Improving protein fold recognition with hybrid
... best approach for protein fold recognition. Besides these methods solely based on sequence, some authors have proposed structure-based fold recognition methods called ‘threading’ (Bowie et al., 1991; Jones et al., 1992). The principle of threading is to assess the compatibility between the target se ...
... best approach for protein fold recognition. Besides these methods solely based on sequence, some authors have proposed structure-based fold recognition methods called ‘threading’ (Bowie et al., 1991; Jones et al., 1992). The principle of threading is to assess the compatibility between the target se ...
Sugar Transport in (Hyper-)Thermophilic Archaea
... the nanomolar range are usually observed only for substrates such as vitamins and iron which are found in very low concentrations in the environment. Uptake of substrates by hyperthermophilic archaea is usually optimal around the growth temperature. ...
... the nanomolar range are usually observed only for substrates such as vitamins and iron which are found in very low concentrations in the environment. Uptake of substrates by hyperthermophilic archaea is usually optimal around the growth temperature. ...
ERNDIM - Quantitative Schemes Cystine in White Blood Cells
... especially the recovery of added cystine and protein, is fine. Of course the performance of some individual labs require improvement. The Interlab CV demonstrates lack of standardisation which requires improvement. We would like to emphasise the need for all laboratories to use internal quality cont ...
... especially the recovery of added cystine and protein, is fine. Of course the performance of some individual labs require improvement. The Interlab CV demonstrates lack of standardisation which requires improvement. We would like to emphasise the need for all laboratories to use internal quality cont ...
part 1
... • RMSD depends on protein size • Tricky to identify “equivalent residues” in the beginning • Usually means that a sequence alignment is done first • Aligned residues are considered “equivalent” • Means the method is only useful for sequences that can be aligned by sequence comparison • Several solut ...
... • RMSD depends on protein size • Tricky to identify “equivalent residues” in the beginning • Usually means that a sequence alignment is done first • Aligned residues are considered “equivalent” • Means the method is only useful for sequences that can be aligned by sequence comparison • Several solut ...
Conserved Key Amino Acid Positions (CKAAPs) Derived From the
... These substructures represent commonly identified folds, domains, or components thereof. Most of the subsequences forming these similar substructures have no significant sequence similarity. We present a method to identify conserved amino acid positions and residue-dependent property clusters within ...
... These substructures represent commonly identified folds, domains, or components thereof. Most of the subsequences forming these similar substructures have no significant sequence similarity. We present a method to identify conserved amino acid positions and residue-dependent property clusters within ...
Chapter 1 – name - Nutrition Gardener
... What is the usual fate of orally ingested enzyme supplements? a. Digested by gastrointestinal proteases b. Rapidly degraded by salivary secretions c. Mostly absorbed in original form from stomach d. Completely absorbed in original form from jejunum ...
... What is the usual fate of orally ingested enzyme supplements? a. Digested by gastrointestinal proteases b. Rapidly degraded by salivary secretions c. Mostly absorbed in original form from stomach d. Completely absorbed in original form from jejunum ...
Types of Nutrients
... If you eat more than you need for these purposes, the extra protein is used for energy. The image below shows how many grams of protein you need each day ( Figure 1.3). It also shows some foods that are good sources of protein. Lipids ...
... If you eat more than you need for these purposes, the extra protein is used for energy. The image below shows how many grams of protein you need each day ( Figure 1.3). It also shows some foods that are good sources of protein. Lipids ...
Protein reutilisation in corms of Colchicum autumnale
... M HCl) and 1 mL of N-(1-Naphthyl)-ethylendiamine dihydrochloride (0.02% in distilled water). The absorbance of azo dye was measured at 540 nm. Total nitrogen and total amino acid content Total nitrogen assay was estimated using Kjeldahl method (FERENČÍK et al., 1981). The spectrum and level of total ...
... M HCl) and 1 mL of N-(1-Naphthyl)-ethylendiamine dihydrochloride (0.02% in distilled water). The absorbance of azo dye was measured at 540 nm. Total nitrogen and total amino acid content Total nitrogen assay was estimated using Kjeldahl method (FERENČÍK et al., 1981). The spectrum and level of total ...
Critical care: Meeting protein requirements without overfeeding energy
... international guidelines. We determined protein adequacy of current feed prescription in a ‘baseline’ audit and then the effect of added protein in a pilot ‘supplementation’ audit. The primary outcome was the difference between protein prescription and the local guidelines on days 1e3. Secondary out ...
... international guidelines. We determined protein adequacy of current feed prescription in a ‘baseline’ audit and then the effect of added protein in a pilot ‘supplementation’ audit. The primary outcome was the difference between protein prescription and the local guidelines on days 1e3. Secondary out ...
Supplementary materials
... protein gels. Left panel: 2-D patterns of the soluble-fraction proteins isolated from S. oneidensis MR-1 after whole-cell lysate. Proteins were separated by two-dimensional gel electrophoresis with isoeletric focusing in the first dimension and sodium dodecyl sulfate electrophoresis in the second di ...
... protein gels. Left panel: 2-D patterns of the soluble-fraction proteins isolated from S. oneidensis MR-1 after whole-cell lysate. Proteins were separated by two-dimensional gel electrophoresis with isoeletric focusing in the first dimension and sodium dodecyl sulfate electrophoresis in the second di ...
Degradation signals within both terminal domains of the cauliflower
... Introduction Protein degradation plays an important role in many cellular processes: it allows much faster alteration of the amount of regulatory proteins than transcriptional or translational regulation, and is important for the relocation of biochemical resources. Although protein degradation has ...
... Introduction Protein degradation plays an important role in many cellular processes: it allows much faster alteration of the amount of regulatory proteins than transcriptional or translational regulation, and is important for the relocation of biochemical resources. Although protein degradation has ...
IJEB 48(4) 373-377
... revealed that it contained Pro, Leu/Ile, Val and Gly as major components and Glu, Phe, Ser, Ala and Asp as minor components19. Terayama and Takeuchi20 isolated a fraction on hydrolysis of azo-dye binding protein which was found to have Phe, Ser, Gly, Pro, Val, Glu and Asp. The discrepancies between ...
... revealed that it contained Pro, Leu/Ile, Val and Gly as major components and Glu, Phe, Ser, Ala and Asp as minor components19. Terayama and Takeuchi20 isolated a fraction on hydrolysis of azo-dye binding protein which was found to have Phe, Ser, Gly, Pro, Val, Glu and Asp. The discrepancies between ...
Printer Friendly Document
... * Go to STRING, BLAST, select Deinococcus geothermalis * Note clustering with 3 enzymes of NAD synthesis and 5 enzymes of thiamin synthesis (Similar but not identical results with D. deserti or D. radiodurans. Note that it is important to try more than one organism as an entry point to the system.) ...
... * Go to STRING, BLAST, select Deinococcus geothermalis * Note clustering with 3 enzymes of NAD synthesis and 5 enzymes of thiamin synthesis (Similar but not identical results with D. deserti or D. radiodurans. Note that it is important to try more than one organism as an entry point to the system.) ...
Functional analysis of the Drosophila Dnop5 gene using targeted RNA interference
... RNAi is a cellular process of gene silencing in which small duplexes of RNA specifically target a homologous sequence for cleavage, and has proven to be a powerful tool for disrupting gene expression. This technique was initially developed in plants and nematodes and more recently in Drosophila, Zeb ...
... RNAi is a cellular process of gene silencing in which small duplexes of RNA specifically target a homologous sequence for cleavage, and has proven to be a powerful tool for disrupting gene expression. This technique was initially developed in plants and nematodes and more recently in Drosophila, Zeb ...
JOURNAL OF APPLIED SCIENCE AND AGRICULTURE
... content among many other beneficial minerals and nutrients. Recently there are a few novel water-based extraction methods introduced such as subcritical and hydrothermal extraction. However simpler methods of water extract such as autoclaving and sonication can produce comparable results. Therefore ...
... content among many other beneficial minerals and nutrients. Recently there are a few novel water-based extraction methods introduced such as subcritical and hydrothermal extraction. However simpler methods of water extract such as autoclaving and sonication can produce comparable results. Therefore ...
Predicted function of the vaccinia virus G5R protein
... each protein associated with the intracellular mature virion using mass spectrometry were unable to detect G5R within the intracellular mature virion (Yoder et al., 2006; Chung et al., 2006). When we employed a hidden Markov model (HMM) comparison search tool (HHsearch) to screen for the functions o ...
... each protein associated with the intracellular mature virion using mass spectrometry were unable to detect G5R within the intracellular mature virion (Yoder et al., 2006; Chung et al., 2006). When we employed a hidden Markov model (HMM) comparison search tool (HHsearch) to screen for the functions o ...
Predicting protein folding rates from geometric contact and amino
... predict the folding rates for proteins of diverse classes based only on the amino acid sequence of the protein, without knowledge of the tertiary or secondary structures, or information of structural class, and without the aid of any other computational prediction of structural properties (e.g., sec ...
... predict the folding rates for proteins of diverse classes based only on the amino acid sequence of the protein, without knowledge of the tertiary or secondary structures, or information of structural class, and without the aid of any other computational prediction of structural properties (e.g., sec ...
High pressure effects on protein structure and function
... recent indications7 for the existence of pressure-induced predenaturation transitions, which in turn point to step-wise processes. In some respect, pressure denaturation studies can be also more straightforward, in comparison with denaturation induced by guanidinium chloride or urea at atmospheric p ...
... recent indications7 for the existence of pressure-induced predenaturation transitions, which in turn point to step-wise processes. In some respect, pressure denaturation studies can be also more straightforward, in comparison with denaturation induced by guanidinium chloride or urea at atmospheric p ...
GENE NOMENCLATURE OF DIFFERENT TYPES OF SPINOCEREBELLAR ATAXIA (SCA) AND
... distinct genetic causes of SCA are known and each of which could be considered a disease in its own right. The nomenclature of 36 types of Spinocerebellar ataxia and a computational work on Spinocerebellar ataxia type 1 (SCA1) are done in this study. SCA1 is a progressive, degenerative and often fat ...
... distinct genetic causes of SCA are known and each of which could be considered a disease in its own right. The nomenclature of 36 types of Spinocerebellar ataxia and a computational work on Spinocerebellar ataxia type 1 (SCA1) are done in this study. SCA1 is a progressive, degenerative and often fat ...
Constitutive heat shock protein 70 (HSC70) expression in rainbow
... cells. The constitutive member of the 70 family (hsc70) is crucial for the chaperoning function of unstressed cells, whereas the inducible form (hsp70) is important for allowing cells to cope with acute stressor insult, especially those affecting the protein machinery. In fish, the role of hsc70 in ...
... cells. The constitutive member of the 70 family (hsc70) is crucial for the chaperoning function of unstressed cells, whereas the inducible form (hsp70) is important for allowing cells to cope with acute stressor insult, especially those affecting the protein machinery. In fish, the role of hsc70 in ...
EXPRESSO® Cloning and Expression Systems
... PCR cloning strategy that is directional, eliminates the need for restriction digestion and ligation, and requires no PCR reaction clean-up or enzyme treatment. In the Expresso T7 System, pre-processed pETite® expression vectors allow one-step cloning of target gene PCR products under the control of ...
... PCR cloning strategy that is directional, eliminates the need for restriction digestion and ligation, and requires no PCR reaction clean-up or enzyme treatment. In the Expresso T7 System, pre-processed pETite® expression vectors allow one-step cloning of target gene PCR products under the control of ...
A Method to Identify Protein Sequences that Fold into a Known
... A more direct attack on the inverse protein folding problem was taken by Ponder and Richards ( S ) , who adopted quite literally the suggestion of Drexler ( 9 ) and Pabo (10) that one should search for sequences that are compatible with a given structure. In their "tertiary template" method, the bac ...
... A more direct attack on the inverse protein folding problem was taken by Ponder and Richards ( S ) , who adopted quite literally the suggestion of Drexler ( 9 ) and Pabo (10) that one should search for sequences that are compatible with a given structure. In their "tertiary template" method, the bac ...
Binding of a Growth Hormone- Inducible Nuclear Factor Is Mediated
... despite the absence of immunoreactivity to N-terminal p91 antibodies. The signalingpathways of growth factor activation of Ras proteins, followed by activation of a kinase cascade, may also be activated by GH. It has been demonstrated that mitogen-activatedprotein (MAP) kinases are part of the phosp ...
... despite the absence of immunoreactivity to N-terminal p91 antibodies. The signalingpathways of growth factor activation of Ras proteins, followed by activation of a kinase cascade, may also be activated by GH. It has been demonstrated that mitogen-activatedprotein (MAP) kinases are part of the phosp ...
Cardiovascular 19 – Homeostasis and Thrombosis
... Antithrombin is an inhibitor of coagulation factors such as Xa, XIa, IXa and thrombin. Its activity is enhanced by heparin sulphates. A drug Heparin enhances antithrombin’s actions. II. Indirect inhibition by protein C Thrombin binds to receptor on endothelial cell called thrombomodulin. This the ac ...
... Antithrombin is an inhibitor of coagulation factors such as Xa, XIa, IXa and thrombin. Its activity is enhanced by heparin sulphates. A drug Heparin enhances antithrombin’s actions. II. Indirect inhibition by protein C Thrombin binds to receptor on endothelial cell called thrombomodulin. This the ac ...
Protein purification
Protein purification is a series of processes intended to isolate one or a few proteins from a complex mixture, usually cells, tissues or whole organisms. Protein purification is vital for the characterization of the function, structure and interactions of the protein of interest. The purification process may separate the protein and non-protein parts of the mixture, and finally separate the desired protein from all other proteins. Separation of one protein from all others is typically the most laborious aspect of protein purification. Separation steps usually exploit differences in protein size, physico-chemical properties, binding affinity and biological activity. The pure result may be termed protein isolate.The methods used in protein purification can roughly be divided into analytical and preparative methods. The distinction is not exact, but the deciding factor is the amount of protein that can practically be purified with that method. Analytical methods aim to detect and identify a protein in a mixture, whereas preparative methods aim to produce large quantities of the protein for other purposes, such as structural biology or industrial use. In general, the preparative methods can be used in analytical applications, but not the other way around.