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Ch - TeacherWeb
Ch - TeacherWeb

... parental strands of DNA separate, serve as templates and produce DNA molecules that have one strand of parental DNA and one strand of new DNA. 1. unwinding: the double helix is unwound and unzipped by the enzyme DNA helicase a. hydrogen bonds between base pairs are broken b. single-stranded binding ...
NITROGEN BASES in DNA
NITROGEN BASES in DNA

... fishing line (0.5 millimeters) it might stretch as far as 21.2 km (or 13.6 miles) in length which would all have to be packed into a nucleus, the equivalent size of 25 cm in diameter. That is some packaging! ...
Recombinant DNA and Cloning The Impact of Biotechnology
Recombinant DNA and Cloning The Impact of Biotechnology

... Terms to Know • Restriction enzymes: allow the DNA to be cut and spliced at VERY specific locations. • Vectors: carriers of DNA molecules; usually bacteria. • Plasmid: circular DNA found in bacteria. • Recombinant DNA: original carrier DNA + introduced sections of DNA. • Clones: when the bacteria d ...
DNA and Genes
DNA and Genes

... (AT/CG) that are floating around in the cytoplasm pair up forming 2 new strands of DNA ...
Biology Chapter 4
Biology Chapter 4

... ...
DNA Replication Paper Clip Activity
DNA Replication Paper Clip Activity

... You now have a model of the hGH gene (the first ten bases only.) Compare the two chains with each other side-by-side to verify that C bonds with G, and A bonds with T. When this gene replicates in the nucleus of a cell, the double-strand begins to separate at one end. As it separates, new nucleotide ...
Biology Chapter 11-1
Biology Chapter 11-1

... Ex. German Sheppard’s, toy poodles, and Great Danes Hybridization- A cross between dissimilar individuals. (usually between different, but related, species.) Ex. Mules and pigs Mutagens- substances in the environment, such as radiation and chemicals, that cause mutations. Genetic engineering- a form ...
DNA Replication Paper Clip Activity
DNA Replication Paper Clip Activity

... You now have a model of the hGH gene (the first ten bases only.) Compare the two chains with each other side-by-side to verify that C bonds with G, and A bonds with T. When this gene replicates in the nucleus of a cell, the double-strand begins to separate at one end. As it separates, new nucleotide ...
Unit #3 Retake Ticket Unit 3 Retake Ticket
Unit #3 Retake Ticket Unit 3 Retake Ticket

... 1. A change in a single gene is called a _________________ mutation. a. When a nucleotide is deleted a _____________________ mutation occurs. b. When a nucleotide is added, a ______________________ mutation occurs. c. When one nucleotide is changed for another, it is called a ____________________ mu ...
Slide 1
Slide 1

... the DNA molecule at a specific point • Probe – a portion of DNA with a known sequence of bases that is used to find its complimentary strand. ...
Name: DNA Stations Once Mendel`s work was rediscovered in the
Name: DNA Stations Once Mendel`s work was rediscovered in the

... Station 3--Hershey and Chase Watch the video and answer the questions as you go. You may need to watch it more than once. A little background info: Bacteriophages are viruses that infect bacteria. Although they are not living, they do contain DNA. At the time no one knew whether the genetic material ...
DNA Deoxyribonucleic Acid
DNA Deoxyribonucleic Acid

DNA Technology - Dr. Annette M. Parrott
DNA Technology - Dr. Annette M. Parrott

... • DNA polymerase1:exonuclease activity, 2: DNA repair, 3:primary replication enzyme) • Ligasejoins nucleic acid pieces • Primase joins RNA primer to DNA strand • Helicaseunwinds the DNA • Nuclease cuts DNA • Endonuclease cuts DNA internally vs at the ends • Telomerasecatalyzes lengthening of ...
Gel Electrophoresis DNA Fingerprinting
Gel Electrophoresis DNA Fingerprinting

... Where did they come from? • Bacteria! • Natural defense against viral infections • Cut up DNA at various bases sequences • May leave a “stickey end” • May leave “blunt end” ...
Structure of Nucleic Acids
Structure of Nucleic Acids

... The base pairs form a flat plain in the helix, the adenine forming two hydrogen bonds with thymine, and the cytidine forming three bonds with guanine. Using the concept of base pairing, all the enzymes and substrates necessary, the two DNA strands when copied separately, wherever there is adenine in ...
DNA - The Double Helix
DNA - The Double Helix

... all the activities of the cell including cell reproduction, and heredity. How does it do this? The nucleus controls these activities by the chromosomes. Chromosomes are microscopic, threadlike strands composed of the chemical DNA (short for deoxyribonucleic acid). In simple terms, DNA controls the p ...
Ch. 13: DNA, RNA and Proteins
Ch. 13: DNA, RNA and Proteins

... Right side makes a new left half Left side makes a new right half 2 new, identical, complete copies ...
Unit Three “Cell Proliferation and Genetics”
Unit Three “Cell Proliferation and Genetics”

DNA
DNA

... • also used to release energy, but not as efficient as aerobic respiration (less ATP is produced than aerobic) • Two Types: Alcoholic Fermentation and Lactic Acid Fermentation ...
Document
Document

...  Recombine DNA fragments from various sources ...
Ch. 13: DNA, RNA and Proteins
Ch. 13: DNA, RNA and Proteins

... Right side makes a new left half Left side makes a new right half 2 new, identical, complete copies ...
III.C.7 PREPARATION OF THE 32P
III.C.7 PREPARATION OF THE 32P

... • 1µl of g32P –ATP contains approximately 20 pmol of 32P when the isotope is fresh (more III.C.7 ...
Chapter 13-15 Essential Knowledge
Chapter 13-15 Essential Knowledge

... Gene regulation results in differential gene expression, leading to cell specialization. 1. Describe how the following experiments contributed to our knowledge of DNA as the genetic material: a. Watson & Crick, Wilson, Franklin b. Avery-MacLeod-McCarty Experiment c. Hershey-Chase Experiment 2. Why i ...
DNA Review Sheet Answers
DNA Review Sheet Answers

... Before mRNA leaves the nucleus, segments called Introns are cut out in a process called RNA editing.. After this process only the exons are left, which make up mRNA that is ready to travel into the cytoplasm. ...
Unit 5 Molecular Genetics CLASS NOTES
Unit 5 Molecular Genetics CLASS NOTES

... 4) RESULT: Semiconservative replication  Each new DNA contains one OLD strand and one NEW strand ...
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Replisome



The replisome is a complex molecular machine that carries out replication of DNA. The replisome first unwinds double stranded DNA into two single strands. For each of the resulting single strands, a new complementary sequence of DNA is synthesized. The net result is formation of two new double stranded DNA sequences that are exact copies of the original double stranded DNA sequence.In terms of structure, the replisome is composed of two replicative polymerase complexes, one of which synthesizes the leading strand, while the other synthesizes the lagging strand. The replisome is composed of a number of proteins including helicase, RFC, PCNA, gyrase/topoisomerase, SSB/RPA, primase, DNA polymerase I, RNAse H, and ligase.
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