Protein profiling of the dimorphic, pathogenic fungus,Penicillium
... individuals having lived or traveled in Southeast Asia. This species is unique in that it is the only dimorphic member of the genus. Dimorphism results from a process, termed phase transition, which is regulated by temperature of incubation. At room temperature, the fungus grows filamentously (mould ...
... individuals having lived or traveled in Southeast Asia. This species is unique in that it is the only dimorphic member of the genus. Dimorphism results from a process, termed phase transition, which is regulated by temperature of incubation. At room temperature, the fungus grows filamentously (mould ...
LDL
... PPAR subtypes • In mammals, PPAR family consists of three subtypes of proteins encoded by separate genes (PPARα, PPARβ, PPARγ) with varying degrees of homology. For instance, human PPARγ and Xenopus PPARγ are more closely related in terms of amino acid identity than human PPARα and human PPARγ. • ...
... PPAR subtypes • In mammals, PPAR family consists of three subtypes of proteins encoded by separate genes (PPARα, PPARβ, PPARγ) with varying degrees of homology. For instance, human PPARγ and Xenopus PPARγ are more closely related in terms of amino acid identity than human PPARα and human PPARγ. • ...
Unusual C-terminal domain of the largest subunit of RNA
... which is characterised by a heptapeptide tandem repeat sequence with the consensus: TyrSer-Pro-Thr-Ser-Pro-Ser. The consensus sequence was found to be repeated 26 or 27 times in yeast (4,5), 44 times in Drosophila (6,7) and 52 times in mouse (8,9) and hamster (6). A number of functions, based primar ...
... which is characterised by a heptapeptide tandem repeat sequence with the consensus: TyrSer-Pro-Thr-Ser-Pro-Ser. The consensus sequence was found to be repeated 26 or 27 times in yeast (4,5), 44 times in Drosophila (6,7) and 52 times in mouse (8,9) and hamster (6). A number of functions, based primar ...
Document
... process, so it is advantageous to an organism to complement dietary intake. In mammals, cholesterol production is regulated by intracellular cholesterol concentration and by the hormones glucagon and insulin. Regulation is mediated by an elegant system of transcriptional regulation of the gene e ...
... process, so it is advantageous to an organism to complement dietary intake. In mammals, cholesterol production is regulated by intracellular cholesterol concentration and by the hormones glucagon and insulin. Regulation is mediated by an elegant system of transcriptional regulation of the gene e ...
MS-Word format
... the children terms of seed (PO:0009010). Also explain why this number is not the same as you see next to the term seed (PO:0009010). Hint: There are two types of associations in the ontology: direct associations, which are directly associated to the term for which a phenotype has been characterized ...
... the children terms of seed (PO:0009010). Also explain why this number is not the same as you see next to the term seed (PO:0009010). Hint: There are two types of associations in the ontology: direct associations, which are directly associated to the term for which a phenotype has been characterized ...
Calcitonin
... Introduction Calcitonin is a 32-amino acid polypeptide hormone that is produced in humans primarily by the parafollicular (also known as C-cells) of the thyroid, and in many other animals in the ultimobranchia body. It acts to reduce blood calcium (Ca2+), opposing the effects of parathyroid hormone ...
... Introduction Calcitonin is a 32-amino acid polypeptide hormone that is produced in humans primarily by the parafollicular (also known as C-cells) of the thyroid, and in many other animals in the ultimobranchia body. It acts to reduce blood calcium (Ca2+), opposing the effects of parathyroid hormone ...
Amino Acid Sequences and Evolutionary Relationships
... Biologists claim that the greater the similarity between the amino acid sequences of two organisms, the closer their relationship. Conversely, the greater the differences, the more distant the relationship. Further, biologists have found that such biochemical evidence compares favorably with other l ...
... Biologists claim that the greater the similarity between the amino acid sequences of two organisms, the closer their relationship. Conversely, the greater the differences, the more distant the relationship. Further, biologists have found that such biochemical evidence compares favorably with other l ...
Gene Section VIP (vasoactive intestinal peptide) Atlas of Genetics and Cytogenetics
... VIP is produced in neurons within the adrenals, brain, gastrointestinal (GI) tract, heart, pituitary and pancreas (Sundler et al., 1988). VIP addition to the adrenals causes catecholamine release (Card et al., 1988). VIP expression in the suprachiasmatic nucleus of the brain is altered by light-dark ...
... VIP is produced in neurons within the adrenals, brain, gastrointestinal (GI) tract, heart, pituitary and pancreas (Sundler et al., 1988). VIP addition to the adrenals causes catecholamine release (Card et al., 1988). VIP expression in the suprachiasmatic nucleus of the brain is altered by light-dark ...
Effects of rare codon clusters on high-level expression
... major truncated species coincided with a +1 frameshift occurring at the second codon o f an AGG tandem located at positions 208-209. Co-expression o f the argU gene completely eliminated this prematurely terminated species, but did not affect the two minor molecular weight species. Interestingly, th ...
... major truncated species coincided with a +1 frameshift occurring at the second codon o f an AGG tandem located at positions 208-209. Co-expression o f the argU gene completely eliminated this prematurely terminated species, but did not affect the two minor molecular weight species. Interestingly, th ...
sequence alignments
... Point-accepted mutations Dayoff (1960’s) calculated substitution probabilities from alignments of highly similar protein families All the PAM data come from closely related proteins (>85% amino acid identity). The PAM1 is the matrix calculated from comparisons of sequences with no more than 1% diver ...
... Point-accepted mutations Dayoff (1960’s) calculated substitution probabilities from alignments of highly similar protein families All the PAM data come from closely related proteins (>85% amino acid identity). The PAM1 is the matrix calculated from comparisons of sequences with no more than 1% diver ...
Word
... valine in the culture medium while accumulating pyruvate, 2-ketobutyrate and 2-aminobutyrate ...
... valine in the culture medium while accumulating pyruvate, 2-ketobutyrate and 2-aminobutyrate ...
Document
... column) while gij are the frequencies observed in a database (e.g., the complete alignment, sequence family, or complete sequence database) • and are scaling factors to weigh the f and g contributions: if equals the number of sequences in profile, and =1 (preceding slide), then the influence ...
... column) while gij are the frequencies observed in a database (e.g., the complete alignment, sequence family, or complete sequence database) • and are scaling factors to weigh the f and g contributions: if equals the number of sequences in profile, and =1 (preceding slide), then the influence ...
Sample & Assay Technologies QIAgenes E. coli
... provided by cells carrying the pLysE plasmid, where expression levels of lysozyme are higher than those carrying the pLysS plasmid. Adding the inducer IPTG (~1 mM) or using autoinduction media overcomes the inhibitory effect of the lysozyme protein present in the cell. Please note that the QIAgenes ...
... provided by cells carrying the pLysE plasmid, where expression levels of lysozyme are higher than those carrying the pLysS plasmid. Adding the inducer IPTG (~1 mM) or using autoinduction media overcomes the inhibitory effect of the lysozyme protein present in the cell. Please note that the QIAgenes ...
Analysis of Binary Relations and Hierarchies of Enzymes in the
... includes genes, gene products, chemical compounds, enzyme reactions and metabolic pathway diagrams. By automatically mapping the enzymes of a specic organism on the pathway diagrams, it becomes possible to visualize the characteristic features of the organismspecic metabolic pathways. With the aid ...
... includes genes, gene products, chemical compounds, enzyme reactions and metabolic pathway diagrams. By automatically mapping the enzymes of a specic organism on the pathway diagrams, it becomes possible to visualize the characteristic features of the organismspecic metabolic pathways. With the aid ...
Jhe in Gryllus assimilis: Cloning, sequence
... To determine whether the JHE coding sequence differs between crickets selected for high or low hemolyph JHE activity, total RNA was isolated individually from four high activity and four low activity G. assimilis using Trizol (Invitrogen). RNA was treated with DNAase, converted into cDNA using 200u ...
... To determine whether the JHE coding sequence differs between crickets selected for high or low hemolyph JHE activity, total RNA was isolated individually from four high activity and four low activity G. assimilis using Trizol (Invitrogen). RNA was treated with DNAase, converted into cDNA using 200u ...
NON-CANONICAL TRANSCRIPTION INITIATION: THE EXPANDING
... In the cell, the nicotinamide ribonucleoside moiety can be removed by a Nudix enzyme. Nudix enzymes are pyrophosphohydrolases that catalyze the breakage of pyrophosphate bonds in a range of substrates (McLennan, 2013). Consistently, deletion of nudC resulted in about a twofold increase in the abunda ...
... In the cell, the nicotinamide ribonucleoside moiety can be removed by a Nudix enzyme. Nudix enzymes are pyrophosphohydrolases that catalyze the breakage of pyrophosphate bonds in a range of substrates (McLennan, 2013). Consistently, deletion of nudC resulted in about a twofold increase in the abunda ...
That have been aligned so that homologous residues are arranged
... Amino-acid sequence alignments are easier to carry out and less ambiguous than nucleotide alignments. A typical approach is to carry out the alignment at the aminoacid level and use it to generate a corresponding nucleotide sequence alignment, which can then be analyzed as usual. Numerous computer p ...
... Amino-acid sequence alignments are easier to carry out and less ambiguous than nucleotide alignments. A typical approach is to carry out the alignment at the aminoacid level and use it to generate a corresponding nucleotide sequence alignment, which can then be analyzed as usual. Numerous computer p ...
Fulltext - Jultika
... suggest that Tyr94 and Trp311 play critical roles in catalysis. A pocket was found in the structure extending away from the catalytic site that can accommodate fatty acyl chains up to 16 carbons. An acyl carrier protein (ACP) binding site was also suggested. To study the physiological function of mo ...
... suggest that Tyr94 and Trp311 play critical roles in catalysis. A pocket was found in the structure extending away from the catalytic site that can accommodate fatty acyl chains up to 16 carbons. An acyl carrier protein (ACP) binding site was also suggested. To study the physiological function of mo ...
Optimizing cofactor availability for the production of recombinant
... Despite the high conservation of the heme biosynthesis pathway, distinct differences were found among the different species. In E. coli, formation of 5-aminolevulinic acid (ALA) by the HEM1encoded ALA synthase was described as rate-limiting step [10], whereas in S. cerevisiae the HEM2- and HEM3-enco ...
... Despite the high conservation of the heme biosynthesis pathway, distinct differences were found among the different species. In E. coli, formation of 5-aminolevulinic acid (ALA) by the HEM1encoded ALA synthase was described as rate-limiting step [10], whereas in S. cerevisiae the HEM2- and HEM3-enco ...
APC5 Antibody
... to be essential for the normal progression of mitosis. APC5 is a highly conserved component of the anaphase promoting complex/cyclosome (APC/C), a cell cycle-regulated E3 ubiquitin ligase that controls progression through mitosis and the G1 phase of the cell cycle. APC/C is responsible for degrading ...
... to be essential for the normal progression of mitosis. APC5 is a highly conserved component of the anaphase promoting complex/cyclosome (APC/C), a cell cycle-regulated E3 ubiquitin ligase that controls progression through mitosis and the G1 phase of the cell cycle. APC/C is responsible for degrading ...
Virology Journal
... and eiMSLS, respectively, share significant sequence similarity to known protein sequences contained in the GenBank nr/nt database (Table 1). Of the ORFs with significant sequence similarity to sequences in GenBank, putative functions could only be assigned to 21 out of 40 (53%), 21 out of 37 (57%) ...
... and eiMSLS, respectively, share significant sequence similarity to known protein sequences contained in the GenBank nr/nt database (Table 1). Of the ORFs with significant sequence similarity to sequences in GenBank, putative functions could only be assigned to 21 out of 40 (53%), 21 out of 37 (57%) ...
Colicins produced by the Escherichia fergusonii strains closely
... (A71T) in colicin E1 protein (Fig. 1). pColE1-EF3 imm and kil gene sequences were identical to those of pColE1-EF43. Consistent with this ¢nding, E. coli strain TOP10FP with pDS455 was immune to colicin E1 produced by EF3 strain and vice versa (Table 1). The size of pColE1-EF3 was determined to be a ...
... (A71T) in colicin E1 protein (Fig. 1). pColE1-EF3 imm and kil gene sequences were identical to those of pColE1-EF43. Consistent with this ¢nding, E. coli strain TOP10FP with pDS455 was immune to colicin E1 produced by EF3 strain and vice versa (Table 1). The size of pColE1-EF3 was determined to be a ...
Module 4 Notes full - Mark Rothery`s Biology
... Pyruvate marks the end of glycolysis, the first stage of respiration. In the presence of oxygen pyruvate enters the mitochondrial matrix to proceed with aerobic respiration, but in the absence of oxygen it is converted into lactate (in animals and bacteria) or ethanol (in plants and fungi). These ar ...
... Pyruvate marks the end of glycolysis, the first stage of respiration. In the presence of oxygen pyruvate enters the mitochondrial matrix to proceed with aerobic respiration, but in the absence of oxygen it is converted into lactate (in animals and bacteria) or ethanol (in plants and fungi). These ar ...
Cloning
... somatic cell nucleus.[9] The oocyte will react on the somatic cell nucleus, the same way it would on sperm cells.[9] The process of cloning a particular farm animal using SCNT is relatively the same for all animals. The first step is to collect the somatic cells from the animal that will be cloned. ...
... somatic cell nucleus.[9] The oocyte will react on the somatic cell nucleus, the same way it would on sperm cells.[9] The process of cloning a particular farm animal using SCNT is relatively the same for all animals. The first step is to collect the somatic cells from the animal that will be cloned. ...
Genomic structure, chromosomal localization, and conserved alternative splice forms of thrombopoietin
... human and pig. The homology is substantially greater in the amino terminal half of TPO (epo homologous domain). This domain is 8 1 % to 85% identical between any two species, whereas the carboxyl half is only 57% to 67% identical. A conserved dibasic amino acid motif that could represent a protease ...
... human and pig. The homology is substantially greater in the amino terminal half of TPO (epo homologous domain). This domain is 8 1 % to 85% identical between any two species, whereas the carboxyl half is only 57% to 67% identical. A conserved dibasic amino acid motif that could represent a protease ...
Endogenous retrovirus
Endogenous retroviruses (ERVs) are endogenous viral elements in the genome that closely resemble and can be derived from retroviruses. They are abundant in the genomes of jawed vertebrates, and they comprise up to 5–8% of the human genome (lower estimates of ~1%). ERVs are a subclass of a type of gene called a transposon, which can be packaged and moved within the genome to serve a vital role in gene expression and in regulation. Researchers have suggested that retroviruses evolved from a type of transposable gene called a retrotransposon, which includes ERVs; these genes can mutate and instead of moving to another location in the genome they can become exogenous or pathogenic. This means that all ERVs may not have originated as an insertion by a retrovirus but that some may have been the source for the genetic information in the retroviruses they resemble.