Low-temperature anaerobic digestion is associated with differential
... McKeown et al. 2009). Although important insights have been gathered (e.g. Methanocorpusculum prevalence during AD operation at 15◦ C; McKeown et al. 2009), minimal information relating methanogenic presence to functional significance (metabolic pathways employed, physiological responses etc.) has b ...
... McKeown et al. 2009). Although important insights have been gathered (e.g. Methanocorpusculum prevalence during AD operation at 15◦ C; McKeown et al. 2009), minimal information relating methanogenic presence to functional significance (metabolic pathways employed, physiological responses etc.) has b ...
Multiple Manner Transposons in Flatworms and Hydras Are Related
... Figure 1. Alignment of the conceptual translations of mariner transposase PCR fragments from Qatworms and hydras with those of Insects, other Invertebrates, and humans. Sequences generated for this study are Indicated with an asterisk (only one sequence Is shown when two or more were very similar to ...
... Figure 1. Alignment of the conceptual translations of mariner transposase PCR fragments from Qatworms and hydras with those of Insects, other Invertebrates, and humans. Sequences generated for this study are Indicated with an asterisk (only one sequence Is shown when two or more were very similar to ...
Plasmid replication and control
... general rule that small plasmids tend to have a high copy number, sometimes over 100 copies per cell, whereas larger plasmids may be present in one or a few copies per cell. Certain classes of plasmids are capable of horizontal transmission from one bacterium to another. In most cases the recipient ...
... general rule that small plasmids tend to have a high copy number, sometimes over 100 copies per cell, whereas larger plasmids may be present in one or a few copies per cell. Certain classes of plasmids are capable of horizontal transmission from one bacterium to another. In most cases the recipient ...
Kinetic analysis of retroviral proteases by HELGA EIZERT
... Retroviruses are enveloped, positive-strand RNA viruses with a unique morphology and means of replication. These viruses are distinguished by their use of viral-encoded reverse transcriptase to copy virion genomic RNA into proviral DNA, which is integrated into the infected host cell genome. Thus, t ...
... Retroviruses are enveloped, positive-strand RNA viruses with a unique morphology and means of replication. These viruses are distinguished by their use of viral-encoded reverse transcriptase to copy virion genomic RNA into proviral DNA, which is integrated into the infected host cell genome. Thus, t ...
Biotechnology Timeline
... purified antibodies derived from only one clone of cells that recognize only one antigen. They later would share the 1984 Nobel Prize in Physiology or Medicine with Neils Jerne. ...
... purified antibodies derived from only one clone of cells that recognize only one antigen. They later would share the 1984 Nobel Prize in Physiology or Medicine with Neils Jerne. ...
Construction of an infectious cDNA clone for Omsk hemorrhagic
... in OHF-IC-ori (Fig 2). The OHF-IC-NS565-836, OHF-IC-NS565 and OHF-IC-NS5836 plasmids have the NS5 mutations, and OHF-IC-NS2A46 has the NS2A mutation. Recombinant viruses were recovered from these infectious cDNAs, and the growth curves were generated as described above. No significant differences we ...
... in OHF-IC-ori (Fig 2). The OHF-IC-NS565-836, OHF-IC-NS565 and OHF-IC-NS5836 plasmids have the NS5 mutations, and OHF-IC-NS2A46 has the NS2A mutation. Recombinant viruses were recovered from these infectious cDNAs, and the growth curves were generated as described above. No significant differences we ...
KNOX1 genes regulate lignin deposition and composition
... Gibberellin is a potent target for plant breeding and crop improvement since some agronomic traits such as fiber quality (Larkan et al., 2013), tree height, and growth rate (Yu et al., 2012) can be improved by increasing endogenous GA levels. Other traits such as changes to plant architecture respons ...
... Gibberellin is a potent target for plant breeding and crop improvement since some agronomic traits such as fiber quality (Larkan et al., 2013), tree height, and growth rate (Yu et al., 2012) can be improved by increasing endogenous GA levels. Other traits such as changes to plant architecture respons ...
Cloning and functional expression of invertebrate connexins from
... was amplified with 200 nM primers using the Eppendorf TripleMaster PCR system (Eppendorf AG, Hamburg, DE). After an initial denaturation at 94 °C for 5 min, 30 cycles of amplification with a MJ Research DNA engine Dyad (MJ Research, Boston, MA) were performed under the following conditions: 94 °C for ...
... was amplified with 200 nM primers using the Eppendorf TripleMaster PCR system (Eppendorf AG, Hamburg, DE). After an initial denaturation at 94 °C for 5 min, 30 cycles of amplification with a MJ Research DNA engine Dyad (MJ Research, Boston, MA) were performed under the following conditions: 94 °C for ...
ARF-Aux/IAA interactions through domain III/IV are not strictly
... as brassinolide in ChIP (chromatin immunoprecipitation) assay experiments. These results suggest that auxin levels might influence the targeting of ARFs to at least some AuxREs in promoters of auxin response genes. This might occur through direct effects on ARF binding to DNA target sites or throug ...
... as brassinolide in ChIP (chromatin immunoprecipitation) assay experiments. These results suggest that auxin levels might influence the targeting of ARFs to at least some AuxREs in promoters of auxin response genes. This might occur through direct effects on ARF binding to DNA target sites or throug ...
The Wiskott-Aldrich Syndrome and X-Linked
... infections, received a score of 2. Patients with persistent but manageable eczema, with recurrent infections, or with both received a score of 3. Those patients with persistent and difficult to control eczema associated with frequent potentially life-threatening infections were scored as 4. A score ...
... infections, received a score of 2. Patients with persistent but manageable eczema, with recurrent infections, or with both received a score of 3. Those patients with persistent and difficult to control eczema associated with frequent potentially life-threatening infections were scored as 4. A score ...
Genetics Test 3 Review Presentation
... opposite directions from a common origin. • Bacteria DNA replication begins at the origin of replication and is bidirectional rather than unidirectional. ...
... opposite directions from a common origin. • Bacteria DNA replication begins at the origin of replication and is bidirectional rather than unidirectional. ...
Autotaxin–Lysophosphatidic Acid Axis Acts Downstream of
... chylomicrons, low-density lipoproteins (LDLs), and verylow–density lipoproteins (VLDL), modulate angiogenesis by regulating the expression of the endothelial decoy receptor VEGFR1.10 Zebrafish stalactite (stl) mutants, which carry a mutation in the microsomal triglyceride transfer protein (mtp) gene ...
... chylomicrons, low-density lipoproteins (LDLs), and verylow–density lipoproteins (VLDL), modulate angiogenesis by regulating the expression of the endothelial decoy receptor VEGFR1.10 Zebrafish stalactite (stl) mutants, which carry a mutation in the microsomal triglyceride transfer protein (mtp) gene ...
Mutagenesis identifies the critical amino acid residues of human
... Conclusion: Based on the mutational analysis and homology modeling, we proposed that human EndoG shared a similar catalytic mechanism with nuclease A from Anabaena. ...
... Conclusion: Based on the mutational analysis and homology modeling, we proposed that human EndoG shared a similar catalytic mechanism with nuclease A from Anabaena. ...
Identification of TIpC, a novel 62 kDa MCP
... of high conservation was found to be localized within the C-terminal domain (Fig. 4), with no detectable homology in the N-terminal region. An amino acid alignment between TlpC and a representative E. coli transducer Tar showed 30.0 o/' identity in a 297 amino acid overlap (Fig. 4). The similarity b ...
... of high conservation was found to be localized within the C-terminal domain (Fig. 4), with no detectable homology in the N-terminal region. An amino acid alignment between TlpC and a representative E. coli transducer Tar showed 30.0 o/' identity in a 297 amino acid overlap (Fig. 4). The similarity b ...
Regulation of nitrogen metabolism in gram
... nitrogen excess, GlnR functions as a repressor of the glnRA operon [5]. In contrast to GlnR, TnrA may repress as well as activate transcription. Under conditions of an excess of glutamine and glutamate in the cell, TnrA binds to glutamine synthase, forming an inactive complex. As a result, TnrA cann ...
... nitrogen excess, GlnR functions as a repressor of the glnRA operon [5]. In contrast to GlnR, TnrA may repress as well as activate transcription. Under conditions of an excess of glutamine and glutamate in the cell, TnrA binds to glutamine synthase, forming an inactive complex. As a result, TnrA cann ...
Pax8, a murine paired box gene expressed in the developing
... constitute a segmented structure. Hence, the expression of Pax2 in this tissue supports a possible role for this gene in the segmentation of the mouse embryo. Several paired box sequences have been detected in the mouse genome by hybridization (Dressier et al. 1988). As a first step to understanding ...
... constitute a segmented structure. Hence, the expression of Pax2 in this tissue supports a possible role for this gene in the segmentation of the mouse embryo. Several paired box sequences have been detected in the mouse genome by hybridization (Dressier et al. 1988). As a first step to understanding ...
The energy-less red blood cell is lost – erythrocyte
... functioning, such as brain, muscle, and erythrocytes. HK-I displays unique regulatory properties in its sensitivity to inhibition by physiological levels of the product G6P and relief of this inhibition by inorganic phosphate.14,15 The determination of the structures of the human (PDB entry 1DGK) an ...
... functioning, such as brain, muscle, and erythrocytes. HK-I displays unique regulatory properties in its sensitivity to inhibition by physiological levels of the product G6P and relief of this inhibition by inorganic phosphate.14,15 The determination of the structures of the human (PDB entry 1DGK) an ...
NOVEL SPERM-SPECIFIC GLYCOLYTIC ISOZYMES GENERATED
... the glycolytic pathway. Genomic and proteomic analyses identified three aldolase A (Aldoa) transcripts generated by retrotransposition and alternative splicing: Aldoa_v2, Aldoart1, and Aldoart2. Unique nucleotide sequence in Aldoart1 provided evidence for an alternatively spliced exon in Aldoa_v2. E ...
... the glycolytic pathway. Genomic and proteomic analyses identified three aldolase A (Aldoa) transcripts generated by retrotransposition and alternative splicing: Aldoa_v2, Aldoart1, and Aldoart2. Unique nucleotide sequence in Aldoart1 provided evidence for an alternatively spliced exon in Aldoa_v2. E ...
Using CRISPR for genetic alteration_Joffrey Maine
... used. This additional modification will prevent the system to “re-process/recut” the allele repair through Homology Directed Repair (HDR) with the donor oligo (= expected allele). ...
... used. This additional modification will prevent the system to “re-process/recut” the allele repair through Homology Directed Repair (HDR) with the donor oligo (= expected allele). ...
Journal of Biotechnology 84:
... formation of Organo-P compounds with the organic acids, which are further used as nutrient source by the bacteria. These evidences also support the idea that P concentration is not a reliable parameter for assessing phosphate solubilization capability of a microbial strain for comparative studies. I ...
... formation of Organo-P compounds with the organic acids, which are further used as nutrient source by the bacteria. These evidences also support the idea that P concentration is not a reliable parameter for assessing phosphate solubilization capability of a microbial strain for comparative studies. I ...
Deletion of the gene encoding the reductase component of 3
... resulting in the formation of 4-cholesten-3-one. However, recent studies on some Mycobacterium strains suggested that 3-hydroxysteroid dehydrogenase, rather than cholesterol oxidase, is responsible for the first step oxidation [5,6]. The degradation continues with side chain cleavage, taking place v ...
... resulting in the formation of 4-cholesten-3-one. However, recent studies on some Mycobacterium strains suggested that 3-hydroxysteroid dehydrogenase, rather than cholesterol oxidase, is responsible for the first step oxidation [5,6]. The degradation continues with side chain cleavage, taking place v ...
Identification of Two Mammalian Reductases
... not been assigned to Ssc2, although two of its substrates are arachidonic (20:4) and eicosapentaenoic (20:5) acids (1). ELOVL4 (elongation of very long chain fatty acids-like 4) was identified by linkage and haplotype analysis in families with two forms of autosomal dominant macular dystrophy and is ...
... not been assigned to Ssc2, although two of its substrates are arachidonic (20:4) and eicosapentaenoic (20:5) acids (1). ELOVL4 (elongation of very long chain fatty acids-like 4) was identified by linkage and haplotype analysis in families with two forms of autosomal dominant macular dystrophy and is ...
Molecular cloning and nucleotide sequence of another variant of the
... H.I.8 is a member of the SLTII family. Since SLTIIva shares with SLTIIv the property of having low cytotoxicity to HeLa cells compared with Vero cells, it is appropriateto consider both toxins as variants of SLTII. SLTIIva M e r s from SLTIIv in that it is more heat-stable. Further, SLTIIv-producing ...
... H.I.8 is a member of the SLTII family. Since SLTIIva shares with SLTIIv the property of having low cytotoxicity to HeLa cells compared with Vero cells, it is appropriateto consider both toxins as variants of SLTII. SLTIIva M e r s from SLTIIv in that it is more heat-stable. Further, SLTIIv-producing ...
(THCA) synthase gene in
... The cannabinoid content of 13 different strains of cannabis plant (Cannabis sativa L.) was analyzed. Six strains fell into the ‘‘drug-type’’ class, with high D-9-tetrahydrocannabinolic acid (THCA) content, and seven strains into the ‘‘fiber-type’’ class, with low THCA using HPLC analysis. Genomic DN ...
... The cannabinoid content of 13 different strains of cannabis plant (Cannabis sativa L.) was analyzed. Six strains fell into the ‘‘drug-type’’ class, with high D-9-tetrahydrocannabinolic acid (THCA) content, and seven strains into the ‘‘fiber-type’’ class, with low THCA using HPLC analysis. Genomic DN ...
Lesson Overview - Dr. Thornton`s Courses
... produced from a single cell The technique of cloning uses a single cell from an adult organism to grow an entirely new individual that is genetically identical to the organism from which the cell was taken. Clones of animals were first produced in 1952 using amphibian ...
... produced from a single cell The technique of cloning uses a single cell from an adult organism to grow an entirely new individual that is genetically identical to the organism from which the cell was taken. Clones of animals were first produced in 1952 using amphibian ...
Endogenous retrovirus
Endogenous retroviruses (ERVs) are endogenous viral elements in the genome that closely resemble and can be derived from retroviruses. They are abundant in the genomes of jawed vertebrates, and they comprise up to 5–8% of the human genome (lower estimates of ~1%). ERVs are a subclass of a type of gene called a transposon, which can be packaged and moved within the genome to serve a vital role in gene expression and in regulation. Researchers have suggested that retroviruses evolved from a type of transposable gene called a retrotransposon, which includes ERVs; these genes can mutate and instead of moving to another location in the genome they can become exogenous or pathogenic. This means that all ERVs may not have originated as an insertion by a retrovirus but that some may have been the source for the genetic information in the retroviruses they resemble.