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CHAPTER 18
CHAPTER 18

... concentration of ddNTPs (dideoxyribonucleoside triphosphates), different one in each sample. – DNA fragments of different lengths are synthesized in each sample, with synthesis terminating where ddNTP has been randomly incorporated. ...
Please read the following information in your groups. Make sure you
Please read the following information in your groups. Make sure you

... • Only a few mutated cells that do survive lose their ability to maintain normal cell growth. • Potentially cancerous cells are often destroyed by the body’s immune system. • DNA and its associated repair enzymes have a precise self-checking system that cuts and repairs any abnormal DNA segments bef ...
Evolution: Mutation
Evolution: Mutation

... The mutation which occurs during the removal of chromosomal DNA is called a deletion of genes. An inversion happens when a section of a chromosome rotates, but the genes are still present. A translocation occurs when a section of chromosome breaks and relocates itself to a different chromosome. A su ...
Kids Building Bricks - Johnston County Schools
Kids Building Bricks - Johnston County Schools

... • From DNA to mRNA • Occurs in the nucleus • Enzymes make a RNA copy of a segment of DNA –Just like DNA replication except A pairs with U, not with T ...
the genetic material
the genetic material

... an RNA polymerase molecule binds, which initiates the transcription of a specific gene  Termination Signal: a specific sequence of nucleotides that marks the end of a gene  Genetic Code: the rule that describes how a sequence of nucleotides, read in groups of three consecutive nucleotides (triplet ...
Document
Document

... modifications of the histones loosen DNA binding ...
highly specific nucleases for gene targeting and
highly specific nucleases for gene targeting and

Chapter 18 Gene Expression and Protein Synthesis
Chapter 18 Gene Expression and Protein Synthesis

... one of which is the promoter. A promoter is unique to each gene. • There is always a sequence of bases on the DNA strand called an initiation signal. • Promoters also contain consensus sequences, such as the TATA box, in which the two nucleotides T and A are repeated many times. ...
5` 3`
5` 3`

... - presence of long introns (& short exons) can make finding genes in eukaryotic DNA sequences difficult - may be alternative splicing pathways so more than one protein generated from one gene (Discussed later, Chapter 6) Fig.1.13 ...
MUTATIONS
MUTATIONS

... chunk from one side of the DNA containing the mistake. Then new enzymes, using the base pairing code, make a new side from the opposite strand of DNA.  The new strand is put into place by another enzyme system. ...
PAPER 1 File
PAPER 1 File

... Which of the following is a correct comparison of the bryophytes and the filicinophytes? Bryophytes ...
Exam #1
Exam #1

Daily Warm Ups, Q1
Daily Warm Ups, Q1

... • What occurs during Anaphase of Mitosis? ...
From Gene to Protein
From Gene to Protein

... While the mechanisms of translocation vary, each of these polypeptides has a “postal” code that ensures its delivery to the correct cellular location. ...
Viruses
Viruses

... Retroviruses Mutations are common No vaccines or new ones have to be made yearly. Ex: influenza, HIV, Ebola ...
Chapter 8 Microbial Genetics
Chapter 8 Microbial Genetics

... Explain the differences between: photoheteotrophs and photoautotrophs , and provide named (Genus and species) examples of each. ...
Genetics: The Science of Heredity
Genetics: The Science of Heredity

BLOOD GROUP GENOTYPING: THE FUTURE IS NOW
BLOOD GROUP GENOTYPING: THE FUTURE IS NOW

... Traditionally has been done by phenotyping using serological methods Can now be done by genotyping (DNA) ...
Bench Guide
Bench Guide

... (rRNA) and proteins. Amino acids for protein synthesis are delivered to the ribosome on transfer RNA (tRNA) molecules. RNAs are also part of riboproteins involved in RNA processing. In addition, many viruses contain RNA as their genome instead of DNA, and RNA species called ribozymes catalyze bioche ...
concepts of matter and energy
concepts of matter and energy

biochemistry
biochemistry

... • This gives the 2 hydrogen ends of the water molecule a slightly positive charge, and the oxygen end, a slightly negative charge. This is not a true charged particle or ion because the electrons are not transferred; they are still shared, just not equally. At any given moment the electrons tend to ...
A new method of finding similarity regions in DNA sequences
A new method of finding similarity regions in DNA sequences

... on searching for small exact repetitions of fixed size (seeds) and trying to extend those into larger approximate repeats. BLAST family [1] is the most prominent representative of this approach. ASSIRC [7] is another example. A slightly different but related method is implemented in FASTA [6]. REPut ...
Mutations Terminology
Mutations Terminology

... CG or GC ...
Slide 1
Slide 1

... Cloning, in theory, allows you to turn any cell into an animal. So instead of injecting DNA into an egg, you can shoot DNA into cells in a petri dish, allow them to grow and look among millions of cells for the type of genetic alteration you want. Since it is so much easier to manipulate cells than ...


... To clone a gene, a vector is first prepared. To genetically engineer a plasmid or virus, restriction enzymes are used to cleave plasmid DNA and foreign DNA. The "sticky ends" produced facilitate the insertion of foreign DNA into vector DNA. The foreign gene is sealed into the vector DNA by DNA ligas ...
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Nucleic acid analogue



Nucleic acid analogues are compounds which are analogous (structurally similar) to naturally occurring RNA and DNA, used in medicine and in molecular biology research.Nucleic acids are chains of nucleotides, which are composed of three parts: a phosphate backbone, a pucker-shaped pentose sugar, either ribose or deoxyribose, and one of four nucleobases.An analogue may have any of these altered. Typically the analogue nucleobases confer, among other things, different base pairing and base stacking properties. Examples include universal bases, which can pair with all four canonical bases, and phosphate-sugar backbone analogues such as PNA, which affect the properties of the chain (PNA can even form a triple helix).Nucleic acid analogues are also called Xeno Nucleic Acid and represent one of the main pillars of xenobiology, the design of new-to-nature forms of life based on alternative biochemistries.Artificial nucleic acids include peptide nucleic acid (PNA), Morpholino and locked nucleic acid (LNA), as well as glycol nucleic acid (GNA) and threose nucleic acid (TNA). Each of these is distinguished from naturally occurring DNA or RNA by changes to the backbone of the molecule.In May 2014, researchers announced that they had successfully introduced two new artificial nucleotides into bacterial DNA, and by including individual artificial nucleotides in the culture media, were able to passage the bacteria 24 times; they did not create mRNA or proteins able to use the artificial nucleotides. The artificial nucleotides featured 2 fused aromatic rings.
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