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PCR – polymerace chain reaction
PCR – polymerace chain reaction

...  No harm (for binding) of one or two mismatches  Primers can be designed to contain errors  Binding is not disturbed SILENT MUTATION: one base is placed by another base, witch won’t change amino acid sequence ...
DNA Workshop - Mrs. Sills` Science Site
DNA Workshop - Mrs. Sills` Science Site

... Click “ok” and match the mRNA nucleotides with their complementary base pairs on the DNA by moving the bases with your mouse over to the DNA. 16. Which nucleotide is found only in RNA? _________________________________________. 17. What is the complementary base pair for this nucleotide? __________ ...
Name: Block: ______ How Does DNA Determine the Traits of an
Name: Block: ______ How Does DNA Determine the Traits of an

... If the AAC codon on the 3rd gene underwent a Substitution mutation, and the new DNA strand read: TTTAAAAAA ...
Albert Libchaber Detlev W. Bronk Professor The Rockefeller
Albert Libchaber Detlev W. Bronk Professor The Rockefeller

... In the fascinating puzzle of the origin of life, two main phenomena distinguish biology from non-equilibrium thermodynamic processes: the presence of a code and the ability of machines to self-reproduce. - In the RNA world of the early soup we are studying how a genetic code could originate, buildin ...
RNA PP
RNA PP

... • During transcription, RNA polymerase binds to DNA and separates the DNA strands. RNA polymerase then uses one strand of DNA as a template from which nucleotides are assembled into a strand of RNA. • So, RNA is making a single-stranded copy from DNA that takes information out of the ...
2421 _Ch8.ppt
2421 _Ch8.ppt

... Bacterial Chromosome Bacteria generally have a single, circular chromosome e.g. E. coli chromosome consists of 4 million base pairs (4 x 106 bp) this is a medium sized bacterial chromosome if the average gene is 1000 bp in size, then this chromosome would contain an estimated 4,000 genes ...
Multiple Choice - saddlespace.org
Multiple Choice - saddlespace.org

... a. each with two new strands. b. one with two new strands and the other with two original strands. c. each with one new strand and one original strand. d. each with two original strands. ____ 4. During mitosis, the a. DNA molecules unwind. b. histones and DNA molecules separate. c. DNA molecules bec ...
Syllabus Checklist
Syllabus Checklist

... Mitochondrial DNA is believed to control the production of proteins which are involved in respiration. Which sex contributes this DNA to the offspring? ...
Exam I Cell and Molecular Biology September 26, 2007 This exam
Exam I Cell and Molecular Biology September 26, 2007 This exam

... Because DNA is the molecule of inheritance, replication errors must be carefully avoided. Base pairing without a 3´-OH primer, as necessarily performed by any RNA polymerase, is very error prone. If a DNA polymerase performed this function, errors in the DNA sequence (mutations) would be introduced ...
Restriction Analysis of pARA and pKAN-R
Restriction Analysis of pARA and pKAN-R

... Capable of self replication May contain an antibiotic resistant gene(s) and/or other gene(s) ...
The Nature of Matter
The Nature of Matter

...  Made up of carbon, hydrogen, and oxygen atoms (C, H, O)  Hydrogen and carbon will be in a 2:1 ratio  Used by cells as the main source of energy.  Plants and some animals use carbohydrates in structures.  Examples: Starches and sugars, such as glucose (C6H12O6) and cellulose (in plant ...
Genomics
Genomics

... Sequence of All of the DNA in a Human Cell ...
Chapter 10B: Gene Expression
Chapter 10B: Gene Expression

... ( the “end products” for a small percentage of genes are special types of RNA molecules) ...
DNA!
DNA!

... Some disorders that can be tested using DNA screening are: 1. Down Syndrome – tested using an amniocentesis which allows a lab tech to create a karyotype (a map of chromosomes) to determine if trisomy 21 (3 chromosomes at 21 instead of 2) occurs. 2. PKU – tested using a blood sample to look for the ...
Gene Expression
Gene Expression

... responses to stimuli in the environment to keep you alive. For example, when you are frightened, the DNA tells your adrenal cells to produce adrenaline which will help you respond to the situation. ...
Microbial Genetics
Microbial Genetics

... when a parent cell divides. • Gene product refers to the actual structures, enzymes or regulators. • Most gene products will be a polypeptide, which fold-up into functions proteins. • The instructions are manifest as a unique sequence of nucleotide base pairs within a larger DNA molecule. • A univer ...
國立嘉義大學九十一學年度
國立嘉義大學九十一學年度

... 1.The region of DNA that is upstream from a prokaryotic gene(s) and to which a repressor or activator binds. 2.The entire complement of genetic material of an organism, virus, or organelle. 3.A DNA sequence that is used to detect the presence of a complementary sequence by hybridization with a nucle ...
Genetic Engineering (and other cool molecular biology techniques)
Genetic Engineering (and other cool molecular biology techniques)

... capable of being transformed) • Treat those cells to calcium chloride • Heat shock to force bacterial cell to take up plasmid DNA ...
Slide 1
Slide 1

...  DNA is made up of monomers called nucleotides  each nucleotide is made up of three parts: 1. 5-carbon sugar (deoxyribose) 2. phosphate group 3. nitrogenous base  FOUR types of nitrogenous bases adenine (A) & guanine (G) – purines cytosine (C) & thymine (T) – pyrimidines ...
Annexure `CD-01` L T P/S SW/FW TOTAL CREDIT UNITS 3 0 0 0 3
Annexure `CD-01` L T P/S SW/FW TOTAL CREDIT UNITS 3 0 0 0 3

... Nucleic acids Difference between DNA and RNA. Classification of RNA. Biosynthesis of DNA: Replication. ...
The Genetic Code is Read in Three Bases at a Time
The Genetic Code is Read in Three Bases at a Time

... combinations. Also, analysis of the amino acid sequence of “mutant” proteins produced a result even more damaging to Gamow’s hypothesis: a single mutation typically produced a protein with only a single amino acid different from normal, while an overlapping code would predict that three adjacent ami ...
Chapter 6 Review Terms: Somatic Cell, Game - District 196 e
Chapter 6 Review Terms: Somatic Cell, Game - District 196 e

... A  pairs  with  _____,  C  pairs  with  _____,  G  pairs  with  _____,  and  T  pairs  with  _____   ...
In the DNA Double Helix, complementary base pairs are held
In the DNA Double Helix, complementary base pairs are held

... A. the electron microscope, the human eye, and the light microscope. B. the light microscope, the electron microscope, and the human eye. C. the scanning electron microscope, the transmission electron microscope, and the light microscope. D. the human eye, the light microscope, and the electron micr ...
Lesson Plan
Lesson Plan

... 6C(S): SWBAT explain the purpose and process of transcription and translation using models of DNA and RNA. 6E Identify and illustrate changes in DNA and evaluate the significance of these changes. ...
A rapid method for isolating high quality plasmid
A rapid method for isolating high quality plasmid

... columns and state that these may need to be run more than once3. Both of these extra procedures add to the expense, and are time-consuming. They are not required in the method we describe here. Our method yields high-quality DNA that can* be readily sequenced by the dideoxy chain termination method ...
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Nucleic acid analogue



Nucleic acid analogues are compounds which are analogous (structurally similar) to naturally occurring RNA and DNA, used in medicine and in molecular biology research.Nucleic acids are chains of nucleotides, which are composed of three parts: a phosphate backbone, a pucker-shaped pentose sugar, either ribose or deoxyribose, and one of four nucleobases.An analogue may have any of these altered. Typically the analogue nucleobases confer, among other things, different base pairing and base stacking properties. Examples include universal bases, which can pair with all four canonical bases, and phosphate-sugar backbone analogues such as PNA, which affect the properties of the chain (PNA can even form a triple helix).Nucleic acid analogues are also called Xeno Nucleic Acid and represent one of the main pillars of xenobiology, the design of new-to-nature forms of life based on alternative biochemistries.Artificial nucleic acids include peptide nucleic acid (PNA), Morpholino and locked nucleic acid (LNA), as well as glycol nucleic acid (GNA) and threose nucleic acid (TNA). Each of these is distinguished from naturally occurring DNA or RNA by changes to the backbone of the molecule.In May 2014, researchers announced that they had successfully introduced two new artificial nucleotides into bacterial DNA, and by including individual artificial nucleotides in the culture media, were able to passage the bacteria 24 times; they did not create mRNA or proteins able to use the artificial nucleotides. The artificial nucleotides featured 2 fused aromatic rings.
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