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video slide
video slide

... • Automation has allowed scientists to measure expression of thousands of genes at one time using DNA microarray assays • DNA microarray assays compare patterns of gene expression in different tissues, at different times, or under different conditions ...
1 - KSU Faculty Member websites
1 - KSU Faculty Member websites

... In which kind of cross would you expect to find a ratio of 9:3:3:1 among the F2 offspring? A. monohybrid cross B. dihybrid cross C. test cross D. none of the above Answer:B ...
ppt - Chair of Computational Biology
ppt - Chair of Computational Biology

... After the mRNA has been cleaved, around 250 adenosine residues are added to the free 3' end at the cleavage site. This reaction is catalyzed by polyadenylate polymerase. Just as in alternative splicing, there can be more than one polyadenylation variant of a mRNA. ...
Chemical Nature of the Amino Acids Table of a
Chemical Nature of the Amino Acids Table of a

Bioreg2017_Replication3_V4
Bioreg2017_Replication3_V4

IBC Risk Assessment Form_FINAL
IBC Risk Assessment Form_FINAL

... Experiments that Require NIH/OBA and IBC Approval Prior to Initiation (See page 16 of NIH Guidelines) Explanation: Experiments in this category cannot be initiated without submission of relevant information on the proposed experiment to NIH/OBA. The containment conditions for such experiments will b ...
3.1 Carbon`s Place in the Living World
3.1 Carbon`s Place in the Living World

... (b) Secondary structure Structural motifs, such as the corkscrew-like alpha helix, beta pleated sheets, and the less organized "random coils" are parts of many polypeptide chains, forming their secondary structure. (c) Tertiary structure These motifs may persist through a set of larger-scale turns t ...
广西医科大学理论课教案(1)
广西医科大学理论课教案(1)

... secondary, tertiary, quaternary structures, forces to make these structures stable, the rule to write the polypeptide chain 2.Know well important physicochemical properties of AA, three letters for AA, R group of AA, have familiar with some special example as Insulin, Hb, Mb structure characters 3. ...
Combinatorial  protein  design  by recombination in  vitro
Combinatorial protein design by recombination in vitro

... The evolutionary experiments described above all began with a single parental sequence. Mutations for recombination were produced by the point mutagenesis associated with DNA shuffling. Alternatively, they can be produced separately under controlled conditions, for example by PCR mutagenesis [18"]. ...
AP Biology Chapter 20 Biotechnology Guided Notes
AP Biology Chapter 20 Biotechnology Guided Notes

... Overview: The DNA Toolbox • ________________ of the genomes of more than 7,000 species was under way in 2010 • DNA sequencing has depended on advances in technology, starting with making ______________ • In _________________, nucleotide sequences from two different sources, often two species, are c ...
Chemical Synthesis of Oligonucleotides
Chemical Synthesis of Oligonucleotides

... remained labor intensive and inefficient throughout the 1970’s and into the early 1980’s. In 1983, a breakthrough was achieved in synthesis chemistry that made it possible to make longer oligonucleotides and to make them much more efficiently. The new synthesis process was based on the use of phosph ...
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LECT35 trans1

... Q: So, what’s the big deal? A: There are 20 amino acids; the code is degenerate There could be 4 “isoaccepting tRNAs” competing for one Q: I still don’t see a problem ...
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Chapter 2b Packet answers

CHM 105 - Jefferson State Community College
CHM 105 - Jefferson State Community College

... Understand the relationship of the hybridization of the carbon atom to the various types of carbon bonds. Apply the concepts of carbon bonding to distinguish and identify types of organic compounds. Apply the concepts of the versatility of carbon bonds in naming organic compounds using the IUPAC Sys ...
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Low cost flatbed scanner label-free biosensor - bu people

... light, and through demagnification optics, the image of the sample is projected onto the CCD sensor composed of red, green and blue elements. On the other hand, in CIS based scanners, the sample is sequentially illuminated with red, green and blue LEDs, and scattered light from the document is image ...
组蛋白甲基化
组蛋白甲基化

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Integration of Metabolism

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pdf
pdf

... most efficient if the 5' phosphate is removed (by alkaline phosphatase) prior to the kinase treatment. b. 3' end label: Klenow DNA polymerase plus [α 32P] dNTP. The labeled dNTP is chosen to be complementary to the first position past the primer. A restriction fragment with a 5' overhang is ideal fo ...
Key concepts for Essay #1
Key concepts for Essay #1

... DNA ligase seals Okazaki fragments together DNA polymerase proof-reads the new strands ...
MagNA Lyser Instrument
MagNA Lyser Instrument

... Automated tissue homogenization 1 x 106 ...
Chapter 2: Chemistry of Life
Chapter 2: Chemistry of Life

... 31. Describe each of the three subatomic particles with regard to charge, weight, and location in an atom. Ans: Protons are positively charged particles with a weight of one atomic mass unit that are located in the nucleus of an atom. Neutrons are uncharged particles with a weight of one atomic mas ...
SAMHD1 is a single-stranded nucleic acid
SAMHD1 is a single-stranded nucleic acid

... (14). The enzyme is evolutionarily related to a large superfamily of HD-domain proteins that contain a characteristic H...HD. . . D sequence motif required for divalent metal ion binding (15). Although the vast majority of the known HD family members have phosphohydrolase activities, recent studies ...
DNACatalyzed Lysine Side Chain Modification
DNACatalyzed Lysine Side Chain Modification

... achieved by using 5’-ImpDNA in the less preorganized architecture. From these results, we conclude that DNA can catalyze covalent modification of the nucleophilic Lys side chain, and a high degree of preorganization is dispensable when the electrophile is sufficiently reactive. In vitro selection[9] ...
Chapter 2: Chemistry of Life
Chapter 2: Chemistry of Life

... 31. Describe each of the three subatomic particles with regard to charge, weight, and location in an atom. Ans: Protons are positively charged particles with a weight of one atomic mass unit that are located in the nucleus of an atom. Neutrons are uncharged particles with a weight of one atomic mas ...
complete
complete

... Objectives: ...
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Nucleic acid analogue



Nucleic acid analogues are compounds which are analogous (structurally similar) to naturally occurring RNA and DNA, used in medicine and in molecular biology research.Nucleic acids are chains of nucleotides, which are composed of three parts: a phosphate backbone, a pucker-shaped pentose sugar, either ribose or deoxyribose, and one of four nucleobases.An analogue may have any of these altered. Typically the analogue nucleobases confer, among other things, different base pairing and base stacking properties. Examples include universal bases, which can pair with all four canonical bases, and phosphate-sugar backbone analogues such as PNA, which affect the properties of the chain (PNA can even form a triple helix).Nucleic acid analogues are also called Xeno Nucleic Acid and represent one of the main pillars of xenobiology, the design of new-to-nature forms of life based on alternative biochemistries.Artificial nucleic acids include peptide nucleic acid (PNA), Morpholino and locked nucleic acid (LNA), as well as glycol nucleic acid (GNA) and threose nucleic acid (TNA). Each of these is distinguished from naturally occurring DNA or RNA by changes to the backbone of the molecule.In May 2014, researchers announced that they had successfully introduced two new artificial nucleotides into bacterial DNA, and by including individual artificial nucleotides in the culture media, were able to passage the bacteria 24 times; they did not create mRNA or proteins able to use the artificial nucleotides. The artificial nucleotides featured 2 fused aromatic rings.
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