Supplementary Notes - Word file
... that emit green (PplGR) and yellow-green light (PplYG) possess an Ile residue at the position corresponding to Ile288 of Lcr luciferase, but this position is substituted with a Val residue in the Ppl luciferases that emit light with longer wavelengths: yellow (PplYE) and orange (PplOR) (Fig. S2). We ...
... that emit green (PplGR) and yellow-green light (PplYG) possess an Ile residue at the position corresponding to Ile288 of Lcr luciferase, but this position is substituted with a Val residue in the Ppl luciferases that emit light with longer wavelengths: yellow (PplYE) and orange (PplOR) (Fig. S2). We ...
Applied BioChem by AJM QUALITATIVE ANALYSIS OF AMINO
... react with ninhydrin at pH=4. The reduction product obtained from ninhydrin then reacts with NH 3 and excess ninhydrin to yield a blue colored substance. This reaction provides an extremely sensitive test for amino acids. Apply this test to any of the amino acids you choose. WARNING: Avoid spilling ...
... react with ninhydrin at pH=4. The reduction product obtained from ninhydrin then reacts with NH 3 and excess ninhydrin to yield a blue colored substance. This reaction provides an extremely sensitive test for amino acids. Apply this test to any of the amino acids you choose. WARNING: Avoid spilling ...
Yeast ING Protein Yeast Protein Human Ortholog Description of
... exposure to mutagens, also induced during meiosis at a time nearly coincident with commitment to recombination D-lactate dehydrogenase, part of the retrograde regulon which consists of genes whose expression is stimulated by damage to mitochondria and reduced in cells grown with glutamate as the sol ...
... exposure to mutagens, also induced during meiosis at a time nearly coincident with commitment to recombination D-lactate dehydrogenase, part of the retrograde regulon which consists of genes whose expression is stimulated by damage to mitochondria and reduced in cells grown with glutamate as the sol ...
Phytochemistry 24:
... of S-ADP-Agarose in purification of pea seed GS [25]; possibly, the use of Mg2+ rather than Mn2+ ions in the buffer may explain their result. Dowton and Kennedy (unpublished) have successfully employed 2’,5’-ADPSepharose chromatography to purify an insect GS and this affinity procedure may prove of ...
... of S-ADP-Agarose in purification of pea seed GS [25]; possibly, the use of Mg2+ rather than Mn2+ ions in the buffer may explain their result. Dowton and Kennedy (unpublished) have successfully employed 2’,5’-ADPSepharose chromatography to purify an insect GS and this affinity procedure may prove of ...
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... amino acid sequences and surface charges based on corn genotypes, location of protein bodies in the kernel and age of the kernel. The composition of the zein products, in terms of the relative proportions of zein fractions, could have an effect on the functional properties of zein and its utilizatio ...
... amino acid sequences and surface charges based on corn genotypes, location of protein bodies in the kernel and age of the kernel. The composition of the zein products, in terms of the relative proportions of zein fractions, could have an effect on the functional properties of zein and its utilizatio ...
The integrity of a cholesterol-binding pocket in Niemann–Pick C2
... and Methods. The purity, assessed by staining for total protein, was estimated at ⬇60% after the Ni-affinity step and ⬎90% after gel filtration (Fig. 1B). Complementation of npc2 cells showed that the purified protein was highly active, with an EC50 of 1.5 nM (assuming an average molecular mass of 2 ...
... and Methods. The purity, assessed by staining for total protein, was estimated at ⬇60% after the Ni-affinity step and ⬎90% after gel filtration (Fig. 1B). Complementation of npc2 cells showed that the purified protein was highly active, with an EC50 of 1.5 nM (assuming an average molecular mass of 2 ...
Regulation of Protein Degradation
... and analyses of proteinases. Although studies using heterologous proteins or synthetic peptide substrates will continue to provide valuable information, such studies must at some point utilize in vivo substrates if the physiological roles of proteinases are to be defined. Finally, no particular glob ...
... and analyses of proteinases. Although studies using heterologous proteins or synthetic peptide substrates will continue to provide valuable information, such studies must at some point utilize in vivo substrates if the physiological roles of proteinases are to be defined. Finally, no particular glob ...
Mutational effects on protein structure and function Jonas Carlsson Link¨
... In this thesis several important proteins are investigated from a structural perspective. Some of the proteins are disease related while other have important but not completely characterised functions. The techniques used are general as demonstrated by applications on metabolic proteins (CYP21, CYP1 ...
... In this thesis several important proteins are investigated from a structural perspective. Some of the proteins are disease related while other have important but not completely characterised functions. The techniques used are general as demonstrated by applications on metabolic proteins (CYP21, CYP1 ...
Chromatography
... APPLICATIONS OF PAPER CHROMATOGRAPHY It is used in separation of amino acids , sugar from urine , drugs from the samples of blood and colors from dyes . It is used for the detection of adulterants and contaminants in foods and drinks . It is used for the detection of drugs and dopes in animals ...
... APPLICATIONS OF PAPER CHROMATOGRAPHY It is used in separation of amino acids , sugar from urine , drugs from the samples of blood and colors from dyes . It is used for the detection of adulterants and contaminants in foods and drinks . It is used for the detection of drugs and dopes in animals ...
GuanHongLi (275
... Long rice with protein content of 8.8% was obtained from local market. ACE (from rabbit lung; 3.4units/mg of protein), pepsin (1:60,000, 3400 U/mg of solid, from porcine stomach mucosa) and hippuryl-L-histidyl-L-leucine (HipHis-Leu) were purchased from Sigma. Alcalase 2.4L (liquid, 2.4 AU/g) was kin ...
... Long rice with protein content of 8.8% was obtained from local market. ACE (from rabbit lung; 3.4units/mg of protein), pepsin (1:60,000, 3400 U/mg of solid, from porcine stomach mucosa) and hippuryl-L-histidyl-L-leucine (HipHis-Leu) were purchased from Sigma. Alcalase 2.4L (liquid, 2.4 AU/g) was kin ...
SB401, a pollen-specific protein from Solanum berthaultii
... (f) The MT meshwork separated into small bunches of MT bundles when incubated with 50 mM NaCl. (g) The bunches of MT bundles separated into thinner MT bundles when incubated with 100 mM NaCl. (h) All MTs appeared as single MT filaments after adding 200 mM NaCl. Bar in (h) = 10 lM, and applies to (a– ...
... (f) The MT meshwork separated into small bunches of MT bundles when incubated with 50 mM NaCl. (g) The bunches of MT bundles separated into thinner MT bundles when incubated with 100 mM NaCl. (h) All MTs appeared as single MT filaments after adding 200 mM NaCl. Bar in (h) = 10 lM, and applies to (a– ...
Synthetic human prion protein octapeptide repeat binds to the
... disordered N-terminal tail [7]. The helical domain represents a highly ordered compact structure and probably is less susceptible to proteolysis, at least in the beginning of the hydrolytic attack, than the tail. The N-terminal domain of the prion molecule becomes more structured after binding coppe ...
... disordered N-terminal tail [7]. The helical domain represents a highly ordered compact structure and probably is less susceptible to proteolysis, at least in the beginning of the hydrolytic attack, than the tail. The N-terminal domain of the prion molecule becomes more structured after binding coppe ...
Computer-Based Design of Novel Protein Structures
... amino acid sequences that could pack well on a target protein backbone. These algorithms have been successful and have been used to stabilize proteins, solubilize membrane proteins, redesign protein-protein interactions, create new enzymes, and design novel protein structures (6, 15, 35, 50, 72). In ...
... amino acid sequences that could pack well on a target protein backbone. These algorithms have been successful and have been used to stabilize proteins, solubilize membrane proteins, redesign protein-protein interactions, create new enzymes, and design novel protein structures (6, 15, 35, 50, 72). In ...
Elements of Systemic..
... The primary structure refers to amino acid sequence of the polypeptide chain. The primary structure is held together by covalent or peptide bonds, which are made during the process of protein biosynthesis or translation. The two ends of the polypeptide chain are referred to as the carboxyl terminus ...
... The primary structure refers to amino acid sequence of the polypeptide chain. The primary structure is held together by covalent or peptide bonds, which are made during the process of protein biosynthesis or translation. The two ends of the polypeptide chain are referred to as the carboxyl terminus ...
University of Groningen Sugar transport in
... the periplasm (Gram-negative) or are attached to the membrane via a fatty acid modification of the amino-terminus (Gram-positive) (Fig. 2). In archaea, binding proteins are membrane-bound by means of a hydrophobic transmembrane segment that can be present at either the N-terminus or C-terminus. Sequ ...
... the periplasm (Gram-negative) or are attached to the membrane via a fatty acid modification of the amino-terminus (Gram-positive) (Fig. 2). In archaea, binding proteins are membrane-bound by means of a hydrophobic transmembrane segment that can be present at either the N-terminus or C-terminus. Sequ ...
Dephosphorylation of the Calcium Pump Coupled to Counterion
... positively charged residues (lysines and arginines) are not near enough the glutamate and aspartate residues to interact. This is energetically unfavourable: D and E residues are charged and polar, and prefer a hydrophilic environment. The transmembrane helices, however, are quite hydrophobic. ...
... positively charged residues (lysines and arginines) are not near enough the glutamate and aspartate residues to interact. This is energetically unfavourable: D and E residues are charged and polar, and prefer a hydrophilic environment. The transmembrane helices, however, are quite hydrophobic. ...
Determination of Protein Concentrations Using AAA
... glycol) may hinder access of dyes through steric hindrance. Proteins with an exceptionally low composition of dye-binding amino acids (e.g., collagen) produce lower color development and thus underestimate the protein content when calibrated against a dissimilar protein (e.g., BSA). Under conditions ...
... glycol) may hinder access of dyes through steric hindrance. Proteins with an exceptionally low composition of dye-binding amino acids (e.g., collagen) produce lower color development and thus underestimate the protein content when calibrated against a dissimilar protein (e.g., BSA). Under conditions ...
Antifolding activity of hsp60 couples protein import into the
... interaction between the pb2-DHFR fusion proteins and hsp60-14mer. The topology of mature-sized b2(331)DHFR arrested in contact sites in the presence of MTX was analyzed as a representative example (Figure 4). Upon removal of the MTX-stabilized DHFR moiety by protease treatment, the resulting cytochr ...
... interaction between the pb2-DHFR fusion proteins and hsp60-14mer. The topology of mature-sized b2(331)DHFR arrested in contact sites in the presence of MTX was analyzed as a representative example (Figure 4). Upon removal of the MTX-stabilized DHFR moiety by protease treatment, the resulting cytochr ...
Topology and Phosphorylation of Soybean Nodulin
... PBM proteins was started by the addition of 1 #Ci of ~-32P-ATP (specific activity 3,000 Ci/mmol; DuFOnt/NEN) to 20 ~1 of the PBM suspension and the reaction was allowed to proceed for 20 min at room temperature (Suzuki and Verma, 1989). The phosphorylated PBM was pelleted in a microcentrifuge and wa ...
... PBM proteins was started by the addition of 1 #Ci of ~-32P-ATP (specific activity 3,000 Ci/mmol; DuFOnt/NEN) to 20 ~1 of the PBM suspension and the reaction was allowed to proceed for 20 min at room temperature (Suzuki and Verma, 1989). The phosphorylated PBM was pelleted in a microcentrifuge and wa ...
... New knowledge can only be found by making use of the knowledge that already is there. In the following sections some general issues concerning enzymatic reactions and the regulation of enzymes are touched upon. Enzymes are proteins that catalyze chemical reactions. A chemical reaction can only occur ...
Interacting specificity of a histidine kinase and its cognate response
... sequence upstream of the prrA start codon in addition to the entire prrA gene. Since this additional DNA sequence does not contain a stop codon that lies in the same reading frame as the GAL4AD and prrA genes, the PrrA protein expressed from pPLAC (pGADT7 : : prrA) has an additional 28 amino acid re ...
... sequence upstream of the prrA start codon in addition to the entire prrA gene. Since this additional DNA sequence does not contain a stop codon that lies in the same reading frame as the GAL4AD and prrA genes, the PrrA protein expressed from pPLAC (pGADT7 : : prrA) has an additional 28 amino acid re ...
Multiple linear regression for protein secondary structure prediction
... Prediction of the three-dimensional structure of a protein from its sequence is becoming a pressing problem for many biologists because the discrepancy continues to increase between the number of known protein sequences and the number of experimentally determined structures. The prediction of second ...
... Prediction of the three-dimensional structure of a protein from its sequence is becoming a pressing problem for many biologists because the discrepancy continues to increase between the number of known protein sequences and the number of experimentally determined structures. The prediction of second ...
Adaptative biochemical pathways and regulatory networks in
... To investigate iron-dependent cell processes and regulatory networks thereof associated to citrate fermentation and EPS production, BAS-10 was cultivated: i) under aerobic conditions on Fe(III)citrate (FEC) as sole carbon source; ii) under anaerobic conditions using FEC or iii) Na(I)-citrate (NAC) a ...
... To investigate iron-dependent cell processes and regulatory networks thereof associated to citrate fermentation and EPS production, BAS-10 was cultivated: i) under aerobic conditions on Fe(III)citrate (FEC) as sole carbon source; ii) under anaerobic conditions using FEC or iii) Na(I)-citrate (NAC) a ...
Protein purification
Protein purification is a series of processes intended to isolate one or a few proteins from a complex mixture, usually cells, tissues or whole organisms. Protein purification is vital for the characterization of the function, structure and interactions of the protein of interest. The purification process may separate the protein and non-protein parts of the mixture, and finally separate the desired protein from all other proteins. Separation of one protein from all others is typically the most laborious aspect of protein purification. Separation steps usually exploit differences in protein size, physico-chemical properties, binding affinity and biological activity. The pure result may be termed protein isolate.The methods used in protein purification can roughly be divided into analytical and preparative methods. The distinction is not exact, but the deciding factor is the amount of protein that can practically be purified with that method. Analytical methods aim to detect and identify a protein in a mixture, whereas preparative methods aim to produce large quantities of the protein for other purposes, such as structural biology or industrial use. In general, the preparative methods can be used in analytical applications, but not the other way around.