Phenotypic and Genotypic Comparisons among Strains of the
... Serological grouping of virulent and avirulent strains of A . viriduns indicates that virulent strains possess a special antigen absent from avirulent strains (20). Steenbergen et al. (20) emphasize, however, that the presence or absence of the virulence antigen is not in itself justification for di ...
... Serological grouping of virulent and avirulent strains of A . viriduns indicates that virulent strains possess a special antigen absent from avirulent strains (20). Steenbergen et al. (20) emphasize, however, that the presence or absence of the virulence antigen is not in itself justification for di ...
Identification and characterization of the ergochrome gene cluster in
... The yellow ergochromes are dimers of tetrahydroxanthone units [7]. Four different xanthone derivatives were described as ergochrome units in C. purpurea and all possible combinations of two of these units occur in nature [8]. Their concentration in the sclerotia is considerably higher (5 g/kg) than ...
... The yellow ergochromes are dimers of tetrahydroxanthone units [7]. Four different xanthone derivatives were described as ergochrome units in C. purpurea and all possible combinations of two of these units occur in nature [8]. Their concentration in the sclerotia is considerably higher (5 g/kg) than ...
The Arabidopsis ABHD11 Mutant Accumulates
... caused the accumulation of the polar lipids in leaves, which in turn promoted a higher growth rate compared with wild-type plants. ...
... caused the accumulation of the polar lipids in leaves, which in turn promoted a higher growth rate compared with wild-type plants. ...
X(0,25) - IFSC-USP
... Genetic Code Redundancy • Because 64 possible three “letter” codons can be created from four nucleotides and only 22 amino acids are encoded by them the code is redundant. • It was ...
... Genetic Code Redundancy • Because 64 possible three “letter” codons can be created from four nucleotides and only 22 amino acids are encoded by them the code is redundant. • It was ...
Controlling subcellular delivery to optimize
... nature and allow for a more sophisticated approach to drug delivery. Harnessing different methods of targeting multiple organelles in a cell will lead to better drug delivery and improvements in disease therapy. ...
... nature and allow for a more sophisticated approach to drug delivery. Harnessing different methods of targeting multiple organelles in a cell will lead to better drug delivery and improvements in disease therapy. ...
this PDF file
... Cucumber mosaic virus strain soybean (CMV-S) is one of economically important virus infecting soybean in Indonesia. However, it is very few information related with the CMV-S Indonesia isolates. Thus, the aim of present work is to detect and identify CMV-S isolates from different origin. Leaf sample ...
... Cucumber mosaic virus strain soybean (CMV-S) is one of economically important virus infecting soybean in Indonesia. However, it is very few information related with the CMV-S Indonesia isolates. Thus, the aim of present work is to detect and identify CMV-S isolates from different origin. Leaf sample ...
... enzyme are shown to the right, along with some amino acid sidechains from the enzyme (shown in bold). The initial rate of product formation was measured in the absence of either inhibitor, and in the presence of equal amounts (1 µM) of either inhibitor. The data are plotted on both velocity curves a ...
Immunoinformatics Study of gp120 of Human Immunodeficiency
... neutralize HIV infection, however, these antibodies are mostly type-specific, and do not possess the potential of broad neutralization (12). Variation events in the V2 and V3 regions of the envelope glycoprotein increase the ability of the viral strains to infect different cell types (13). Besides, ...
... neutralize HIV infection, however, these antibodies are mostly type-specific, and do not possess the potential of broad neutralization (12). Variation events in the V2 and V3 regions of the envelope glycoprotein increase the ability of the viral strains to infect different cell types (13). Besides, ...
Ammonium transport in Escherichia coli: localization and nucleotide
... chloroform extractions, ethanol precipitation of DNA, restriction digests, DNA ligation, bacterial transformation and agarose gel electrophoresis were done according to standard protocols as described by Sambrook et al. (1989). Construction of' unidirectional deletions of the Amt-complementing subcl ...
... chloroform extractions, ethanol precipitation of DNA, restriction digests, DNA ligation, bacterial transformation and agarose gel electrophoresis were done according to standard protocols as described by Sambrook et al. (1989). Construction of' unidirectional deletions of the Amt-complementing subcl ...
國立清華大學 - 罕見疾病基金會
... the most conserved core subunits of mitochondrial complex I, plays an important role in the oxidative phosphorylation system (OXPHOS). This protein binds one iron-sulfur cluster N2 (tetranuclear) which is the terminal redox center in the electron transport process of complex I. Three types of mutati ...
... the most conserved core subunits of mitochondrial complex I, plays an important role in the oxidative phosphorylation system (OXPHOS). This protein binds one iron-sulfur cluster N2 (tetranuclear) which is the terminal redox center in the electron transport process of complex I. Three types of mutati ...
Protein digestion in poultry – the value of an
... important e.g. phytate, fibre, refractory proteins and starch • Enzymes can degrade antinutrients such as trypsin inhibitors and phytate • INHERENT DIGESTIBILITY OF FOCAL NUTRIENTS is absolutely central to the magnitude and consistency of enzyme effects (Cowieson, 2010) – Xylanase, protease and phyt ...
... important e.g. phytate, fibre, refractory proteins and starch • Enzymes can degrade antinutrients such as trypsin inhibitors and phytate • INHERENT DIGESTIBILITY OF FOCAL NUTRIENTS is absolutely central to the magnitude and consistency of enzyme effects (Cowieson, 2010) – Xylanase, protease and phyt ...
PDF - Andrew Rambaut
... multiple artificially derived mutations, and both found evidence that most interactions among deleterious mutations exhibited positive epistasis. This has important implications for the understanding of the evolution of sex, suggesting that RNA recombination is not an adaptation to purge deleterious ...
... multiple artificially derived mutations, and both found evidence that most interactions among deleterious mutations exhibited positive epistasis. This has important implications for the understanding of the evolution of sex, suggesting that RNA recombination is not an adaptation to purge deleterious ...
Tuning Biphenyl Dioxygenase for Extended Substrate Specificity
... 1986; Bopp, 1986). Due to its broad substrate specificity, it was used as the starting template for generating variant enzymes that can act on an extended set of PCB congeners. Because substrate specificity is determined mainly by the bphA region, only this gene fragment was subjected to directed ev ...
... 1986; Bopp, 1986). Due to its broad substrate specificity, it was used as the starting template for generating variant enzymes that can act on an extended set of PCB congeners. Because substrate specificity is determined mainly by the bphA region, only this gene fragment was subjected to directed ev ...
Pattern searches for the identification of putative lipoprotein genes in
... most N-terminal MSD and also the number of additional MSDs. Those sequences where either an MSD was clearly absent or the most N-terminal MSD clearly extended beyond the lipobox cysteine (e.g. sequences likely to direct insertion of integral membrane proteins) were considered possible falsepositives ...
... most N-terminal MSD and also the number of additional MSDs. Those sequences where either an MSD was clearly absent or the most N-terminal MSD clearly extended beyond the lipobox cysteine (e.g. sequences likely to direct insertion of integral membrane proteins) were considered possible falsepositives ...
Chloroplast phosphoglycerate kinase from Euglena gracilis
... not shown). These findings strongly suggest that two very similar isoforms of the chloroplast PGK were purified from total Euglena cells, which can be separated on Mono Q. Both proteins had identical N-terminal amino acid sequences as determined by N-terminal protein sequencing (Table 2). The amino ac ...
... not shown). These findings strongly suggest that two very similar isoforms of the chloroplast PGK were purified from total Euglena cells, which can be separated on Mono Q. Both proteins had identical N-terminal amino acid sequences as determined by N-terminal protein sequencing (Table 2). The amino ac ...
SQA Higher Biology Unit 1 Cell Biology
... Eukaryotic unicells are divided into compartments called organelles that carry out different functions. Eukaryotic multicellular organisms are composed of many types of cell that are differentiated to carry out distinct functions (Figure 1.1). The organism therefore works more efficiently. In other ...
... Eukaryotic unicells are divided into compartments called organelles that carry out different functions. Eukaryotic multicellular organisms are composed of many types of cell that are differentiated to carry out distinct functions (Figure 1.1). The organism therefore works more efficiently. In other ...
The Glycerophosphoryl Diester
... transposon insertion lines (Kuromori et al. 2004, Kuromori et al. 2006). In the mutant line 15-1096-1, tip growth in nearly all root hair cells was blocked due to a rupture at the tip (Fig. 1A). However, bulges were observed at the appropriate positions in the trichoblasts, indicating normal root ha ...
... transposon insertion lines (Kuromori et al. 2004, Kuromori et al. 2006). In the mutant line 15-1096-1, tip growth in nearly all root hair cells was blocked due to a rupture at the tip (Fig. 1A). However, bulges were observed at the appropriate positions in the trichoblasts, indicating normal root ha ...
Identification and Characterization of Two Temperature
... presence of the adiS gene in all 42 serotype 2 and 9 S. suis strains tested. In 9 of these 42 strains, selected randomly, we confirmed expression of the AdiS protein, homologous to SAGP, by immunoblot analysis using a specific antiserum against the SAGP of S. pyogenes. In all strains AD activity was ...
... presence of the adiS gene in all 42 serotype 2 and 9 S. suis strains tested. In 9 of these 42 strains, selected randomly, we confirmed expression of the AdiS protein, homologous to SAGP, by immunoblot analysis using a specific antiserum against the SAGP of S. pyogenes. In all strains AD activity was ...
Inquiry into Life Twelfth Edition
... • If amino acid supply low, ribosomes stall at the tandem tryptophan codons in the trp leader • trp leader being synthesized as stalling occurs, stalled ribosome will influence the way RNA folds – Prevents formation of a hairpin – This is part of the transcription termination signal which causes att ...
... • If amino acid supply low, ribosomes stall at the tandem tryptophan codons in the trp leader • trp leader being synthesized as stalling occurs, stalled ribosome will influence the way RNA folds – Prevents formation of a hairpin – This is part of the transcription termination signal which causes att ...
A point mutation in the Ch3 domain of human IgG3... secretion without affecting antigen specificity
... shown in Fig. 2. Control chIgG1 was efficiently secreted into the supernatant at both 24 and 48 h following transfection whereas the original chIgG3 (Mut IgG3) was not secreted although it was detectable in cell-lysates at 24 h posttransfection (Fig. 2A and B), consistent with previous results. This ...
... shown in Fig. 2. Control chIgG1 was efficiently secreted into the supernatant at both 24 and 48 h following transfection whereas the original chIgG3 (Mut IgG3) was not secreted although it was detectable in cell-lysates at 24 h posttransfection (Fig. 2A and B), consistent with previous results. This ...
The Science of Transgenics
... Genetic engineering is the basic tool set of biotechnology Genetic engineering involves: Isolating genes Modifying genes so they function better Preparing genes to be inserted into a new species Developing transgenes NDSU ...
... Genetic engineering is the basic tool set of biotechnology Genetic engineering involves: Isolating genes Modifying genes so they function better Preparing genes to be inserted into a new species Developing transgenes NDSU ...
Isolation of the b-tubulin Gene From Yeast and Demonstration of its Essential Function in vivo.
... al., 1980). A similar result has recently been obtained with S. cerevisiae strains resistant to the benomyl derivative MBC (P. Baum and J. Thorner, unpublished data). The Aspergillus results suggested to us that if benomyl-resistant yeast strains are /3-tubulin mutants as well, the resistance phenot ...
... al., 1980). A similar result has recently been obtained with S. cerevisiae strains resistant to the benomyl derivative MBC (P. Baum and J. Thorner, unpublished data). The Aspergillus results suggested to us that if benomyl-resistant yeast strains are /3-tubulin mutants as well, the resistance phenot ...
Gene Section PKM2 (pyruvate kinase isoenzyme type M2) in Oncology and Haematology
... metabolites. High fructose 1,6-P2 levels induce the association of the inactive dimeric form of M2-PK to the highly active tetrameric form. When FBP levels drop below a critical value the tetrameric form dissociates to the dimeric form. Dimerization of M2PK is induced by direct interaction with diff ...
... metabolites. High fructose 1,6-P2 levels induce the association of the inactive dimeric form of M2-PK to the highly active tetrameric form. When FBP levels drop below a critical value the tetrameric form dissociates to the dimeric form. Dimerization of M2PK is induced by direct interaction with diff ...
XIST
... Models of Tsix-mediated repression of Xist. (A) Tsix DNA sequence itself could function as a long-range silencer to repress or block the transcription of the Xist gene. (B) Transcription of Xist may be prohibited by the processivity of RNA polymerase in the antisense orientation. As RNA polymerase ...
... Models of Tsix-mediated repression of Xist. (A) Tsix DNA sequence itself could function as a long-range silencer to repress or block the transcription of the Xist gene. (B) Transcription of Xist may be prohibited by the processivity of RNA polymerase in the antisense orientation. As RNA polymerase ...
Endogenous retrovirus
Endogenous retroviruses (ERVs) are endogenous viral elements in the genome that closely resemble and can be derived from retroviruses. They are abundant in the genomes of jawed vertebrates, and they comprise up to 5–8% of the human genome (lower estimates of ~1%). ERVs are a subclass of a type of gene called a transposon, which can be packaged and moved within the genome to serve a vital role in gene expression and in regulation. Researchers have suggested that retroviruses evolved from a type of transposable gene called a retrotransposon, which includes ERVs; these genes can mutate and instead of moving to another location in the genome they can become exogenous or pathogenic. This means that all ERVs may not have originated as an insertion by a retrovirus but that some may have been the source for the genetic information in the retroviruses they resemble.