Role of lectins (and rhizobial exopolysaccharides) in legume
... A larger question is how the bacteria are engulfed once they make contact with the root hair. The lectins used to formulate the lectin recognition hypothesis have no transmembrane domains that could connect directly to components of a signal transduction cascade. Legume seed lectins are usually holo ...
... A larger question is how the bacteria are engulfed once they make contact with the root hair. The lectins used to formulate the lectin recognition hypothesis have no transmembrane domains that could connect directly to components of a signal transduction cascade. Legume seed lectins are usually holo ...
Current Opinion in Plant Biology
... A larger question is how the bacteria are engulfed once they make contact with the root hair. The lectins used to formulate the lectin recognition hypothesis have no transmembrane domains that could connect directly to components of a signal transduction cascade. Legume seed lectins are usually holo ...
... A larger question is how the bacteria are engulfed once they make contact with the root hair. The lectins used to formulate the lectin recognition hypothesis have no transmembrane domains that could connect directly to components of a signal transduction cascade. Legume seed lectins are usually holo ...
From Structure to Function
... residues in reasonable proximity, yet provide microenvironments around each one that make it unfavorable for them to exchange a proton. This occurs in many enzyme active sites; for example, in aspartate aminotransferase, a lysine and an aspartic acid are both involved in binding the pyridoxal phosph ...
... residues in reasonable proximity, yet provide microenvironments around each one that make it unfavorable for them to exchange a proton. This occurs in many enzyme active sites; for example, in aspartate aminotransferase, a lysine and an aspartic acid are both involved in binding the pyridoxal phosph ...
Regulation of 7-Dehydrocholesterol Reductase by Vitamin D3
... Cholesterol is the precursor molecule in the synthetic pathways of steroid hormones and bile acids. The rate-limiting step of steroid hormone biosynthesis occurs in mitochondria where free cholesterol (from the hydrolysis of cholesterol esters) is converted to pregnenolone by cholesterol side-chain ...
... Cholesterol is the precursor molecule in the synthetic pathways of steroid hormones and bile acids. The rate-limiting step of steroid hormone biosynthesis occurs in mitochondria where free cholesterol (from the hydrolysis of cholesterol esters) is converted to pregnenolone by cholesterol side-chain ...
characterization of proteins from the cytoskeleton of giardia lamblia
... with tubulin; by selecting appropriate subunits from among tubulin species; or, after incorporation of common tubulin precursors, by allowing chemical modification of dimers in organelle- or site-specific patterns. Giardia is an endoparasitic flagellate with a number of microtubular organelles. Extr ...
... with tubulin; by selecting appropriate subunits from among tubulin species; or, after incorporation of common tubulin precursors, by allowing chemical modification of dimers in organelle- or site-specific patterns. Giardia is an endoparasitic flagellate with a number of microtubular organelles. Extr ...
Fixation
... electron microscopy, fixes well at 4oC, and gives best overall cytoplasmic and nuclear detail. • However it is not ideal for immunohistochemistry staining • Some fixation protocols call for a combination of formaldehyde and glutaraldehyde, so that their ...
... electron microscopy, fixes well at 4oC, and gives best overall cytoplasmic and nuclear detail. • However it is not ideal for immunohistochemistry staining • Some fixation protocols call for a combination of formaldehyde and glutaraldehyde, so that their ...
A PEST-like Sequence in the N-Terminal Cytoplasmic Domain of
... Mutation analysis was used to define the ubiquitination and endocytosis targeting signals in several yeast membrane proteins. These include the SINNDAKSS motif in the C-terminal cytoplasmic domain of the Ste2 R-factor receptor (6, 7). Deletion analysis demonstrated that the middlespanning linker reg ...
... Mutation analysis was used to define the ubiquitination and endocytosis targeting signals in several yeast membrane proteins. These include the SINNDAKSS motif in the C-terminal cytoplasmic domain of the Ste2 R-factor receptor (6, 7). Deletion analysis demonstrated that the middlespanning linker reg ...
Fixation
... electron microscopy, fixes well at 4oC, and gives best overall cytoplasmic and nuclear detail. • However it is not ideal for immunohistochemistry staining • Some fixation protocols call for a combination of formaldehyde and glutaraldehyde, so that their ...
... electron microscopy, fixes well at 4oC, and gives best overall cytoplasmic and nuclear detail. • However it is not ideal for immunohistochemistry staining • Some fixation protocols call for a combination of formaldehyde and glutaraldehyde, so that their ...
The semi-phosphorylative Entner–Doudoroff pathway in
... galactonate γ -lactone by incubation in 1 M NaOH for 1 h (4 M stock solution) and subsequent dilution in 50 mM Hepes/KOH (pH 7.0, 70 ◦C) as described in [31]. The activity of the T. tenax and S. solfataricus KDG kinases was determined at 70 and 60 ◦C respectively using a continuous assay. The phosph ...
... galactonate γ -lactone by incubation in 1 M NaOH for 1 h (4 M stock solution) and subsequent dilution in 50 mM Hepes/KOH (pH 7.0, 70 ◦C) as described in [31]. The activity of the T. tenax and S. solfataricus KDG kinases was determined at 70 and 60 ◦C respectively using a continuous assay. The phosph ...
DNA Specificity of the Bicoid Activator Protein Is Determined by
... at position 1 and still activated a Bicoid site target. LexABicoid mutant A5 contained a Lys+Ala substitution at position 5 and also activated a Bicoid site target but at reduced levels. However, activation by this mutant was also reduced using a LexA site target, perhaps because the protein is less ...
... at position 1 and still activated a Bicoid site target. LexABicoid mutant A5 contained a Lys+Ala substitution at position 5 and also activated a Bicoid site target but at reduced levels. However, activation by this mutant was also reduced using a LexA site target, perhaps because the protein is less ...
Polypeptide Composition of Envelopes of Spinach Chloroplasts
... Separation of outer and inner envelope membranes— For preparation of chloroplast envelopes, two procedures have been adopted to rupture intact chloroplasts; freezing and thawing (Cline et al. 1981) and mechanical homogenization (Block et al. 1983). In the present study, we used the freezing and thaw ...
... Separation of outer and inner envelope membranes— For preparation of chloroplast envelopes, two procedures have been adopted to rupture intact chloroplasts; freezing and thawing (Cline et al. 1981) and mechanical homogenization (Block et al. 1983). In the present study, we used the freezing and thaw ...
Denise Lim, Instructor Study Guide Bio 5 - Human Physiology
... 5. What is a solution? What is a solvent? How do polar (water) and nonpolar (fat) solvents differ? What is a solute? What is an acid? What is a base? How do buffers prevent fluctuations in pH? Know, in particular, the bicarbonate buffer discussed in lab. 6. What are some common solutes found in the ...
... 5. What is a solution? What is a solvent? How do polar (water) and nonpolar (fat) solvents differ? What is a solute? What is an acid? What is a base? How do buffers prevent fluctuations in pH? Know, in particular, the bicarbonate buffer discussed in lab. 6. What are some common solutes found in the ...
Chapter 1: Introduction and Homeostasis
... 5. What is a solution? What is a solvent? How do polar (water) and nonpolar (fat) solvents differ? What is a solute? What is an acid? What is a base? How do buffers prevent fluctuations in pH? Know, in particular, the bicarbonate buffer discussed in lab. 6. What are some common solutes found in the ...
... 5. What is a solution? What is a solvent? How do polar (water) and nonpolar (fat) solvents differ? What is a solute? What is an acid? What is a base? How do buffers prevent fluctuations in pH? Know, in particular, the bicarbonate buffer discussed in lab. 6. What are some common solutes found in the ...
A fluorophore ligase for site-specific protein labeling inside living cells
... explore the biology of nuclear actin. Cytosolic β-actin has a wellrecognized role in controlling cell migration and morphology, but biochemical studies have also suggested several roles for β-actin in the nucleus, including involvement in transcriptional regulation (24) and chromatin remodeling (25) ...
... explore the biology of nuclear actin. Cytosolic β-actin has a wellrecognized role in controlling cell migration and morphology, but biochemical studies have also suggested several roles for β-actin in the nucleus, including involvement in transcriptional regulation (24) and chromatin remodeling (25) ...
Determination of De Novo Synthesized Amino Acids in Cellular
... proline was largely decreased. Apparently, hypoosmotic conditions stimulate the synthesis of proteins or peptides with a high proline content. The results show that already after 2 h of incubation with [U-13C]glucose there is a pronounced flux of 13C label into the cellular proteins, which is usuall ...
... proline was largely decreased. Apparently, hypoosmotic conditions stimulate the synthesis of proteins or peptides with a high proline content. The results show that already after 2 h of incubation with [U-13C]glucose there is a pronounced flux of 13C label into the cellular proteins, which is usuall ...
Anaerobic degradation of aromatic amino acids by
... quantitative real-time (qRT)-PCR analyses was extracted during exponential growth of F. placidus on phenylalanine, phenylacetate and acetate. In all cases, triplicate RNA samples were extracted. For extraction of RNA, cultures (100 ml in 156 ml serum bottles) were divided into 50 ml conical tubes (F ...
... quantitative real-time (qRT)-PCR analyses was extracted during exponential growth of F. placidus on phenylalanine, phenylacetate and acetate. In all cases, triplicate RNA samples were extracted. For extraction of RNA, cultures (100 ml in 156 ml serum bottles) were divided into 50 ml conical tubes (F ...
MORINGA OLEIFERA IN SILICO Research Article
... Method: Glucosaminyl (N-acetyl) transferase 1 (GCNT1), cellular Prostatic acid phosphatase (cPAP), HER-2 (Human Epidermal growth factor Receptor 2) and ERK (Extracellular Regulated Kinase) which usually require cPAP for their dephosphorylation and inactivation, were chosen as the drug targets. M. ol ...
... Method: Glucosaminyl (N-acetyl) transferase 1 (GCNT1), cellular Prostatic acid phosphatase (cPAP), HER-2 (Human Epidermal growth factor Receptor 2) and ERK (Extracellular Regulated Kinase) which usually require cPAP for their dephosphorylation and inactivation, were chosen as the drug targets. M. ol ...
Enhancement of Fibronectin Fibrillogenesis and Bone Formation by
... differentiation, and survival in cells. bFGF is a 16.5-kDa heparin binding growth factor that influences the proliferation and differentiation of various cell types in vitro (Gospodarowicz et al., 1987). The skeleton is an important target tissue for FGFs; these factors are involved in bone developm ...
... differentiation, and survival in cells. bFGF is a 16.5-kDa heparin binding growth factor that influences the proliferation and differentiation of various cell types in vitro (Gospodarowicz et al., 1987). The skeleton is an important target tissue for FGFs; these factors are involved in bone developm ...
Effect of microaerobiosis
... showed all characteristic changes in the amino acids specific to D1’ isoform (Mulo et al., 2009). The microaerobic conditions, achieved by bubbling argon gas to Synechococcus sp. PCC 7002 cells while maintaining all other growing parameters constant did not significantly change the function of the d ...
... showed all characteristic changes in the amino acids specific to D1’ isoform (Mulo et al., 2009). The microaerobic conditions, achieved by bubbling argon gas to Synechococcus sp. PCC 7002 cells while maintaining all other growing parameters constant did not significantly change the function of the d ...
Introduction
... Plant sterols and their saturated derivatives, stanols, are a group of cholesterol analogues with different side chain configurations. ...
... Plant sterols and their saturated derivatives, stanols, are a group of cholesterol analogues with different side chain configurations. ...
Protein Structure and Interactions
... The cytosolic eukaryotic chaperonin TRiC/CCT (for TCP1 ring complex or chaperonin-containing TCP1) has an important role for folding in the cell, as evidenced by its essentiality for life. Initially, this requirement was thought to be a consequence of an exclusive role in folding the cytoskeletal pr ...
... The cytosolic eukaryotic chaperonin TRiC/CCT (for TCP1 ring complex or chaperonin-containing TCP1) has an important role for folding in the cell, as evidenced by its essentiality for life. Initially, this requirement was thought to be a consequence of an exclusive role in folding the cytoskeletal pr ...
(De)regulation of key enzyme steps in the shikimate pathway and
... present in A. methanolica. DAHP synthase 1 (DS1) is a 160 kDa enzyme associated non-covalently with a dimeric CM protein, thus forming a heteromeric two-enzyme complex. The two enzyme activities can be separated by Q-Sepharose anion-exchange chromatography, yielding a dimeric CM protein with a fivef ...
... present in A. methanolica. DAHP synthase 1 (DS1) is a 160 kDa enzyme associated non-covalently with a dimeric CM protein, thus forming a heteromeric two-enzyme complex. The two enzyme activities can be separated by Q-Sepharose anion-exchange chromatography, yielding a dimeric CM protein with a fivef ...
Cell Biology - Models NF
... The are many upstream regulators described in the literature. How each upstream kinase fits in, is unclear, especially in regards to specific signaling. IKK regulation appears to be a point of convergence for a number of different signaling pathways. Some of the upstream players include: ...
... The are many upstream regulators described in the literature. How each upstream kinase fits in, is unclear, especially in regards to specific signaling. IKK regulation appears to be a point of convergence for a number of different signaling pathways. Some of the upstream players include: ...
Iron-sulfur proteins and the role of iron in the gene expression of
... 2. FeS clusters and FeS proteins Proteins that contain iron-sulfur (FeS) clusters as a prosthetic group are widely utilised in all living organisms for a variety of cellular processes. Inorganic FeS metal compounds were already present in hydrothermal vents in the anaerobic atmosphere of the ancien ...
... 2. FeS clusters and FeS proteins Proteins that contain iron-sulfur (FeS) clusters as a prosthetic group are widely utilised in all living organisms for a variety of cellular processes. Inorganic FeS metal compounds were already present in hydrothermal vents in the anaerobic atmosphere of the ancien ...
Metabolic processes of Methanococcus maripaludis and potential
... [15]. Ether lipids recovered from M. maripaludis mainly include glycolipids [14.2 mg ether lipid/g dry cell weight (DCW)] and polar lipids (0.4 mg ether lipid/g DCW) [16]. The motility in methanococci is due to the presence of flagella, however strong attachments by M. maripaludis to various surfac ...
... [15]. Ether lipids recovered from M. maripaludis mainly include glycolipids [14.2 mg ether lipid/g dry cell weight (DCW)] and polar lipids (0.4 mg ether lipid/g DCW) [16]. The motility in methanococci is due to the presence of flagella, however strong attachments by M. maripaludis to various surfac ...
Paracrine signalling
Paracrine signaling is a form of cell-cell communication in which a cell produces a signal to induce changes in nearby cells, altering the behavior or differentiation of those cells. Signaling molecules known as paracrine factors diffuse over a relatively short distance (local action), as opposed to endocrine factors (hormones which travel considerably longer distances via the circulatory system), juxtacrine interactions, and autocrine signaling. Cells that produce paracrine factors secrete them into the immediate extracellular environment. Factors then travel to nearby cells in which the gradient of factor received determines the outcome. However, the exact distance that paracrine factors can travel is not certain.Although paracrine signaling elicits a diverse array of responses in the induced cells, most paracrine factors utilize a relatively streamlined set of receptors and pathways. In fact, different organs in the body -even between different species - are known to utilize a similar sets of paracrine factors in differential development. The highly conserved receptors and pathways can be organized into four major families based on similar structures: Fibroblast growth factor (FGF) family, Hedgehog family, Wnt family, and TGF-β superfamily. Binding of a paracrine factor to its respective receptor initiates signal transduction cascades, eliciting different responses.