Biotechnology for Biofuels
... markers, and a large number of colonies often need to be screened to identify a mutant [23]. The intron-based gene knockout system used to construct these mutations was especially advantageous for the manipulation of these obligately anaerobic clostridia. The lack of a selectable marker in the intro ...
... markers, and a large number of colonies often need to be screened to identify a mutant [23]. The intron-based gene knockout system used to construct these mutations was especially advantageous for the manipulation of these obligately anaerobic clostridia. The lack of a selectable marker in the intro ...
PhytoREF: a reference database of the plastidial 16S
... of photosynthetic eukaryotes in the natural environment using classical microscopy-based techniques. For most taxa, taxonomic identification is greatly hindered by their minute size (as small as 0.8 µm for the prasinophyte Ostreococcus; Courties et al. 1994; Vaulot et al. 2008), lack of distinctive ...
... of photosynthetic eukaryotes in the natural environment using classical microscopy-based techniques. For most taxa, taxonomic identification is greatly hindered by their minute size (as small as 0.8 µm for the prasinophyte Ostreococcus; Courties et al. 1994; Vaulot et al. 2008), lack of distinctive ...
Bovine Angiotensin Converting Enzyme cDNA Cloning and
... ,ul) were removed at 15 and 30 minutes and acidified to prevent further reaction. The aliquots were then extracted with Ventrex scintillation cocktail No. 2, and the hydrolyzed ['H]benzoyl-phenylalanine was measured by liquid scintillation spectrometry. For determination of specific ACE activity, pa ...
... ,ul) were removed at 15 and 30 minutes and acidified to prevent further reaction. The aliquots were then extracted with Ventrex scintillation cocktail No. 2, and the hydrolyzed ['H]benzoyl-phenylalanine was measured by liquid scintillation spectrometry. For determination of specific ACE activity, pa ...
ARTÍCULOS
... characterize the interaction between H-NS and Hha. We show that the presence of DNA in preparations of copurified His-Hha and H-NS is not directly implicated in the interaction between the proteins. The precise molecular mass of the H-NS protein retained by Hha, obtained by mass spectrometry analysis ...
... characterize the interaction between H-NS and Hha. We show that the presence of DNA in preparations of copurified His-Hha and H-NS is not directly implicated in the interaction between the proteins. The precise molecular mass of the H-NS protein retained by Hha, obtained by mass spectrometry analysis ...
Fatty acid transport proteins: a current view of a
... utilizing organs. The differential distribution of glucose among organs is facilitated by tissue-specific expression of a family of different glucose transporters, in addition to different abilities to respond to insulin changes34. The mechanisms by which the flux of LCFAs into organs is regulated a ...
... utilizing organs. The differential distribution of glucose among organs is facilitated by tissue-specific expression of a family of different glucose transporters, in addition to different abilities to respond to insulin changes34. The mechanisms by which the flux of LCFAs into organs is regulated a ...
A novel species of thermoacidophilic archaeon, Sulfolobus
... DNA-DNA hybridization. DNA was isolated according to the method of Silhavy et al. (1984), sonicated and denatured by heating in a 95 "C dry bath. DNA was labelled with [cx32P]dCTP by using the Rediprime DNA labelling system (Amersham) and was then mixed with unlabelled DNA (a total of 200ng DNA) for ...
... DNA-DNA hybridization. DNA was isolated according to the method of Silhavy et al. (1984), sonicated and denatured by heating in a 95 "C dry bath. DNA was labelled with [cx32P]dCTP by using the Rediprime DNA labelling system (Amersham) and was then mixed with unlabelled DNA (a total of 200ng DNA) for ...
Summary and conclusions - Food Standards Australia New Zealand
... products have a range of food and feed as well as industrial uses and have a long history of safe use for both humans and livestock. Oil, in one form or another, accounts for the major food use of soybean and is incorporated in salad and cooking oil, bakery shortening, and frying fat as well as proc ...
... products have a range of food and feed as well as industrial uses and have a long history of safe use for both humans and livestock. Oil, in one form or another, accounts for the major food use of soybean and is incorporated in salad and cooking oil, bakery shortening, and frying fat as well as proc ...
Odyssey of Agrobacterium T-DNA.
... was suggested to function in T-DNA integration, as it is covalently attached to the 5¢ end of T-DNA, pilots T-DNA to the plant nucleus and probably stays attached to T-DNA up to the integration step. Two hypotheses of possible function of VirD2 in T-DNA integration have been proposed: (i) VirD2 acts ...
... was suggested to function in T-DNA integration, as it is covalently attached to the 5¢ end of T-DNA, pilots T-DNA to the plant nucleus and probably stays attached to T-DNA up to the integration step. Two hypotheses of possible function of VirD2 in T-DNA integration have been proposed: (i) VirD2 acts ...
Understanding a Millennium of Hemoglobin Evolution: Correlating
... evolutionary pressure. It also compares the behavior of the isolated chains to similar chains within the intact hemoglobin complex. The hemoglobin (Hb) molecule, being one of the most well-studied protein systems, has a long evolutionary history. Its discovery in virtually all kingdoms of organisms ...
... evolutionary pressure. It also compares the behavior of the isolated chains to similar chains within the intact hemoglobin complex. The hemoglobin (Hb) molecule, being one of the most well-studied protein systems, has a long evolutionary history. Its discovery in virtually all kingdoms of organisms ...
Functional characterization of dosage‐dependent lethal mutation of
... which lacks the UBI4 gene, grows normally in the absence of any kind of stress. Previous studies from our laboratory established that over-expression of UbEP42 in this strain caused cell lysis even under normal conditions. SUB62, being a wild-type strain for UBI4, by contrast remained unaffected by ...
... which lacks the UBI4 gene, grows normally in the absence of any kind of stress. Previous studies from our laboratory established that over-expression of UbEP42 in this strain caused cell lysis even under normal conditions. SUB62, being a wild-type strain for UBI4, by contrast remained unaffected by ...
Amino Acid Sequence of a Platelet-Binding Human Anti
... et al.’2 A 1% solution of NH4I-1C03 (pH 8.0) was added to Fv (100 Lg) to yield a final protein concentration of 5 mg/mL. ...
... et al.’2 A 1% solution of NH4I-1C03 (pH 8.0) was added to Fv (100 Lg) to yield a final protein concentration of 5 mg/mL. ...
HIV protease cleaves poly(A)
... SV-PR-expressing cells when SQ was present (Figure 2A). Cell extracts were analysed further to determine the cleavage of eIF4GI and eIF4GII (Figures 2B and 2C). Previous findings revealed the presence of two forms of eIF4G of 220 and 150 kDa in BHK21 cells [27,28]. These different eIF4G forms may be ...
... SV-PR-expressing cells when SQ was present (Figure 2A). Cell extracts were analysed further to determine the cleavage of eIF4GI and eIF4GII (Figures 2B and 2C). Previous findings revealed the presence of two forms of eIF4G of 220 and 150 kDa in BHK21 cells [27,28]. These different eIF4G forms may be ...
III BSC BT - 609 A -core- Animal Biotechnology
... D. None of the above ANSWER: A 67. Connective tissue around seminiferous tubules possess endocrine cells called ________ . A. leydig cells. B. sertoli cells. C. sertoli cells. D. both a and b. ANSWER: A 68. Conversion of spermatids into sperms is ___________ . A. spermiogenesis. B. spermatogenesis. ...
... D. None of the above ANSWER: A 67. Connective tissue around seminiferous tubules possess endocrine cells called ________ . A. leydig cells. B. sertoli cells. C. sertoli cells. D. both a and b. ANSWER: A 68. Conversion of spermatids into sperms is ___________ . A. spermiogenesis. B. spermatogenesis. ...
Restriction fragment differential display of pediocin
... was a dideoxynucleotide (the so-called extension protection group). The incubation was carried out at 37 mC and not the standard 16 mC to maintain the TaqI endonuclease activity during the ligation. This prevented religation of cDNA TaqI fragments, but not adaptor ligation, since the latter did not ...
... was a dideoxynucleotide (the so-called extension protection group). The incubation was carried out at 37 mC and not the standard 16 mC to maintain the TaqI endonuclease activity during the ligation. This prevented religation of cDNA TaqI fragments, but not adaptor ligation, since the latter did not ...
Protein Kinases - School of Medicine
... • A search of the human genome for tyrosine kinase coding elements identified several novel genes and enabled the creation of a nonredundant catalog of tyrosine kinase genes • Ninety unique kinase genes can be identified in the human genome, along with five pseudogenes – Of the 90 tyrosine kinases, ...
... • A search of the human genome for tyrosine kinase coding elements identified several novel genes and enabled the creation of a nonredundant catalog of tyrosine kinase genes • Ninety unique kinase genes can be identified in the human genome, along with five pseudogenes – Of the 90 tyrosine kinases, ...
Sequence-Specific Deoxyribonucleic Acid (DNA) Recognition by
... is that many genes appear to be regulated in collaboration with proximally bound, structurally diverse transcription factors including cAMP response element binding protein (CREB), CCAAT-enhancer binding protein (C/EBP-!), pituitary homeobox (Pitx), GATA-4, early growth response protein 1 (EGR-1), ! ...
... is that many genes appear to be regulated in collaboration with proximally bound, structurally diverse transcription factors including cAMP response element binding protein (CREB), CCAAT-enhancer binding protein (C/EBP-!), pituitary homeobox (Pitx), GATA-4, early growth response protein 1 (EGR-1), ! ...
Proteomics Analysis of Immunoprecipitated Proteins Associated with
... to have higher specific activity than the wild-type protein, although the level of phosphorylation of the substrate ERK was similar. The region between residues 425 and 467 (termed a ‘kinase repression domain’) was thought to be largely responsible for the difference in activity. A putative PEST seq ...
... to have higher specific activity than the wild-type protein, although the level of phosphorylation of the substrate ERK was similar. The region between residues 425 and 467 (termed a ‘kinase repression domain’) was thought to be largely responsible for the difference in activity. A putative PEST seq ...
Characterization of Complementary DNA Encoding the Precursor for
... conserved region of the mammalian forms; a total of nine residues may be aligned, and allowing substitution of a lysine for an arginine, this yields a stretch of six of seven matches. However, analysis of an independent population of prohormone sequences by PCR demonstrated that the bonifide cGAP do ...
... conserved region of the mammalian forms; a total of nine residues may be aligned, and allowing substitution of a lysine for an arginine, this yields a stretch of six of seven matches. However, analysis of an independent population of prohormone sequences by PCR demonstrated that the bonifide cGAP do ...
(EC 2.2.1.1) active in the Calvin cycle of spinach chloroplasts
... trophic yeast Hansenula polymorpha TKL functions as a formaldehyde-accepting dihydroxyacetone synthase (DHAS) during methanol assimilation [ 19]. Comparatively little is known about the plant transketolase enzyme. Some previous studies indicated that higher plants in general might possess chloroplas ...
... trophic yeast Hansenula polymorpha TKL functions as a formaldehyde-accepting dihydroxyacetone synthase (DHAS) during methanol assimilation [ 19]. Comparatively little is known about the plant transketolase enzyme. Some previous studies indicated that higher plants in general might possess chloroplas ...
Strong association between mRNA folding strength and protein
... have reported different relations between mF and PA, and were based on predictions of local mF and not real measurements of mF. Specifically, it was shown that there is selection for weak folding at the beginning of the coding sequence [7,15], probably as strong mF in this region decreases the effic ...
... have reported different relations between mF and PA, and were based on predictions of local mF and not real measurements of mF. Specifically, it was shown that there is selection for weak folding at the beginning of the coding sequence [7,15], probably as strong mF in this region decreases the effic ...
Biology Accelerated v. 2016
... originating from living cells and capable of producing certain chemical changes in organic substances by catalytic action, as in digestion; Lipids- comprise the fats and other esters with analogous properties and constitute, with proteins and carbohydrates, the chief structural components of living ...
... originating from living cells and capable of producing certain chemical changes in organic substances by catalytic action, as in digestion; Lipids- comprise the fats and other esters with analogous properties and constitute, with proteins and carbohydrates, the chief structural components of living ...
Journal of Biotechnology Rapid and highly efficient mammalian cell
... mRNA has led to increases in the rate of genomic cleavage in some cells. For example, a mixture of Cas9 mRNA and a single species of gRNA were co-injected into mouse embryonic stem (ES) cells resulting in biallelic mutations in 95% of newborn mice (Wang et al., 2013). To make guide RNA, often a line ...
... mRNA has led to increases in the rate of genomic cleavage in some cells. For example, a mixture of Cas9 mRNA and a single species of gRNA were co-injected into mouse embryonic stem (ES) cells resulting in biallelic mutations in 95% of newborn mice (Wang et al., 2013). To make guide RNA, often a line ...
Function and specificity of 14-3-3 proteins in the regulation of
... of the Arabidopsis genome project facilitated the identi®cation of up to 15 putative 14-3-3 genes, of which 12 are also represented as EST or cDNA clones (Rosenquist et al., 2001). An exon-based alignment enabled Wu et al. (1997) to classify Arabidopsis 14-3-3 genes into two main groups; the epsilon ...
... of the Arabidopsis genome project facilitated the identi®cation of up to 15 putative 14-3-3 genes, of which 12 are also represented as EST or cDNA clones (Rosenquist et al., 2001). An exon-based alignment enabled Wu et al. (1997) to classify Arabidopsis 14-3-3 genes into two main groups; the epsilon ...
Dynamics of the cellular metabolome during human cytomegalovirus infection.
... Viral replication requires energy and macromolecular precursors derived from the metabolic network of the host cell. Despite this reliance, the effect of viral infection on host cell metabolic composition remains poorly understood. Here we applied liquid chromatography-tandem mass spectrometry to me ...
... Viral replication requires energy and macromolecular precursors derived from the metabolic network of the host cell. Despite this reliance, the effect of viral infection on host cell metabolic composition remains poorly understood. Here we applied liquid chromatography-tandem mass spectrometry to me ...
Endogenous retrovirus
Endogenous retroviruses (ERVs) are endogenous viral elements in the genome that closely resemble and can be derived from retroviruses. They are abundant in the genomes of jawed vertebrates, and they comprise up to 5–8% of the human genome (lower estimates of ~1%). ERVs are a subclass of a type of gene called a transposon, which can be packaged and moved within the genome to serve a vital role in gene expression and in regulation. Researchers have suggested that retroviruses evolved from a type of transposable gene called a retrotransposon, which includes ERVs; these genes can mutate and instead of moving to another location in the genome they can become exogenous or pathogenic. This means that all ERVs may not have originated as an insertion by a retrovirus but that some may have been the source for the genetic information in the retroviruses they resemble.