Dynamics of the cellular metabolome during human cytomegalovirus infection.
... Viral replication requires energy and macromolecular precursors derived from the metabolic network of the host cell. Despite this reliance, the effect of viral infection on host cell metabolic composition remains poorly understood. Here we applied liquid chromatography-tandem mass spectrometry to me ...
... Viral replication requires energy and macromolecular precursors derived from the metabolic network of the host cell. Despite this reliance, the effect of viral infection on host cell metabolic composition remains poorly understood. Here we applied liquid chromatography-tandem mass spectrometry to me ...
Cloning and characterization of a phosphopantetheinyl transferase
... attachment of the P-pant group to a conserved serine residue of the carrier proteins. This posttranslational modi¢cation is catalyzed by a superfamily of enzymes known as phosphopantetheinyl transferases (PPTases) that derive the P-pant group from coenzyme A (CoA) [9,10]. In organisms containing mul ...
... attachment of the P-pant group to a conserved serine residue of the carrier proteins. This posttranslational modi¢cation is catalyzed by a superfamily of enzymes known as phosphopantetheinyl transferases (PPTases) that derive the P-pant group from coenzyme A (CoA) [9,10]. In organisms containing mul ...
THE EVOLUTION OF ACETYL-CoA SYNTHASE 1
... two types of ACS enzymes, depending on whether cells are grown autotrophically or heterotrophically (Schauder et al., 1989). ACS enzymes in sulfate-reducing bacteria are not well characterized. The anaerobic Ni-requiring homoacetogenic bacterium C. thermoaceticum catabolizes glucose, forming three a ...
... two types of ACS enzymes, depending on whether cells are grown autotrophically or heterotrophically (Schauder et al., 1989). ACS enzymes in sulfate-reducing bacteria are not well characterized. The anaerobic Ni-requiring homoacetogenic bacterium C. thermoaceticum catabolizes glucose, forming three a ...
GRE BIOCHEMISTRY TEST PRACTICE BOOK
... expect to have subscores equal to about one-tenth of his or her total score. Thus, if you have a total score of 600, and your undergraduate program placed equal emphasis on the three areas of biochemistry, cell and molecular biology represented by the subscores, you would expect to have a scaled sco ...
... expect to have subscores equal to about one-tenth of his or her total score. Thus, if you have a total score of 600, and your undergraduate program placed equal emphasis on the three areas of biochemistry, cell and molecular biology represented by the subscores, you would expect to have a scaled sco ...
Document
... and DNA Replication Gene expression and DNA replication are compared in Table I-I-I. Transcription, the first stage in gene expression, involves transfer of information found in a double-stranded DNA molecule to the base sequence of a single-stranded RNA molecule. If the RNA molecule is a messenger ...
... and DNA Replication Gene expression and DNA replication are compared in Table I-I-I. Transcription, the first stage in gene expression, involves transfer of information found in a double-stranded DNA molecule to the base sequence of a single-stranded RNA molecule. If the RNA molecule is a messenger ...
BI280 Principles of Microbiology w-Lab
... o. Contrast and give examples of simple and differential stains. p. Recall and give examples of staining terminology associated with differential stains. q. Arrange bacterial flagella according to their position and number. r. Name the cellular antigen designation for the common bacterial anatomical ...
... o. Contrast and give examples of simple and differential stains. p. Recall and give examples of staining terminology associated with differential stains. q. Arrange bacterial flagella according to their position and number. r. Name the cellular antigen designation for the common bacterial anatomical ...
Absence of translationally selected synonymous codon usage
... protein-encoding sequences, which were extracted using WWW-Query at the PBIL (Po# le Bio-Informatique Lyonnais) World Wide Web site (http :}}pbil.univ-lyon1.fr}). A number of genes were excluded from our main analyses. Twenty-six genes occur in the 40 kb cag pathogenicity island, which has a differe ...
... protein-encoding sequences, which were extracted using WWW-Query at the PBIL (Po# le Bio-Informatique Lyonnais) World Wide Web site (http :}}pbil.univ-lyon1.fr}). A number of genes were excluded from our main analyses. Twenty-six genes occur in the 40 kb cag pathogenicity island, which has a differe ...
A P element-homologous sequence in the house fly, Musca domestica
... inverted, subterminal, repeats. They have four open reading frames, all of which are required to encode a transposase enzyme. Defective P elements are generally smaller and variable in size and are derived from complete elements by internal deletions. The induction of these deletions is considered t ...
... inverted, subterminal, repeats. They have four open reading frames, all of which are required to encode a transposase enzyme. Defective P elements are generally smaller and variable in size and are derived from complete elements by internal deletions. The induction of these deletions is considered t ...
paper - Lirmm
... approach: BLASTN on DNA and mRNA, or BLASTX on mRNA and proteins, applicable only when the DNA sequences are sufficiently similar. BLASTX programs, although capable of insightful results thanks to the six frame translations, have the limitation of not being able to transparently manage frameshifts t ...
... approach: BLASTN on DNA and mRNA, or BLASTX on mRNA and proteins, applicable only when the DNA sequences are sufficiently similar. BLASTX programs, although capable of insightful results thanks to the six frame translations, have the limitation of not being able to transparently manage frameshifts t ...
MycoplasMa Quality control
... Importance of routine testing Because mycoplasma can affect the phenotypic and functional characteristics of cells in vitro, the implementation of early detection methods that are rapid and sensitive is an important step towards preventing the deleterious and costly effects of mycoplasma on research ...
... Importance of routine testing Because mycoplasma can affect the phenotypic and functional characteristics of cells in vitro, the implementation of early detection methods that are rapid and sensitive is an important step towards preventing the deleterious and costly effects of mycoplasma on research ...
Caloranaerobacter ferrireducens sp. nov., an anaerobic
... acid profiles of strain DY22619T and Caloranaerobacter azorensis MV1087T are given in Table S1. The G+C content of the chromosomal DNA was determined according to the methods described using reverse-phase HPLC (Mesbah & Whitman, 1989). The DNA G+C content of the novel isolate DY22619T was 29.0 mol%, ...
... acid profiles of strain DY22619T and Caloranaerobacter azorensis MV1087T are given in Table S1. The G+C content of the chromosomal DNA was determined according to the methods described using reverse-phase HPLC (Mesbah & Whitman, 1989). The DNA G+C content of the novel isolate DY22619T was 29.0 mol%, ...
"Allosteric Activation of Kinases: Design and Application of RapR
... RapR approach to constitutively active kinases. This insures that kinase activity is strictly under the control of the experimentalist, and not subject to regulation by endogenous signaling pathways. In some studies, it may be valuable to activate wild-type, regulated kinase. For the actual design o ...
... RapR approach to constitutively active kinases. This insures that kinase activity is strictly under the control of the experimentalist, and not subject to regulation by endogenous signaling pathways. In some studies, it may be valuable to activate wild-type, regulated kinase. For the actual design o ...
Identification, Expression and Characterization of Archaeal
... 6.1.3. P. pastoris Culture............................................................................................. 78 6.1.3.1. Preparation of P. pastoris competent cells ................................................. 79 6.1.3.2. Transformation and expression Chop-1SS in P. pastoris (GS115).. ...
... 6.1.3. P. pastoris Culture............................................................................................. 78 6.1.3.1. Preparation of P. pastoris competent cells ................................................. 79 6.1.3.2. Transformation and expression Chop-1SS in P. pastoris (GS115).. ...
... generated from the polyk mRNA of this tumor (Fig. 3E). These observations indicated that the two classes of clones corresponded to the transcripts coding for the most basic and the most acidic 29-kDa proteins. For the first group of hepatocellular carcinoma cDNA clones, sequence information was obta ...
Ces locus embedded proteins control the non
... was assessed. The hydrolase CesH turned out to be a part of the complex regulatory network controlling cereulide synthesis on a transcriptional level, while the ABC transporter CesCD was found to be essential for post-translational control of cereulide synthesis. Using a gene inactivation approach, ...
... was assessed. The hydrolase CesH turned out to be a part of the complex regulatory network controlling cereulide synthesis on a transcriptional level, while the ABC transporter CesCD was found to be essential for post-translational control of cereulide synthesis. Using a gene inactivation approach, ...
$doc.title
... The rapid spread of human immunodeficiency virus type 1 (HIV-‐1) has been accompanied by continuous extensive viral genetic diversification. Little is known about how virus diversification is infl ...
... The rapid spread of human immunodeficiency virus type 1 (HIV-‐1) has been accompanied by continuous extensive viral genetic diversification. Little is known about how virus diversification is infl ...
XSL Formatter - H:\XML
... large sequencing project devoted to a particular genome. These entries have a streamlined submission process and undergo minimal processing before being loaded to GenBank. ESTs are generally short (<1 kb), single-pass cDNA sequences from a particular tissue and/or developmental stage. However, they ...
... large sequencing project devoted to a particular genome. These entries have a streamlined submission process and undergo minimal processing before being loaded to GenBank. ESTs are generally short (<1 kb), single-pass cDNA sequences from a particular tissue and/or developmental stage. However, they ...
Heptad repeat sequences are located adjacent to hydrophobic
... paramyxovirus fusion and coronavirus peplomer glycoproteins are reasonably hydrophilic despite the regular pattern of hydrophobic amino acids in positions a and d, consistent with the formation of an extended structure (Cohen & Parry, 1986). The heptad regions of some retroviruses, for example human ...
... paramyxovirus fusion and coronavirus peplomer glycoproteins are reasonably hydrophilic despite the regular pattern of hydrophobic amino acids in positions a and d, consistent with the formation of an extended structure (Cohen & Parry, 1986). The heptad regions of some retroviruses, for example human ...
Heptad repeat sequences are located adjacent to hydrophobic
... paramyxovirus fusion and coronavirus peplomer glycoproteins are reasonably hydrophilic despite the regular pattern of hydrophobic amino acids in positions a and d, consistent with the formation of an extended structure (Cohen & Parry, 1986). The heptad regions of some retroviruses, for example human ...
... paramyxovirus fusion and coronavirus peplomer glycoproteins are reasonably hydrophilic despite the regular pattern of hydrophobic amino acids in positions a and d, consistent with the formation of an extended structure (Cohen & Parry, 1986). The heptad regions of some retroviruses, for example human ...
Coevolution of an aminoacyl-tRNA synthetase with its tRNA substrates
... (from E. coli to D. radiodurans and Corynebacterium) of the genes encoding these precisely truncated GluRS fragments suggests that these proteins may have a yet unknown function. Nonetheless, GluX was not analyzed further. Class I lysyl-tRNA synthetase (LysRS) is structurally very related (27) and d ...
... (from E. coli to D. radiodurans and Corynebacterium) of the genes encoding these precisely truncated GluRS fragments suggests that these proteins may have a yet unknown function. Nonetheless, GluX was not analyzed further. Class I lysyl-tRNA synthetase (LysRS) is structurally very related (27) and d ...
FC Chimeric Fusion Protein Purified Human
... Fas (APO-1, CD95) is a 45 kDa cell surface protein that mediates apoptosis when cross-linked with agonistic anti-Fas antibodies or Fas ligand (FasL). Fas belongs to the TNF (tumor necrosis factor)/NGF (nerve growth factor) receptor family, and is expressed in various tissues and cells including the ...
... Fas (APO-1, CD95) is a 45 kDa cell surface protein that mediates apoptosis when cross-linked with agonistic anti-Fas antibodies or Fas ligand (FasL). Fas belongs to the TNF (tumor necrosis factor)/NGF (nerve growth factor) receptor family, and is expressed in various tissues and cells including the ...
E. coli
... Institute of Theoretical Biophysics (Russian Academy of Sciences, Puschino, Russia), led by Prof. E. Selkov. • It represents a key resource for developing detailed metabolic reconstructions for newly sequenced genomes far more rapidly than researchers even a few years ago would have thought possible ...
... Institute of Theoretical Biophysics (Russian Academy of Sciences, Puschino, Russia), led by Prof. E. Selkov. • It represents a key resource for developing detailed metabolic reconstructions for newly sequenced genomes far more rapidly than researchers even a few years ago would have thought possible ...
Gene Section NOTCH2 (Notch homolog 2 (Drosophila)) Atlas of Genetics and Cytogenetics
... lung, intestine, skeletal muscle and liver (hepatic cells and bile duct cells), ameloblasts. ...
... lung, intestine, skeletal muscle and liver (hepatic cells and bile duct cells), ameloblasts. ...
Systemic Organ Wasting Induced by Localized Expression of the
... Yorkie, the Yap1 oncogene ortholog, in intestinal stem cells leads to wasting of the ovary, fat body, and muscle. These organ-wasting phenotypes are associated with a reduction in systemic insulin/ IGF signaling due to increased expression of the secreted insulin/IGF antagonist ImpL2 from the overpr ...
... Yorkie, the Yap1 oncogene ortholog, in intestinal stem cells leads to wasting of the ovary, fat body, and muscle. These organ-wasting phenotypes are associated with a reduction in systemic insulin/ IGF signaling due to increased expression of the secreted insulin/IGF antagonist ImpL2 from the overpr ...
Biology: semester one: course outline
... photosynthesis. In general terms, explain the role of redox reactions in photosynthesis. Describe the relationship between an action spectrum and an absorption spectrum. List the components of a photosystem and explain the function of each component. Trace the movement of electrons in linear electro ...
... photosynthesis. In general terms, explain the role of redox reactions in photosynthesis. Describe the relationship between an action spectrum and an absorption spectrum. List the components of a photosystem and explain the function of each component. Trace the movement of electrons in linear electro ...
Endogenous retrovirus
Endogenous retroviruses (ERVs) are endogenous viral elements in the genome that closely resemble and can be derived from retroviruses. They are abundant in the genomes of jawed vertebrates, and they comprise up to 5–8% of the human genome (lower estimates of ~1%). ERVs are a subclass of a type of gene called a transposon, which can be packaged and moved within the genome to serve a vital role in gene expression and in regulation. Researchers have suggested that retroviruses evolved from a type of transposable gene called a retrotransposon, which includes ERVs; these genes can mutate and instead of moving to another location in the genome they can become exogenous or pathogenic. This means that all ERVs may not have originated as an insertion by a retrovirus but that some may have been the source for the genetic information in the retroviruses they resemble.