Factors Affecting Adsorption and Pre
... MES at pH 6.1, and the bound protein was determined by BCA assay. IgG was adsorbed to the three particles at 1 mg/mL added protein and varying concentrations of MES from a 500 mM stock at pH 6.1 (Figure 8). The particles were washed, resuspended and assayed as previously described for HSA. ...
... MES at pH 6.1, and the bound protein was determined by BCA assay. IgG was adsorbed to the three particles at 1 mg/mL added protein and varying concentrations of MES from a 500 mM stock at pH 6.1 (Figure 8). The particles were washed, resuspended and assayed as previously described for HSA. ...
Characterization of Proteins Structurally Related to Human N
... association of each isoenzyme with the respective fraction was confirmed by Cellogel electrophoresis as described by Braidman et al. (1974). The recovery of protein was 75-77% of that applied (range from three experiments). Under these conditions the serologically unrelated hexosaminidase C is not r ...
... association of each isoenzyme with the respective fraction was confirmed by Cellogel electrophoresis as described by Braidman et al. (1974). The recovery of protein was 75-77% of that applied (range from three experiments). Under these conditions the serologically unrelated hexosaminidase C is not r ...
Reduced amino acid alphabets exhibit an improved sensitivity and
... of structures and their associated sequences now available in the Protein Data Bank (PDB), it is clear that the same protein fold may be generated by different amino acid sequences; in effect, the structure of proteins is more conserved than their sequences (Chothia and Lesk, 1986). In some cases, t ...
... of structures and their associated sequences now available in the Protein Data Bank (PDB), it is clear that the same protein fold may be generated by different amino acid sequences; in effect, the structure of proteins is more conserved than their sequences (Chothia and Lesk, 1986). In some cases, t ...
A Bayesian network model for protein fold and remote homologue
... were shown to be three times more effective than pairwise methods at detecting remote protein homologies. Further improvement in the sensitivity of remote homologue classification has been obtained by Jaakkola et al. (1999) who have used sequence-based HMMs to develop a discriminative model, based o ...
... were shown to be three times more effective than pairwise methods at detecting remote protein homologies. Further improvement in the sensitivity of remote homologue classification has been obtained by Jaakkola et al. (1999) who have used sequence-based HMMs to develop a discriminative model, based o ...
EXAM 1 learning objectives
... Be able to recognize functional groups Describe the importance of stereospecificity & the use of stereoisomers in biochemistry Be able to pick out a chiral center Describe the five types of biochemical reactions Describe the characteristics of water Describe how important hydrogen bonding is in biom ...
... Be able to recognize functional groups Describe the importance of stereospecificity & the use of stereoisomers in biochemistry Be able to pick out a chiral center Describe the five types of biochemical reactions Describe the characteristics of water Describe how important hydrogen bonding is in biom ...
Sporopollenin biosynthetic enzymes interact and constitute a
... one fluorescent protein, the donor, is transferred to another fluorescent protein, the acceptor. Since efficient energy transfer can only occur if distance between fluorophores is less than 10 nm, FRET can be used to monitor protein-protein interactions in living cells (Sun et al., 2012). To that ai ...
... one fluorescent protein, the donor, is transferred to another fluorescent protein, the acceptor. Since efficient energy transfer can only occur if distance between fluorophores is less than 10 nm, FRET can be used to monitor protein-protein interactions in living cells (Sun et al., 2012). To that ai ...
Identification of a novel viral protein in infectious bursal disease
... purified IPNV. However, no correlation of these products with ORF A-2 has so far been shown, although it has been suggested that this protein is probably virusspecific. Comparison between the amino acid sequences of ORF A-2 proteins of different strains of IBDV and IPNV has indicated only limited am ...
... purified IPNV. However, no correlation of these products with ORF A-2 has so far been shown, although it has been suggested that this protein is probably virusspecific. Comparison between the amino acid sequences of ORF A-2 proteins of different strains of IBDV and IPNV has indicated only limited am ...
Multivalent cross-linking of actin filaments and microtubules through
... Microtubule-associated proteins regulate microtubule dynamics, bundle actin filaments and cross-link actin filaments with microtubules. In addition, aberrant interaction of the microtubule-associated protein Tau with filamentous actin is connected to synaptic impairment in Alzheimer’s disease. Here ...
... Microtubule-associated proteins regulate microtubule dynamics, bundle actin filaments and cross-link actin filaments with microtubules. In addition, aberrant interaction of the microtubule-associated protein Tau with filamentous actin is connected to synaptic impairment in Alzheimer’s disease. Here ...
DOCX - The Human Proteome Project
... extraordinary detections. Time-of-flight, FT-ICR, and Orbitrap-type instruments are considered as having high mass accuracy (when properly calibrated) in these guidelines. Consider alternate explanations of PSMs that appear to indicate extraordinary results. The spectra should be examined closely to ...
... extraordinary detections. Time-of-flight, FT-ICR, and Orbitrap-type instruments are considered as having high mass accuracy (when properly calibrated) in these guidelines. Consider alternate explanations of PSMs that appear to indicate extraordinary results. The spectra should be examined closely to ...
Box 1 - Open Biology
... Mutations in human STIL were first identified as a cause of MCPH in 2009 (1). STIL is located at chromosomal position 1p32.3, has a 5,019bp coding sequence and contains 18 exons. It encodes a 150KDa cytosolic protein that locates to the centrosome during mitosis. Orthologues have been identified and ...
... Mutations in human STIL were first identified as a cause of MCPH in 2009 (1). STIL is located at chromosomal position 1p32.3, has a 5,019bp coding sequence and contains 18 exons. It encodes a 150KDa cytosolic protein that locates to the centrosome during mitosis. Orthologues have been identified and ...
Solutions
... Clinical significance of blood buffers The pH of blood is maintained in a narrow range around 7.4 (7.37 7.43) by the buffer systems in blood plasma and red blood cells. A decrease in blood pH, which can be compensated, is called acidosis while uncompensated decrease in blood pH is called acidaemia. ...
... Clinical significance of blood buffers The pH of blood is maintained in a narrow range around 7.4 (7.37 7.43) by the buffer systems in blood plasma and red blood cells. A decrease in blood pH, which can be compensated, is called acidosis while uncompensated decrease in blood pH is called acidaemia. ...
Photopolymerized Cross-Linked Polyacrylamide Gels for On
... A new method for on-chip sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) of proteins is reported. Miniaturization of SDS-PAGE has attracted significant attention because it offers rapid analysis times, excellent resolution, high throughput, and the potential for integration and ...
... A new method for on-chip sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) of proteins is reported. Miniaturization of SDS-PAGE has attracted significant attention because it offers rapid analysis times, excellent resolution, high throughput, and the potential for integration and ...
Aphelenchoides besseyi
... DH5a for sequence confirmation. Recombinant plasmid DNA was introduced into E. coli BL21(DE3)for expression. Expression of the recombinant protein was examined by sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) and Coomassie brilliant blue staining after treatment with 1 mM isop ...
... DH5a for sequence confirmation. Recombinant plasmid DNA was introduced into E. coli BL21(DE3)for expression. Expression of the recombinant protein was examined by sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) and Coomassie brilliant blue staining after treatment with 1 mM isop ...
Metabolism of “surplus” amino acids
... (78 amino acid) protein that targets tissue proteins for uptake by the proteasome; it is not itself catabolized in the proteasome, but is recycled. However, there is apparently random mono-ubiquitination of proteins in the cell, followed by more specific attachment of three or four additional molecu ...
... (78 amino acid) protein that targets tissue proteins for uptake by the proteasome; it is not itself catabolized in the proteasome, but is recycled. However, there is apparently random mono-ubiquitination of proteins in the cell, followed by more specific attachment of three or four additional molecu ...
Diseases of the Immune System lec.4
... Amyloidosis results from abnormal folding of proteins, which are deposited as fibrils in extracellular tissues and disrupt normal function. Normally, misfolded proteins are degraded intracellularly in proteasomes, or extracellularly by macrophages. It appears that in amyloidosis, these quality contr ...
... Amyloidosis results from abnormal folding of proteins, which are deposited as fibrils in extracellular tissues and disrupt normal function. Normally, misfolded proteins are degraded intracellularly in proteasomes, or extracellularly by macrophages. It appears that in amyloidosis, these quality contr ...
Takanori Nakano
... Finally, I started thinking about burning fat. The fat is fuel for making ATP to keep our body moving. Compared with glucose, scientists say that fat is a more efficient store of energy. In fact, one molecule of glucose can make 36 ATP from both Krebs cycle and Glycolysis, but one fat can make appr ...
... Finally, I started thinking about burning fat. The fat is fuel for making ATP to keep our body moving. Compared with glucose, scientists say that fat is a more efficient store of energy. In fact, one molecule of glucose can make 36 ATP from both Krebs cycle and Glycolysis, but one fat can make appr ...
Extended Detail on Checklist items
... protein or a coding element proposed translation product, justify that other peptides cannot be expected. Single-peptide detections simply have too high a chance of being some type of pernicious false positive to be sufficient for claiming an extraordinary result. Likewise, short peptides of length ...
... protein or a coding element proposed translation product, justify that other peptides cannot be expected. Single-peptide detections simply have too high a chance of being some type of pernicious false positive to be sufficient for claiming an extraordinary result. Likewise, short peptides of length ...
Slide 1
... AS it is proved in past that machine learning techniques are elegant in classifying the biological data. Hau and sun applied the SVM for classification of prokaryotic and eukaryotic proteins and shown that it is better than statistical methods as well as other machine learning techniques such as AN ...
... AS it is proved in past that machine learning techniques are elegant in classifying the biological data. Hau and sun applied the SVM for classification of prokaryotic and eukaryotic proteins and shown that it is better than statistical methods as well as other machine learning techniques such as AN ...
MIAPE_Quant_v1.0_SILAC_MLHS
... calculate a particular protein ratio. They are similar to a coefficient of variation.) Heavy/Light Variability <25 and Heavy/Light Count(the number of peptide ratios that were used to calculate a particular protein ratio) >2 ...
... calculate a particular protein ratio. They are similar to a coefficient of variation.) Heavy/Light Variability <25 and Heavy/Light Count(the number of peptide ratios that were used to calculate a particular protein ratio) >2 ...
Fruitful Collaboration of Physician/Physiologist and Engineer in the
... Our initial collaboration at the University of California Renal Center at San Francisco General Hospital involved determining transport characteristics and the suitability of the new hollow-fiber dialyzers for long-term chronic dialysis. During the initial phases of that program, we encountered epi ...
... Our initial collaboration at the University of California Renal Center at San Francisco General Hospital involved determining transport characteristics and the suitability of the new hollow-fiber dialyzers for long-term chronic dialysis. During the initial phases of that program, we encountered epi ...
Crystallization and X-Ray Crystallographic Studies of Wild
... established by sparse-matrix sampling. The wild-type αTS crystallized as clusters of plate-shaped crystals using a precipitant containing 0.5 M ammonium sulfate, 0.1 M trisodium citrate dihydrate, and 1.0 M lithium sulfate monohydrate (pH 5.6). Under these conditions, crystals appeared after 7−10 da ...
... established by sparse-matrix sampling. The wild-type αTS crystallized as clusters of plate-shaped crystals using a precipitant containing 0.5 M ammonium sulfate, 0.1 M trisodium citrate dihydrate, and 1.0 M lithium sulfate monohydrate (pH 5.6). Under these conditions, crystals appeared after 7−10 da ...
EF-G-GTP
... Pi exchange, or • promote GTP G protein-GDP (inactive) hydrolysis. A guanine nucleotide exchange factor (GEF) induces a conformational change that makes the nucleotide-binding site of a GTP-binding protein more accessible to the aqueous intracellular milieu, where [GTP] [GDP]. Thus a GEF causes a ...
... Pi exchange, or • promote GTP G protein-GDP (inactive) hydrolysis. A guanine nucleotide exchange factor (GEF) induces a conformational change that makes the nucleotide-binding site of a GTP-binding protein more accessible to the aqueous intracellular milieu, where [GTP] [GDP]. Thus a GEF causes a ...
The GPI Transamidase Complex of Saccharomyces
... The preparative SDS-PAGE gel (Figure 2) was stained with silver nitrate, and the interesting bands were cut out and cut into small pieces with a scalpel. Then, 100 l of 25 mM NH4HCO3 were added, gel pieces were agitated with a Vortex for 8 min, and the supernatant was discarded. Washing by agitatio ...
... The preparative SDS-PAGE gel (Figure 2) was stained with silver nitrate, and the interesting bands were cut out and cut into small pieces with a scalpel. Then, 100 l of 25 mM NH4HCO3 were added, gel pieces were agitated with a Vortex for 8 min, and the supernatant was discarded. Washing by agitatio ...
bayesian-integration
... probability of the data given the model (which fraction of ciliary proteins interact with other ciliary proteins) * the prior probability that a protein is ciliary, divided by the sum of all possible probabilities that models gave rise to the data and the probabilities of those models. ...
... probability of the data given the model (which fraction of ciliary proteins interact with other ciliary proteins) * the prior probability that a protein is ciliary, divided by the sum of all possible probabilities that models gave rise to the data and the probabilities of those models. ...
Protein purification
Protein purification is a series of processes intended to isolate one or a few proteins from a complex mixture, usually cells, tissues or whole organisms. Protein purification is vital for the characterization of the function, structure and interactions of the protein of interest. The purification process may separate the protein and non-protein parts of the mixture, and finally separate the desired protein from all other proteins. Separation of one protein from all others is typically the most laborious aspect of protein purification. Separation steps usually exploit differences in protein size, physico-chemical properties, binding affinity and biological activity. The pure result may be termed protein isolate.The methods used in protein purification can roughly be divided into analytical and preparative methods. The distinction is not exact, but the deciding factor is the amount of protein that can practically be purified with that method. Analytical methods aim to detect and identify a protein in a mixture, whereas preparative methods aim to produce large quantities of the protein for other purposes, such as structural biology or industrial use. In general, the preparative methods can be used in analytical applications, but not the other way around.