Theory and practice of size exclusion chromatography for
... immunogenicity [12,13]. In recent studies, cytotoxic effects have also been observed with several biotherapeutic proteins due to significant denaturation or chemical alterations of the native protein [8,9,14]. In this context, the characterization of protein modifications requires a number of analytic ...
... immunogenicity [12,13]. In recent studies, cytotoxic effects have also been observed with several biotherapeutic proteins due to significant denaturation or chemical alterations of the native protein [8,9,14]. In this context, the characterization of protein modifications requires a number of analytic ...
Biogenesis of the Protein Storage Vacuole Crystalloid
... (for DIP; Culianez-Macia and Martin, 1993) and are predominantly located in the cytoplasm. As seed development proceeds, these organelles are taken up by PSVs and aggregate such that they form the crystalloid in mature seeds. These organelles also represent an intermediate for vesicular traffic from ...
... (for DIP; Culianez-Macia and Martin, 1993) and are predominantly located in the cytoplasm. As seed development proceeds, these organelles are taken up by PSVs and aggregate such that they form the crystalloid in mature seeds. These organelles also represent an intermediate for vesicular traffic from ...
Mitochondrial stress causes increased succination of proteins in
... (1,1-dimethylbiguanide hydrochloride)], for a further 2–8 days (maturation period); during this time lipid droplets accumulated in the cytoplasm. Preliminary experiments using L-glucose as an osmotic control indicated that it had no effect on protein succination (results not shown). Culture medium w ...
... (1,1-dimethylbiguanide hydrochloride)], for a further 2–8 days (maturation period); during this time lipid droplets accumulated in the cytoplasm. Preliminary experiments using L-glucose as an osmotic control indicated that it had no effect on protein succination (results not shown). Culture medium w ...
Cell-Free (In Vitro) Protein Expression
... Western blotting detection using primary antibody to the expressed protein ...
... Western blotting detection using primary antibody to the expressed protein ...
Identification of the tRNA-binding Protein Arc1p as a Novel Target of
... the S. cerevisiae/E. coli shuttle vectors YCplac33 (14), p414MET25 (15), pVT-100U (16), pTRC-HISA (Invitrogen) and the E. coli plasmid pQE70 (Qiagen) served as recipients. Plasmid pFA6-HIS3MX6 (17) and pFA6KanMX (18) were used for PCR amplification of HIS5 and KanMX insertion cassettes, respectively ...
... the S. cerevisiae/E. coli shuttle vectors YCplac33 (14), p414MET25 (15), pVT-100U (16), pTRC-HISA (Invitrogen) and the E. coli plasmid pQE70 (Qiagen) served as recipients. Plasmid pFA6-HIS3MX6 (17) and pFA6KanMX (18) were used for PCR amplification of HIS5 and KanMX insertion cassettes, respectively ...
SFE1, a Constituent of the Fertilization Envelope
... found in several of the cysteine-rich repeats (data not shown). Both the monoclonal and polyclonal antibodies yield the same results. That is, SFE1 was present specifi- ...
... found in several of the cysteine-rich repeats (data not shown). Both the monoclonal and polyclonal antibodies yield the same results. That is, SFE1 was present specifi- ...
Chapter 2
... imagine an almost limitless variety of peptides. For example, there are 2050 or slightly more than 1.12 ⫻ 1065 possible sequences for polypeptides with just 50 amino acid residues. ...
... imagine an almost limitless variety of peptides. For example, there are 2050 or slightly more than 1.12 ⫻ 1065 possible sequences for polypeptides with just 50 amino acid residues. ...
Proteomics Analysis of Immunoprecipitated Proteins Associated with
... be largely responsible for the difference in activity. A putative PEST sequence was also found between residues 415 and 438. In this study, we attempted to determine potential binding partners to Cot and their region of association by separating proteins that were pulled-down with various Cot constr ...
... be largely responsible for the difference in activity. A putative PEST sequence was also found between residues 415 and 438. In this study, we attempted to determine potential binding partners to Cot and their region of association by separating proteins that were pulled-down with various Cot constr ...
Investigation of a Zα-like Peptide Motif in Koi Herpesvirus
... settling time of 0.330 and 3 scans per sample at 25 ◦ C. A 500 µL cuvette was filled with 295 µL of CD buffer (10 mM HEPES, pH 7.4, 20 mM KF, and 0.1 mM EDTA) as a baseline. Then, 5.1 µL of poly(dGdC) DNA (purchased from GE Healthcare/Amersham Biosciences) was added and run. Protein (KHV WT, Y84A, N ...
... settling time of 0.330 and 3 scans per sample at 25 ◦ C. A 500 µL cuvette was filled with 295 µL of CD buffer (10 mM HEPES, pH 7.4, 20 mM KF, and 0.1 mM EDTA) as a baseline. Then, 5.1 µL of poly(dGdC) DNA (purchased from GE Healthcare/Amersham Biosciences) was added and run. Protein (KHV WT, Y84A, N ...
2.5 | Four Types of Biological Molecules
... too does the number of asymmetric carbon atoms and, consequently, the number of stereoisomers. Aldotetroses have two asymmetric carbons and thus can exist in four different configurations (Figure 2.14). Similarly, there are 8 different aldopentoses and 16 different aldohexoses. The designation of ea ...
... too does the number of asymmetric carbon atoms and, consequently, the number of stereoisomers. Aldotetroses have two asymmetric carbons and thus can exist in four different configurations (Figure 2.14). Similarly, there are 8 different aldopentoses and 16 different aldohexoses. The designation of ea ...
Protein structure
... Many of the images in this powerpoint presentation are from Bioinformatics and Functional Genomics by Jonathan Pevsner (ISBN 0-471-21004-8). Copyright © 2003 by John Wiley & Sons, Inc. These images and materials may not be used without permission from the publisher. We welcome instructors to use the ...
... Many of the images in this powerpoint presentation are from Bioinformatics and Functional Genomics by Jonathan Pevsner (ISBN 0-471-21004-8). Copyright © 2003 by John Wiley & Sons, Inc. These images and materials may not be used without permission from the publisher. We welcome instructors to use the ...
IOSR Journal of Applied Chemistry (IOSR-JAC) e-ISSN: 2278-5736.
... Molecular docking is a method to predict the preferred orientation of one molecule to a second when bound to each other to form a stable complex. Computers and programs (software‟s) are used to predict or simulate the possible reaction (and interactions) between two molecules based on their three di ...
... Molecular docking is a method to predict the preferred orientation of one molecule to a second when bound to each other to form a stable complex. Computers and programs (software‟s) are used to predict or simulate the possible reaction (and interactions) between two molecules based on their three di ...
Translation
... complex such that the tRNAfMet interacts via base pairing with the mRNA initiation codon (AUG). A region of mRNA upstream of the initiation codon, the Shine-Dalgarno sequence, base pairs with the 3' end of the 16S rRNA. This positions the 30S ribosomal subunit in relation to the initiation codon. ...
... complex such that the tRNAfMet interacts via base pairing with the mRNA initiation codon (AUG). A region of mRNA upstream of the initiation codon, the Shine-Dalgarno sequence, base pairs with the 3' end of the 16S rRNA. This positions the 30S ribosomal subunit in relation to the initiation codon. ...
Methods to Make Homogenous Antibody Drug Conjugates
... introduced as site-specific chemical moieties through site directed mutagenesis. Due to the highly reactive nature of cysteine as a nucleophile, the free thiols can then be selectively modified through the use of maleimides or other electrophilic groups. Engineering free cysteines into antibodies fo ...
... introduced as site-specific chemical moieties through site directed mutagenesis. Due to the highly reactive nature of cysteine as a nucleophile, the free thiols can then be selectively modified through the use of maleimides or other electrophilic groups. Engineering free cysteines into antibodies fo ...
Prediction of mitochondrial proteins of malaria parasite
... protein provides more information than composition of whole protein (Kumar et al. 2006). Based on this observation, methods have been developed using split amino acid composition (Verma et al. 2008). In SAAC, first sequence is divided in parts then composition of each part is computed separately and ...
... protein provides more information than composition of whole protein (Kumar et al. 2006). Based on this observation, methods have been developed using split amino acid composition (Verma et al. 2008). In SAAC, first sequence is divided in parts then composition of each part is computed separately and ...
Biogenesis of the Protein Storage Vacuole Crystalloid
... (for DIP; Culianez-Macia and Martin, 1993) and are predominantly located in the cytoplasm. As seed development proceeds, these organelles are taken up by PSVs and aggregate such that they form the crystalloid in mature seeds. These organelles also represent an intermediate for vesicular traffic from ...
... (for DIP; Culianez-Macia and Martin, 1993) and are predominantly located in the cytoplasm. As seed development proceeds, these organelles are taken up by PSVs and aggregate such that they form the crystalloid in mature seeds. These organelles also represent an intermediate for vesicular traffic from ...
PC235: 2008 Lecture 5 Quantitation
... •Count the number of peptides identified from a protein in each sample. •Do you count repeat identifications of the same peptide? •Not accurate at quantifying magnitude of change, but can be used to determine if there is a difference. •In general, need a spectral count difference of about 4 peptides ...
... •Count the number of peptides identified from a protein in each sample. •Do you count repeat identifications of the same peptide? •Not accurate at quantifying magnitude of change, but can be used to determine if there is a difference. •In general, need a spectral count difference of about 4 peptides ...
Abstract
... properties. Of all inorganic cofactors, transition metal ions play a unique role in proteins. Among all of the transition metal ions present in all domains of life, zinc (formally Zn(II)) is one of the most widespread, reflecting the utilization of Zn(II) by proteins for a wide variety of biological ...
... properties. Of all inorganic cofactors, transition metal ions play a unique role in proteins. Among all of the transition metal ions present in all domains of life, zinc (formally Zn(II)) is one of the most widespread, reflecting the utilization of Zn(II) by proteins for a wide variety of biological ...
Section 5.3: Proteins
... sterically reasonable values of the angles f and y. The shaded regions indicate particularly favorable values of these angles. Dots in purple indicate actual angles measured for 1000 residues (excluding glycine, for which a wider range of angles is permitted) in eight proteins. The lines running acr ...
... sterically reasonable values of the angles f and y. The shaded regions indicate particularly favorable values of these angles. Dots in purple indicate actual angles measured for 1000 residues (excluding glycine, for which a wider range of angles is permitted) in eight proteins. The lines running acr ...
Ashley, CT, Wilkinson, KD, Reines, D and Warren, ST: FMR1 protein: Conserved RNP family domains and selective RNA binding. Science 262:563-566 (1993).
... RNA actsas a better ligand than nonbiotinylated RNA or that there are two RNA binding sites per protein molecule. The latter possibilitywould reducethe apparent binding constant because biotinylated RNA bound to either or both sites would result in captureof the FMRPmolecule by streptavidin. Because ...
... RNA actsas a better ligand than nonbiotinylated RNA or that there are two RNA binding sites per protein molecule. The latter possibilitywould reducethe apparent binding constant because biotinylated RNA bound to either or both sites would result in captureof the FMRPmolecule by streptavidin. Because ...
better health is only a glass awhey
... greatest health benefits. The whey is also processed at a lower temperature than normal, so that amino acids vital for the production of antioxidants are not damaged by high heat. These factors make Joe’s product unique, as it tailor made to help the body fight the ageing process. “Vitality Whey is ...
... greatest health benefits. The whey is also processed at a lower temperature than normal, so that amino acids vital for the production of antioxidants are not damaged by high heat. These factors make Joe’s product unique, as it tailor made to help the body fight the ageing process. “Vitality Whey is ...
Assembly and disassembly of the influenza C matrix protein layer on
... addressed by this work. • Can M1C bind to uncharged lipids? A recent article claims that M1 from influenza A does not require any negatively charged lipids in a bilayer to bind to it [9]. Should it become apparent that M1C is not able to bind to uncharged lipids this would be an interesting distinct ...
... addressed by this work. • Can M1C bind to uncharged lipids? A recent article claims that M1 from influenza A does not require any negatively charged lipids in a bilayer to bind to it [9]. Should it become apparent that M1C is not able to bind to uncharged lipids this would be an interesting distinct ...
A Proteome Reference Map and Proteomic Analysis
... against the database of B. longum NCC2705 (Version 050126, 1727 sequences) and NCBInr (Version 050623, 2,564,994 sequences); the research results were checked using Mascot with free access on the Internet (www.matrixscience.com). Monoisotopic masses were used to search the databases, allowing a pept ...
... against the database of B. longum NCC2705 (Version 050126, 1727 sequences) and NCBInr (Version 050623, 2,564,994 sequences); the research results were checked using Mascot with free access on the Internet (www.matrixscience.com). Monoisotopic masses were used to search the databases, allowing a pept ...
Animal Dicer and plant Dicer-like proteins
... et al., 2012a). This offers significant potential to influence the nuclease activity of the enzyme; yet the functions of both domains appear highly diverse. The helicase domain has been shown to have an inhibitory effect on dsRNA processing by human Dicer (Ma et al., 2008). This domain is also cruci ...
... et al., 2012a). This offers significant potential to influence the nuclease activity of the enzyme; yet the functions of both domains appear highly diverse. The helicase domain has been shown to have an inhibitory effect on dsRNA processing by human Dicer (Ma et al., 2008). This domain is also cruci ...
Protein purification
Protein purification is a series of processes intended to isolate one or a few proteins from a complex mixture, usually cells, tissues or whole organisms. Protein purification is vital for the characterization of the function, structure and interactions of the protein of interest. The purification process may separate the protein and non-protein parts of the mixture, and finally separate the desired protein from all other proteins. Separation of one protein from all others is typically the most laborious aspect of protein purification. Separation steps usually exploit differences in protein size, physico-chemical properties, binding affinity and biological activity. The pure result may be termed protein isolate.The methods used in protein purification can roughly be divided into analytical and preparative methods. The distinction is not exact, but the deciding factor is the amount of protein that can practically be purified with that method. Analytical methods aim to detect and identify a protein in a mixture, whereas preparative methods aim to produce large quantities of the protein for other purposes, such as structural biology or industrial use. In general, the preparative methods can be used in analytical applications, but not the other way around.