投影片 1
... induction indicates transcriptional activity compared with pIRES vector control plasmid (right panel). (C) HEK293 cells were transfected with increasing concentration of GSKIP as indicated. Each value represents the mean ± SD of three separate experiments. Statistically significant differences as de ...
... induction indicates transcriptional activity compared with pIRES vector control plasmid (right panel). (C) HEK293 cells were transfected with increasing concentration of GSKIP as indicated. Each value represents the mean ± SD of three separate experiments. Statistically significant differences as de ...
Notes for using PROTPOL.f
... (note that the RESTYPE and NORES for the CA, C, O are incorrectly given as those for the following residue – these parameters are only correct for the N) Followed by three lines with (Q(I, I), (H(J, I), J = 1, 3), I = 1, 3) Q (I, I) = Ith diagonal element of moment of inertia matrix in principal axi ...
... (note that the RESTYPE and NORES for the CA, C, O are incorrectly given as those for the following residue – these parameters are only correct for the N) Followed by three lines with (Q(I, I), (H(J, I), J = 1, 3), I = 1, 3) Q (I, I) = Ith diagonal element of moment of inertia matrix in principal axi ...
the simple quad method - New Moon Family Acupuncture
... Carbohydrate, and a small amount of Fat. Your serving size for the entire meal should be about the size of your two hands cupped together. This is the amount that your stomach can comfortably handle at each meal. An example would be baked chicken, cooked carrots, salad and a small baked potato. Or g ...
... Carbohydrate, and a small amount of Fat. Your serving size for the entire meal should be about the size of your two hands cupped together. This is the amount that your stomach can comfortably handle at each meal. An example would be baked chicken, cooked carrots, salad and a small baked potato. Or g ...
TRYPSIN / LYS
... arginine residues. Unmodified trypsin is subject to autolysis, generating fragments that can interfere with protein sequencing, HPLC or mass spectrometry analysis of the peptides. In addition, autolysis can result in the generation of pseudotrypsin, which has been shown to exhibit an additional chym ...
... arginine residues. Unmodified trypsin is subject to autolysis, generating fragments that can interfere with protein sequencing, HPLC or mass spectrometry analysis of the peptides. In addition, autolysis can result in the generation of pseudotrypsin, which has been shown to exhibit an additional chym ...
DIET for HEIGHT
... Though the body height is determined genetically, it is also a well-‐known fact that growth depends on certain external factors and nutrition is one of them. Poor height can be a result of slow gr ...
... Though the body height is determined genetically, it is also a well-‐known fact that growth depends on certain external factors and nutrition is one of them. Poor height can be a result of slow gr ...
Classifier ensembles for protein structural class prediction with
... prediction by using alternative sequence representations. Examples include auto-correlation functions based on non-bonded residue energy [7], polypeptide composition [26,37,44], and functional domain composition [16]. However, these algorithms are often only tested on very small datasets, with uncon ...
... prediction by using alternative sequence representations. Examples include auto-correlation functions based on non-bonded residue energy [7], polypeptide composition [26,37,44], and functional domain composition [16]. However, these algorithms are often only tested on very small datasets, with uncon ...
1 A Comparative, Double-blind, Triple Crossover Net Nitrogen
... Due to the fact that the methodology of the nitrogen balance is valid only when it is carried out in a accurate manner (8,9), the following precautions were taken during the study: a) To avoid or to reduce to a minimum the possible differences in the retention of N, caused by a determined dietetic s ...
... Due to the fact that the methodology of the nitrogen balance is valid only when it is carried out in a accurate manner (8,9), the following precautions were taken during the study: a) To avoid or to reduce to a minimum the possible differences in the retention of N, caused by a determined dietetic s ...
Solid Tumour Section Soft tissue tumors: Extraskeletal myxoid chondrosarcoma
... shown by positive immunohistochemical reactivities to neural or neuroendocrine markers such as neuron-specific enolase, synaptophysin, chromogranin A, and PGP9.5; tumours are mostly negative for markers (collagen type II, X, proteoglycan aggrecan) for the chondrocytic cell lineage. Ultrastructurally ...
... shown by positive immunohistochemical reactivities to neural or neuroendocrine markers such as neuron-specific enolase, synaptophysin, chromogranin A, and PGP9.5; tumours are mostly negative for markers (collagen type II, X, proteoglycan aggrecan) for the chondrocytic cell lineage. Ultrastructurally ...
Improved detection and identification of low
... over 20 h. IPG strips can also be run on IPGphor™ electrophoresis unit using paper bridges on Cup Loading Strip Holder, which achieves equally high spot resolution. All chemicals and reagents used for the second dimension of 2-D electrophoresis are described in reference 6. Initial equilibration of ...
... over 20 h. IPG strips can also be run on IPGphor™ electrophoresis unit using paper bridges on Cup Loading Strip Holder, which achieves equally high spot resolution. All chemicals and reagents used for the second dimension of 2-D electrophoresis are described in reference 6. Initial equilibration of ...
Printer Friendly Document
... * Go to STRING, BLAST, select Deinococcus geothermalis * Note clustering with 3 enzymes of NAD synthesis and 5 enzymes of thiamin synthesis (Similar but not identical results with D. deserti or D. radiodurans. Note that it is important to try more than one organism as an entry point to the system.) ...
... * Go to STRING, BLAST, select Deinococcus geothermalis * Note clustering with 3 enzymes of NAD synthesis and 5 enzymes of thiamin synthesis (Similar but not identical results with D. deserti or D. radiodurans. Note that it is important to try more than one organism as an entry point to the system.) ...
NIH Public Access
... (2 mL/min) until the absorbances at 280 nm returned to baseline. GST-AID was eluted with the same buffer but including 20 mM glutathione and pH 8.0. Fractions containing the GSTAID construct were combined and concentrated to ∼0.5 mL using an Amicon ultrafiltration concentrators (5 kDa molecular weig ...
... (2 mL/min) until the absorbances at 280 nm returned to baseline. GST-AID was eluted with the same buffer but including 20 mM glutathione and pH 8.0. Fractions containing the GSTAID construct were combined and concentrated to ∼0.5 mL using an Amicon ultrafiltration concentrators (5 kDa molecular weig ...
mechanism of the flagellar export system and its potential
... complete highly conserved N-terminal part of the molecule, and the first report demonstrating that the N-terminal part of flagellin contains the signal used about the same fragment size. These 18 constructs were expressed in a flagellin-deficient S. typhimurium strain and the export of the given fus ...
... complete highly conserved N-terminal part of the molecule, and the first report demonstrating that the N-terminal part of flagellin contains the signal used about the same fragment size. These 18 constructs were expressed in a flagellin-deficient S. typhimurium strain and the export of the given fus ...
emboj200897-sup
... commonly quantified by SDS-PAGE gel autoradiography, with the (specifically mutated) PTK itself as the substrate (Soulat et al., 2007). While the PTK serves as both the kinase and the substrate (hence the autokinase activity is measured), it is important to control the initial phosphorylation level ...
... commonly quantified by SDS-PAGE gel autoradiography, with the (specifically mutated) PTK itself as the substrate (Soulat et al., 2007). While the PTK serves as both the kinase and the substrate (hence the autokinase activity is measured), it is important to control the initial phosphorylation level ...
PrP
... Pathologic specimen from a patient with CJD demonstrating spongiform changes and neuronal loss. ...
... Pathologic specimen from a patient with CJD demonstrating spongiform changes and neuronal loss. ...
Circadian Rhythm of Intestinal SucraseActivity in Rats
... of 0.5 mg/ml after 60 min of incubation with papain and its inclusion during immunoprecipitation did not affect the pattern found on sodium dodecyl sulfate-acrylamide gel electrophoresis of immunoprecipitates, so that a protease inhibitor was not used.) Resultant supernatant fractions were dialyzed ...
... of 0.5 mg/ml after 60 min of incubation with papain and its inclusion during immunoprecipitation did not affect the pattern found on sodium dodecyl sulfate-acrylamide gel electrophoresis of immunoprecipitates, so that a protease inhibitor was not used.) Resultant supernatant fractions were dialyzed ...
Database Modeling in Bioinformatics
... Standardized transfer of annotation from characterized proteins in SWISS-PROT to TrEMBL entries • TrEMBL entry is reliably recognized by a given method as a member of a certain group of proteins • corresponding group of proteins in SWISS-PROT shares certain annotation • common annotation is transfe ...
... Standardized transfer of annotation from characterized proteins in SWISS-PROT to TrEMBL entries • TrEMBL entry is reliably recognized by a given method as a member of a certain group of proteins • corresponding group of proteins in SWISS-PROT shares certain annotation • common annotation is transfe ...
Analysis of Proteins
... They are water soluble b/c the polar R groups are on the outer surface where they can interact with water. ...
... They are water soluble b/c the polar R groups are on the outer surface where they can interact with water. ...
This presentation introduces the topics we will
... used. The resulting mixture of peptides is analysed by mass spectrometry. This yields a set of molecular mass values, which are searched against a database of protein sequences using a search engine. For each entry in the protein database, the search engine simulates the known cleavage specificity o ...
... used. The resulting mixture of peptides is analysed by mass spectrometry. This yields a set of molecular mass values, which are searched against a database of protein sequences using a search engine. For each entry in the protein database, the search engine simulates the known cleavage specificity o ...
107105_pku
... ~125,000 base pairs codes for a protein of 454 amino acids only 1362 base pairs code for amino ac ids 13 exons containing 41-184 base pairs coding for protein 12 introns from 1, 200 to 23,500 base pairs in length ...
... ~125,000 base pairs codes for a protein of 454 amino acids only 1362 base pairs code for amino ac ids 13 exons containing 41-184 base pairs coding for protein 12 introns from 1, 200 to 23,500 base pairs in length ...
Sample Preparation 2D-GE
... gels are suggested to help reduce keratin contamination. Pre-cast gels from BioRad are used routinely at the BPF. 2. Fresh staining reagents should be used (contaminants build up in re-used Coomassie stain). Silver staining and Coomassie staining are both compatible with mass spectrometry-based prot ...
... gels are suggested to help reduce keratin contamination. Pre-cast gels from BioRad are used routinely at the BPF. 2. Fresh staining reagents should be used (contaminants build up in re-used Coomassie stain). Silver staining and Coomassie staining are both compatible with mass spectrometry-based prot ...
Recombinant N-terminal Nucleotide
... nucleotide-binding domains. An alternative was to use a hexahistidine tag for fusion, in order to increase protein solubility and allow its purification by nickel-chelate chromatography. The aim of the present work was to design the N-terminal nucleotide-binding domain (NBD1) from mouse P-glycoprote ...
... nucleotide-binding domains. An alternative was to use a hexahistidine tag for fusion, in order to increase protein solubility and allow its purification by nickel-chelate chromatography. The aim of the present work was to design the N-terminal nucleotide-binding domain (NBD1) from mouse P-glycoprote ...
The proteome of Saccharomyces cerevisiae mitochondria
... itochondria play a central role in many cellular functions, including bioenergetics, apoptosis, and the metabolism of amino acids, lipids, and iron (1–4). Many diseases have been attributed to mitochondrial defects (5–8). According to the currently available information, however, only ⬇50–60% of all ...
... itochondria play a central role in many cellular functions, including bioenergetics, apoptosis, and the metabolism of amino acids, lipids, and iron (1–4). Many diseases have been attributed to mitochondrial defects (5–8). According to the currently available information, however, only ⬇50–60% of all ...
18.3 Amino Acids - Haverford Alchemy
... 18.8 Shape-Determining Interactions in Proteins Protein Analysis by Electrophoresis • Protein molecules in solution can be separated from each other by taking advantage of their net charges. • In the electric field between two electrodes, a positively charged particle moves toward the negative elec ...
... 18.8 Shape-Determining Interactions in Proteins Protein Analysis by Electrophoresis • Protein molecules in solution can be separated from each other by taking advantage of their net charges. • In the electric field between two electrodes, a positively charged particle moves toward the negative elec ...
Exploring your protein - QIAGEN Bioinformatics
... can be used as input to the tools found in the Sequence Analysis folder in the Toolbox. Many of the tools will add annotation to the sequence. If the link between sequence and structure is maintained, the sequence annotations can conveniently be visualized in the protein structure context, by simply ...
... can be used as input to the tools found in the Sequence Analysis folder in the Toolbox. Many of the tools will add annotation to the sequence. If the link between sequence and structure is maintained, the sequence annotations can conveniently be visualized in the protein structure context, by simply ...
Protein purification
Protein purification is a series of processes intended to isolate one or a few proteins from a complex mixture, usually cells, tissues or whole organisms. Protein purification is vital for the characterization of the function, structure and interactions of the protein of interest. The purification process may separate the protein and non-protein parts of the mixture, and finally separate the desired protein from all other proteins. Separation of one protein from all others is typically the most laborious aspect of protein purification. Separation steps usually exploit differences in protein size, physico-chemical properties, binding affinity and biological activity. The pure result may be termed protein isolate.The methods used in protein purification can roughly be divided into analytical and preparative methods. The distinction is not exact, but the deciding factor is the amount of protein that can practically be purified with that method. Analytical methods aim to detect and identify a protein in a mixture, whereas preparative methods aim to produce large quantities of the protein for other purposes, such as structural biology or industrial use. In general, the preparative methods can be used in analytical applications, but not the other way around.