The energy-less red blood cell is lost: erythrocyte
... Hexokinase catalyzes the phosphorylation of glucose to G6P, using ATP as a phosphoryl donor (Figure 1). As the initial step of glycolysis, HK is one of the rate-limiting enzymes of this pathway. The activity of hexokinase is significantly higher in reticulocytes compared with mature red cells, in wh ...
... Hexokinase catalyzes the phosphorylation of glucose to G6P, using ATP as a phosphoryl donor (Figure 1). As the initial step of glycolysis, HK is one of the rate-limiting enzymes of this pathway. The activity of hexokinase is significantly higher in reticulocytes compared with mature red cells, in wh ...
protein research
... Takara’s pCold TF DNA Vector is a fusion cold shock expression vector that expresses Trigger Factor (TF) chaperone as a soluble tag. Trigger Factor is a prokaryotic ribosome-associated chaperone protein (48 kDa) which facilitates co-translational folding of newly expressed polypeptides. Because of i ...
... Takara’s pCold TF DNA Vector is a fusion cold shock expression vector that expresses Trigger Factor (TF) chaperone as a soluble tag. Trigger Factor is a prokaryotic ribosome-associated chaperone protein (48 kDa) which facilitates co-translational folding of newly expressed polypeptides. Because of i ...
Current Microbiology
... increases in the shoot and root weights at 10 days after sowing (Table 2). The shoot and root length of bacterially treated plants were not significantly different from those of control plants. However, the bacterization of maize seeds with the wild-type strain and mutants resulted in significant in ...
... increases in the shoot and root weights at 10 days after sowing (Table 2). The shoot and root length of bacterially treated plants were not significantly different from those of control plants. However, the bacterization of maize seeds with the wild-type strain and mutants resulted in significant in ...
Canine CD34: Cloning of the cDNA and Evaluation
... studies. The separation of progenitors is based on the expression of CD34, a marker preferentially expressed on progenitor cells. The dog modelhas been important forpreclinical transplant studies, because it has proven predictive for outcomes in human hematopoietic stem cell transplantation. To iden ...
... studies. The separation of progenitors is based on the expression of CD34, a marker preferentially expressed on progenitor cells. The dog modelhas been important forpreclinical transplant studies, because it has proven predictive for outcomes in human hematopoietic stem cell transplantation. To iden ...
Identification of a novel viral protein in infectious bursal disease
... purified IPNV. However, no correlation of these products with ORF A-2 has so far been shown, although it has been suggested that this protein is probably virusspecific. Comparison between the amino acid sequences of ORF A-2 proteins of different strains of IBDV and IPNV has indicated only limited am ...
... purified IPNV. However, no correlation of these products with ORF A-2 has so far been shown, although it has been suggested that this protein is probably virusspecific. Comparison between the amino acid sequences of ORF A-2 proteins of different strains of IBDV and IPNV has indicated only limited am ...
powerpoint
... Comparing Closely Related Species • Human and chimpanzee genomes differ by 1.2%, at single base-pairs, and by 2.7% because of insertions and deletions • Several genes are evolving faster in humans than chimpanzees • These include genes involved in defense against malaria and tuberculosis, regulatio ...
... Comparing Closely Related Species • Human and chimpanzee genomes differ by 1.2%, at single base-pairs, and by 2.7% because of insertions and deletions • Several genes are evolving faster in humans than chimpanzees • These include genes involved in defense against malaria and tuberculosis, regulatio ...
GENETICS TEST IV - Daytona State College
... • The TATA box is a core promoter element that binds the TATAbinding protein (TBP) of transcription factor TFIID and determines the start site of transcription ...
... • The TATA box is a core promoter element that binds the TATAbinding protein (TBP) of transcription factor TFIID and determines the start site of transcription ...
Membrane Protein Expression in Cell
... of MPs into lipid membranes (4). Depending on the selected CF protocol and reaction setup, incorporation efficiencies of supplied amino acids into the synthesised protein of higher than 10% can be obtained, resulting in the production of several milligrams of protein per 1 mL of reaction (5, 6). Fur ...
... of MPs into lipid membranes (4). Depending on the selected CF protocol and reaction setup, incorporation efficiencies of supplied amino acids into the synthesised protein of higher than 10% can be obtained, resulting in the production of several milligrams of protein per 1 mL of reaction (5, 6). Fur ...
Histidine Biosynthetic Pathway and Genes: Structure
... FIG. 1. Gene structure of the his operon of S. typhimurium and metabolic pathway of histidine biosynthesis. Top: the leader region (L) and structural genes are drawn to scale. Arrows indicate the relative positions of P1 (primary promoter) and P2 and P3 (internal promoters). The solid box represents ...
... FIG. 1. Gene structure of the his operon of S. typhimurium and metabolic pathway of histidine biosynthesis. Top: the leader region (L) and structural genes are drawn to scale. Arrows indicate the relative positions of P1 (primary promoter) and P2 and P3 (internal promoters). The solid box represents ...
What is MEROPS ?
... substrate specificity. The MEROPS database provides an alternative, hierarchical classification by domain structure (CLAN), sequence (FAMILY) and specificity (PEPTIDASE). There are no families of hypothetical proteins in MEROPS. A family contains peptidases of only one catalytic type. A clan, howeve ...
... substrate specificity. The MEROPS database provides an alternative, hierarchical classification by domain structure (CLAN), sequence (FAMILY) and specificity (PEPTIDASE). There are no families of hypothetical proteins in MEROPS. A family contains peptidases of only one catalytic type. A clan, howeve ...
Rapid increase of cytosolic content of acetyl-CoA
... Acetyl-CoA Carboxylase (ACC) catalyzes formation of malonyl-CoA from acetyl-CoA and CO2 which is the ratelimiting step in fatty acid biosynthesis (Wakil et al., 1983; Numa and Tanabe, 1984). Malonyl-CoA serves as a precursor of fatty acid biosynthesis and an intermediate of fatty acid elongation, bu ...
... Acetyl-CoA Carboxylase (ACC) catalyzes formation of malonyl-CoA from acetyl-CoA and CO2 which is the ratelimiting step in fatty acid biosynthesis (Wakil et al., 1983; Numa and Tanabe, 1984). Malonyl-CoA serves as a precursor of fatty acid biosynthesis and an intermediate of fatty acid elongation, bu ...
The Living World - Chapter 9 - McGraw Hill Higher Education
... Most eukaryotic genes exist in multiple copies Clusters of almost identical sequences called multigene families As few as three and as many as several hundred genes Transposable sequences or transposons are DNA sequences that can move about in the genome They are repeated thousands of times, scatter ...
... Most eukaryotic genes exist in multiple copies Clusters of almost identical sequences called multigene families As few as three and as many as several hundred genes Transposable sequences or transposons are DNA sequences that can move about in the genome They are repeated thousands of times, scatter ...
Document
... Most eukaryotic genes exist in multiple copies Clusters of almost identical sequences called multigene families As few as three and as many as several hundred genes Transposable sequences or transposons are DNA sequences that can move about in the genome They are repeated thousands of times, scatter ...
... Most eukaryotic genes exist in multiple copies Clusters of almost identical sequences called multigene families As few as three and as many as several hundred genes Transposable sequences or transposons are DNA sequences that can move about in the genome They are repeated thousands of times, scatter ...
Functional analysis of a type-2C protein phosphatase (AtPP2C52) in
... 4). AtPP2C52G99D and AtPP2C52DGH102-104ERN mutants failed to interact with UMP1 in Y2H (Fig. 4A). G105D mutation did not affect the Y2H interaction between AtPP2C52 and UMP1 (Fig. 4A). All of these mutations abolished the Y2H interaction between AtPP2C52 and RD21a (Fig. 4B). The interactions were ex ...
... 4). AtPP2C52G99D and AtPP2C52DGH102-104ERN mutants failed to interact with UMP1 in Y2H (Fig. 4A). G105D mutation did not affect the Y2H interaction between AtPP2C52 and UMP1 (Fig. 4A). All of these mutations abolished the Y2H interaction between AtPP2C52 and RD21a (Fig. 4B). The interactions were ex ...
Gene Expression
... Using quantitative real-time RT-PCR for gene expression profiling is a common, widely accepted technique. Currently, a user wishing to do such profiling has two choices: either develop the RT-PCR assays in house or order a pre-validated assay from a commercial supplier. Neither, however, is very tim ...
... Using quantitative real-time RT-PCR for gene expression profiling is a common, widely accepted technique. Currently, a user wishing to do such profiling has two choices: either develop the RT-PCR assays in house or order a pre-validated assay from a commercial supplier. Neither, however, is very tim ...
Bioinformatics in Brief This week: DB for structures Structure
... connectivity of the secondary structures (SSAP algorithm). Structures which have a SSAP score of 70 and where at least 60% of the larger protein matches the smaller protein are assigned to the same T level or fold family. Some fold families are very highly populated, they are currently subdivided us ...
... connectivity of the secondary structures (SSAP algorithm). Structures which have a SSAP score of 70 and where at least 60% of the larger protein matches the smaller protein are assigned to the same T level or fold family. Some fold families are very highly populated, they are currently subdivided us ...
Biochem10 - Amit Kessel Ph.D
... C. hormone-receptor complex in the nucleus D. free hormone bound to chromatin 26. Calmodulin A. is a calcium binding protein. B. mediates group translocation. C. is the regulatory subunit of cAMP-dependent protein kinase. D. is an integral membrane protein. E. can release calcium from internal store ...
... C. hormone-receptor complex in the nucleus D. free hormone bound to chromatin 26. Calmodulin A. is a calcium binding protein. B. mediates group translocation. C. is the regulatory subunit of cAMP-dependent protein kinase. D. is an integral membrane protein. E. can release calcium from internal store ...
The proton-linked monocarboxylate transporter (MCT) family
... [25,27,29,30]. MCT2 is not as widely distributed as MCT1, and there is also evidence for alternatively spliced mRNA species. Thus, for both rat and human MCT2, two separate groups have sequenced cDNA clones independently (see Table 1), and comparison of these sequences reveals the presence of two di ...
... [25,27,29,30]. MCT2 is not as widely distributed as MCT1, and there is also evidence for alternatively spliced mRNA species. Thus, for both rat and human MCT2, two separate groups have sequenced cDNA clones independently (see Table 1), and comparison of these sequences reveals the presence of two di ...
Supplementary Notes - Word file
... of the H3-triMeK4 mark on these actively transcribed genes can stabilize a repressive HDAC1 complex acutely at these genes, leading to deacetylation and transcriptional inactivation. By focusing HDAC1 repressor complexes on actively transcribed genes, recognition of H3-triMeK4 by ING2 may be importa ...
... of the H3-triMeK4 mark on these actively transcribed genes can stabilize a repressive HDAC1 complex acutely at these genes, leading to deacetylation and transcriptional inactivation. By focusing HDAC1 repressor complexes on actively transcribed genes, recognition of H3-triMeK4 by ING2 may be importa ...
Allantoin transport protein, PucI, from Bacillus subtilis
... primates), however, is non-functional, so uric acid is the end product of purine catabolism and this is excreted in the urine (Johnson et al., 2009). In most other mammals, allantoin is excreted in the urine, whilst in fish it is degraded further to ammonia before excretion. In bacteria, purines are ...
... primates), however, is non-functional, so uric acid is the end product of purine catabolism and this is excreted in the urine (Johnson et al., 2009). In most other mammals, allantoin is excreted in the urine, whilst in fish it is degraded further to ammonia before excretion. In bacteria, purines are ...
an introduction to alpha-fetoprotein and the growth inhibitory peptide
... Fig 8 Molecular configurations of human AFP (Left) and human albumin (Right) based on the predicted secondary structures. The amino acid residues participating in the formation of alpharespectively. helices, beta- sheets, beta-turns, and random coils are indicated by The loops formed by disulfide bo ...
... Fig 8 Molecular configurations of human AFP (Left) and human albumin (Right) based on the predicted secondary structures. The amino acid residues participating in the formation of alpharespectively. helices, beta- sheets, beta-turns, and random coils are indicated by The loops formed by disulfide bo ...
Lipids 44:
... proceeds via the x6 pathway [13, 14]. Unusual elongations and desaturations leading to the biosynthesis of VLCPUFA have been reported in the marine haptophyte Isochrysis galbana [15] and the fresh-water euglenophyte Euglena gracilis [16, 17]. In the alternative route, elongation of 18:2x-6 and 18:3x ...
... proceeds via the x6 pathway [13, 14]. Unusual elongations and desaturations leading to the biosynthesis of VLCPUFA have been reported in the marine haptophyte Isochrysis galbana [15] and the fresh-water euglenophyte Euglena gracilis [16, 17]. In the alternative route, elongation of 18:2x-6 and 18:3x ...
Characterization of Structural and Functional Properties of Human
... Recently, a model for the catalytic mechanism of 17HSD type 1 has been proposed by utilizing the threedimensional structure of the enzyme (21). In this model the side chains of Ser142, Tyr155, and Lys159 are involved in hydride transfer between the cofactor and 17-keto group of the substrate, and th ...
... Recently, a model for the catalytic mechanism of 17HSD type 1 has been proposed by utilizing the threedimensional structure of the enzyme (21). In this model the side chains of Ser142, Tyr155, and Lys159 are involved in hydride transfer between the cofactor and 17-keto group of the substrate, and th ...
Chapter 12
... • Mutations in the promoter are uninducible and cis-acting. • When mutant and wild-type subunits are present, a single lacI–d mutant subunit can inactivate a ...
... • Mutations in the promoter are uninducible and cis-acting. • When mutant and wild-type subunits are present, a single lacI–d mutant subunit can inactivate a ...
Base excision repair
... Transversion mutations replace a pyrimidine with a purine or vice versa. Both mutations can be permanently incorporated by DNA replication. ...
... Transversion mutations replace a pyrimidine with a purine or vice versa. Both mutations can be permanently incorporated by DNA replication. ...
Endogenous retrovirus
Endogenous retroviruses (ERVs) are endogenous viral elements in the genome that closely resemble and can be derived from retroviruses. They are abundant in the genomes of jawed vertebrates, and they comprise up to 5–8% of the human genome (lower estimates of ~1%). ERVs are a subclass of a type of gene called a transposon, which can be packaged and moved within the genome to serve a vital role in gene expression and in regulation. Researchers have suggested that retroviruses evolved from a type of transposable gene called a retrotransposon, which includes ERVs; these genes can mutate and instead of moving to another location in the genome they can become exogenous or pathogenic. This means that all ERVs may not have originated as an insertion by a retrovirus but that some may have been the source for the genetic information in the retroviruses they resemble.