Section 8 – The human genome project
... b. sequencing technology must be accurate and fast; c. computer hardware and software must be available to analyse the sequence data. 3. The technique used for sequencing is called dideoxy chaintermination method. 4. This method relies on making a copy of the DNA template to be sequenced using:a. a ...
... b. sequencing technology must be accurate and fast; c. computer hardware and software must be available to analyse the sequence data. 3. The technique used for sequencing is called dideoxy chaintermination method. 4. This method relies on making a copy of the DNA template to be sequenced using:a. a ...
Lab 1: Split Pea DNA Extraction Questions to consider Where is
... tube down the side so that it forms a layer on top of the pea mixture. Pour until you have about the same amount of alcohol in the tube as pea mixture. 9. Look for clumps of white stringy stuff where the water and alcohol layers meet. 10. Draw the DNA out with the skewer, being careful to be gentle. ...
... tube down the side so that it forms a layer on top of the pea mixture. Pour until you have about the same amount of alcohol in the tube as pea mixture. 9. Look for clumps of white stringy stuff where the water and alcohol layers meet. 10. Draw the DNA out with the skewer, being careful to be gentle. ...
Document
... Nucleotides- A, G, C, T Primer/Loading dye mix (on ice!) Primers to target Portion of the TAS2R38 gene Loading dye for gel Template DNA- cheek DNA from Part I ...
... Nucleotides- A, G, C, T Primer/Loading dye mix (on ice!) Primers to target Portion of the TAS2R38 gene Loading dye for gel Template DNA- cheek DNA from Part I ...
Protein Synthesis
... adenine, cytosine, guanine, and uracil (U). Look at the SIMULATION pane. Is the shown molecule DNA or RNA? How do you know?____________________________________________________________ 2. RNA polymerase is a type of enzyme. Enzymes help chemical reactions occur quickly. Click the Release enzyme butto ...
... adenine, cytosine, guanine, and uracil (U). Look at the SIMULATION pane. Is the shown molecule DNA or RNA? How do you know?____________________________________________________________ 2. RNA polymerase is a type of enzyme. Enzymes help chemical reactions occur quickly. Click the Release enzyme butto ...
DNA – RNA – PROTEIN SYNTHESIS -NOTES-
... Watson and Crick’s model of DNA was called a ____________________________________, in which two strands were wound around each other, like a twisted ladder or spiral ...
... Watson and Crick’s model of DNA was called a ____________________________________, in which two strands were wound around each other, like a twisted ladder or spiral ...
Protein Synthesis - Shenandoah Baptist Church
... 3. Each three letter codon will attract a matching three letter anti-codon of transfer RNA (tRNA) 4. Each tRNA is carrying an amino acid “on its back” 5. As the tRNA molecules line up, their amino acids also line up 6. As the amino acids are lined up, they chemically bond to form a protein ...
... 3. Each three letter codon will attract a matching three letter anti-codon of transfer RNA (tRNA) 4. Each tRNA is carrying an amino acid “on its back” 5. As the tRNA molecules line up, their amino acids also line up 6. As the amino acids are lined up, they chemically bond to form a protein ...
Nucleus - Control Center of cell
... • Chromatin is a substance that contains DNA and proteins. • each strand of chromatin is one molecule of DNA in the nucleus. • During cell growth DNA uncoils and creates proteins. • Chromatin coils into Chromosomes (x shaped structure) when cells ready to divide ...
... • Chromatin is a substance that contains DNA and proteins. • each strand of chromatin is one molecule of DNA in the nucleus. • During cell growth DNA uncoils and creates proteins. • Chromatin coils into Chromosomes (x shaped structure) when cells ready to divide ...
Supplemental Material
... amplified from genomic DNA (FGSC A4 strain) with Taq Platinum DNA polymerase High Fidelity (Invitrogen) using primers ATM AscI and ATM2000 PacI and cloned in the AscI -PacI sites of the pMCB17apx vector (EFIMOV 2003) resulting in the plasmid pMCB17apx-ATM2000. This plasmid was used to transform the ...
... amplified from genomic DNA (FGSC A4 strain) with Taq Platinum DNA polymerase High Fidelity (Invitrogen) using primers ATM AscI and ATM2000 PacI and cloned in the AscI -PacI sites of the pMCB17apx vector (EFIMOV 2003) resulting in the plasmid pMCB17apx-ATM2000. This plasmid was used to transform the ...
Homologous Recombination DNA break repair by homologous
... Transposase multimers make a blunt double-stranded cut at the edge of the inverted repeat termini. Transposase also has a second binding site for DNA that is not sequence-specific, which it uses to bind an insertion target site and make a staggered double-stranded cut. Transposase bound to the trans ...
... Transposase multimers make a blunt double-stranded cut at the edge of the inverted repeat termini. Transposase also has a second binding site for DNA that is not sequence-specific, which it uses to bind an insertion target site and make a staggered double-stranded cut. Transposase bound to the trans ...
Biodosimetry - Arkansas State University
... Damage on/to single strands • Incorrect base due to – Spontaneous reactions – Base analogs – Copying errors ...
... Damage on/to single strands • Incorrect base due to – Spontaneous reactions – Base analogs – Copying errors ...
DNA Review
... structure is often described Helix as a double _______ or a twisted Ladder _______ ...
... structure is often described Helix as a double _______ or a twisted Ladder _______ ...
DNA, RNA and Protein Synthesis Study Guide Applied Bio Name
... c. the number of phosphates d. all of the above V. Short Answer: 1. Briefly outline the steps of replication: DNA helicase unwinds & unzips double helix Single-stranded binding protein holds open the 2 sides of DNA ladder DNA polymerase matches together complementary bases on each side of DNA ...
... c. the number of phosphates d. all of the above V. Short Answer: 1. Briefly outline the steps of replication: DNA helicase unwinds & unzips double helix Single-stranded binding protein holds open the 2 sides of DNA ladder DNA polymerase matches together complementary bases on each side of DNA ...
Transcription & Translation
... the building of a complimentary copy of mRNA. The RNA polymerase enzyme moves along the DNA template and as it moves (RNA) nucleotides are brought into place one by one to form a RNA chain 3. The single stranded RNA molecule called pre-messenger RNA (pre-mRNA) is fully complimentary to the original ...
... the building of a complimentary copy of mRNA. The RNA polymerase enzyme moves along the DNA template and as it moves (RNA) nucleotides are brought into place one by one to form a RNA chain 3. The single stranded RNA molecule called pre-messenger RNA (pre-mRNA) is fully complimentary to the original ...
Replisome
The replisome is a complex molecular machine that carries out replication of DNA. The replisome first unwinds double stranded DNA into two single strands. For each of the resulting single strands, a new complementary sequence of DNA is synthesized. The net result is formation of two new double stranded DNA sequences that are exact copies of the original double stranded DNA sequence.In terms of structure, the replisome is composed of two replicative polymerase complexes, one of which synthesizes the leading strand, while the other synthesizes the lagging strand. The replisome is composed of a number of proteins including helicase, RFC, PCNA, gyrase/topoisomerase, SSB/RPA, primase, DNA polymerase I, RNAse H, and ligase.