In vitro gastrointestinal digestion study of a novel bio-tofu
... by means of lactic acid bacteria (LAB) incorporated with microbial transglutaminase (MTGase) instead of bittern as coagulant. MTGase (EC 2.3.2.13) is an enzyme that catalyzes the transfer reaction between many proteins by crosslinking of the amino acid residues of protein bound glutamine and lysine ...
... by means of lactic acid bacteria (LAB) incorporated with microbial transglutaminase (MTGase) instead of bittern as coagulant. MTGase (EC 2.3.2.13) is an enzyme that catalyzes the transfer reaction between many proteins by crosslinking of the amino acid residues of protein bound glutamine and lysine ...
1 This exam consists of 5 pages and 15
... iii) What is the charge on this dipeptide, at pH=7. Briefly justify your answer with reference to individual pKa values (4 pts). Since the pH is 2 units away from all pKa values, the two amino groups, with a pKa of 9 will be fully protonated with a charge of +2. The mainchain carbonyl will be fully ...
... iii) What is the charge on this dipeptide, at pH=7. Briefly justify your answer with reference to individual pKa values (4 pts). Since the pH is 2 units away from all pKa values, the two amino groups, with a pKa of 9 will be fully protonated with a charge of +2. The mainchain carbonyl will be fully ...
Molecular Interaction of PICKI and ATXN3
... SCA3 is one of the nine known polyQ diseases which would be inherited in an autosomal dominantly. Exact disease mechanism of SCA3 is not fully understood and there is currently no known therapy to affect disease progression. SCA3 is solely caused by CAG expansion of ATXN3, a deubiquitinase with a un ...
... SCA3 is one of the nine known polyQ diseases which would be inherited in an autosomal dominantly. Exact disease mechanism of SCA3 is not fully understood and there is currently no known therapy to affect disease progression. SCA3 is solely caused by CAG expansion of ATXN3, a deubiquitinase with a un ...
Enzyme changes in malnutrition - Journal of Clinical Pathology
... It is interesting that, when rickets is accompanied by protein deficiency, alkaline phosphatase levels are low instead of high; in fact, the florid signs of rickets do not appear until protein is given and the child begins to grow. The low levels of amylase and lipase in serum are accompanied by a r ...
... It is interesting that, when rickets is accompanied by protein deficiency, alkaline phosphatase levels are low instead of high; in fact, the florid signs of rickets do not appear until protein is given and the child begins to grow. The low levels of amylase and lipase in serum are accompanied by a r ...
Evaluation of novel affinity bio-beads for use in the production of
... 1. The capability of two novel affinity resins (Polybind-Z and Polybind-L) produced by bacteria and incorporated with specific ligands for IgG, IgA and IgM was examined for the capability of removing these residual proteins an albumin intermediate containing these residual proteins. 2. Polybind-Z ex ...
... 1. The capability of two novel affinity resins (Polybind-Z and Polybind-L) produced by bacteria and incorporated with specific ligands for IgG, IgA and IgM was examined for the capability of removing these residual proteins an albumin intermediate containing these residual proteins. 2. Polybind-Z ex ...
Adhesins and invasins of pathogenic bacteria: a
... E-cadherin. In the InlA′/hEC1 complex, the C-termini of both molecules point in opposite directions. This is biologically significant, as InlA and E-cadherin are attached to the cell surfaces by their C-termini. The observed orientation puts the bacterium and the host cell on opposite sides of the r ...
... E-cadherin. In the InlA′/hEC1 complex, the C-termini of both molecules point in opposite directions. This is biologically significant, as InlA and E-cadherin are attached to the cell surfaces by their C-termini. The observed orientation puts the bacterium and the host cell on opposite sides of the r ...
Protein Kinase A Regulatory Subunit Interacts with P
... whether TcPKAc would interact with these P-type ATPases in the yeast two-hybrid system and found that they could not interact in this system (data not shown). This indicates that interactions of TcPKAr and these P-type ATPases are specific. One of the candidates turned out to be a Na+-ATPase mediati ...
... whether TcPKAc would interact with these P-type ATPases in the yeast two-hybrid system and found that they could not interact in this system (data not shown). This indicates that interactions of TcPKAr and these P-type ATPases are specific. One of the candidates turned out to be a Na+-ATPase mediati ...
A1990DV41100002
... processes. But Kahlson had found exceptions, rapidly growing tissues, such as regenerating rat liver, in which histidine decarboxylase activity was not altered. I wondered if some diamine other than histamine, perhaps putrescine or cadaverine, might be implicated in these cases. One could readily me ...
... processes. But Kahlson had found exceptions, rapidly growing tissues, such as regenerating rat liver, in which histidine decarboxylase activity was not altered. I wondered if some diamine other than histamine, perhaps putrescine or cadaverine, might be implicated in these cases. One could readily me ...
WHOLE BUCKWHEAT
... A native to Russia, buckwheat is another of the super grains. It is not an actual grain, however, but an herb belonging to the same family as sorrel and rhubarb. Whole buckwheat groats are the raw kernels with their inedible black shells removed. Whole grains are either white (unroasted) or brown (r ...
... A native to Russia, buckwheat is another of the super grains. It is not an actual grain, however, but an herb belonging to the same family as sorrel and rhubarb. Whole buckwheat groats are the raw kernels with their inedible black shells removed. Whole grains are either white (unroasted) or brown (r ...
A new subfamily of fungal subtilases: structural and functional
... in the regulation of the ligninolytic activities of this fungus. Recently, purification and characterization of the most abundant P. ostreatus extracellular protease (PoSl) have been reported. The sequence of the posl gene and of the corresponding cDNA has been determined, allowing the identificatio ...
... in the regulation of the ligninolytic activities of this fungus. Recently, purification and characterization of the most abundant P. ostreatus extracellular protease (PoSl) have been reported. The sequence of the posl gene and of the corresponding cDNA has been determined, allowing the identificatio ...
Identification of a Protein with Antioxidant Activity that is Important for
... reagent, MPB (N'-(3-maleidylpropionyl)) biotin and SDS-PAGE separation. Initial Coomassie Blue staining showed that each sample of beer contained 2 major protein bands at 43 kDa and 10 kDa and they were in similar concentrations (Figure 2a). Free thiol labelling revealed that only the 10 kDa protein ...
... reagent, MPB (N'-(3-maleidylpropionyl)) biotin and SDS-PAGE separation. Initial Coomassie Blue staining showed that each sample of beer contained 2 major protein bands at 43 kDa and 10 kDa and they were in similar concentrations (Figure 2a). Free thiol labelling revealed that only the 10 kDa protein ...
as PDF
... matrix synthesis and apoptosis. They play major roles during prenatal development and postnatal growth, remodeling, and maintenance of a variety of tissues and organs. In accordance with their role in regulating vital biological processes, the amino-acid sequences of homologous TGF-β proteins from d ...
... matrix synthesis and apoptosis. They play major roles during prenatal development and postnatal growth, remodeling, and maintenance of a variety of tissues and organs. In accordance with their role in regulating vital biological processes, the amino-acid sequences of homologous TGF-β proteins from d ...
Nutritional Impact on Protein Metabolism of Muscle and
... from the Greek work Proteios (meaning of the first rank). Proteins include several important cell constituents such as enzymes, peptide hormones, antibodies, transport molecules and components of cell skeleton including cell wall. Proteins are the most characteristic chemical compounds found in the ...
... from the Greek work Proteios (meaning of the first rank). Proteins include several important cell constituents such as enzymes, peptide hormones, antibodies, transport molecules and components of cell skeleton including cell wall. Proteins are the most characteristic chemical compounds found in the ...
Journal of Agricultural and Food Chemistry
... and globulin fractions from Amaranthus hypochondriacus and soybean (Glycine max) by electrophoresis, amino acid analysis, fluorescence, hydrophobicity, circular dichroism, and differential scanning calorimetry. The identity and differences between pseudocereal and legume were based on amino acid ana ...
... and globulin fractions from Amaranthus hypochondriacus and soybean (Glycine max) by electrophoresis, amino acid analysis, fluorescence, hydrophobicity, circular dichroism, and differential scanning calorimetry. The identity and differences between pseudocereal and legume were based on amino acid ana ...
Enzymatic Protein Deglycosylation Kit (EDEGLY)
... cleavage is generally the rate limiting reaction, due to the slow removal of some sterically hindered N-linked residues, even when the glycoprotein is denatured. Since all of the enzymes retain activity under reaction conditions for several days, a much larger quantity of glycoprotein may be deglyco ...
... cleavage is generally the rate limiting reaction, due to the slow removal of some sterically hindered N-linked residues, even when the glycoprotein is denatured. Since all of the enzymes retain activity under reaction conditions for several days, a much larger quantity of glycoprotein may be deglyco ...
Insect Biochemistry 15:
... enzyme. After 20 min incubation the reaction was stopped with 1.0ml of a solution containing 0.03 M FeC%, 0.5 M HCI and 0.12 M trichloracetic acid. Reaction mixtures were centrifuged to remove precipitated protein and their absorbance at 540 nm determined. ),-Glutamyl hydroxamate was determined by c ...
... enzyme. After 20 min incubation the reaction was stopped with 1.0ml of a solution containing 0.03 M FeC%, 0.5 M HCI and 0.12 M trichloracetic acid. Reaction mixtures were centrifuged to remove precipitated protein and their absorbance at 540 nm determined. ),-Glutamyl hydroxamate was determined by c ...
protein
... estimated by other means to determine its contribution to the total amino acid composition. ...
... estimated by other means to determine its contribution to the total amino acid composition. ...
Identification of cellular proteins that bind to the human
... al., 1992a) using the pGEX system (Smith & Johnson, 1988). By exploiting the high affinity of GST for glutathione immobilized on agarose beads to generate nef affinity reagents, these fusion proteins appeared to provide the ideal method for identifying nef-binding proteins. However, although GST-nef ...
... al., 1992a) using the pGEX system (Smith & Johnson, 1988). By exploiting the high affinity of GST for glutathione immobilized on agarose beads to generate nef affinity reagents, these fusion proteins appeared to provide the ideal method for identifying nef-binding proteins. However, although GST-nef ...
COMBINATION COMPRISING AT LEAST ONE AMINO ACID AND A
... [0010] In the second step, the binding of mRNA to the 43s preinitiation complex requires a group of proteins collectively referred to as elF4F, a multisubunit complex consisting of elF4A (an RNA helicase), elF4B (which functions in conjunction with elF4A to unwind secondary structure in the 5’ untra ...
... [0010] In the second step, the binding of mRNA to the 43s preinitiation complex requires a group of proteins collectively referred to as elF4F, a multisubunit complex consisting of elF4A (an RNA helicase), elF4B (which functions in conjunction with elF4A to unwind secondary structure in the 5’ untra ...
keratin - trefzclasses
... What is the need for Keratin? • The protein creates a hard surface • Used in hooves, hair, nails • Serve as an insulating layer to protect the delicate new keratin below dead cells because the protein is mostly formed by dead cells which body sheds as new cells pushes up underneath it ...
... What is the need for Keratin? • The protein creates a hard surface • Used in hooves, hair, nails • Serve as an insulating layer to protect the delicate new keratin below dead cells because the protein is mostly formed by dead cells which body sheds as new cells pushes up underneath it ...
Research Applications Of Proteolytic Enzymes In Molecular Biology
... concentrations) can diffuse from the organic phase into the water until the equilibrium is reached; the enzyme-catalyzed synthesis is followed by the diffusion of the products back into the organic phase. The organic phase reduces the dielectric constant of the medium and thus the acidity of the car ...
... concentrations) can diffuse from the organic phase into the water until the equilibrium is reached; the enzyme-catalyzed synthesis is followed by the diffusion of the products back into the organic phase. The organic phase reduces the dielectric constant of the medium and thus the acidity of the car ...
Minimal domain of bacterial phytochrome required for chromophore binding and fluorescence
... Fluorescent proteins (FP) are used to study various biological processes. Recently, a series of nearinfrared (NIR) FPs based on bacterial phytochromes was developed. Finding ways to improve NIR FPs is becoming progressively important. By applying rational design and molecular evolution we have engin ...
... Fluorescent proteins (FP) are used to study various biological processes. Recently, a series of nearinfrared (NIR) FPs based on bacterial phytochromes was developed. Finding ways to improve NIR FPs is becoming progressively important. By applying rational design and molecular evolution we have engin ...
Di-(2-ethylhexyl) phthalate mediates glycolysis and the TCA cycle
... sonicated for 3 min with 5 s of ultrasound and 5 s resting intervals. After centrifugation at 12,000 g for 60 min at 4 °C, the supernatant was collected for desalt treatment. The protein samples were purified using a 2-D Clean-up kit (GE Healthcare, USA) according to the manufacturer’s instructions. ...
... sonicated for 3 min with 5 s of ultrasound and 5 s resting intervals. After centrifugation at 12,000 g for 60 min at 4 °C, the supernatant was collected for desalt treatment. The protein samples were purified using a 2-D Clean-up kit (GE Healthcare, USA) according to the manufacturer’s instructions. ...
Solid Tumour Section Ovary: inv(10)(q11q11) in ovarian germ cell tumors
... Ovary: inv(10)(q11q11) in ovarian germ cell tumors Douglas S Richardson, Lois M Mulligan Department of Pathology and Molecular Medicine, Division of Cancer Biology and Genetics, Cancer ...
... Ovary: inv(10)(q11q11) in ovarian germ cell tumors Douglas S Richardson, Lois M Mulligan Department of Pathology and Molecular Medicine, Division of Cancer Biology and Genetics, Cancer ...
TMEM65 is a mitochondrial inner-membrane protein
... the isolation buffer A [3 mM Hepes-KOH (pH 7.4), 0.21 M mannitol, 0.07 M sucrose, 0.2 mM EGTA] and homogenized with a Dounce homogenizer (Wheaton, Illinois, USA) on ice. The homogenate was overlaid on 0.34 M sucrose and was then centrifuged at 500 × g. The supernatant containing mitochondria and mic ...
... the isolation buffer A [3 mM Hepes-KOH (pH 7.4), 0.21 M mannitol, 0.07 M sucrose, 0.2 mM EGTA] and homogenized with a Dounce homogenizer (Wheaton, Illinois, USA) on ice. The homogenate was overlaid on 0.34 M sucrose and was then centrifuged at 500 × g. The supernatant containing mitochondria and mic ...
Protein purification
Protein purification is a series of processes intended to isolate one or a few proteins from a complex mixture, usually cells, tissues or whole organisms. Protein purification is vital for the characterization of the function, structure and interactions of the protein of interest. The purification process may separate the protein and non-protein parts of the mixture, and finally separate the desired protein from all other proteins. Separation of one protein from all others is typically the most laborious aspect of protein purification. Separation steps usually exploit differences in protein size, physico-chemical properties, binding affinity and biological activity. The pure result may be termed protein isolate.The methods used in protein purification can roughly be divided into analytical and preparative methods. The distinction is not exact, but the deciding factor is the amount of protein that can practically be purified with that method. Analytical methods aim to detect and identify a protein in a mixture, whereas preparative methods aim to produce large quantities of the protein for other purposes, such as structural biology or industrial use. In general, the preparative methods can be used in analytical applications, but not the other way around.