, processes of transc ...
... knows functions of nucleotides, structures of DNA and RNA (primary and secondary) and chromatin structure knows functions of genome, transcriptome and proteome as well as basic methods applied to investigate these; describes processes of DNA replication, repair and recombination, processes of transc ...
Amyotrophic lateral sclerosis: Protein
... same peptide derived from the proteolytic digestions of many different, yet homologous proteins. Therefore, when changes in protein expression are quantified on the basis of a small number of peptides, the rate of false positives is likely to be higher than that obtained from the analysis based on a ...
... same peptide derived from the proteolytic digestions of many different, yet homologous proteins. Therefore, when changes in protein expression are quantified on the basis of a small number of peptides, the rate of false positives is likely to be higher than that obtained from the analysis based on a ...
Gene expression
Gene expression is the process by which information from a gene is used in the synthesis of a functional gene product. These products are often proteins, but in non-protein coding genes such as transfer RNA (tRNA) or small nuclear RNA (snRNA) genes, the product is a functional RNA.The process of gene expression is used by all known life - eukaryotes (including multicellular organisms), prokaryotes (bacteria and archaea), and utilized by viruses - to generate the macromolecular machinery for life.Several steps in the gene expression process may be modulated, including the transcription, RNA splicing, translation, and post-translational modification of a protein. Gene regulation gives the cell control over structure and function, and is the basis for cellular differentiation, morphogenesis and the versatility and adaptability of any organism. Gene regulation may also serve as a substrate for evolutionary change, since control of the timing, location, and amount of gene expression can have a profound effect on the functions (actions) of the gene in a cell or in a multicellular organism.In genetics, gene expression is the most fundamental level at which the genotype gives rise to the phenotype, i.e. observable trait. The genetic code stored in DNA is ""interpreted"" by gene expression, and the properties of the expression give rise to the organism's phenotype. Such phenotypes are often expressed by the synthesis of proteins that control the organism's shape, or that act as enzymes catalysing specific metabolic pathways characterising the organism.