Post-Transcriptional Regulation of Gene Expression: the Many Hats
... The multi-tasking hnRNP A/B family of proteins play important roles in cytoplasmic mRNA trafficking. Two prime needs for advancement of our understanding of RNA trafficking are the precise identification of further cis-acting elements and their trans-acting factors, and elucidation of the 3D structu ...
... The multi-tasking hnRNP A/B family of proteins play important roles in cytoplasmic mRNA trafficking. Two prime needs for advancement of our understanding of RNA trafficking are the precise identification of further cis-acting elements and their trans-acting factors, and elucidation of the 3D structu ...
MICROSTRUCTURE OF PROTEIN BODIES IN MARAMA BEAN SPECIES
... protein bodies of marama bean. Globoids constitute storage sites for seed phosphorus deposited as insoluble phytate in protein bodies (Martinez, 1979). Spherical globoids are the most common inclusion reported in protein bodies of legumes like peanuts (Young et al., 2004), walnuts and hazelnuts (Lo ...
... protein bodies of marama bean. Globoids constitute storage sites for seed phosphorus deposited as insoluble phytate in protein bodies (Martinez, 1979). Spherical globoids are the most common inclusion reported in protein bodies of legumes like peanuts (Young et al., 2004), walnuts and hazelnuts (Lo ...
Pattern searches for the identification of putative lipoprotein genes in
... autoradiography following protein electrophoresis). This criterion excluded evidence of lipid radiolabelling of the protein following cloning into a heterologous Gram-negative bacterial host (usually E. coli) where the specificities of the Lpp processing enzymes may be subtly different. One exceptio ...
... autoradiography following protein electrophoresis). This criterion excluded evidence of lipid radiolabelling of the protein following cloning into a heterologous Gram-negative bacterial host (usually E. coli) where the specificities of the Lpp processing enzymes may be subtly different. One exceptio ...
Eggs! - Issaquah Connect
... for at least another 2-3 weeks if it has been refrigerated from the time packed until used at 45 degrees F. or lower. As the egg ages, it does lose some of its qualities, so if you were baking a cake or whipping meringue, it might not rise as high as expected, or produce the volume you would expect. ...
... for at least another 2-3 weeks if it has been refrigerated from the time packed until used at 45 degrees F. or lower. As the egg ages, it does lose some of its qualities, so if you were baking a cake or whipping meringue, it might not rise as high as expected, or produce the volume you would expect. ...
Influence of Valine Analogues on Protein Synthesis of Chicken
... 45 min. After centrifugation (5,000×g, 3 min, 4℃), the supernatant was discarded and the pellet was mixed with 15 ml of Medium 199 including 2.5 μg/ml Fungizone, 100 units penicillin, 100 μg/ml streptomycin, 50 μg/ml gentamycin and 10% FCS. Minced muscles were pipetted several times and filtrated by ...
... 45 min. After centrifugation (5,000×g, 3 min, 4℃), the supernatant was discarded and the pellet was mixed with 15 ml of Medium 199 including 2.5 μg/ml Fungizone, 100 units penicillin, 100 μg/ml streptomycin, 50 μg/ml gentamycin and 10% FCS. Minced muscles were pipetted several times and filtrated by ...
Tetracyclines Mechanism of Action
... irritation of the gastric mucosa and is often responsible for noncompliance with tetracyclines. Esophagitis may be minimized through co-administration with food (other than dairy products) or fluids and the use of capsules rather than tablets. 2. Effects on calcified tissues: Deposition in the bone ...
... irritation of the gastric mucosa and is often responsible for noncompliance with tetracyclines. Esophagitis may be minimized through co-administration with food (other than dairy products) or fluids and the use of capsules rather than tablets. 2. Effects on calcified tissues: Deposition in the bone ...
Dynamic properties of a reconstituted myelin sheath
... lipid phase. Indeed, both DOPC and DOPS show a lipid Lα → Lβ phase transition well below room temperature (DOPC: Tα→β = 253 K; DOPS: Tα→β = 264 K). Thus, the mixed lipid compound is in the liquid phase in the entire T range investigated here. While DMPA was chosen to compare results in PNS to our pr ...
... lipid phase. Indeed, both DOPC and DOPS show a lipid Lα → Lβ phase transition well below room temperature (DOPC: Tα→β = 253 K; DOPS: Tα→β = 264 K). Thus, the mixed lipid compound is in the liquid phase in the entire T range investigated here. While DMPA was chosen to compare results in PNS to our pr ...
Gene Section BRD4 (bromodomain containing 4) Atlas of Genetics and Cytogenetics
... The BRD4-NUT fusion protein is composed of the Nterminal of BRD4 (amino acids 1-720 out of 1372) and almost the entire protein sequence of NUT (amino acids 6-1127). The N-terminal of BRD4 includes bromodomains 1 and 2 and other, less well ...
... The BRD4-NUT fusion protein is composed of the Nterminal of BRD4 (amino acids 1-720 out of 1372) and almost the entire protein sequence of NUT (amino acids 6-1127). The N-terminal of BRD4 includes bromodomains 1 and 2 and other, less well ...
Protein kinases - Institut de recherches cliniques de Montréal
... How many phosphorylation sites are there? If there are ~10,000 proteins per cell with an average length of 400 aa (~ 17% of which are Ser, Thr or Tyr), then there are ~700,000 potential phosphorylation sites for any given kinase (including hidden residues). Although protein kinases have relatively ...
... How many phosphorylation sites are there? If there are ~10,000 proteins per cell with an average length of 400 aa (~ 17% of which are Ser, Thr or Tyr), then there are ~700,000 potential phosphorylation sites for any given kinase (including hidden residues). Although protein kinases have relatively ...
Protein 4.2 interaction with hereditary spherocytosis mutants of the
... predominant of two protein 4.2 splice variants. Type I is the less common larger isoform and has an additional 30 amino acids near the N-terminus [11]. Amino acid numbering of protein 4.2 in the present paper will be according to the Type II isoform. Protein 4.2 is a member of the TG (transglutamina ...
... predominant of two protein 4.2 splice variants. Type I is the less common larger isoform and has an additional 30 amino acids near the N-terminus [11]. Amino acid numbering of protein 4.2 in the present paper will be according to the Type II isoform. Protein 4.2 is a member of the TG (transglutamina ...
Nutrition for Endurance Athletes
... supplements to replenish the vitamins and minerals leached from the body as a result of perspiring. Our products contain these vitamins and minerals and ensure that athletes get the right amount. CARBOHYDRATES Not only is the amount of carbs crucial but also their composition. Some carbs, such as gl ...
... supplements to replenish the vitamins and minerals leached from the body as a result of perspiring. Our products contain these vitamins and minerals and ensure that athletes get the right amount. CARBOHYDRATES Not only is the amount of carbs crucial but also their composition. Some carbs, such as gl ...
Protein Nutrition of Dairy Cattle – An Overview
... amino acid composition of ruminant products. In contrast, estimates of amino acid requirements for maintenance have remained elusive. Our comprehension of amino acid (and protein) metabolism and requirements for animals and humans is based on knowledge gained from thousands of studies conducted with ...
... amino acid composition of ruminant products. In contrast, estimates of amino acid requirements for maintenance have remained elusive. Our comprehension of amino acid (and protein) metabolism and requirements for animals and humans is based on knowledge gained from thousands of studies conducted with ...
Serine phosphorylation of the cotton cytosolic pyruvate kinase
... Phosphorylation of GhPK6 regulated its stability The effect of GhPK6 phosphorylation on its stability was also investigated. The half-life of the GhPK6 protein is approximately 60 min. The mutation of serine 215 to alanine increased its half-life to more than 120 min, whereas mutation of the same re ...
... Phosphorylation of GhPK6 regulated its stability The effect of GhPK6 phosphorylation on its stability was also investigated. The half-life of the GhPK6 protein is approximately 60 min. The mutation of serine 215 to alanine increased its half-life to more than 120 min, whereas mutation of the same re ...
Slide 1
... •Select FER1_SPIOL. Right click on FER1_SPIOL. Select structure > Associate structure with sequences > discover PDB ids. •Now again, right click on FER1_SPIOL. Select structure > View structure of FER1_SPIOL. This will open a window where you can view its structure (PDB 1A70). The viewer is Jmol. Tr ...
... •Select FER1_SPIOL. Right click on FER1_SPIOL. Select structure > Associate structure with sequences > discover PDB ids. •Now again, right click on FER1_SPIOL. Select structure > View structure of FER1_SPIOL. This will open a window where you can view its structure (PDB 1A70). The viewer is Jmol. Tr ...
AMIN domains have a predicted role in localization of diverse
... AMIN domain with domains that form unrelated, but functionally comparable, transport structures across the Gram-negative outer membranes. These include the secretin module in PilQ, which is involved in transport of type IV pilus components across the outer membrane, the TonB-dependent receptors, whi ...
... AMIN domain with domains that form unrelated, but functionally comparable, transport structures across the Gram-negative outer membranes. These include the secretin module in PilQ, which is involved in transport of type IV pilus components across the outer membrane, the TonB-dependent receptors, whi ...
SUPPLEMENTARY MATERIAL Title: Luteolin attenuate the D
... Japan). All other reagents used in the experiments were of analytical grade and purity. 1.2 Animal and tissue preparation Adult male Kunming mice (6 weeks old, weighing 18-23g) were randomized into 4 groups (n = 12 each): Group 1 (control group): mice received daily intraperitoneally injected of sal ...
... Japan). All other reagents used in the experiments were of analytical grade and purity. 1.2 Animal and tissue preparation Adult male Kunming mice (6 weeks old, weighing 18-23g) were randomized into 4 groups (n = 12 each): Group 1 (control group): mice received daily intraperitoneally injected of sal ...
Response of Jujube Fruits to Exogenous Oxalic Acid Treatment
... treatment. In order to determine the details of protein spots in Fig. 5, we magnified the areas of protein spots of interest in Fig. 7A, in which the marked numbers of the protein spots are the same as those in Fig. 5. Fig. 7B shows the abundance of the proteins which are involved in metabolism and e ...
... treatment. In order to determine the details of protein spots in Fig. 5, we magnified the areas of protein spots of interest in Fig. 7A, in which the marked numbers of the protein spots are the same as those in Fig. 5. Fig. 7B shows the abundance of the proteins which are involved in metabolism and e ...
Can correct protein models be identified?
... Born solvation model (Still et al. 1990) as a description of solvent effects, physical energy functions can be used to identify the native conformation (Dominy and Brooks 2002; Felts et al. 2002). Knowledge-based energy functions are calculated from the difference between features of a random protei ...
... Born solvation model (Still et al. 1990) as a description of solvent effects, physical energy functions can be used to identify the native conformation (Dominy and Brooks 2002; Felts et al. 2002). Knowledge-based energy functions are calculated from the difference between features of a random protei ...
The cyanide hydratase from Neurospora crassa forms a helix
... 3 h, following which the cells were pelleted by centrifugation. The pellet was resuspended in 50 mM Tris/Cl buffer containing 100 mM NaCl pH 8.0 and sonicated on ice for a total period of 4 min. The lysate was then clarified of cell debris by centrifugation. Protein was precipitated with ammonium su ...
... 3 h, following which the cells were pelleted by centrifugation. The pellet was resuspended in 50 mM Tris/Cl buffer containing 100 mM NaCl pH 8.0 and sonicated on ice for a total period of 4 min. The lysate was then clarified of cell debris by centrifugation. Protein was precipitated with ammonium su ...
Introduction to Database Searching using MASCOT
... One of the strengths of PMF is that it is an easy experiment that can be performed using just about any mass spectrometer. The whole process is readily automated and MALDI instruments, in particular, can churn out high accuracy PMF data at a very high rate. In principal, it is a sensitive technique ...
... One of the strengths of PMF is that it is an easy experiment that can be performed using just about any mass spectrometer. The whole process is readily automated and MALDI instruments, in particular, can churn out high accuracy PMF data at a very high rate. In principal, it is a sensitive technique ...
TARGET: a new method for predicting protein subcellular
... AAC scores have been calculated separately for each of the nine locations where the location with highest score wins the prediction. For each current location, there are 16 ‘other’ locations including 8 NT and 8 CT locations, and the AAC score for the current location is the sum of 16 arbitrary scor ...
... AAC scores have been calculated separately for each of the nine locations where the location with highest score wins the prediction. For each current location, there are 16 ‘other’ locations including 8 NT and 8 CT locations, and the AAC score for the current location is the sum of 16 arbitrary scor ...
Protein profiling of the dimorphic, pathogenic fungus,Penicillium
... filamentously (mould phase), but at body temperature (37°C), a uninucleate yeast form develops that reproduces by fission. Formation of the yeast phase appears to be a requisite for pathogenicity. To date, no genes have been identified in P. marneffei that strictly induce mould-to-yeast phase conver ...
... filamentously (mould phase), but at body temperature (37°C), a uninucleate yeast form develops that reproduces by fission. Formation of the yeast phase appears to be a requisite for pathogenicity. To date, no genes have been identified in P. marneffei that strictly induce mould-to-yeast phase conver ...
Dirty Business - American Chemical Society
... largest known protein, consists of 34,350 amino acids, with a molecular mass of approximately 3.8 million Daltons. Protein molecules are so large they are often referred to as macromolecules. In addition to the large range in molecular size, proteins have a wide variety of properties. For example, s ...
... largest known protein, consists of 34,350 amino acids, with a molecular mass of approximately 3.8 million Daltons. Protein molecules are so large they are often referred to as macromolecules. In addition to the large range in molecular size, proteins have a wide variety of properties. For example, s ...
Fast procedure for reconstruction of full
... Rotkiewicz and Skolnick • Vol. 00, No. 00 • Journal of Computational Chemistry ...
... Rotkiewicz and Skolnick • Vol. 00, No. 00 • Journal of Computational Chemistry ...
Protein purification
Protein purification is a series of processes intended to isolate one or a few proteins from a complex mixture, usually cells, tissues or whole organisms. Protein purification is vital for the characterization of the function, structure and interactions of the protein of interest. The purification process may separate the protein and non-protein parts of the mixture, and finally separate the desired protein from all other proteins. Separation of one protein from all others is typically the most laborious aspect of protein purification. Separation steps usually exploit differences in protein size, physico-chemical properties, binding affinity and biological activity. The pure result may be termed protein isolate.The methods used in protein purification can roughly be divided into analytical and preparative methods. The distinction is not exact, but the deciding factor is the amount of protein that can practically be purified with that method. Analytical methods aim to detect and identify a protein in a mixture, whereas preparative methods aim to produce large quantities of the protein for other purposes, such as structural biology or industrial use. In general, the preparative methods can be used in analytical applications, but not the other way around.