Inositol 1,3,4,5,6-Pentakisphosphate 2-Kinase
... have been proposed for higher plants (Brearley and Hanke, 2000; Stevenson-Paulik et al., 2002; Raboy, 2003). Radioisotope labeling experiments with duckweed (Spirodela polyrhiza), an aquatic plant, suggest that a lipid-independent pathway may be operational in plants. The intermediates of this phyti ...
... have been proposed for higher plants (Brearley and Hanke, 2000; Stevenson-Paulik et al., 2002; Raboy, 2003). Radioisotope labeling experiments with duckweed (Spirodela polyrhiza), an aquatic plant, suggest that a lipid-independent pathway may be operational in plants. The intermediates of this phyti ...
Identification of enzymes involved in anaerobic benzene
... microorganisms. The proteomes of benzene-, phenoland benzoate-grown cells of culture BF were compared by SDS-PAGE. A specific benzene-expressed protein band of 60 kDa, which could not be observed during growth on phenol or benzoate, was subjected to N-terminal sequence analysis. The first 31 amino a ...
... microorganisms. The proteomes of benzene-, phenoland benzoate-grown cells of culture BF were compared by SDS-PAGE. A specific benzene-expressed protein band of 60 kDa, which could not be observed during growth on phenol or benzoate, was subjected to N-terminal sequence analysis. The first 31 amino a ...
Prokaryotic orthologues of mitochondrial alternative oxidase and plastid terminal oxidase
... above) again generated the expected 511 bp product, confirming the presence of PTOX transcript (Figure 1B). Expression was also confirmed by northern analysis of total RNA, which generated a band of ca. 800 bp (Figure 1C). The A. variabilis PTOX sequence was analyzed in the upstream region of the st ...
... above) again generated the expected 511 bp product, confirming the presence of PTOX transcript (Figure 1B). Expression was also confirmed by northern analysis of total RNA, which generated a band of ca. 800 bp (Figure 1C). The A. variabilis PTOX sequence was analyzed in the upstream region of the st ...
Characterization of the binding properties of the Avian Coronavirus
... Schematic drawing of the IBV spike protein. Detection of soluble chimeric proteins derived from coronaviruses IBV-B1648 and SARS-CoV Binding of IBVS1Fc to chicken tiss ...
... Schematic drawing of the IBV spike protein. Detection of soluble chimeric proteins derived from coronaviruses IBV-B1648 and SARS-CoV Binding of IBVS1Fc to chicken tiss ...
Compartmentation of photosynthesis in cells and
... 1.40 g ml 1). The procedure employed gave pelleted samples having densities of -1.30, 1.30±1.33, 1.33±1.36, 1.36±1.40, and )1.40 g ml 1. Earlier work with the nonaqueous technique (Stocking, 1959) had shown that de-starched chloroplasts of tobacco (C3) are lighter than most other cell constituents. ...
... 1.40 g ml 1). The procedure employed gave pelleted samples having densities of -1.30, 1.30±1.33, 1.33±1.36, 1.36±1.40, and )1.40 g ml 1. Earlier work with the nonaqueous technique (Stocking, 1959) had shown that de-starched chloroplasts of tobacco (C3) are lighter than most other cell constituents. ...
Chlamydia trachomatis RNA polymerase major sigma subunit
... close phylogenetic relatives. Chlumydia is incapable of generating ATP and thus parasitizes the host ATP to sustain metabolic activity, as well as the host ribonucleoside triphosphate pool for RNA synthesis (2). Because of their unique developmental life cycle, the chlamydiae have been assigned to t ...
... close phylogenetic relatives. Chlumydia is incapable of generating ATP and thus parasitizes the host ATP to sustain metabolic activity, as well as the host ribonucleoside triphosphate pool for RNA synthesis (2). Because of their unique developmental life cycle, the chlamydiae have been assigned to t ...
A Mutation in the Anticodon of a Single tRNA Is
... were more rounded with shortened petioles, but it is distinct in that it appears to retain sensitivity to ethylene and the mutation is homozygous lethal. Surprisingly, positional cloning on the basis of 2,4-D resistance revealed that the mutation linked to the observed phenotypes was not found in an ...
... were more rounded with shortened petioles, but it is distinct in that it appears to retain sensitivity to ethylene and the mutation is homozygous lethal. Surprisingly, positional cloning on the basis of 2,4-D resistance revealed that the mutation linked to the observed phenotypes was not found in an ...
(Plant and Fungal Physiology, UMR 2847 CNRS
... to fungal biotrophic pathogens could be converted later in the infection cycle into the C6- ...
... to fungal biotrophic pathogens could be converted later in the infection cycle into the C6- ...
Tendency for Local Repetitiveness in Amino Acid Usages in Modern
... eliminated, the peaks were not observed (Figure 2(b)), while those ®les annotated as such gave rise to the peaks as shown in Figure 2(a). However, it should be emphasized that even the protein set without zinc ®nger proteins shows a smooth curve, implying the tendency of amino acid recurrence at a l ...
... eliminated, the peaks were not observed (Figure 2(b)), while those ®les annotated as such gave rise to the peaks as shown in Figure 2(a). However, it should be emphasized that even the protein set without zinc ®nger proteins shows a smooth curve, implying the tendency of amino acid recurrence at a l ...
PDF - Fred Hutchinson Cancer Research Center
... start codon. For nonsynonymous mutations, the induced amino acid change is indicated in parentheses. ...
... start codon. For nonsynonymous mutations, the induced amino acid change is indicated in parentheses. ...
... biochemist, I have taught my subject to both ‘my own’ students, and to those on allied degree schemes and pre-clinical medicine. Of course, the lines so conveniently drawn (for teaching purposes) between the different bio-disciplines are very artificial; there is far more commonality than difference ...
CMBI
... – How do the proteins encoded in genomes interact with each other to produce cells and phenotypes ? – To predict such functional interactions between proteins as there exist e.g. in metabolic pathways, signalling pathways or protein complexes ...
... – How do the proteins encoded in genomes interact with each other to produce cells and phenotypes ? – To predict such functional interactions between proteins as there exist e.g. in metabolic pathways, signalling pathways or protein complexes ...
Construction of in vivo infectious clones of PRSV P-YK and W-CI
... infect plants in Caricaceae (Purcifull et al., 1984, Yeh et al., 1984). PRSV W was first described as Watermelon mosaic virus 1 (WMV-1) infecting cucurbits (Webb & Scott, 1965), and considered for a long time as a distinct potyvirus. However, WMV-1 and PRSV isolates are serologically indistinguishab ...
... infect plants in Caricaceae (Purcifull et al., 1984, Yeh et al., 1984). PRSV W was first described as Watermelon mosaic virus 1 (WMV-1) infecting cucurbits (Webb & Scott, 1965), and considered for a long time as a distinct potyvirus. However, WMV-1 and PRSV isolates are serologically indistinguishab ...
Novel regulatory roles of cAMP receptor proteins in
... et al., 2004). Consequently, intracellular cAMP concentrations in mycobacterial cells are considered to be ~100-fold higher than those of other bacteria (Dass et al., 2008; Padh & Venkitasubramanian, 1976, 1980), but the significance of this is not known. Secretion of cAMP directly into host macroph ...
... et al., 2004). Consequently, intracellular cAMP concentrations in mycobacterial cells are considered to be ~100-fold higher than those of other bacteria (Dass et al., 2008; Padh & Venkitasubramanian, 1976, 1980), but the significance of this is not known. Secretion of cAMP directly into host macroph ...
Sequence ID: ref|WP_006700522.1
... Clone several, using one known to work to find orthologs Use sequence to design primers to clone cDNA ...
... Clone several, using one known to work to find orthologs Use sequence to design primers to clone cDNA ...
ppt - Chair of Computational Biology
... Although 30% of E.coli genes remain unidentified, enzymes are the best studied and easily identifiable class of proteins. Therefore, few new enzymes can be expected to be discovered. The metabolic map presented may be 90% complete. Implication for metabolic maps derived from automatic genome annotat ...
... Although 30% of E.coli genes remain unidentified, enzymes are the best studied and easily identifiable class of proteins. Therefore, few new enzymes can be expected to be discovered. The metabolic map presented may be 90% complete. Implication for metabolic maps derived from automatic genome annotat ...
(Human Umbilical Vein Endothelial Cells
... Products > HUVEC Transfection Reagent (Human Umbilical Vein Endothelial Cells) Altogen Biosystems offers the HUVEC Transfection Reagent among a host of 100+ cell line specific In Vitro Transfection Kits. The HUVEC Transfection Reagent is an advanced formulation of a lipid based reagent, and it has b ...
... Products > HUVEC Transfection Reagent (Human Umbilical Vein Endothelial Cells) Altogen Biosystems offers the HUVEC Transfection Reagent among a host of 100+ cell line specific In Vitro Transfection Kits. The HUVEC Transfection Reagent is an advanced formulation of a lipid based reagent, and it has b ...
The Age of the Common Ancestor of Eukaryotes and
... average over all possible combinations. It was shown that the asymptotic bias of the estimate cx can be significantly reduced if an appropriate weight function is chosen for combining the estimates of three-sequence sets (Gu 1996). The computer program, which was originally developed for nucleotide ...
... average over all possible combinations. It was shown that the asymptotic bias of the estimate cx can be significantly reduced if an appropriate weight function is chosen for combining the estimates of three-sequence sets (Gu 1996). The computer program, which was originally developed for nucleotide ...
Molecular Plant-Microbe Interactions 13:
... 1.6.1.19) activity was determined with a colorimetric reaction according to Yonaha and Toyama (1980). Glutamate decarboxylase (GAD, EC 4.1.1.15) activity was measured by the method of Fonda (1985) as modified by Vézina et al. (1988). The bacteria used to measure GAD activity were grown in a minimal ...
... 1.6.1.19) activity was determined with a colorimetric reaction according to Yonaha and Toyama (1980). Glutamate decarboxylase (GAD, EC 4.1.1.15) activity was measured by the method of Fonda (1985) as modified by Vézina et al. (1988). The bacteria used to measure GAD activity were grown in a minimal ...
Possibilities and Limitations of Genetic Engineering
... sunflower seed albumin gene has increased the sulfur amino acid content of alfalfa, and adding a endoplasmic reticulum retention signal to the transgene greatly increased the accumulation of the sunflower seed albumin in alfalfa leaves (Tabe, 1995). Maize gamma zein, which codes for a sulfur amino a ...
... sunflower seed albumin gene has increased the sulfur amino acid content of alfalfa, and adding a endoplasmic reticulum retention signal to the transgene greatly increased the accumulation of the sunflower seed albumin in alfalfa leaves (Tabe, 1995). Maize gamma zein, which codes for a sulfur amino a ...
intolerance to lactose and other dietary sugars
... FIG. 2. Diagram showing the likely mechanism of the cis-acting polymorphism in determining lactase expression in persistent and nonpersistent adults. A developmentally regulated DNA binding protein may interact with a cis-element present on one allele and not the other (due to sequence differences w ...
... FIG. 2. Diagram showing the likely mechanism of the cis-acting polymorphism in determining lactase expression in persistent and nonpersistent adults. A developmentally regulated DNA binding protein may interact with a cis-element present on one allele and not the other (due to sequence differences w ...
Cloning, Purification, and Partial Characterization of the
... coli expression vector, overexpressed and the protein was purified and antibodies were generated. The antibodies were used to demonstrate that in vivo only the processed enzyme, without the intein, could be detected. ...
... coli expression vector, overexpressed and the protein was purified and antibodies were generated. The antibodies were used to demonstrate that in vivo only the processed enzyme, without the intein, could be detected. ...
Leukaemia Section 11q23 rearrangements in leukaemia Atlas of Genetics and Cytogenetics
... leukaemia (ALL) grossly represent half cases each; myelodysplasia (MDS) in the remaining 5%; biphenotypic leukaemia at times (likely to be more frequent with more investigations); 11q23 rearrangements in treatment related leukaemias (5-10% of 11q23 cases) are found mainly following a treatment with ...
... leukaemia (ALL) grossly represent half cases each; myelodysplasia (MDS) in the remaining 5%; biphenotypic leukaemia at times (likely to be more frequent with more investigations); 11q23 rearrangements in treatment related leukaemias (5-10% of 11q23 cases) are found mainly following a treatment with ...
News release
... genomics, proteomics, drug screening and all data-generating systems that contribute to the drug discovery process, and to organize this data so that it can be searched or mined more effectively. Jerry Walker, Vice President Informatics at Amersham Biosciences, said, “We have worked closely with Cim ...
... genomics, proteomics, drug screening and all data-generating systems that contribute to the drug discovery process, and to organize this data so that it can be searched or mined more effectively. Jerry Walker, Vice President Informatics at Amersham Biosciences, said, “We have worked closely with Cim ...
Endogenous retrovirus
Endogenous retroviruses (ERVs) are endogenous viral elements in the genome that closely resemble and can be derived from retroviruses. They are abundant in the genomes of jawed vertebrates, and they comprise up to 5–8% of the human genome (lower estimates of ~1%). ERVs are a subclass of a type of gene called a transposon, which can be packaged and moved within the genome to serve a vital role in gene expression and in regulation. Researchers have suggested that retroviruses evolved from a type of transposable gene called a retrotransposon, which includes ERVs; these genes can mutate and instead of moving to another location in the genome they can become exogenous or pathogenic. This means that all ERVs may not have originated as an insertion by a retrovirus but that some may have been the source for the genetic information in the retroviruses they resemble.