Methodology for predicting semantic annotations of protein
... a protein sequence is decomposed into a numerical series by a contact potential. From the interactions between adjacent amino acids, the wavelet transform can easily detect and characterize subsequences at specific position along the protein sequence. Then, all subsequences are grouped into clusters ...
... a protein sequence is decomposed into a numerical series by a contact potential. From the interactions between adjacent amino acids, the wavelet transform can easily detect and characterize subsequences at specific position along the protein sequence. Then, all subsequences are grouped into clusters ...
Identification of proteins that putatively bind the
... based on turgor pressure-induced changes in their shape. Stomatal aperture and density are affected by environmental stimuli such as light quality and quantity, CO2 concentrations, and water availability. The basic helix-loop-helix (bHLH) transcription factor MUTE drives the differentiation of trans ...
... based on turgor pressure-induced changes in their shape. Stomatal aperture and density are affected by environmental stimuli such as light quality and quantity, CO2 concentrations, and water availability. The basic helix-loop-helix (bHLH) transcription factor MUTE drives the differentiation of trans ...
Elastin
... the hydrophobic force. It is easily deformed to an extended configuration, but will relax back to a compact conformation. ...
... the hydrophobic force. It is easily deformed to an extended configuration, but will relax back to a compact conformation. ...
a server for analyzing and predicting RNA
... accuracy, using only the amino acid sequence of a query protein (and no information about the bound RNA) as input (7). In the current web-based implementation, RNABindR allows users to: (i) identify actual binding residues for a given protein–RNA complex in the Protein Data Bank (PDB) (8) and (ii) p ...
... accuracy, using only the amino acid sequence of a query protein (and no information about the bound RNA) as input (7). In the current web-based implementation, RNABindR allows users to: (i) identify actual binding residues for a given protein–RNA complex in the Protein Data Bank (PDB) (8) and (ii) p ...
Protein translocation channel of mitochondrial inner
... Mokranjac et al., 2003b). In this way, Tim44 likely ensures that binding of mtHsp70 to the translocating polypeptides, regulated by the action of Tim14 and Tim16, takes place right at the outlet of the translocation channel in the inner membrane. Tim44 is composed of two domains, depicted as N- and ...
... Mokranjac et al., 2003b). In this way, Tim44 likely ensures that binding of mtHsp70 to the translocating polypeptides, regulated by the action of Tim14 and Tim16, takes place right at the outlet of the translocation channel in the inner membrane. Tim44 is composed of two domains, depicted as N- and ...
Basic Peptide Bonds
... This process could be used to treat certain types of cancer, though the process for synthesizing these peptides or applying them in a pharmaceutical application is still being investigated for its potential methodology. Protein tags that can be used to identify the protein structures involved in thi ...
... This process could be used to treat certain types of cancer, though the process for synthesizing these peptides or applying them in a pharmaceutical application is still being investigated for its potential methodology. Protein tags that can be used to identify the protein structures involved in thi ...
Recombinant DNA in Medicine Industry
... •Antibodies are exquisitely selective proteins that can bind to a single target among millions of irrelevant sites •Could effectively seek and destroy tumor cells and infectious agents wherever the reside ...
... •Antibodies are exquisitely selective proteins that can bind to a single target among millions of irrelevant sites •Could effectively seek and destroy tumor cells and infectious agents wherever the reside ...
Types and effects of protein variations. Vihinen
... “Protein truncation” is a special case of deletion where deletion occurs either in N- or Cterminus. Indels originate due to both insertion and deletion. The different types of protein variations in a short protein sequence for alanine, alanine, serine… in one letter code AASEQWENCE are depicted in F ...
... “Protein truncation” is a special case of deletion where deletion occurs either in N- or Cterminus. Indels originate due to both insertion and deletion. The different types of protein variations in a short protein sequence for alanine, alanine, serine… in one letter code AASEQWENCE are depicted in F ...
Why nature chose phosphate to modify proteins
... triphosphates was the key to the formation of polynucleotides, and the encoding of genetic information. The selection of ATP as the major energy storage compound also meant the ready availability of activated phosphate groups for transfer to other molecules. Because of its energy storage function, A ...
... triphosphates was the key to the formation of polynucleotides, and the encoding of genetic information. The selection of ATP as the major energy storage compound also meant the ready availability of activated phosphate groups for transfer to other molecules. Because of its energy storage function, A ...
The protein acetylome and the regulation of metabolism - Serval
... (NatC) contains the Maintenance of Killer proteins Mak3, Mak10 and Mak31. Homologs to all these subunits are present in plants, and complementation of yeast natc mutants with the homologous Arabidopsis (Arabidopsis thaliana) genes has been reported [20]. NatA mainly recognizes N-terminal single amin ...
... (NatC) contains the Maintenance of Killer proteins Mak3, Mak10 and Mak31. Homologs to all these subunits are present in plants, and complementation of yeast natc mutants with the homologous Arabidopsis (Arabidopsis thaliana) genes has been reported [20]. NatA mainly recognizes N-terminal single amin ...
Controlling subcellular delivery to optimize
... Lysosomes contain hydrolytic enzymes for digestion of phagocytosed materials, and macromolecules (derived intra- or extra-cellular), and even for production of nutrients [19] . The classic example uses PEI for endosomal escape [61] . PEI’s secondary and tertiary amines are protonated in the acidic e ...
... Lysosomes contain hydrolytic enzymes for digestion of phagocytosed materials, and macromolecules (derived intra- or extra-cellular), and even for production of nutrients [19] . The classic example uses PEI for endosomal escape [61] . PEI’s secondary and tertiary amines are protonated in the acidic e ...
Free amino acids are important for the retention of protein and non
... It is difficult to compare the experiments of Cole and Gillett with those reported here; we looked at the presence of meal constituents in the midgut and faeces (protein and FITC-dextran), while they looked at elimination of the dark brown haem-containing faeces. These two processes are likely contr ...
... It is difficult to compare the experiments of Cole and Gillett with those reported here; we looked at the presence of meal constituents in the midgut and faeces (protein and FITC-dextran), while they looked at elimination of the dark brown haem-containing faeces. These two processes are likely contr ...
Localization of Protein-Protein lnteractions between Subunits of
... fine mapping of dimerization regions or, more precisely, sites of homotypic protein-protein interaction, and avoids the problems mentioned above. The premise of our assay is to begin with a protein that must be a dimer to function, remove that protein's dimerization region, and replace it with segme ...
... fine mapping of dimerization regions or, more precisely, sites of homotypic protein-protein interaction, and avoids the problems mentioned above. The premise of our assay is to begin with a protein that must be a dimer to function, remove that protein's dimerization region, and replace it with segme ...
Directions: Choose the BEST answer from among those given.
... 21)The type of protein, often composed of multiple subunits, that exists in two or more conformations depending upon the binding of a specific ligand at a site other that the catalytic site is a(n) ? a) acyl-carrier protein b) redox protein c) allosteric protein d) denatured protein e) choose this ...
... 21)The type of protein, often composed of multiple subunits, that exists in two or more conformations depending upon the binding of a specific ligand at a site other that the catalytic site is a(n) ? a) acyl-carrier protein b) redox protein c) allosteric protein d) denatured protein e) choose this ...
Cysteine 230 Modulates Tumor Necrosis Factor
... functional TRAIL-Rs through which the apoptosis-inducing activity of TRAIL is transmitted into the cytoplasm. DcR1 and DcR2 are truncated TRAIL-Rs in which the cytoplasmic regions containing the death domains are deleted. Thus, overexpression of DcR1 and DcR2 blocks the function of DR4 and DR5 (13, ...
... functional TRAIL-Rs through which the apoptosis-inducing activity of TRAIL is transmitted into the cytoplasm. DcR1 and DcR2 are truncated TRAIL-Rs in which the cytoplasmic regions containing the death domains are deleted. Thus, overexpression of DcR1 and DcR2 blocks the function of DR4 and DR5 (13, ...
The role of mutations in core protein of hepatitis B
... related liver diseases. Patients with long standing active liver disease are at high risk to develop liver cirrhosis or hepatocellular carcinoma [3]. The genome of hepatitis B virus encodes four overlapping open reading frames that are translated to viral core protein or HBc particle, the surface pr ...
... related liver diseases. Patients with long standing active liver disease are at high risk to develop liver cirrhosis or hepatocellular carcinoma [3]. The genome of hepatitis B virus encodes four overlapping open reading frames that are translated to viral core protein or HBc particle, the surface pr ...
OMB No. 0925-0001/0002 (Rev. 08/12), Biographical Sketch Format
... clinical trials (using glutaldehyde crosslinked KLH/antibody complex), we were motivated to develop more effective vaccine designs that could still be supplied at patient-specific vaccines. HepBcore VLPs proved to provide the best scaffolding for a viral mimic nanoparticle, modular design. Uniquely ...
... clinical trials (using glutaldehyde crosslinked KLH/antibody complex), we were motivated to develop more effective vaccine designs that could still be supplied at patient-specific vaccines. HepBcore VLPs proved to provide the best scaffolding for a viral mimic nanoparticle, modular design. Uniquely ...
HMB HMB for Muscle Health HMB Promotes an Anabolic State and
... pathway known as ubiquitination. In this pathway a small molecule called ubiquitin (because it is found in all cell types) is linked to protein molecules. This targets them for degradation in proteasomes. Obviously if you are a body builder or an athlete you don’t want your proteins to be degraded. ...
... pathway known as ubiquitination. In this pathway a small molecule called ubiquitin (because it is found in all cell types) is linked to protein molecules. This targets them for degradation in proteasomes. Obviously if you are a body builder or an athlete you don’t want your proteins to be degraded. ...
Does Plant Cell Death Induced by Ptr ToxA Require Toxin Entry?
... Green Fluorescent Protein Sensitive ...
... Green Fluorescent Protein Sensitive ...
The extraction of collagen protein from pigskin
... conditions. From this study, the order of hydrolysis ability was proved to be: pancreatin > papain > pepsin. The triple helix structure of the collagen protein obtained from pepsin hydrolysis was maintained relatively well. Feng Zhang et al. [24] used neutral protease to treat leather materials. Dur ...
... conditions. From this study, the order of hydrolysis ability was proved to be: pancreatin > papain > pepsin. The triple helix structure of the collagen protein obtained from pepsin hydrolysis was maintained relatively well. Feng Zhang et al. [24] used neutral protease to treat leather materials. Dur ...
Application Sheet S13 - AXIS-SHIELD Density Gradient Media
... at a lower g-force. The precise conditions required for peroxisome purification in self-generated gradients, depends very much on the rotor type. Any vertical rotor, with a sedimentation path length of <25 mm would provide a very simple and efficient system; at approx 350,000gav separation would tak ...
... at a lower g-force. The precise conditions required for peroxisome purification in self-generated gradients, depends very much on the rotor type. Any vertical rotor, with a sedimentation path length of <25 mm would provide a very simple and efficient system; at approx 350,000gav separation would tak ...
Unicellular Eukaryotes to Humans Protein Arginine
... arginines were identified 40 years ago from acidic lysates of nuclear extracts (69). However, the first genes encoding protein arginine methyltransferases (PRMTs) were identified only a decade ago (29, 37, 54). Since this important discovery, the study of protein arginine methylation has been a rapi ...
... arginines were identified 40 years ago from acidic lysates of nuclear extracts (69). However, the first genes encoding protein arginine methyltransferases (PRMTs) were identified only a decade ago (29, 37, 54). Since this important discovery, the study of protein arginine methylation has been a rapi ...
Crk: The First Identified Adaptor Protein
... only detected in normal tissue possessing highly proliferative cells. Expression levels of Crk were examined in cultured cell lines via immunoblotting. Growth rates of the lung cancer cell lines PC-3 and NPC-8 were significantly higher than the others, and Crk expression levels were higher in these ...
... only detected in normal tissue possessing highly proliferative cells. Expression levels of Crk were examined in cultured cell lines via immunoblotting. Growth rates of the lung cancer cell lines PC-3 and NPC-8 were significantly higher than the others, and Crk expression levels were higher in these ...
Protein purification
Protein purification is a series of processes intended to isolate one or a few proteins from a complex mixture, usually cells, tissues or whole organisms. Protein purification is vital for the characterization of the function, structure and interactions of the protein of interest. The purification process may separate the protein and non-protein parts of the mixture, and finally separate the desired protein from all other proteins. Separation of one protein from all others is typically the most laborious aspect of protein purification. Separation steps usually exploit differences in protein size, physico-chemical properties, binding affinity and biological activity. The pure result may be termed protein isolate.The methods used in protein purification can roughly be divided into analytical and preparative methods. The distinction is not exact, but the deciding factor is the amount of protein that can practically be purified with that method. Analytical methods aim to detect and identify a protein in a mixture, whereas preparative methods aim to produce large quantities of the protein for other purposes, such as structural biology or industrial use. In general, the preparative methods can be used in analytical applications, but not the other way around.