Ref ID: 386
... There was a reduction of 40-70% of the MYCN protein in transfected cells compared with control cells. Real-time RT-PCR showed that MYCN mRNA levels were reduced by approximately 40% compared with the negative control cells. This experiment confirms that the reduction of MYCN protein observed on West ...
... There was a reduction of 40-70% of the MYCN protein in transfected cells compared with control cells. Real-time RT-PCR showed that MYCN mRNA levels were reduced by approximately 40% compared with the negative control cells. This experiment confirms that the reduction of MYCN protein observed on West ...
Histochemical Demonstration of Protein-Bound Alpha
... Protein-bound carboxyl groups were widespread section. For example, the staining after sublimate- in distribution in rat tissues. All epithelial cells, formol was generally more intense than after muscle fibers, and the cells of the nervous system neutral 10 per cent formalin or 80 per cent ethyl an ...
... Protein-bound carboxyl groups were widespread section. For example, the staining after sublimate- in distribution in rat tissues. All epithelial cells, formol was generally more intense than after muscle fibers, and the cells of the nervous system neutral 10 per cent formalin or 80 per cent ethyl an ...
Stockholm University
... helix in the translocon might influence (i) how efficiently it partitions into the surrounding membrane, (ii) how efficiently it interacts with more N-terminally located transmembrane helices, or (iii) how efficiently it is glycosylated on the regions flanking the transmembrane domain. In support of ...
... helix in the translocon might influence (i) how efficiently it partitions into the surrounding membrane, (ii) how efficiently it interacts with more N-terminally located transmembrane helices, or (iii) how efficiently it is glycosylated on the regions flanking the transmembrane domain. In support of ...
Regional Differences in Protein Synthesis within the Lens of
... counter. Areas under the dye-binding and radioactivity curves were determined by planimetry. Extraction with 10 per cent trichloroacetic acid (TCA) was used to determine the partition of radioactivity between protein and nonprotein constituents in two aliquots from each of the three aqueous extracts ...
... counter. Areas under the dye-binding and radioactivity curves were determined by planimetry. Extraction with 10 per cent trichloroacetic acid (TCA) was used to determine the partition of radioactivity between protein and nonprotein constituents in two aliquots from each of the three aqueous extracts ...
王红刚
... of folds without significantly affecting its performance, as long as the number of samples in each fold is not too small. ...
... of folds without significantly affecting its performance, as long as the number of samples in each fold is not too small. ...
Using the Inquiry Page in a High School Classroom
... to examine the CFTR protein sequence. In the hits page, click on P13569. Note presence of a phenylalanine residue (F) at position 508 in the CFTR sequence. There is also detailed information on various CFTR sequence mutations and their results. ...
... to examine the CFTR protein sequence. In the hits page, click on P13569. Note presence of a phenylalanine residue (F) at position 508 in the CFTR sequence. There is also detailed information on various CFTR sequence mutations and their results. ...
Effective Scoring Function for Protein Sequence Design
... rotamers of 20 amino acids. The formula was similar to that used by Kuhlman and Baker.10 In the optimization procedure, only one residue is changed at a time, whereas conformations for all other residues were kept in their native conformation. We repeated the optimization procedure four times, and t ...
... rotamers of 20 amino acids. The formula was similar to that used by Kuhlman and Baker.10 In the optimization procedure, only one residue is changed at a time, whereas conformations for all other residues were kept in their native conformation. We repeated the optimization procedure four times, and t ...
... points. On questions with choices, all of your answers will be graded and the best scoring answer will be used. Please use the space provided, or the back of the preceding page. 1. (6 pts) Please do both parts: i) Draw two “hydrogen bonded” water molecules. Indicate on your diagram a donor and an ac ...
Allantoin transport protein, PucI, from Bacillus subtilis
... DMSO to the cells prior to the energization period, the final concentration of the unlabelled compound was 500 mM and the final concentration of DMSO was 2 %. Relative uptake values were measured as a percentage of those obtained from samples in the absence of any added unlabelled compound or DMSO. ...
... DMSO to the cells prior to the energization period, the final concentration of the unlabelled compound was 500 mM and the final concentration of DMSO was 2 %. Relative uptake values were measured as a percentage of those obtained from samples in the absence of any added unlabelled compound or DMSO. ...
Purification to homogeneity and partial amino acid sequence of a
... of which will be reported elsewhere, is an adaptation of Schneider's method (31) for the measurement of DNA following its precipitation by CTAB in the presence of proteinase K, the latter removes any protein remaining in association with DNA. The assay incubation mixture contains enzyme extract made ...
... of which will be reported elsewhere, is an adaptation of Schneider's method (31) for the measurement of DNA following its precipitation by CTAB in the presence of proteinase K, the latter removes any protein remaining in association with DNA. The assay incubation mixture contains enzyme extract made ...
Gene Section NOL3 (nucleolar protein 3 (apoptosis repressor with CARD domain))
... thick boxes) and 3 small introns. The translational start site is in exon 2. Alternative splicing occurs between exons 2 and 3. This involves two splice donors separated by 10 nucleotides in exon 2 connecting to a single splice acceptor in exon 3 (Stoss et al., 1999). Because the separation between ...
... thick boxes) and 3 small introns. The translational start site is in exon 2. Alternative splicing occurs between exons 2 and 3. This involves two splice donors separated by 10 nucleotides in exon 2 connecting to a single splice acceptor in exon 3 (Stoss et al., 1999). Because the separation between ...
Slides
... packing of alphahelices, beta-sheets and random coils with respect to each other on the level of one whole polypeptide chain. ...
... packing of alphahelices, beta-sheets and random coils with respect to each other on the level of one whole polypeptide chain. ...
Oxidized Amino Acids in Lens Protein with Age
... in greater yield than DT during oxidation of model proteins in vitro. In the present studies, we have applied sensitive and specific assays for quantifying o-Tyr and DT in lens proteins using selected ion monitoring-gas chromatography/mass spectrometry (SIM-GC/MS). In addition, the total alkaline bl ...
... in greater yield than DT during oxidation of model proteins in vitro. In the present studies, we have applied sensitive and specific assays for quantifying o-Tyr and DT in lens proteins using selected ion monitoring-gas chromatography/mass spectrometry (SIM-GC/MS). In addition, the total alkaline bl ...
The CS2 fimbrial antigen from escherichia coli, purification
... have a very small a b s o r b a n c e at 280 n m which was exactly what was o b s e r v e d during the purification (Fig. 1). T h e CS2 fimbrial p r o t e i n does n o t c o n t a i n a n y cysteine residues, a n d the subunits must therefore be held together b y n o n c o v a l e n t forces. The N ...
... have a very small a b s o r b a n c e at 280 n m which was exactly what was o b s e r v e d during the purification (Fig. 1). T h e CS2 fimbrial p r o t e i n does n o t c o n t a i n a n y cysteine residues, a n d the subunits must therefore be held together b y n o n c o v a l e n t forces. The N ...
Reprogramming Cells to Fight Disease
... Bancel’s company, Moderna Therapeutics, was recently named #8 on CNBC’s Disruptor 50 list, which recognizes companies seen as revolutionaries in their respective industries. Moderna ranked ahead of such renowned disruptors as Spotify, Dropbox, Kickstarter, and Uber, and was the only biotech company ...
... Bancel’s company, Moderna Therapeutics, was recently named #8 on CNBC’s Disruptor 50 list, which recognizes companies seen as revolutionaries in their respective industries. Moderna ranked ahead of such renowned disruptors as Spotify, Dropbox, Kickstarter, and Uber, and was the only biotech company ...
SDS-PAGE strongly overestimates the molecular
... Direct molecular mass determination of the three porcine neurofilament proteins (H, M and L) was performed in 6 M guanidine-HCI using analytical gel filtration and sedimentation equilibrium centrifugation. The results show that SDS-PAGE strongly overestimates the values of the ‘higher molecular mass ...
... Direct molecular mass determination of the three porcine neurofilament proteins (H, M and L) was performed in 6 M guanidine-HCI using analytical gel filtration and sedimentation equilibrium centrifugation. The results show that SDS-PAGE strongly overestimates the values of the ‘higher molecular mass ...
template
... a great a role for input proteins, she can come up with a good solution faster if she is given the chance to group amino acids accordingly (e.g. hydrophobic, polar and charged) and then give highly negative scores between the hydrophobic group and other groups. These tasks, among other important asp ...
... a great a role for input proteins, she can come up with a good solution faster if she is given the chance to group amino acids accordingly (e.g. hydrophobic, polar and charged) and then give highly negative scores between the hydrophobic group and other groups. These tasks, among other important asp ...
Self-Interaction of the Herpes Simplex Virus Type 1
... involved in multimerization. Mutant protein R9DS13 was resolved as a 35-kDa species, but the level of expression, measured both by immunoprecipitation with polyclonal antibody and by Western blot analysis (data not shown), was extremely low compared to the wild-type protein and to the other mutant p ...
... involved in multimerization. Mutant protein R9DS13 was resolved as a 35-kDa species, but the level of expression, measured both by immunoprecipitation with polyclonal antibody and by Western blot analysis (data not shown), was extremely low compared to the wild-type protein and to the other mutant p ...
Protein Kinases - School of Medicine
... constituitively activated (it is active all of the time) • Gleevec is a specific inhibitor of the Bcr-Abl proteintyrosine kinase, and Gleevec is used therapeutically for chronic myelogenous leukemia • This malignancy is unusual because it results from a single genetic alteration; most cancers result ...
... constituitively activated (it is active all of the time) • Gleevec is a specific inhibitor of the Bcr-Abl proteintyrosine kinase, and Gleevec is used therapeutically for chronic myelogenous leukemia • This malignancy is unusual because it results from a single genetic alteration; most cancers result ...
Costs of life - Dynamics of the protein inventory of
... starvation. In total, 1,390 proteins have been identified in at least two biological replicates at one or more ...
... starvation. In total, 1,390 proteins have been identified in at least two biological replicates at one or more ...
Nucleotide sequences of genome segments $8, encoding a capsid
... of the capsid protein amino acids as described above, nevertheless seem to be serologically distinguishable. No cross-reaction was observed between RGDV and RDV (Omura et al., 1985), or between RDV and WTV (Liu & Black, 1978) when intact virus particles were used as antigens. The domains with long i ...
... of the capsid protein amino acids as described above, nevertheless seem to be serologically distinguishable. No cross-reaction was observed between RGDV and RDV (Omura et al., 1985), or between RDV and WTV (Liu & Black, 1978) when intact virus particles were used as antigens. The domains with long i ...
Nucleotide sequences of genome segments $8, encoding a capsid
... of the capsid protein amino acids as described above, nevertheless seem to be serologically distinguishable. No cross-reaction was observed between RGDV and RDV (Omura et al., 1985), or between RDV and WTV (Liu & Black, 1978) when intact virus particles were used as antigens. The domains with long i ...
... of the capsid protein amino acids as described above, nevertheless seem to be serologically distinguishable. No cross-reaction was observed between RGDV and RDV (Omura et al., 1985), or between RDV and WTV (Liu & Black, 1978) when intact virus particles were used as antigens. The domains with long i ...
S11. Computational Molecular Modeling- Week 5. 3
... ICM-Browser program and be comfortable helping students make informed choices about what regions of the proteins should be analyzed. I recommend that the instructor performs an internet background search on how protein 3-D modeling works so they understand the various types of resources that are ava ...
... ICM-Browser program and be comfortable helping students make informed choices about what regions of the proteins should be analyzed. I recommend that the instructor performs an internet background search on how protein 3-D modeling works so they understand the various types of resources that are ava ...
Summary and conclusions - Food Standards Australia New Zealand
... levels of Cry1A.105 were highest in the R6 leaf (790 µg/g dry weight) and lowest in the roots and pollen where the level was below the limit of quantitation (LOQ). For Cry2Ab2, mean protein levels were highest in the R2 – R3 leaf (32 µg/g dry weight) and were lowest in the pollen (
... levels of Cry1A.105 were highest in the R6 leaf (790 µg/g dry weight) and lowest in the roots and pollen where the level was below the limit of quantitation (LOQ). For Cry2Ab2, mean protein levels were highest in the R2 – R3 leaf (32 µg/g dry weight) and were lowest in the pollen (
Protein purification
Protein purification is a series of processes intended to isolate one or a few proteins from a complex mixture, usually cells, tissues or whole organisms. Protein purification is vital for the characterization of the function, structure and interactions of the protein of interest. The purification process may separate the protein and non-protein parts of the mixture, and finally separate the desired protein from all other proteins. Separation of one protein from all others is typically the most laborious aspect of protein purification. Separation steps usually exploit differences in protein size, physico-chemical properties, binding affinity and biological activity. The pure result may be termed protein isolate.The methods used in protein purification can roughly be divided into analytical and preparative methods. The distinction is not exact, but the deciding factor is the amount of protein that can practically be purified with that method. Analytical methods aim to detect and identify a protein in a mixture, whereas preparative methods aim to produce large quantities of the protein for other purposes, such as structural biology or industrial use. In general, the preparative methods can be used in analytical applications, but not the other way around.