Identification of enzymes involved in anaerobic benzene
... the culture was transferred from benzene to a different substrate, the community structure of benzene-, phenoland benzoate-grown cultures was assessed by T-RFLP analysis when approximately 25 mM ferrous iron was produced (Fig. S1). With all substrates, the analysis showed the same dominance of the P ...
... the culture was transferred from benzene to a different substrate, the community structure of benzene-, phenoland benzoate-grown cultures was assessed by T-RFLP analysis when approximately 25 mM ferrous iron was produced (Fig. S1). With all substrates, the analysis showed the same dominance of the P ...
Regulation of transcript encoding the 43K
... Materials and methods). The sequence of the cDNA was determined and both the nucleic acid and deduced amino acid sequences were compared to that for the Torpedo 43K protein (Figs 1 and 2). Xen 43.1 cDNA is 1403 bp long, contains 205 bp of the 5' untranslated region and 1198 bp of protein-coding regi ...
... Materials and methods). The sequence of the cDNA was determined and both the nucleic acid and deduced amino acid sequences were compared to that for the Torpedo 43K protein (Figs 1 and 2). Xen 43.1 cDNA is 1403 bp long, contains 205 bp of the 5' untranslated region and 1198 bp of protein-coding regi ...
Lecture 03, NEW - terpenes + polyketides
... - provides a huge number of potential 3D structures - high degree of biological activity (3) Pathways can be elucidated using labeled precursors, such as mevalonate with a 13C at position 2 - carbon NMR experiments reveal where the label ends up in the completed molecule ...
... - provides a huge number of potential 3D structures - high degree of biological activity (3) Pathways can be elucidated using labeled precursors, such as mevalonate with a 13C at position 2 - carbon NMR experiments reveal where the label ends up in the completed molecule ...
Altered Fermentative Metabolism in
... (PFL1), pyruvate-ferredoxin-oxidoreductase (PFR1, often referred to as PFOR), and the pyruvate dehydrogenase (PDH) complex. As PDH generates NADH, it is presumed that PFL1 and PFR1 are the favored anoxic pathways, with PFL1 representing the dominant pathway in Chlamydomonas. During anoxia, the acety ...
... (PFL1), pyruvate-ferredoxin-oxidoreductase (PFR1, often referred to as PFOR), and the pyruvate dehydrogenase (PDH) complex. As PDH generates NADH, it is presumed that PFL1 and PFR1 are the favored anoxic pathways, with PFL1 representing the dominant pathway in Chlamydomonas. During anoxia, the acety ...
View/Open - VUW research archive - Victoria University of Wellington
... Conditions for extraction and two-dimensional electrophoresis of proteins were established. One hundred and seventy nine proteins were identified by MALDI mass spectrometry of tryptic digests of protein spots excised from Coomassie stained gels. All of the enzymes for conversion of glucose to ethano ...
... Conditions for extraction and two-dimensional electrophoresis of proteins were established. One hundred and seventy nine proteins were identified by MALDI mass spectrometry of tryptic digests of protein spots excised from Coomassie stained gels. All of the enzymes for conversion of glucose to ethano ...
Document
... different components of the body[2]. Of course, the contribution of these components to whole body protein content differs with carcass protein being the largest contributor to whole body protein. Also, the contribution of the different parts to whole body protein changes over time with an increasin ...
... different components of the body[2]. Of course, the contribution of these components to whole body protein content differs with carcass protein being the largest contributor to whole body protein. Also, the contribution of the different parts to whole body protein changes over time with an increasin ...
proteins: three-dimensional structure
... Proteins have historically been classified as either fibrous or globular, depending on their overall morphology. This dichotomy predates methods for determining protein structure on an atomic scale and does not do justice to proteins that contain both stiff, elongated, fibrous regions as well as mor ...
... Proteins have historically been classified as either fibrous or globular, depending on their overall morphology. This dichotomy predates methods for determining protein structure on an atomic scale and does not do justice to proteins that contain both stiff, elongated, fibrous regions as well as mor ...
03-1 Metabolism of carbohydrate
... Pathway Has a Rich History 1910s to 30s, Embden and Meyerhof (Germany), glycolysis in muscle and its extracts: in vitro reconstruction from glycogen to lactic acid; many reactions of lactic acid (muscle) and alcohol (yeast) fermentations are the same; lactic acid is reconverted to carbohydrate in ...
... Pathway Has a Rich History 1910s to 30s, Embden and Meyerhof (Germany), glycolysis in muscle and its extracts: in vitro reconstruction from glycogen to lactic acid; many reactions of lactic acid (muscle) and alcohol (yeast) fermentations are the same; lactic acid is reconverted to carbohydrate in ...
ASPECTS OF ANAEROBIC METABOLISM IN
... participation of glutamate is rather small, and there seems to be no stoichiometric relation to the accumulation of the organic acids. As isotachophoretic analysis did not reveal accumulations of metabolites other than acetate, succinate and propionate, we may conclude that glutamate has been conver ...
... participation of glutamate is rather small, and there seems to be no stoichiometric relation to the accumulation of the organic acids. As isotachophoretic analysis did not reveal accumulations of metabolites other than acetate, succinate and propionate, we may conclude that glutamate has been conver ...
Preparing and Analyzing Expressed Sequence Tags (ESTs) Library
... intake of newborn lambs in the first days of their lives plays essential role in their healthy growth and reaching to ideal market weight [9]. The raisers are desired to breed very productive animal, so colostrums secretion is a crucially important stage for breeding. Because the compounds of colost ...
... intake of newborn lambs in the first days of their lives plays essential role in their healthy growth and reaching to ideal market weight [9]. The raisers are desired to breed very productive animal, so colostrums secretion is a crucially important stage for breeding. Because the compounds of colost ...
Metabolic implications of methionine excess. Effects of
... Under our experimental conditions, MAdT activity was influenced only by the amount of dietary methionine (free or contained in proteins) ; cortisol or insulin had no effect. Finkelstein (1967) found that hydrocortisone increased MAdT activity after ...
... Under our experimental conditions, MAdT activity was influenced only by the amount of dietary methionine (free or contained in proteins) ; cortisol or insulin had no effect. Finkelstein (1967) found that hydrocortisone increased MAdT activity after ...
Salinity Response in Chloroplasts: Insights from Gene
... Previous studies have characterized more than 53 salt-responsive genes encoding important chloroplast-localized proteins, which imply multiple vital pathways in chloroplasts in response to salt stress, such as thylakoid membrane organization, the modulation of photosystem II (PS II) activity, carbon ...
... Previous studies have characterized more than 53 salt-responsive genes encoding important chloroplast-localized proteins, which imply multiple vital pathways in chloroplasts in response to salt stress, such as thylakoid membrane organization, the modulation of photosystem II (PS II) activity, carbon ...
PHS 398/2590 (Rev. 06/09) - Cardiovascular Research Training
... G. William Wong is currently an Associate Professor in the Department of Physiology and Center for Metabolism and Obesity Research at The Johns Hopkins University School of Medicine. Dr. Wong received his Ph.D. degree from Harvard University, working on innate immunity, followed by post-doctoral tra ...
... G. William Wong is currently an Associate Professor in the Department of Physiology and Center for Metabolism and Obesity Research at The Johns Hopkins University School of Medicine. Dr. Wong received his Ph.D. degree from Harvard University, working on innate immunity, followed by post-doctoral tra ...
Converting Sugars to Biofuels: Ethanol and Beyond
... ethanol itself, growth inhibitors and toxic components from LCB-hydrolysates as well as other general stresses in ethanol-producing hosts [13]. Recently, extensive studies have been performed on ethanol production from LCB, and the advances are well summarized in a recent review [1]. Co-utilization ...
... ethanol itself, growth inhibitors and toxic components from LCB-hydrolysates as well as other general stresses in ethanol-producing hosts [13]. Recently, extensive studies have been performed on ethanol production from LCB, and the advances are well summarized in a recent review [1]. Co-utilization ...
The symbiotic ion channel homolog DMI1 is localized in the nuclear
... contains two close homologs of DMI1, CASTOR and POLLUX, both of which are required for infection and nodule development (Imaizumi-Anraku et al., 2005). In a similar manner as dmi1 mutants, castor and pollux mutants undergo root hair depolarization, but do not respond to Nod factors with nuclear calc ...
... contains two close homologs of DMI1, CASTOR and POLLUX, both of which are required for infection and nodule development (Imaizumi-Anraku et al., 2005). In a similar manner as dmi1 mutants, castor and pollux mutants undergo root hair depolarization, but do not respond to Nod factors with nuclear calc ...
here! - pst
... number of glycosamine glycan chains are bound. Components of glycose amine glycans comprise disaccharides with negatively charged sulfate and carboxyl groups, which determine the charge density of the extracellular cartilage matrix. These negative charges are neutralised by sodium ions of the extrac ...
... number of glycosamine glycan chains are bound. Components of glycose amine glycans comprise disaccharides with negatively charged sulfate and carboxyl groups, which determine the charge density of the extracellular cartilage matrix. These negative charges are neutralised by sodium ions of the extrac ...
Morris H. Aprison
... teachers. I quickly enrolled in the master's program in the Physics Department at UW, and in 1949 I received my M.S. degree. Since so many students were accepted into the physics graduate program after they had worked on the atomic bomb project in Chicago, "thesis" space for them was at a premium. H ...
... teachers. I quickly enrolled in the master's program in the Physics Department at UW, and in 1949 I received my M.S. degree. Since so many students were accepted into the physics graduate program after they had worked on the atomic bomb project in Chicago, "thesis" space for them was at a premium. H ...
mTOR complex 2 (mTORC2) controls hydrophobic motif
... kinase C) family of protein kinases and is activated by agonists including growth factors. SGK1 regulates diverse effects of extracellular agonists by phosphorylating regulatory proteins that control cellular processes such as ion transport and growth. Like other AGC family kinases, activation of SG ...
... kinase C) family of protein kinases and is activated by agonists including growth factors. SGK1 regulates diverse effects of extracellular agonists by phosphorylating regulatory proteins that control cellular processes such as ion transport and growth. Like other AGC family kinases, activation of SG ...
Sensitivity of plant mitochondrial terminal oxidases to the lipid
... Cox and Aox (alternative oxidase). Electron transport through the two pathways is regulated by the redox state of the ubiquinone pool and the activation status of the Aox protein, with the cytochrome pathway being the ‘default’ more active pathway [12]. Aox in its reduced dimeric form, accepts elect ...
... Cox and Aox (alternative oxidase). Electron transport through the two pathways is regulated by the redox state of the ubiquinone pool and the activation status of the Aox protein, with the cytochrome pathway being the ‘default’ more active pathway [12]. Aox in its reduced dimeric form, accepts elect ...
ppt - Chair of Computational Biology
... variation in their individual constituents and pathways, these metabolic networks have the same topological scaling properties and show striking similarities to the inherent organization of complex non-biological systems. This may indicate that metabolic organization is not only identical for all li ...
... variation in their individual constituents and pathways, these metabolic networks have the same topological scaling properties and show striking similarities to the inherent organization of complex non-biological systems. This may indicate that metabolic organization is not only identical for all li ...
Åpne - Munin
... The exposure to pollutants is a serious and increasing health problem, which has been associated with increased morbidity and mortality among people. Many toxic compounds can accumulate in the environment and evidence suggests that most people have varying degrees of toxic compounds within their bod ...
... The exposure to pollutants is a serious and increasing health problem, which has been associated with increased morbidity and mortality among people. Many toxic compounds can accumulate in the environment and evidence suggests that most people have varying degrees of toxic compounds within their bod ...
PDF
... phosphatase detected after electrophoresis is reduced or absent. It is known that alkaline phosphatase plays a major role in bone formation, as well as in mucoprotein and fibrous protein synthesis (Simkiss, 1964). The enzyme has also been implicated in determining the specificity of certain blood gr ...
... phosphatase detected after electrophoresis is reduced or absent. It is known that alkaline phosphatase plays a major role in bone formation, as well as in mucoprotein and fibrous protein synthesis (Simkiss, 1964). The enzyme has also been implicated in determining the specificity of certain blood gr ...
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... The studies of PrP indicate that there are two distinct strains of transmissible mink encephalopathy prions, ...
... The studies of PrP indicate that there are two distinct strains of transmissible mink encephalopathy prions, ...
Antifolding activity of hsp60 couples protein import into the
... reticulocyte lysate were added to isolated yeast mitochondria (Figure 2). Authentic precytochrome b2 was imported and processed in two steps to the intermediate and mature forms. Most of the mature-sized protein generated was in the intermembrane space and thus protected against externally added pro ...
... reticulocyte lysate were added to isolated yeast mitochondria (Figure 2). Authentic precytochrome b2 was imported and processed in two steps to the intermediate and mature forms. Most of the mature-sized protein generated was in the intermembrane space and thus protected against externally added pro ...
New insight into plant intramembrane proteases
... According to this classification, the PARL type proteases are characterized by the presence of 6 α-helixes forming the core of the catalytic domain and an additional α-helix at their N-terminus. The group of proteins containing, beside the 6 α-helixes of the core, an additional α-helix at their C-te ...
... According to this classification, the PARL type proteases are characterized by the presence of 6 α-helixes forming the core of the catalytic domain and an additional α-helix at their N-terminus. The group of proteins containing, beside the 6 α-helixes of the core, an additional α-helix at their C-te ...
Paracrine signalling
Paracrine signaling is a form of cell-cell communication in which a cell produces a signal to induce changes in nearby cells, altering the behavior or differentiation of those cells. Signaling molecules known as paracrine factors diffuse over a relatively short distance (local action), as opposed to endocrine factors (hormones which travel considerably longer distances via the circulatory system), juxtacrine interactions, and autocrine signaling. Cells that produce paracrine factors secrete them into the immediate extracellular environment. Factors then travel to nearby cells in which the gradient of factor received determines the outcome. However, the exact distance that paracrine factors can travel is not certain.Although paracrine signaling elicits a diverse array of responses in the induced cells, most paracrine factors utilize a relatively streamlined set of receptors and pathways. In fact, different organs in the body -even between different species - are known to utilize a similar sets of paracrine factors in differential development. The highly conserved receptors and pathways can be organized into four major families based on similar structures: Fibroblast growth factor (FGF) family, Hedgehog family, Wnt family, and TGF-β superfamily. Binding of a paracrine factor to its respective receptor initiates signal transduction cascades, eliciting different responses.